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The Variation of Nutural Population of Pinus densiflora S. et Z. in Korea -Change of variance due to number of family as sample size to affirm the population and family variations- (소나무 천연집단(天然集團)의 변이(變異)에 관(關)한 연구(硏究)(IV) -집단간(集團間) 및 가계간분산(家系間分散) 추정(推定)에 영향하는 가계수(家系數)에 대하여-)

  • Yim, Kyong Bin;Kwon, Ki Won;Lee, Kyong Jae
    • Journal of Korean Society of Forest Science
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    • v.35 no.1
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    • pp.39-46
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    • 1977
  • In the analysis of variance between population and between individual trees (families), the fluctuation of values of variances due to sample size, (number of family) was analysed by two different designs, i.e. 2-level nested design with equal sample size and randomized complete block design. The variables were seedling heights and root calipers of 1-0 and 1-1 seedlings of Pinus densiflora S. et Z. The details of three natural stands and their progeny characters were presented in previous reports. 1. In nested design analysis. increase of sample size resulted the decrease of F-values among families in general, however, the F-values among populations showen the increasing tendency. The smaller the sample size, the larger the F-values fluctuation was resulted in general. At the point of beyond sample size 10, however, the fluctuation become to be stabilized. The F-value fluctuation seemed to be more in the case of analysis with random sampling method than with sequentially accumulated sampling method. And also such a tendency was more obvious in smaller sample size than in large one. 2. In R.C.B.D. analysis, the sample size to affirm the family variation was smaller than that for population variations.

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Necessity of Education for Emergency Medical Technicians to Improve Awareness of Early Symptoms of Stroke and Assessment of Stroke Patients (응급구급대원에서 뇌졸중 조기증상 인지 및 환자평가 향상을 위한 교육 필요성)

  • Lee, Jeong-Mi;Lee, Jang-Yeol;Park, Seong-Bin;Lee, Young-Hoon;Oh, Gyung-Jae
    • Journal of agricultural medicine and community health
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    • v.38 no.2
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    • pp.130-141
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    • 2013
  • Objectives: The purpose of this study was to reconsider the necessity of stroke-related educational programs by investigating early symptoms of stroke that emergency medical technicians (EMTs) may experience. Methods: An interview survey was carried out, targeting all EMTs 298 people who were working at 119 fire safety centers and local units in Jeollabuk-do province, from April 1 to 30, 2011. An interviewer administered questionnaire was used to obtain data on ETMs' demographic characteristics, educational level on stroke, and awareness level about early symptoms of stroke and treatment-related characteristics of stroke patients. Results: The results showed that 63.2% of those surveyed had completed the required educational programs on stroke during the past year. The level of awareness about early symptoms of stroke and assessment of stroke patients' condition was relatively high for women, those who were 30 years under, low position people, emergency service practitioners, emergency medical technicians, and people who have completed education, respectively. It was also turned out that 82.8% of those surveyed took stroke patients to the nearest hospitals first so that they could receive appropriate treatment. The level of awareness about early symptoms of stroke and assessment of stroke patients' condition in people who have completed education were higher than non-complete. The level of awareness about early symptoms of stroke was positively correlated with confidence, satisfaction and appropriateness in treatment of stroke patients. Conclusions: These results strongly suggest that it is necessary to operate specialized educational programs to enhance EMTs' appropriate awareness of the early symptoms of stroke and assessment of stroke patient's condition.

Survey on the Pesticides Suspected as an Endocrine Disrupter In Agricultural Products Distributed in Seoul (서울지역 유통 농산물 중 내분비계 장애 추정농약의 잔류실태 조사)

