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Analysis of nucleotide sequence of a novel plasmid, pILR091, from Lactobacillus reuteri L09 isolated from pig  

Lee, Deog-Yong (College of Veterinary Medicine, KRF Zoonotic Diseases Institute and BK 21 Program for Veterinary Science, Seoul National University)
Kang, Sang-Gyun (College of Veterinary Medicine, KRF Zoonotic Diseases Institute and BK 21 Program for Veterinary Science, Seoul National University)
Rayamajhi, Nabin (College of Veterinary Medicine, KRF Zoonotic Diseases Institute and BK 21 Program for Veterinary Science, Seoul National University)
Kang, Milan (College of Veterinary Medicine, KRF Zoonotic Diseases Institute and BK 21 Program for Veterinary Science, Seoul National University)
Yoo, Han Sang (College of Veterinary Medicine, KRF Zoonotic Diseases Institute and BK 21 Program for Veterinary Science, Seoul National University)
Publication Information
Korean Journal of Veterinary Research / v.48, no.4, 2008 , pp. 441-449 More about this Journal
Abstract
The genus Lactobacillus is the largest of the genera included in lactic acid bacteria and is associated with mucosal membranes of human and animal. Only a few Lactobacillus plasmid-encoded functions have been discovered and used. In this study, a novel plasmid (pILR091) was isolated from a wild L. reuteri isolated from pig and described the characteristics of its replicons, genetic organization, and relationship with other plasmids. After digestion of the plasmid, pILR091, with SalI, plasmid DNA was cloned into the pQE-30Xa vector and sequenced. The complete sequence was confirmed by the sequencing of PCR products and analyzed with the Genbank database. The isolate copy number and stability were determined by quantitative-PCR. The complete sequence of L. reuteri contained 7,185 nucleotides with 39% G-C content and one cut site by two enzymes, SalI and HindIII. The similar ori sequence of the pC194- rolling circle replication family (TTTATATTGAT) was located 63 bp upstream of the protein replication sequence, ORF 1. Total of five ORFs was identified and the coding sequence represented 4,966 nucleotides (70.4%). ORF1 of pILR091 had a low similarity with the sequence of pTE44. Other ORFs also showed low homology and E-values. The average G-C content of pILR091 was 39%, similar with that of genomic DNA. The copy number of pILR091 was determined at approximately 24 to 25 molecules per genomic DNA. These results suggested that pILR091 might be a good candidate to construct a new vector, which could be used for cloning and expression of foreign genes in lactobacilli.
Keywords
G-C content; Lactobacillus reuteri; ORF; plasmid DNA; replication;
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1 Benachour A, Frere J, Novel G. pUCL287 plasmid from Tetragenococcus halophila (Pediococcus halophilus) ATCC 33315 represents a new theta-type replicon family of lactic acid bacteria. FEMS Microbiol Lett 1995, 128, 167-175   DOI
2 El-Osta YG, Hillier AJ, Dobos M. Construction of a combined physical and genetic map of the chromosome of Lactobacillus acidophilus ATCC 4356 and characterization of the rRNA operons. Microbiology 2005, 151, 875-892   DOI   ScienceOn
3 Gfeller KY, Roth M, Meile L, Teuber M. Sequence and genetic organization of the 19.3-kb erythromycinand dalfopristin-resistance plasmid pLME300 from Lactobacillus fermentum ROT1. Plasmid 2003, 50, 190-201   DOI   ScienceOn
