• Title/Summary/Keyword: BALB/c-mouse

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Effect of Herb Extracts Mixed with Houttuynia Cordata on Antiatopic Dermatitis in DNCB-Induced BALB/c Mouse (DNCB 유도 BALB/c 생쥐에서 어성초 혼합 한방추출물의 항아토피 효과)

  • Park, Sang-Oh;Park, Byung-Sung;Ryu, Chae-Min;Ahn, Yong-Sik
    • Journal of the Korean Applied Science and Technology
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    • v.29 no.2
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    • pp.175-183
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    • 2012
  • This study was evaluated the antiatopic activity of cream containing a herb extracts mixed with Houttuynia cordata in DNCB-induced BALB/c atopy mouse. The randomized complete block design was done by BALB/c mouse into two groups, of which the control group with atopy and the cream treatment group. It was found that each level of IgE and histamine in blood was significantly decreased in the cream treatment group, compared with the DNCB-induced atopy control group. When the cream was applied to the atopy mouse, it could be observed that its skin recovered to normal condition with the skin surface being clean and smooth without any horny tissue. The results suggest that the application of herb extracts mixed with Houttuynia cordata has an antiatopic activity through a inhibition of histamin emissions with reducing the levels of blood IgE in BALB/C atopy mouse.

Effect of Immune System on Retrovirus-Mediated Herpes Simplex Virus Thymidine Kinase Gene Therapy (면역체계가 Retroviral Vector로 이입한 Herpes Simplex Virus Thymidine Kinase 유전자치료에 미치는 영향)

  • Park, Jae-Yong;Joo, So-Young;Chang, Hee-Jin;Son, Ji-Woong;Kim, Kwan-Young;Kim, Keong-Seok;Kim, Chang-Ho;Park, Jae-Ho;Lee, Jong-Ki;Jung, Tae-Hoon
    • Tuberculosis and Respiratory Diseases
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    • v.46 no.2
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    • pp.229-240
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    • 1999
  • Background: The impact of the immune response on cancer gene therapy using viral vectors to deliver a "suicide gene" is currently unclear. A vigrous immune response targeted at viral proteins or transgene may enhance the efficacy of tumor destruction and even augment responses to tumor antigens. These responses may involve the release of cytokines and stimulation of tumor specific cytotoxic T-lymphocytes that enhance therapeutic efficacy. On the other hand, a vigorous rapid cellular immune response may destroy cells expressing the therapeutic gene and attenuate the response to therapy. Furthermore, development of neutralizing antibody responses may prevent readministration of virus, a potentially significant limitation. Evaluating the significance of these limitations in animal models and developing solutions are therefore of obvious importance. Methods: After retroviral transduction of mouse mesothelioma cell line(AB12) with Herpes Simplex Virus thymidine kinase (HSVtk) gene in vitro, subcutaneous flank tumors were established. To study the effect of intact immune system on efficacy of tumor erradication, the ability of the HSVtk/ganciclovir system to inhibit tumor growth was compared among normal Balb/c mice, immunodeficient Balb/c-nude and SCID mice, and Balb/c mice immunosuppressed with cyclosporin. Results: Ganciclovir treatment resulted in greater inhibition of tumor growth in Balb/c mice compared with immunodeficient Balb/c-nude mice and SCID mice(in immunodeficient mice, there were no growth inhibition by ganciclovir treatment). Ganciclovir treatment resulted in greater inhibition of tumor growth in noncyclosporin (CSA) treated Balb/c mice compared with CSA treated Balb/c mice. On day 8, mean ganciclovir-treated tumor volume were 65% of control tumor volume in Balb/c mice versus 77% control tumor volume in CSA-treated Balb/c mice. This effect was still evident during therapy (day 11 and 13). On day 13, non-CSA treated tumor volume was 35% of control tumor volume versus 60% of control tumor volume in CSA treated Balb/c mice. Duration of expression of HSVtk was not affected by the immunosuppression with CSA. Conclusion: These results indicate that the immune responses against retrovirally transduced cells enhance the efficacy of the HSVtk/ganciclovir system. These findings have important implications for clinical trials using currently available retrovirus vectors as well as for future vector design.