  • Kim, Ouk-Hee;Park, Sung-Kyu;Choi, Young-Hee;Seoung, Hyun-Jung;Han, Sung-Hee;Lee, Young-Ju;Jang, Jung-Im;Kim, Yun-Hee;Jo, Han-Bin;Park, Geon-Yong;Yu, In-Sil;Han, Ki-Young
    • The Korean Journal of Pesticide Science
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    • v.15 no.1
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    • pp.36-47
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    • 2011
  • This study was performed to investigate the contaminated status of endocrine disruptor-suspected pesticides in agricultural products distributed in northern Seoul in 20l0. We analyzed 33 kinds of pesticides by multiresidue analysis method in 3,081 samples. Twelve pesticidcs were detected 600 times and violated 22 times from the agricultural products. The highest frequency of detection was procymidone and that of violation was endosulfan. The highest rates of detection divided violation times as diazinon. In agricultural products, 466 samples had pesticide residues and 22 samples violated the maximum residue limits (MRLs). Agricultural products were also classified by type and red pepper powder belonged to the others was the highest detection rate at 70.7%, fruiting vegetables 35.3%, tea leaves 23.6%, stalk and stem vegetables 21.2%, fruits 14.6% and leafy vegetables 11.2%. The rates of violation sample were stalk and stem vegetables 1.1%, leafy vegetables 1.0% and roots and tubers 0.4%. Leek was to be managed primarily because it had the high detection and violation ratio and was detected together several pesticides.

$^{99m}Tc$-HMPAO-labelled Leucocyte Scintigraphy in the Diagnosis of Infection after Total Knee Replacement Arthroplasty (인공슬관절 전치환술 환자에서 $^{99m}Tc$-HMPAO-백혈구 스캔을 이용한 인공관절 감염의 진단)

  • Park, Dong-Rib;Kim, Jae-Seung;Ryu, Jin-Sook;Moon, Dae-Hyuk;Bin, Seong-Il;Cho, Woo-Shin;Lee, Hee-Kyung
    • The Korean Journal of Nuclear Medicine
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    • v.33 no.4
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    • pp.413-421
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    • 1999
  • Purpose: This study was performed to evaluate the usefulness of $^{99m}Tc$-HMPAO-labelled leucocyte scintigraphy for diagnosing prosthetic infection after total knee replacement arthroplasty without the aid of following bone marrow scintigraphy Materials and Methods: The study subjects were 25 prostheses of 17 patients (one man and 16 women, mean age. 65 years) who had total knee replacement arthroplasty. After injection of $^{99m}Tc$-HMPAO-labelled leucocyte, the whole body planar and knee SPECT images were obtained in all patients. The subjects were classified into three groups according to clinical suspicion of prosthetic infection. Group A (n=11) with high suspicion of infection; Group B (n=6) with equivocal suspicion of infection, and Group C (n=8) with asymptomatic contralateral prostheses. Final diagnosis of infection was based on surgical, histological and bacteriological data and clinical follow-up. Results: Infection was confirmed in 13 prostheses (11 in Group A and 2 in Group B). All prostheses in Group A were true positive. There were two true positives, one false positive and three true negatives in Group B, and six true negatives and two false positives in Group C. Overall sensitivity, specificity, and accuracy for diagnosis of the infected knee prosthesis were 100%, 75% and 88%, respectively Conclusion: $^{99m}Tc$-HMPAO-labelled leucocyte scintigraphy is a sensitive method for the diagnosis of infected knee prosthesis. However, false positive uptakes even in asymptomatic prosthesis suggest that bone marrow scintigraphy may be needed to achieve improved specificity.

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Food Habits of the Glass eel Anguilla japonica in the West Coast Estuaries of Korean Peninsula Determined by Using C and N Stable Isotopes (안정동위원소를 이용한 서해연안 실뱀장어의 먹이 습성)