4 Giulietti A, Overbergh L, Valckx D, Decallonne B, Bouillon R, Mathieu C. An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods 2001, 25, 386-401   DOI   ScienceOn
5 Kiewiet R, Kok J, Seegers JF, Venema G, Bron S. The Mode of Replication Is a Major Factor in Segregational Plasmid Instability in Lactococcus lactis. Appl Environ Microbiol 1993, 59, 358-364
6 Lortal S, Rouault A, Guezenec S, Gautier M. Lactobacillus helveticus: strain typing and genome size estimation by pulsed field gel electrophoresis. Curr Microbiol 1997, 34, 180-185   DOI
7 Mayo B, Hardisson C, Brana AF. Selected characteristics of several strains of Lactobacillus plantarum. Microbiologia 1989, 5, 105-112
8 Perez-Arellano I, Zuniga M, Perez-Martinez G. Construction of compatible wide-host-range shuttle vectors for lactic acid bacteria and Escherichia coli. Plasmid 2001, 46, 106-116   DOI   ScienceOn
9 Daeschel MD, Andersson RE, Fleming HP. Microbial ecology of fermenting plant materials. FEMS Microbiol Rev 1987, 46, 357-367   DOI
10 Abs El-Osta YG, Hillier AJ, Davidson BE, Dobos M. Pulsed-field gel electrophoretic analysis of the genome of Lactobacillus gasseri ATCC33323, and construction of a physical map. Electrophoresis 2002, 23, 3321-3331   DOI   ScienceOn
11 Trautwetter A, Ritzenthaler P, Alatossava T, Mata-Gilsinger M. Physical and genetic characterization of the genome of Lactobacillus lactis bacteriophage LLH. J Virol 1986, 59, 551-555
12 Skaugen M. The complete nucleotide sequence of a small cryptic plasmid from Lactobacillus plantarum. Plasmid 1989, 22, 175-179   DOI   ScienceOn
13 van Kranenburg R, Golic N, Bongers R, Leer RJ, de Vos WM, Siezen RJ, Kleerebezem M. Functional analysis of three plasmids from Lactobacillus plantarum. Appl Environ Microbiol 2005, 71, 1223-1230   DOI   ScienceOn
14 Anderson DG, McKay LL. In vivo cloning of lac genes in Streptococcus lactis ML3. Appl Environ Microbiol 1984, 47, 245-249
15 Klein JR, Ulrich C, Plapp R. Characterization and sequence analysis of a small cryptic plasmid from Lactobacillus curvatus LTH683 and its use for construction of new Lactobacillus cloning vectors. Plasmid 1993, 30, 14-29   DOI   ScienceOn
16 Schleifer KH, Stackebrandt E. Molecular systematics of prokaryotes. Annu Rev Microbiol 1983, 37, 143-187   DOI   ScienceOn
17 Park WJ, Lee KH, Lee JM, Lee HJ, Kim JH, Lee JH, Chang HC, Chung DK. Characterization of pC7 from Lactobacillus paraplantarum C7 derived from Kimchi and development of lactic acid bacteria-Escherichia coli shuttle vector. Plasmid 2004, 52, 84-88   DOI   ScienceOn
18 Wang TT, Lee BH. Plasmids in Lactobacillus. Crit Rev Biotechnol 1997, 17, 227-272   DOI
19 Summers DK. The Biology of Plasmids. pp. 31-64, 92-110, Blackwell Science, Oxford, 1996
20 Chassy BM, Gibson E, Giuffrida A. Evidence for extrachromosomal elements in Lactobacillus. J Bacteriol 1976, 127, 1576-1578
21 Heng NC, Bateup JM, Loach DM, Wu X, Jenkinson HF, Morrison M, Tannock GW. Influence of different functional elements of plasmid pGT232 on maintenance of recombinant plasmids in Lactobacillus reuteri populations in vitro and in vivo. Appl Environ Microbiol 1999, 65, 5378-5385
22 Gruss A, Ehrlich SD. The family of highly interrelated single-stranded deoxyribonucleic acid plasmids. Microbiol Rev 1989, 53, 231-241