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Cytotoxicity of water extract of Dangkwieumja ka Sumsoo on A43l Cells (當歸飮子加蟾수가 皮膚癌細胞(A431)의 細胞毒性에 미치는 影響)

  • Choi, Jeong-Hwa
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.9 no.1
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    • pp.1-15
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    • 1996
  • The purpose of this research was to investigate effect of water extract of DangKwi-Eum-Ja ka Sumsoo(DESE) on the cytotoxicity of human epidemloid cell, A431 cells. The effects of DESE on the proliferation of A431 cells, Balb/c 3T3 cells, mouse thymocytes and splenocnes were estimated by MTT colorimetric assay, and nitric oxide production from mouse peritoneal macrophage was estimated by Griess method. DESE inhibited the proliferation of A431 cells at $10{\mu}g/ml$, and did not affect the proliferation of Balb/c 3T3 cells. DESE decreased the cytotoxicity of mitomycin C or cisplatin on A431 cells, increased the cytotoxicity of mitomycin C or cisplatin on Balb/c 3T3 cells. DESE inhibited the proliferation of mouse thymocytes and splenocytes at $100{\mu}g/ml$. DESE did not affect the nitric oxide production from mouse peritoneal macrophage in vitro, but decreased the nitric oxide production from DESE-treated mouse peritoneal macrophage.

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Expression of cytokines and co-stimulatory molecules in the Toxoplasma gondii-infected dendritic cells of C57BL/6 and BALB/c mice

  • Jae-Hyung Lee;Jae-Min Yuk;Guang-Ho Cha;Young-Ha Lee
    • Parasites, Hosts and Diseases
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    • v.61 no.2
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    • pp.138-146
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    • 2023
  • Toxoplasma gondii is an intracellular protozoan parasite which can infect most warm-blooded animals and humans. Among the different mouse models, C57BL/6 mice are more susceptible to T. gondii infection compared to BALB/c mice, and this increased susceptibility has been attributed to various factors, including T-cell responses. Dendritic cells (DCs) are the most prominent type of antigen-presenting cells and regulate the host immune response, including the response of T-cells. However, differences in the DC responses of these mouse strains to T. gondii infection have yet to be characterized. In this study, we cultured bone marrow-derived DCs (BMDCs) from BALB/c and C57BL/6 mice. These cells were infected with T. gondii. The activation of the BMDCs was assessed based on the expression of cell surface markers and cytokines. In the BMDCs of both mouse strains, we detected significant increases in the expression of cell surface T-cell co-stimulatory molecules (major histocompatibility complex (MHC) II, CD40, CD80, and CD86) and cytokines (tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-12p40, IL-1β, and IL-10) from 3 h post-T. gondii infection. The expression of MHC II, CD40, CD80, CD86, IFN-γ, IL-12p40, and IL-1β was significantly higher in the T. gondii-infected BMDCs obtained from the C57BL/6 mice than in those from the BALB/c mice. These findings indicate that differences in the activation status of the BMDCs in the BALB/c and C57BL/6 mice may account for their differential susceptibility to T. gondii.

Estimation of Combining Abilities for Traits of Mice from Diallel Crosses -I. Estimation of Combining Abilities for Litter Size and Birth Weights of Mice from Diallel Crosses (양면교잡(兩面交雜)에 의(依)한 Mouse 주요(主要) 형질(形質)의 결합능력(結合能力) 추정(推定) -I. 산자수(産仔數) 및 생시체중(生時体重)에 대(對)한 결합능력(結合能力) 추정(推定))

  • Hyun, Byung Hwa;Choi, Kwang Soo
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.114-118
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    • 1986
  • The study was conducted to find out the gene effects on litter size and birth weights in mice with 362 progenies from full-diallel crosses of four lines of BALB/c, CBA, C3H and C57BL. The progenies were farrowed at the Experimental Animal Farm, College of Agriculture, Kyungpook National University in November, 1984, and data were analyzed into general combining ability, specific combining ability and reciprocal effects with Griffing's model. General combining ability effects estimated in line-crosses were -0.4163~0.3337 for litter size and -0.0356~0.0894 for birth weights. However, no significant differences were observed in general combining ability effects on litter size and birth weights. Specific combining ability effects estimated in line-crosses were -1.0388~1.7913 for litter size and -0.1144~0.1343 for birth weights. However, the specific combining ability effects for litter size and birth weights appeared to be insignificant. The reciprocal effects, which appeared to be significant, were -2.26 from BALB/c ${\times}$ C3H, 1.84 from CBA ${\times}$ C57BL and -1.50 from BALB/c ${\times}$ CBA for litter size. For birth weights, the reciprocal effects were estimated -0.26 from CBA ${\times}$ C57BL, 0.15 from BALB/c ${\times}$ CBA and -0.15 from BALB/c ${\times}$ C57BL.