  • Kim, Jeong Bae;Lee, Won-Chan;Kim, Dae-Jung;Seong, Ki Baik;Choi, Hee-Gu;Choi, Woo-Jeung;Hwang, Hak Bin;Hong, Sokjin;Kim, Hyung Chul;Park, Sung-Eun;Shim, Jeong Hee;Kang, Chang-Keun
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.18 no.4
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    • pp.206-213
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    • 2013
  • Glass eels (Anguilla japonica) are caught in the west coast of Korea on their migratory route from the breeding grounds in the Mariana Trench along the North Equatorial Current and the Kuroshio Current. To identify the food source of natural glass eels, we analyzed the stable C and N isotopes of glass eels caught in April 2012 and investigated possible food sources in the survey area. In particular, with respect to the stable C and N isotopes of particulate organic matter, we extended the surveying area to the northern parts of East China Sea as well as the west coast of Korea. The stable C and N isotope ratios of the glass eels caught in the west coast were found to be $-20.7{\pm}0.1$‰ and $5.0{\pm}0.2$‰, respectively. The stable C and N isotope ratios of the particulate organic matter in the west coast of Korea, in which the glass eels are assumed to eat the particulate organic matter as food source, were estimated to be $-24.0{\pm}0.3$‰ and $2.8{\pm}0.4$‰, respectively. Similar data were obtained from the northern part of the East China Sea, $-24.5{\pm}0.5$‰ and $0.8{\pm}0.3$‰. The stable isotope ratios showed values differing from the stepwise increasing rates up the food web in natural aquatic ecosystem, showing that particulate organic matter in the west coast of Korea and East China Sea was not served as the glass eels food source. This result suggested that the glass eels caught in the west coast might not assimilate nutrition from the marine environment during long migration.

Development of a Simultaneous Analysis Method for DDT (DDD & DDE) in Ginseng (인삼 중 DDT(DDD 및 DDE) 분석법의 개발)

  • Kim, Sung-Dan;Cho, Tae-Hee;Han, Eun-Jung;Park, Seoung-Gyu;Han, Chang-Ho;Jo, Han-Bin;Choi, Byung-Hyun
    • Korean Journal of Food Science and Technology
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    • v.40 no.2
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    • pp.123-128
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    • 2008
  • The MRLs (maximum residue limits) of DDT (DDD and DDE) in fresh ginseng, dried ginseng, and steamed red ginseng are set as low as 0.01 mg/kg, 0.05 mg/kg, and 0.05 mg/kg, respectively. Therefore, this study was undertaken to develop a simple and highly sensitive analysis method, as well as to reduce interfering ginseng matrix peaks, for the determination of DDT isomers (o,p'-DDE, p,p'-DDE, o,p'-DDD, p,p'-DDD, o,p'-DDT, and p,p'-DDT) in fresh ginseng, dried ginseng, and steamed red ginseng at the 0.01 mg/kg level. The method used acetonitrile extraction according to simultaneous analysis, followed by normal-phase Florisil solid-phase extraction column clean-up. The purification method entailed the following steps: (1) dissolve the concentrated sample extract in 7 mL hexane; (2) add 3 mL of $H_2SO_4$; (3) vigorously shake on avortex mixer; (4) cetrifuge at 2000 rpm for 5 min; (5) transfer 3.5 mL of the supernatant to the Florisil-SPE (500 mg/6 mL);and (6) elute the SPE column with 1.5 mL of hexane and 10 mL of ether/hexane (6:94). The determination of DDT isomers was carried out by a gas chromatography-electron capture detector (GC-${\mu}$ECD). The hexane and ether/hexane (6:94) eluate significantly removed chromatographic interferences, and the addition of 30% $H_2SO_4$ to the acetonitrile extract effectively reduced many interfering ginseng matrix peaks, to allow for the determination of the DDT isomers at the 0.01 mg/kg level. The recoveries of the 6 fortified (most at 0.01 mg/kg) DDT isomers from fresh ginseng, dried ginseng, and steamed red ginseng ranged from 87.9 to 99.6%. The MDLs (method detection limits) ranged from 0.003 to 0.009 mg/kg. Finally, the application of this method for the determination of DDT isomers is sensitive, rapid, simple, and inexpensive.

