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Preparation and BDNF Release Profile of BDNF-loaded PLGA Scaffolds for Tissue Engineered Nerve Regeneration  

Kim, Cho-Min (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Kim, Soon-Hee (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Oh, A-Young (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Kim, Geun-Ah (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Lee, Il-Woo (Department of Neurosurgery, College of Medicine, The Catholic University of Korea)
Rhee, John-M. (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Khang, Gil-Son (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Publication Information
Polymer(Korea) / v.32, no.6, 2008 , pp. 529-536 More about this Journal
Abstract
We manufactured poly (L-lactide-co-glycolide) (PLGA) scaffolds impregnated demineralized bone particle (DBP) and hyaluronic acid (HA) by ice-particle leaching method and tested their ability of sustained release of brain derived neurotrophic factor (BDNF). BDNF (50 and 200 ng) mixed with PLGA, DBP/PLGA, HA/PLGA and DBP/HA/PLGA scaffold. The release profiles of BDNF from BDNF loaded scaffolds were assayed using ELISA. Morphological changes of scaffolds by BDNF release were also observed by SEM. BDNF stably and sustainedly released from DBP/HNPLGA than from PLGA and DBP/PLGA scaffolds. DBP/HA/PLGA scaffolds showed the great structural changes, which demonstrated BDNF release amount from DBP/HA/PLGA scaffolds were highest in all groups. We suggest that BDNF loaded DBP/HNPLGA scaffold would be very useful for nerve regeneration.
Keywords
brain derived neurotrophic factor; poly(L-lactide-co-glycolide); demineralized bone particle; hyaluronic acid; scaffold; spinal cord regeneration;
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