23 Primrose SB, Twyman RM, Old RW. Principles of Gene Manipulation. 6th ed. pp. 43-63, Blackwell Science, Oxford, 2001
24 Sharpe ME. The genus Lactobacillus. In: Starr MP, Stolp H, Truper HG, Balows A, Schlegel HG (eds.). The Prokaryotes. A Handbook on Habitant, Isolation, and Identification of Bacteria. pp.1653-1674, Springer-Verlag, Berlin, 1981
25 Salminen S, Wright AV, Ouwehand A. Lactic Acid Bacteria: Microbiological and Functional Aspects. 3rd ed. pp. 249-293, Marcel Dekker, NewYork, 2004
26 O'Sullivan DJ, Klaenhammer TR. Rapid Mini-Prep Isolation of High-Quality Plasmid DNA from Lactococcus and Lactobacillus spp. Appl Environ Microbiol 1993, 59, 2730-2733
27 Ruiz-Barba JL, Piard JC, Jimenez-Diaz R. Plasmid profiles and curing of plasmids in Lactobacillus plantarum strains isolated from green olive fermentations. J Appl Bacteriol 1991, 71, 417-421   DOI
28 Alpert CA, Crutz-Le Coq AM, Malleret C, Zagorec M. Characterization of a theta-type plasmid from Lactobacillus sakei: a potential basis for low-copynumber vectors in lactobacilli. Appl Environ Microbiol 2003, 69, 5574-5584   DOI
29 Haddadin MSY, Awaisheh SS, Robinson RK. The production of yoghurt with probiotic bacteria isolated from infants in Jordan. Pak J Nutr 2004, 3, 290-293   DOI
30 Kandler O, Weiss D. Regular, non-sporing grampositive rods. In: Sneath PHA, Mair NS, Sharpe ME, Holt JG (eds.). Bergey's Manual of Systematic Bacteriology. Vol. 2. pp. 1208-1234, Williams and Wilkins, Baltimore, 1986
31 Nicoloff H, Bringel F. ISLpl1 is a functional IS30-related insertion element in Lactobacillus plantarum that is also found in other lactic acid bacteria. Appl Environ Microbiol 2003, 69, 6032-6040   DOI
32 Desmond C, Ross RP, Fitzgerald G, Stanton C. Sequence analysis of the plasmid genome of the probiotic strain Lactobacillus paracasei NFBC338 which includes the plasmids pCD01 and pCD02. Plasmid 2005, 54, 160-175   DOI   ScienceOn
33 Salminen S, Wright AV, Ouwehand A. Lactic Acid Bacteria: Microbiological and Functional Aspects. 2nd ed. pp. 475-518, Marcel Dekker, ,NewYork, 1998
34 Sorvig E, Skaugen M, Naterstad K, Eijsink VG, Axelsson L. Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin-antitoxin-like plasmid maintenance system. Microbiology 2005, 151, 421-431   DOI   ScienceOn
35 Kiewiet R, Bron S, de Jonge K, Venema G, Seegers JFML. Theta replication of the lactococcal plasmid pWVO2. Mol Microbiol 1993, 10, 319-327   DOI   ScienceOn
36 Pavlova SI, Kilic AO, Topisirovic L, Miladinov N, Hatzos C, Tao L. Characterization of a cryptic plasmid from Lactobacillus fermentum KC5b and its use for constructing a stable Lactobacillus cloning vector. Plasmid 2002, 47, 182-192   DOI   ScienceOn
37 Kullen MJ, Klaenhammer. Genetic modification of intestinal lactobacilli and bifidobacteria. Curr Issues Mol Biol 2000, 2, 41-50
38 Gardner MN, Deane SM, Rawlings DE. Isolation of a new broad-host-range IncQ-like plasmid, pTC-F14, from the acidophilic bacterium Acidithiobacillus caldus and analysis of the plasmid replicon. J Bacteriol 2001, 183, 3303-3309   DOI   ScienceOn
39 Kok J, van der Vossen JM, Venema G. Construction of plasmid cloning vectors for lactic streptococci which also replicate in Bacillus subtilis and Escherichia coli. Appl Environ Microbiol 1984, 48, 726-731