Antioxidative Effects and Tyrosinase Inhibitory Activities of Mate (Ilex paraguariensis) Extract/Fractions (마테(Ilex paraguariensis) 추출물/분획물의 항산화능 및 타이로시네이즈 저해 활성)

  • Kong, Bong Ju;Kim, Yong Jae;Baek, Jee Seon;Lee, Da Bin;Lee, Ji Won;Min, Na Young;Kim, A Young;Park, Soo Nam
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.4
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    • pp.391-400
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    • 2015
  • In the present study, 50% ethanol extract, the ethyl acetate and aglycone fraction were prepared from mate (Ilex paraguariensis) and their antioxidative ability was evaluated. The yields of extract and fractions were 32.0, 4.48 and 0.82% per dried powder, respectively. Free radical scavenging activities were performed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and total antioxidant capacity was estimated using luminol-dependent chemiluminescence assay. Free radical scavenging activities ($FSC_{50}$) of 50 % ethanol extract, ethyl acetate fraction and aglycone fraction were 8.83, 5.84 and $6.05{\mu}g/mL$, respectively. Their total antioxidant capacities ($OSC_{50}$) were similar to that of L-ascorbic acid ($1.72{\mu}g/mL$), known as a prominent water soluble antioxidant, in all extracts and 50% ethanol extract ($1.03{\mu}g/mL$) was the most effective. The cellular protective effects on the $^1O_2$-induced cellular damage of erythrocytes were evaluated and the results showed that all extracts were significantly higher than (+)-${\alpha}$-tocopherol at $10{\mu}g/mL$. Especially, the ${\tau}_{50}$ value of aglycone fraction was 5 times higher than (+)-${\alpha}$-tocopherol at $10{\mu}g/mL$ and $50{\mu}g/mL$. The inhibitory effects of the ethyl acetate and aglycone fractions on tyrosinase were similar to arbutin, known as the whitening agent in cosmetics. These results suggest that the extracts of mate have the applicability as antioxidant and anti-aging cosmeceutical ingredients.

Effects of Supplementation of Multienzymes in Diets Containing Different Energy Levels on Growth Performance, Nutrient Digestibility, Blood Metabolites, Microbiota and Intestinal Morphology of Broilers (에너지 수준이 다른 사료에 복합효소제의 첨가가 육계의 사양성적, 영양소 소화율, 혈액성상, 장내미생물 균총 및 소장 융모에 미치는 영향)

  • Shim, Young Ho;Kim, Jin Soo;Hosseindoust, Abdolreza;Ingale, Santosh Laxman;Choi, Yo Han;Kim, Min Ju;Ohh, Seung Min;Ham, Hyung Bin;Chae, Byung Jo
    • ANNALS OF ANIMAL RESOURCE SCIENCES
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    • v.28 no.3
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    • pp.97-107
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    • 2017
  • The present study was conducted to investigate the effects on growth performance, nutrient digestibility, and gut health of broiler chickens when a dietary supplementation of multienzymes was added to diets, containing different energy levels. A total of 480 broiler chickens of similar body weight (Ross 308, 1-day-old) were randomly subjected to four treatments. The dietary treatments included a corn-soybean meal-based diet supplemented with: multienzyme (amylase+protease+ mannanase+xylanase+phytase), 0.05% enzyme, and different energy levels (3010 and 3060 kcal/kg). The experimental diets were fed to the chicks in a mash form for 35 days in two phases (1-21 d, phase I; and 22-35 d, phase II). During the overall period, chicks fed with diets supplemented with multienzymes had a better weight gain (p<0.05) and feed conversion ratio (FCR) than those fed with diets without enzymes. There was no difference in the growth rate and FCR among the chicks fed with diets supplemented with enzymes, even though the dietary energy levels were different. The apparent fecal and ileal digestibility of dry matter, gross, crude protein, calcium, and phosphorus were significantly enhanced (p<0.05). The population of cecal and ileal Lactobacillus spp. was significantly increased (p<0.05), and Clostridium spp. and coliforms were significantly decreased (p<0.05) in diets supplemented with enzymes. Villus height and villus height to crypt depth ratio in the small intestine was also significantly enhanced (p<0.05) in diets supplemented with enzymes. In conclusion, multienzyme supplementation had positive effects on the weight gain of broilers, FCR, digestibility of nutrients, and on the growth of intestinal microbiota.

