• Title/Summary/Keyword: BK185

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Fungal Diversity in Composting Process of Pig Manure and Mushroom Cultural Waste Based on Partial Sequence of Large Subunit rRNA

  • Cho, Kye-Man;Kwon, Eun-Ju;Kim, Sung-Kyum;Kambiranda, Devaiah M;Math, Reukaradhya K;Lee, Young-Han;Kim, Jung-Ho;Yun, Han-Dae;Kim, Hoon
    • Journal of Microbiology and Biotechnology
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    • v.19 no.8
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    • pp.743-748
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    • 2009
  • Fungal diversity during composting was investigated by culture-independent rDNA sequence analysis. Composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (Hazaka system), and samples were collected at various stages. Based on partial sequence analysis of large subunit (LSU) ribosomal RNA (rRNA) and sequence identity values, a total of 12 different fungal species were found at six sampling sites; Geotrichum sp., Debaryomyces hansenii, Monographella nivalis, Acremonium strictum, Acremonium alternatum, Cladosporium sphaerospermum, Myriangium durosai, Pleurotus eryngii, Malassezia globosa, Malassezia restricta, Rhodotorula glutinis, and Fusarium sporotrichioides. Geotrichum sp. of the class Saccharomycetes was the most predominant fungal species throughout the composting process (185 out of a total of 236 identified clones, or 78.4%), followed by Acremonium strictum (7.6%), Monographella nivalis (5.1%), and Pleurotus eryngii (3.8%). The prevalence of Geotrichum sp. was the lowest (61.1%) at the beginning of composting, and then gradually increased to 92.5% after 10 days of composting.

Analysis of nucleotide sequence of a novel plasmid, pILR091, from Lactobacillus reuteri L09 isolated from pig

  • Lee, Deog-Yong;Kang, Sang-Gyun;Rayamajhi, Nabin;Kang, Milan;Yoo, Han Sang
    • Korean Journal of Veterinary Research
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    • v.48 no.4
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    • pp.441-449
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    • 2008
  • The genus Lactobacillus is the largest of the genera included in lactic acid bacteria and is associated with mucosal membranes of human and animal. Only a few Lactobacillus plasmid-encoded functions have been discovered and used. In this study, a novel plasmid (pILR091) was isolated from a wild L. reuteri isolated from pig and described the characteristics of its replicons, genetic organization, and relationship with other plasmids. After digestion of the plasmid, pILR091, with SalI, plasmid DNA was cloned into the pQE-30Xa vector and sequenced. The complete sequence was confirmed by the sequencing of PCR products and analyzed with the Genbank database. The isolate copy number and stability were determined by quantitative-PCR. The complete sequence of L. reuteri contained 7,185 nucleotides with 39% G-C content and one cut site by two enzymes, SalI and HindIII. The similar ori sequence of the pC194- rolling circle replication family (TTTATATTGAT) was located 63 bp upstream of the protein replication sequence, ORF 1. Total of five ORFs was identified and the coding sequence represented 4,966 nucleotides (70.4%). ORF1 of pILR091 had a low similarity with the sequence of pTE44. Other ORFs also showed low homology and E-values. The average G-C content of pILR091 was 39%, similar with that of genomic DNA. The copy number of pILR091 was determined at approximately 24 to 25 molecules per genomic DNA. These results suggested that pILR091 might be a good candidate to construct a new vector, which could be used for cloning and expression of foreign genes in lactobacilli.

Biodegradation of Abandoned Livestock by Blow Flies (Diptera: Calliphoridae) (검정파리(Calliphoridae)에 의한 폐가축의 분해 촉진)

  • Yun, Ji-Eun;Kang, Gi-Cheol;Park, Chung-Gyoo
    • Korean journal of applied entomology
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    • v.47 no.2
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    • pp.185-188
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    • 2008
  • A possible rapid biodegradation of abandoned livestock was tested by using the dead pig and chicken. The dead pig (36kg) was completely decomposed after 23 days of placement in the open field during June 2007. When the door of a cage in which a dead chicken (3.4kg) was placed was opened, a lot of flies were attracted to the chicken and layed eggs on the chicken. As the result the chicken was decomposed down to 0.6kg after 6 days of placement in the open field, On the other hand when the door was closed, the decomposition was very slow. The chicken weighed 3.0kg even after 6 days. An experiment conducted during October 2007 showed that decomposition speed depended on the number of inoculated flies. When 50 pairs of Lucilia sericata flies were inoculated to 3.2kg chicken, it was decomposed down to 1.0kg after 22 days in the field. However, when 200 female and 100 male flies were inoculated, the 3.4kg chicken was decomposed to 0.8kg after 11 days in the field. A 10,858 pupae (371.2g) was produced from the latter chicken. These pupae may possibly be used as a feed for fish and fowl. From these results it is considered that further research is needed to commercialize the blow flies for the rapid decomposition of an abandoned livestock of diverse size under diverse environment.

