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Preparation and BDNF Release Profile of BDNF-loaded PLGA Scaffolds for Tissue Engineered Nerve Regeneration (신경재생을 위한 BDNF를 함유한 PLGA 지지체의 제조 및 방출)

  • Kim, Cho-Min;Kim, Soon-Hee;Oh, A-Young;Kim, Geun-Ah;Lee, Il-Woo;Rhee, John-M.;Khang, Gil-Son
    • Polymer(Korea)
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    • v.32 no.6
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    • pp.529-536
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    • 2008
  • We manufactured poly (L-lactide-co-glycolide) (PLGA) scaffolds impregnated demineralized bone particle (DBP) and hyaluronic acid (HA) by ice-particle leaching method and tested their ability of sustained release of brain derived neurotrophic factor (BDNF). BDNF (50 and 200 ng) mixed with PLGA, DBP/PLGA, HA/PLGA and DBP/HA/PLGA scaffold. The release profiles of BDNF from BDNF loaded scaffolds were assayed using ELISA. Morphological changes of scaffolds by BDNF release were also observed by SEM. BDNF stably and sustainedly released from DBP/HNPLGA than from PLGA and DBP/PLGA scaffolds. DBP/HA/PLGA scaffolds showed the great structural changes, which demonstrated BDNF release amount from DBP/HA/PLGA scaffolds were highest in all groups. We suggest that BDNF loaded DBP/HNPLGA scaffold would be very useful for nerve regeneration.

Adhesion and Proliferation Behavior of Retinal Pigment Epithelial Cells on Hesperidin/PLGA Films (헤스페리딘/PLGA 필름에서 망막색소상피세포의 부착과 증식거동)

  • Lee, So Jin;Kang, Su Ji;Kim, Hye Yun;Lee, Jung Hwan;Kim, Eun Young;Kwon, Soon Yong;Chung, Jin Wha;Joo, Choun-Ki;Khang, Gilson
    • Polymer(Korea)
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    • v.38 no.1
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    • pp.24-30
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    • 2014
  • Retinal pigment epithelium (RPE) plays an important role in maintaining the visual function and the degeneration of the RPE causes several retinal degeneration disease. In order to fabricate the suitable carrier for RPE transplantation, the hybrid poly(lactide-co-glycolide) (PLGA) film with hesperidin was prepared. Hesperidin has an anti-inflammatory and antioxidant characteristics. ARPE-19 was seeded on hesperidin/PLGA film and then, cell proliferation was determined by the MTT assay, and cell adhesion and cell morphology were confirmed by SEM. Also, RT-PCR was performed to confirm the expression of the specific genes, and AEC immunohistochemical staining was performed to determine the expression of RPE65. As a result, we confirmed that attachment, proliferation and phenotype maintenance of RPE cells were more excellent on hesperidin/PLGA film than PLGA film, thereby we were able to confirm the potential applications of hesperidin/PLGA film as tissue engineering carrier for regeneration of retina.

SRM Speed Control using Sliding Mode Control (Sliding Mode Control 기법을 이용한 SRM 속도 제어)

  • Im, Jong-Bin;Lim, Seung-Bin;Lee, Sung-Gu;Go, Sung-Chul;Lee, Ju
    • Proceedings of the KIEE Conference
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    • 2006.04b
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    • pp.176-178
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    • 2006
  • This paper is intended to research the speed response of switched reluctance motor (SRM) using sliding mode control method when load is changing. The simulation compare only control by PI controller with control by PI controller and sliding mode controller. It compare with speed response using MATLAB/SIMULINK.

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The Development of Multi-view point Image Interpolation Method Using Real-image

  • Yang, Kwang-Won;Park, Young-Bin;Huh, Kyung-Bin
    • 제어로봇시스템학회:학술대회논문집
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    • 2001.10a
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    • pp.129.1-129
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    • 2001
  • In this paper, we present an approach for matching images from finding interesting points and applying new image interpolation algorithm. New algorithms are developed that automatically align the input images match them and reconstruct 3-D surfaces. The interpolation algorithm is designed to cope with simple shapes. The proposed image interpolation algorithm generate a rotation image about vertical axes by an any angle from 4 base images. Each base image that was obtained from CCD camera has an angle difference of 90$^{\circ}$ The proposed image interpolation algorithm use the geometric analysis of image and depth information.

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Prediction of Temperature, Moisture Content and Quality Changes in Stored Bulk Rough Rice (시뮬레이션에 의한 산물(散物) 저장(貯藏) 벼의 온도(溫度), 함수율(含水率) 및 품질변화(品質變化)의 예측(豫測))

  • Keum, D.H.;Kim, J.Y.
    • Journal of Biosystems Engineering
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    • v.16 no.1
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    • pp.49-59
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    • 1991
  • A numerical model was developed to predict grain temperature, moisture content, and drymatter loss of rough rice in a grain storage bin. This model simulated conduction, natural convection, and mass transfer occuring inside a storage bin. The results obtained from the study were as follows. 1. The predicticted results agreed well with the measured results. 2. Rough rice could be store safely for one year in Suweon, Dajeon, and Jingu area. 3. Aeration of 5-day was required to control grain temperature and moisture content rise early in Jun and July, respectively.

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