Effect of Holding Solution on Vase Life of a New Ornamental Crop Known as Euphorbia jolkinii Boiss. (보존용액 처리가 신 관상식물 암대극(Euphorbia jolkinii Boiss.)의 절화수명에 미치는 영향)

  • Song, Su Jung;Park, Hyung Bin;Kim, Ji Sun;Oh, Hye Jin;Kim, Sang Yong;Jeong, Mi Jin;Lee, Seung Youn
    • Korean Journal of Plant Resources
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    • v.32 no.4
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    • pp.312-317
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    • 2019
  • This study was conducted with the purpose of examining the suitability of Euphorbia jolkinii Boiss. as cut flower, so that it may be introduced as a new ornamental crop. For this purpose, effect of various holding solutions on vase solution uptake rate, vase life, and relative fresh weight of cut flowering branches of E. jolkinii was examined. After harvest, cut branches were treated with 10, 50, and $100mg{\cdot}L^{-1}$ of 8-hydroxyquinoline sulfate (8-HQS), 0.1 and 0.2 mM of silver thiosulfate (STS), Chrysal, and Floralife. The cut branches of E. jolkinii were placed under the environmental conditions maintained at air temperature of $22.6^{\circ}C$, relative humidity of 45%, and 9/15h photoperiod that was controlled using fluorescent lamps (light intensity of $9.89{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). A holding solution containing $10mg{\cdot}L^{-1}$ 8-HQS was found to be significantly effective for vase solution uptake rate compared to control, $50mg{\cdot}L^{-1}$ 8-HQS, and $100mg{\cdot}L^{-1}$ 8-HQS treatments. However, no significant difference was found in vase life between the branches treated with $10mg{\cdot}L^{-1}$ 8-HQS holding solution and branches of the control group. Increasing holding solution concentrations of STS was found to have negative effect on the vase life of cut E. jolkinii branches. Relative fresh weight of cut E. jolkinii branches were significantly decreased by two commercial holding solutions, Chrysal and Floralife. It is expected that these results would aid further studies on utilization of E. jolkinii as cut flower crop.

Effect of Final Irrigation Timing before Simulated Dark Shipping on Post-shipping Performance of Potted Phalaenopsis Sogo Yukidian 'V3' (팔레놉시스 분화의 모의수송 전 최종 관수 시기가 수송 후 생육에 미치는 영향)

  • Jeong, Ju Hui;Jeon, Jeong Bin;Kim, Sang Yoon;Oh, Wook
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.65-71
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    • 2021
  • This study was carried out to investigate the effect of the final irrigation timing (FIT) before packaging for long-term transportation on growth, flowering, and crop quality of Phalaenopsis after simulated dark shipping (SDS). Phalaenopsis Sogo Yukidian 'V3' plants grown in 11 cm-diameter plastic pots filled with potting media (sphagnum moss + bark or only sphagnum moss) were packaged in paper boxes for export at 3.5, 7, 10 days (FIT 3.5, 7,10; Experiment 1) and 4, 6, 8, 10 days (FIT 4, 6, 8, 10; Experiment 2) after the final irrigation and then stored in a growth chamber at 20 ± 1℃ and 70 ± 3% RH created for SDS. After 4 weeks, the plants were taken out and grown in a greenhouse at 23 ± 3℃ and 70 ± 5% RH, and crop characteristics were measured during cultivation. In Experiment 1, the survival rate of FIT 3.5 plants was lower than that of FIT 7 and FIT 10. There was no difference between treatments in days to first flower, the number of florets, and the elongation rate of flower stalks. In Experiment 2, the percentage of rotted leaves was lowest in FIT 6 when before forcing and at 12 weeks after forcing, and that of FIT 8 was similar to FIT 6 when before forcing, but slightly increased after 12 weeks. The percentage of rotted leaves of FIT 10 was highest and that of FIT 4 was also high. There was little difference in flowering characteristics among treatments. In conclusion, the FIT before packaging for long-term (4 weeks) transportation of potted Phalaenopsis 'V3' affected the leaf rot rather than the post-shipping growth and flowering. And it was considered appropriate to set the volumetric water content of the potting media just before packaging to about 30%.