Investigation of blood biomarkers related to meat quality and quantity in Hanwoo steers

  • Moon, Yea Hwang;Cho, Woong Ki;Lee, Sung Sill
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.12
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    • pp.1923-1929
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    • 2018
  • Objective: This study was conducted to investigate the correlation between blood components and carcass traits, and to find the biomarkers related to meat quality and quantity in Hanwoo steers. Methods: One hundred twenty-six Hanwoo steers (8 to 9 months of age, body weight of $185.6{\pm}24.38kg$) were used to find the correlation between blood compositions and carcass traits. The steers were fed concentrate and rice straw (30 steers) or total mixed rations (96 steers) during the whole experimental period. Blood samples were collected from the jugular vein at the growing (8 to 12 months), fattening (13 to 23 months) and finishing phases (24 to 30 months). Steers were slaughtered at 30 to 31 months of age (body weight of $701.6{\pm}60.45kg$) and the carcass traits were evaluated. Blood metabolites and hormones were analyzed and the correlation coefficients and regression equations with carcass traits were determined. Results: Average concentrations of retinol, leptin and insulin were 1.10 IU, 30.34 ng, and 35.35 ng per mL of blood plasma, respectively. Retinol has negative correlations (p<0.01) with insulin and leptin. Blood insulin and total protein decreased with the age of steers, but retinol, aspartic acid transaminase (AST), glucose, cholesterol and triglyceride increased. In the finishing phase, significant (p<0.01) negative correlations occurred between blood retinol content and marbling score, and between blood AST content and longissimus muscle area of 13th rib, and the following regression equations were obtained: Marbling score $(1-9)=-0.009{\times}retinol(IU/100mL)+9.125$ ($R^2=0.643$), Longissimus muscle area $(cm^2)=-0.250{\times}AST(U/L)+112.498$ ($R^2=0.450$). Conclusion: It is possible to make highly marbled beef by controlling the blood retinol content during the fattening and finishing phases of Hanwoo steers. Accordingly, blood retinol and AST could be biomarkers for determining beef quality and quantity, respectively, prior to slaughter.

Factors Affecting the Security Ability of Port Logistics Organization Members (항만물류조직구성원들의 보안능력에 영향을 미치는 요인)

  • Kang, Da-Yeon
    • Journal of Navigation and Port Research
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    • v.43 no.3
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    • pp.179-185
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    • 2019
  • Currently, despite having active movements related to port logistics security, there is lack of awareness, education, and security systems related to port technology. Before implementing port logistics security, a mutual authentication agreement should be reached through the establishment of an integrated network that can share port logistics security information in real time. In order to achieve port competitiveness and strengthen logistics service, establishment of national strategy for logistics security is necessary. However, there is an urgent need to raise the security consciousness among the port logistics organization members and enhance the information security ability which is a crucial feature of the port logistics organization. Therefore, the objective of this study is to analyze the factors affecting the information security capacity of port logistics organization members. Even though the analysis rejected the hypothesis that security regulations affect security awareness, the security activities and security awareness were significantly correlated. It also has a positive impact on the relationship between security norms and security abilities, and security awareness and security abilities.

Influence of the Cure Systems on Long Time Thermal Aging Behaviors of NR Composites

  • Choi, Sung-Seen;Kim, Jong-Chul;Lee, Seung-Goo;Joo, Yong-L.
    • Macromolecular Research
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    • v.16 no.6
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    • pp.561-566
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    • 2008
  • NR composites with different curing systems were aged thermally at 60, 70, 80, and $90^{\circ}C$ for 2-185 days in a convection oven, and the changes in the crosslink density were investigated as a function of the accelerated thermal aging. The overall crosslink densities increased with increasing aging time irrespective of the aging temperatures and curing systems. The changes in crosslink density were enhanced by increasing the aging temperature. The degree of the increased crosslink density was in the following order: "the conventional cure system > the semi-EV system > the EV system". For short term thermal aging, the change in crosslink density with the aging time was complicated, particularly for low temperature aging. The activation energies of the change in crosslink density with thermal aging using the conventional and semi-EV cure systems increased and then remained relatively constant with increasing aging time, whereas that of the specimen with an EV cure system tended to increase linearly. The experimental results were explained by the dissociation of the existing polysulfidic linkages and the formation of new cross links through the crosslinking-related chemicals remaining in the sample.

Comparative Study on Public Health Facility Color Image Vocabulary among Countries -Focusing on korea and Romania- (공공보건시설 색채이미지에 대한 국가간 인식 비교 -한국과 루마니아 중심으로-)

  • Park, Heykyung;Adelean, Ioana;Kim, Hyeyeong;Oh, Jiyoung
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.3
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    • pp.185-191
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    • 2020
  • This study aims to understand the differences in cultural and emotional perceptions about the color image of public healthcare facilities in Romania, an Eastern European country that is relatively lacking in recognition but is gradually expanding trade. For this, color images were selected through a review of previous studies, and a questionnaire survey was constructed based on the colorimetric data by visiting 8 public healthcare facilities such as medical facilities, 4 social sports facilities, and 8 nursing facilities. An online survey was conducted on the color image of public facilities with 89 Koreans and 86 Romanians, and frequency and cross-analysis was conducted using the SPSS statistical analysis program to examine the color images of public healthcare facilities of Koreans and Romanians. The difference in perception was identified. As a result, it was found that there was a statistically significant difference in the perception of color images of public healthcare facilities between countries in vocabulary evaluation and image evaluation, and this was interpreted as different meanings for groups residing in different cultures. Therefore, it implies that cultural differences in perception should be considered when establishing an environment related to this in the future.

