• Journal of Veterinary Clinics
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• v.36 no.3
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• pp.139-144
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• 2019

Intervertebral disk disease is a common neurological disease in dogs. In veterinary neurology clinics, the incidence of intervertebral disk disease and the spinal cord compression rate of the disk are well documented. However, the direction of herniation of the intervertebral disk is relatively less known on Magnetic resonance imaging (MRI). Therefore, this study systematically investigated and analyzed the intervertebral disk disease in dogs. Especially, classification according to the direction of herniation of the disk, the degree of pre - treatment neurological manifestation, and the compression rate was investigated. In addition, the treatment methods of patients who received treatment after visiting the Chonbuk animal medical center (CAMC) were divided into pharmacological treatment and surgical treatment. The location of intervertebral disc in spinal canal was mainly ventral (69.9%), and when the prognosis was evaluated according to the compression rate of the spinal cord, the prognosis of the surgical treatment (72.7%) was more effective than the non - surgical medication (40.6%). Surgical treatment (83.3%) was more successful than nonsurgical medication (60%), especially in cases of severe spinal cord compression (> 50%). In this study, surgical treatment was more effective when the neurological symptoms were severe or the spinal cord compression rate was high.

• Journal of Korean Dental Science
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• v.15 no.1
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• pp.31-50
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• 2022

Purpose: This study was to investigate the types of imaging modalities, analytical methods for age estimation, and the age of the subjects in research on age estimation using dental radiography through a scoping review, and to investigate the overall trends in age estimation studies. Materials and Methods: A scoping review was designed according to the Arksey and O'Malley guidelines and the Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) statement. Three electronic databases were used as search sources (Medline, Embase, and Cochrane Library). Studies were classified according to the three main components of the research question. "What are the imaging modalities, analytical methods, and target age in dental imaging-based age estimation studies?" Result: The final 198 studies were selected by two reviewers. The most common imaging modality used in studies was panoramic radiography (69.7%), and studies using cone-beam computed tomography have increased over time. Analytical methods for age estimation were 62.6% in studies based on tooth development and 26.3% in studies using pulp/tooth ratio. The subject age was 27.8% for children and 27.3% for adults. Studies conducted in all age groups comprised the smallest category (5.2%). Conclusion: Panoramic radiography has been the most used types of imaging modalities for age estimation, and the most common analytical method was analysis of tooth development. Most studies targeted specific ages, and very few involved all age groups. Dental age estimation studies should be carried out with appropriate consideration of the imaging modality that is analyzed, the methods that are used, and the age that is targeted.

• Journal of Microbiology and Biotechnology
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• v.33 no.2
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• pp.211-218
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• 2023

A cryptic plasmid (pTH32) was characterized from Tetragenococcus halophilus 32, an isolate from jeotgal, Korean traditional fermented seafood. pTH32 is 3,198 bp in size with G+C content of 35.84%, and contains 4 open reading frames (ORFs). orf1 and orf2 are 456 bp and 273 bp in size, respectively, and their translation products showed 65.16% and 69.35% similarities with RepB family plasmid replication initiators, respectively, suggesting the rolling-circle replication (RCR) mode of pTH32. orf3 and orf4 encodes putative hypothetical protein of 186 and 76 amino acids, respectively. A novel Tetragenococcus-Escherichia coli shuttle vector, pMJ32E (7.3 kb, Em^r), was constructed by ligation of pTH32 with pBluescript II KS(+) and an erythromycin resistance gene (ErmC). pMJ32E successfully replicated in Enterococcus faecalis 29212 and T. halophilus 31 but not in other LAB species. A pepA gene, encoding aminopeptidase A (PepA) from T. halophilus CY54, was successfully expressed in T. halophilus 31 using pMJ32E. The transformant (TF) showed higher PepA activity (49.8 U/mg protein) than T. halophilus 31 cell (control). When T. halophilus 31 TF was subculturd in MRS broth without antibiotic at 48 h intervals, 53.8% of cells retained pMJ32E after 96 h, and only 2.4% of cells retained pMJ32E after 14 days, supporting the RCR mode of pTH32. pMJ32E could be useful for the genetic engineering of Tetragenococcus and Enterococcus species.

• Microbiology and Biotechnology Letters
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• v.51 no.2
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• pp.157-166
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• 2023

Three lactic acid bacteria (LAB) producing exopolysaccharides (EPSs) were isolated from Pa (green onion)-kimchi, and identified as Weissella confusa (SKP 173), Weissella cibaria (SKP 182), and Leuconostoc citreum (SKP 281), respectively by 16S rRNA gene sequencing. The yields of EPS were 21.27, 18.53, and 15.4 g/l for EPS from SKP 173, 182, and 281, respectively when grown in MRS broth containing sucrose (5%, w/v). Total sugar contents were 64.39, 62.84, and 65.16% (w/w) for EPS from SKP 173, 182, and 281, respectively while the protein contents were 0.33, 0.31, and 0.25% (w/w), respectively. EPSs from W. confusa SKP 173 and W. cibaria SKP 182 contained glucose only but EPS from L. citreum SKP 281 contained glucose and glucitol. Viscosities of the 2% (w/w) freeze-dried EPS solution were 9.60, 8.00, and 8.20 centipoise (cP) for EPS from SKP 173, 182, and 281, respectively. Viscosities of culture grown in MRS broth with 5% sucrose (no glucose) were 92.98, 57.19, and 18.8 cP, respectively. The average molecular weights of EPSs were larger than 2 × 10⁷ Da. Fourier transform infrared spectroscopy (FT-IR) analyses of EPSs showed typical carbohydrate peaks, suggesting that the EPSs consisted of pyranose saccharides with α-(1,6) and α-(1,3) glycosidic linkages. L. citreim SKP 281 was used as the starter for yogurt fermentation, and EPS production was confirmed.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.363-363
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• 2022

