• 한국해양바이오학회지
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• 제14권1호
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• pp.1-8
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• 2022

This study examined the growth, fatty acid (FA) content, and carotenoids of a newly isolated freshwater microalga, Mychonastes sp. 246, in various culture media. The appropriate temperature and light intensity for culturing Mychonastes sp. 246 were determined as 18℃-22℃ and 200-250 µmol/m²/s using a high throughput photobioreactor. The microalgal cells were cultivated in 0.5 L bubble column photobioreactors using BG11, Bold's Basal media, and f/2 media. According to the growth results of the microalgae, BG11, among the tested media, showed the highest biomass concentrations (3.5 ± 0.1 g/L in 10 d). To enhance the biomass growth of the microalgae, the N:P ratio in BG11 was manipulated from 45:1 to 7:1 based on the stoichiometric cell composition. The biomass concentrations of Mychonastes sp. 246 grown on the manipulated BG11 (MBG) increased to 38% (4.6 ± 0.3 g/L in d) compared with the original BG11 (3.3 g/L). The FA content of the microalgae grown on the MBG was lower (8.4%) than that of the original BG11 (10.1%) while the FA compositions did not exhibit any significant differences. Furthermore, three kinds of carotenoids were identified in Mychonastes sp. 246, zeaxanthin, lutein, and β-carotene. These results suggest an effective strategy for increasing biomass concentrations, FA content, and carotenoids of microalgae by performing a simple N:P adjustment in the culture media.

• 주류산업
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• 제26권4호통권90호
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• pp.18-23
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• 2006

• ESCO지
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• 통권16호
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• pp.36-43
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• 2002

• 한국재료학회지
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• 제24권6호
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• pp.310-318
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• 2014

In this experiment, a highly porous scaffold of biphasic calcium phosphate (BCP) was prepared using the spongereplica method. The BCP scaffold was coated with 58S bioactive glass (BG) and sintered for a second time. The resulting scaffold was coated with gelatin (Gel) and cross-linked with [3-(3-dimethyl aminopropyl) carbodiimide] and N-Hydroxysuccinamide (EDC-NHS). The initial average pore size of the scaffold ranged from 300 to $700{\mu}m$, with more than 85 % porosity. The coating of BG and Gel had a significant effect on the scaffold-pore size, decreasing scaffold porosity while increasing mechanical strength. The material and surface properties were evaluated by means of several experiments involving scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) and X-ray diffraction (XRD). Cytotoxicity was evaluated using MTT assay and confocal imaging of MC3T3-E1 pre-osteoblast cells cultured in vitro. Three types of scaffold (BCP, BCP-BG and BCP-BG-Gel) were implanted in a rat skull for in vivo evaluation. After 8 weeks of implantation, bone regeneration occurred in all three types of sample. Interestingly, regeneration was found to be greater (geometrically and physiologically) for neat BCP scaffolds than for two other kinds of composite scaffolds. However, the other two types of scaffolds were still better than the control (i.e., defect without treatment).

• 지역사회간호학회지
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• 제18권2호
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• pp.293-300
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• 2007

Purpose: To examine the effect of the exercise behavior modification therapy one the obesity control and self-esteem of the obese female college students. Method: Data was collected from March 10, 2006 to June 10, 2006. The research design was adopted randomized control group (EG=exercise group) pretest-posttest experimental ($E{\cdot}BG$=exercise${\cdot}$behavior modification therapy group) design. The subjects were nursing students at T college. A total of 37 obese female college students (BMI: over $27mg/m^2$) were selected for this research. The exercise program was executed for 12 weeks and 4 days a week, and the behavior modification was therapy performed for 12 weeks and 60 minutes per week. The data were analyzed with the SPSS Win 12.0 program through the Mann-Whitney test. Result: BMI (Z=-3.049, p=.002) of the $E{\cdot}BG$ was significantly different from the EG. The Total Cholesterol(Z=-1.162, p= .250) of the $E{\cdot}BG$ was not significantly different from the EG. The self-esteem(Z=-3.196, p= .001) of the $E{\cdot}BG$ was significantly different from the EG. Conclusion: The exercise behavior modification therapy was more effective than the exercise therapy in improving the obesity and self-esteem of the obese female college students.

• 드라이브 ㆍ 컨트롤
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• 제16권4호
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• pp.1-8
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• 2019

The HPM (High-speed Power Matching) system is an electro-hydraulic control system. It directly controls the swash plate of the pump by selecting four-loop logic based on joystick signals, pump flow, and pressure signal to improve the efficiency and controllability of construction machines. In the NFC (Negative Flow Control) system, a typical pump control system using conventional open center type MCV, the loss is continuously generated by flow through the center bypass line even when the excavator is not in operation. Also, due to the slow response of the pump that indirectly controls the flow rate using the pressure regulator, peak pressure occurs at the start or stop of the operation. Conversely, the HPM system uses an MCV without center-by-pass flow path and the swash plate of a pump for the HPM is controlled by a high-speed proportional flow control valve. As a result, the HPM system minimizes energy loss in standby state of the excavator and enables peak pressure control through rapid electro-hydraulic control of a pump. In this paper, the concept of the HPM system algorithm is introduced and the hydraulic system efficiency is compared with the NFC system using the excavator SAT (System Analysis Tool).