Self Charging Sulfanilic Acid Azocromotrop/Reduced Graphene Oxide Decorated Nickel Oxide/Iron Oxide Solar Supercapacitor for Energy Storage Application

  • Saha, Sanjit;Jana, Milan;Samanta, Pranab;Murmu, Naresh Chandra;Lee, Joong Hee;Kuila, Tapas
    • Composites Research
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    • v.29 no.4
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    • pp.179-185
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    • 2016
  • A self-charging supercapacitor is constructed through simple integration of the energy storage and photo exited materials at the photo electrode. The large band gap of $NiO/Fe_3O_4$ heterostructure generates photo electron at the photo electrode and store the charges through redox mechanism at the counter electrode. Sulfanilic acid azocromotrop/reduced graphene oxide layer at the photo electrode trapped the photo generated hole and store the charge by forming double layer. The solar supercapacitor device is charged within 400 s up to 0.5 V and exhibited a high specific capacitance of ~908 F/g against 1.5 A/g load. The solar illuminated supercapacitor shows a high energy and power density of 33.4 Wh/kg and 385 W/kg along with a very low relaxation time of ~15 ms ensuring the utility of the self charging device in the various field of energy storage and optoelectronic application.

Stabilization of Pb Contaminated Army Firing Range Soil using Calcined Waste Oyster Shells (소성가공 굴껍질을 이용한 군부대 사격장내 고농도 납 오염토양의 안정화)

  • Moon, Deok-Hyun;Cheong, Kyung-Hoon;Kim, Tae-Sung;Khim, Jee-Hyeong;Choi, Su-Bin;Ok, Yong-Sik;Moon, Ok-Ran
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.2
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    • pp.185-192
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    • 2010
  • The objective of this study was to investigate the effectiveness of stabilization for army firing range soil highly contaminated with Pb (total Pb: 29,000 mg/kg) using calcined waste oyster shells. The calcination was conducted to activate quicklime from calcite. In order to evaluate the effectiveness of calcination, both natural oyster shells (NOS) and calcined oyster shells (COS) were applied to the Pb contaminated soil. Stabilization was conducted by mixing the contaminated soil with oyster shell media at 5-20 wt% and cured for 28 days. Following 28 days of curing, Pb leachability was measured based on the Korean Standard Test method (0.1 N HCl extraction). The treatment results showed that the COS treatment outperformed the NOS treatment. All of the NOS treatments failed to meet the Korean warning standard of 100 mg/kg. However, the Pb concentrations were significantly reduced to 47 mg/kg and 3 mg/kg upon 15 wt% and 20 wt% COS treatments, respectively which passed the Korean warning standard. Moreover, -#20 mesh materials were more effective than the -#10 mesh materials in effectively reducing Pb leachability. The scanning electron microscopy (SEM)-energy dispersive X-ray spectroscopy (EDX) results indicated that Pb immobilization was strongly linked to Al and Si.

A Study on Human-Robot Interaction Trends Using BERTopic (BERTopic을 활용한 인간-로봇 상호작용 동향 연구)

  • Jeonghun Kim;Kee-Young Kwahk
    • Journal of Intelligence and Information Systems
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    • v.29 no.3
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    • pp.185-209
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    • 2023
  • With the advent of the 4th industrial revolution, various technologies have received much attention. Technologies related to the 4th industry include the Internet of Things (IoT), big data, artificial intelligence, virtual reality (VR), 3D printers, and robotics, and these technologies are often converged. In particular, the robotics field is combined with technologies such as big data, artificial intelligence, VR, and digital twins. Accordingly, much research using robotics is being conducted, which is applied to distribution, airports, hotels, restaurants, and transportation fields. In the given situation, research on human-robot interaction is attracting attention, but it has not yet reached the level of user satisfaction. However, research on robots capable of perfect communication is steadily being conducted, and it is expected that it will be able to replace human emotional labor. Therefore, it is necessary to discuss whether the current human-robot interaction technology can be applied to business. To this end, this study first examines the trend of human-robot interaction technology. Second, we compare LDA (Latent Dirichlet Allocation) topic modeling and BERTopic topic modeling methods. As a result, we found that the concept of human-robot interaction and basic interaction was discussed in the studies from 1992 to 2002. From 2003 to 2012, many studies on social expression were conducted, and studies related to judgment such as face detection and recognition were conducted. In the studies from 2013 to 2022, service topics such as elderly nursing, education, and autism treatment appeared, and research on social expression continued. However, it seems that it has not yet reached the level that can be applied to business. As a result of comparing LDA (Latent Dirichlet Allocation) topic modeling and the BERTopic topic modeling method, it was confirmed that BERTopic is a superior method to LDA.