LID 시설에 대한 모니터링은 인력을 활용한 실강우 모니터링을 진행하고 있으나 LID 시설은 소규모 분산형시설로서 인력을 동원한 식생고사, 강우시 모니터링, 현장답사 등 꾸준한 시설확인에 한계가 있으며, LID 시설을 조성한 이후 적정한 유지관리 방법(주기, 빈도, 항목 등)을 인지하지 못하여 막힘현상, 효율저하, 식물고사 등의 문제가 발생한다. 따라서 본연구에서는 딥러닝 분석을 활용하여 강우시 강우모니터링 자료와 LID 시설 내 센서를 통해 측정된 자료를 통해 침투도랑 내 유입수 성상에 대한 예측분석을 수행하였다. 심지 내 LID 시설에 유입되는 오염물질을 예측을 위한 딥러닝 분석을 위해 과거 실강우시 모니터링 자료(TSS, COD, TN, TP)와 대기센서(대기습도, 대기온도, 강수량, 미세먼지) 데이터를 활용하여 딥러닝 모델에 대한 적용가능성 평가를 수행하였다. 측정항목에 대한 상관성 분석을 수행하였으며, 딥러닝 모델은 Tenser Flow를 이용하여 DNN(Deep Neural Network)모델을 활용하여 분석하였다. DNN 모델에 대한 MSE값은 0.31로 분석되었으며, TSS에 대한 평균 50.6mg/L로 분석되었으며, COD 평균 98.7 mg/L로 나타났다. TN의 평균 2.21 mg/L로 분석되었으며, TP 평균 0.67 mg/L로 나타났다. 상관계수분석결과 TSS는 0.53로 분석되었으며, TN과 TP의 상관계수는 0.10, 0.56으로 나타났다. COD의 상관계수는 0.63으로 TSS와 COD, TP에 대한 예측이 된 것으로 분석되었다. 딥러닝을 통한 LID 시설 내 농도변화 예측시 강우시 센서데이터 값은 조밀해야하며 오염물질 농도와 상관성이 높은 항목들에 대해 계측과 실강우 모니터링 자료를 축적하여 미래에 대한 활용성을 높여야 한다.

• Korean Journal of Food Science and Technology
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• v.42 no.3
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• pp.329-334
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• 2010

Escherichia coli O157:H7 causes hemolytic uremic syndrome and hemorrhagic colitis in humans. The objectives of this study were to produce monoclonal antibody(MAb) against E. coli O157:H7 and to develop an enzyme linked immunosorbent assay(ELISA) for the rapid detection of E. coli O157:H7 in agri-stockbreeding. The characterization of MAb produced from hybridoma cell (HKEC 4G8-5) was validated by ELISA and Western blot. The produced MAb was specific to E. coli O157:H7 and showed weak cross-reaction to Staphylococcus aureus. The detection limit of ELISA based on 4G8-5 MAb was $10^5\;cell/mL$. Although the ELISA could not detect E. coli O157:H7 in the meat and sprout samples inoculated with $1{\times}10^1\;cell$/10 g without enrichment, the same samples after enrichment for 6 hr were confirmed to be positive by ELISA. These results indicated that the ELISA combined with short enrichment (6 hr) is useful tool for rapid screening of E. coli O157:H7 in various samples.

• Korean Journal of Optics and Photonics
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• v.11 no.6
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• pp.418-422
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• 2000

In this paper, we have modeled and fabricated a mutimode interference (MMI) $1\times4$ optical power splitter using finite-difference beam propagation method and $Ag^+-Na^+$ ion-exchanged method in BK7 glass. The power splitting ratio of the fabricated MMI $1\times4$ optical power splitter shows 0.46 dB..46 dB.

• Current Optics and Photonics
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• v.4 no.1
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• pp.44-49
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• 2020

In this paper, a new method is presented to measure the refractive index of single plain glass or multilayered materials, based on a laser frequency-shifted confocal feedback microscope. Combining the laser frequency-shifted feedback technique and the confocal effect, the method can attain high axial-positioning accuracy, stability and sensitivity. Measurements of different samples are given, including N-BK7 glass, Silica plain glass, and a microfluidic chip with four layers. The results for N-BK7 glass and Silica plain glass show that the measurement uncertainty in the refractive index is better than 0.001. Meanwhile, the feasibility of this method for multilayered materials is tested. Compared to conventional methods, this system is more compact and has less difficulty in sample processing, and thus is promising for applications in the area of refractive-index measurement.

• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3621-3628
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• 2013

Novel 5'-deoxythreosyl purine phosphonic acid analogues containing a 2'-electropositive moiety such as fluorine atom, were designed and synthesized from commercially available 1,3-dihydroxy acetone. Condensation successfully proceeded from a glycosyl donor 6 under Vorbr$\ddot{u}$ggen conditions and cross-metathesis gave the desired phosphonate analogues 7a, 7b, 17a and 17b. The synthesized nucleoside phosphonic acid analogues 13, 16, 23, 26, 28 were subjected to antiviral screening against HIV-1. The bis(SATE) adenine analogue 28 exhibited significant in vitro activities against HIV-1.

• Korean Journal of Optics and Photonics
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• v.3 no.4
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• pp.217-221
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• 1992

A micro lens system was designed for the modulator in optical fiber communication. One was the collimating lens which transferred the diffracted beam from optical fiber to the modulator. The other was the coupling lens which connected the modulated collimating beam to the optical fiber. The light source was He-Ne laser beam. The lens would be made of optical glass BK-7. We determined the tolerance of curvature radius, thickness and conic constant.