• 한국유기농업학회지
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• 제6권1호
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• pp.133-149
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• 1997

Recently, the importance of management and cultivation of grasses has been increased in Korea. Among these cultural practices, the appropriate control of diseases is considered more important than other cultivation techniques such as fertilization and irrigation. The damages of brown patch and large patch caused by Rhizoctonia spp. and Pythium blight caused by Pythium spp. are serious in the major cultivation area of turfgrass in Korea. Since these diseases are difficult to control by agrochemicals, the damages are very serious if these are occured. The periodic spray of agrochemicals, to protect and control these diseases could make some problems of toxicity and environmental pollution as well as rising of non-target diseases. Therefore, the biological methods to control diseases have been required to decrease problems resulted from overuse of agrochemicals, to conserve natural ecosystem, and to control effectively diseases of grasses in the long period. The number of studies about biological control using antagonistic microorganisms have been increased for last half century. However, the application of biological control method has been very limited. In this study, thirteen isolates of R. cerealis, 8 isolates of R. solani and 3 isolates of Phthyn spp. have been isolated from diseased turfgrass in golf course and grass-culture area that have patch and wilting symptoms of zoysia grass and creeping bentgrass. Isolation frequency of R. cerealis and R. solani was high in especially zoysiagrass, while Pythym spp. was isolated from bent grass at low frequency but showed high pathogenicity. Totally, 205 isolates of soil microorganisms were isolated in this study as primary antagonistic microorganism by Herr's triple agar layer plate and dual culture method using rhizosphere of grasses, soil of crop field as the source of antagonistic microorganisms. Among the 205 isolates, 23 isolates were actinomycetes and 182 isolates were bacteria. All of the actinomycetes were isolated by Herr's method. Antagonistic effect of primary isolated microorganisms was tested for in vitro mycelial growth inhibition against pathogenic fungi isolated from grasses and for inhibition of disease occurrence in 24 well tissue culture plate and pot experiment. Then, four isolated of bacteria which are BG23, BG74, BG136 and BG171 were selected as antagonistic microorganisms against soil-born pathogenic fungi of bentgrass.

• 문화기술의 융합
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• 제6권4호
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• pp.435-443
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• 2020

이 연구는 블루투스 염도계 사용이 어린이집에서 제공하는 국의 적정 염도 수준을 조절하는 데 영향을 미칠 수 있는지 확인하고자, 2019년 1월부터 12월까지 안양시 어린이급식관리지원센터에 등록된 어린이집 중 나트륨 저감화 특화사업에 참여를 희망하는 어린이집(블루투스 염도계 사용 군, BG)과 참여하지 않은 어린이집(블루투스 염도계 미사용군, NG)을 대상으로 실시하였다. 연구 결과, 블루투스 염도계 사용 여부에 따라 국의 염도와 나트륨양을 비교하였을 때, BG의 값이 NG보다 유의하게 낮았으며, 블루투스 염도계의 사용 횟수가 증가함에 따라 염도와 나트륨양이 감소하였다. 결론적으로, 블루투스 염도계는 어린이집에서의 나트륨 저감화 교육과 관련하여 조리원들의 자발적인 염도 조절을 유도하는 수단으로 활용 가능할 것으로 사료된다.

• 문화기술의 융합
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• 제6권4호
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• pp.45-52
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• 2020

이 연구는 회화 및 울금으로 단독 염색한 견직물 그리고 쪽과 회화 및 쪽과 울금으로 복합 염색한 견직물의 염색성 및 항균성에 대하여 검토한 것이다. 회화 및 울금으로 단독 염색한 견직물의 Munsell 표색계의 색상은 각각 5.0Y 및 0.6Y로 노란색 계열을, 쪽과 회화 그리고 쪽과 울금 추출액으로 복합 염색한 견직물의 색상은 각각 2.6BG 및 1.5BG로 청록색 계열을 나타내었다. 쪽과 회화로 복합 염색한 견직물의 일광견뢰도는 3등급으로 비교적 양호하였고, 드라이클리닝견뢰도 및 마찰견뢰도의 경우, 네 종류의 염색 견직물 모두 4~5등급으로 우수하게 나타났다. 회화 추출액으로 염색한 견직물의 Staphylococcus aureus에 대한 항균활성은 99.5% 그리고 나머지 세 종류의 염색 견직물은 99.9%의 뛰어난 항균성을 나타내었다. 또한 Klebsiella pneumonia에 대한 항균활성에 있어서도 회화 추출액으로 염색한 견직물은 99.6%, 그 나머지 세 종류의 염색 견직물은 99.9%의 우수한 항균성을 나타내었다.

• 한국수소및신에너지학회논문집
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• 제21권2호
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• pp.104-110
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• 2010

Photobiological hydrogen production by nitrogen-fixing unicellular cyanobacteria has long been considered to be an environmentally sound and very promising method for the future supply of renewable clean energy. We tried to find out the optimum cell concentration for $H_2$ production in each of the two new Korean nitrogen-fixing unicellular cyanobacterial strains to compare with Synechococcus sp. strain Miami BG043511. The two Korean strains, Cyanothece sp. KNU CB MAL-031 and KNU CB MAL-058, were isolated from Korean west coasts. Cell concentrations up to 17 billion cells $ml^{-1}$ were applied to the tests. High cell concentration over 15 billion cells $ml^{-1}$ resulted in drastically reduced $H_2$ production in all the three strains. The two domestic strains, however, produced 2-3 time more hydrogen than Synechococcus sp. Miami BG043511 at cell concentrations of 5-10 billion cells $ml^{-1}$. At lower cell concentrations than 2 billion cells $ml^{-1}$, MAL-031 exhibited highest $H_2$ production followed by Miami BG043511, with far less production in MAL-058. Present result suggests that Cyanothece sp. MAL-CB031 might be one of the ideal nitrogen-fixing unicellular cyanobacterial strains for the photobiological hydrogen production.