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Cytotoxicity of Petroleum Ether Extract of Euonymus alatus (귀전우 Petroleum Ether 추출물의 세포독성)

  • Eun, Jae-Soon;Park, Sang-Ho;Kweon, Jin;Kim, Young-Ahn;Kang, Sung-Young;Oh, Chan-Ho;Jeon, Hoon
    • Korean Journal of Pharmacognosy
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    • v.26 no.3
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    • pp.253-258
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    • 1995
  • The purpose of this research was to investigate the effects of petroleum ether extract of Euonymus alatus (EAP) on the proliferation of human tumor cells. EAP inhibited the proliferation of HeLa, Hep G2, KHOS/NP and A431 cells. The cytotoxicity of doxorubicin on human tumor cells and Balb/c 3T3 cells were increased by the combination of EAP. EAP did not affect the proliferation of Balb/c 3T3 cells, mouse spleen cells and human lymphocytes. These results suggest that EAP has the cytotoxicity on human tumor cells without cytotoxicity on Balb/c 3T3 cells, mouse spleen cells and human lymphocytes, and increase the cytotoxicity of doxorubicin.

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Bone Marrow Progenitors and IL-2 Signaling Contribute to the Strain Differences of Kidney Innate Lymphoid Cells

  • Seungwon Ryu;Hye Young Kim
    • IMMUNE NETWORK
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    • v.23 no.2
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    • pp.15.1-15.17
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    • 2023
  • Innate lymphoid cells (ILCs) are critical immune-response mediators. Although they largely reside in mucosal tissues, the kidney also bears substantial numbers. Nevertheless, kidney ILC biology is poorly understood. BALB/c and C57BL/6 mice are known to display type-2 and type-1 skewed immune responses, respectively, but it is unclear whether this extends to ILCs. We show here that indeed, BALB/c mice have higher total ILCs in the kidney than C57BL/6 mice. This difference was particularly pronounced for ILC2s. We then showed that three factors contributed to the higher ILC2s in the BALB/c kidney. First, BALB/c mice demonstrated higher numbers of ILC precursors in the bone marrow. Second, transcriptome analysis showed that compared to C57BL/6 kidneys, the BALB/c kidneys associated with significantly higher IL-2 responses. Quantitative RT-PCR also showed that compared to C57BL/6 kidneys, the BALB/c kidneys expressed higher levels of IL-2 and other cytokines known to promote ILC2 proliferation and/or survival (IL-7, IL-33, and thymic stromal lymphopoietin). Third, the BALB/c kidney ILC2s may be more sensitive to the environmental signals than C57BL/6 kidney ILC2s since they expressed their transcription factor GATA3 and the IL-2, IL-7, and IL-25 receptors at higher levels. Indeed, they also demonstrated greater responsiveness to IL-2 than C57BL/6 kidney ILC2s, as shown by their greater STAT5 phosphorylation levels after culture with IL-2. Thus, this study demonstrates previously unknown properties of kidney ILC2s. It also shows the impact of mouse strain background on ILC2 behavior, which should be considered when conducting research on immune diseases with experimental mouse models.

Egg Production of Clonorchis sinensis in Different Strains of Inbred Mice (근교계 마우스에서 간흡충 기생기간과 산란력의 변동)

  • Kim, Jong-In;Jeong, Dong-Il;Choe, Dong-Ik
    • Parasites, Hosts and Diseases
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    • v.30 no.3
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    • pp.169-176
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    • 1992
  • In order to compare the intraspecific variation in host-parasite relationship of Clonorchis sinensis, six strains of inbred mice, ICR, DDY, GPC, BALB/c, nude and DS, were infected orally with 20 metacercariae of C. sinensis. The biologic incubation period of C. sinensis was the shortest in DDY mice, 21.2 days in average, followed by GPC 21.4, BALB/c and DS 23.2, ICR and nude 23.4 days, respectively. The fertile period of the cuke was also the longest in the DDY strain, 164 days on average, followed by GPC 132, BALB/c 97, nude 37, DS 32 and ICR 28 days. The egg-laying capacity of the cuke in DDY and GPC was relatively high and stable compared with the other four strains of mice. It was found that there are intraspecific variations in biologic incubation period, fertile period, and fecundity of C. sinensis. The DDY mouse is likely to be the most suitable experimental animal among the six strains of the mice tested. Key words: Mouse strain, Clonorchis sinensis, egg-laying capacity.

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마우스의 흉선에 미치는 7,12-Dimethylbenz[a]anthrancene의 영향

  • 이덕윤
    • Toxicological Research
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    • v.9 no.1
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    • pp.1-11
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    • 1993
  • This study was carried out to investigate the immunopathological effects of 7, 12-Dimethylbenz[a]anthracene(DMBA) on mouse thymus. DMBA was administered subcutaneously to BALB/C mice by interscapular single injection of 50 or 100 ng/g of body weight. Each DMBA treatment group and additional corn oil control group of mice were studied on day 1, 3, 7, 14 and 21 following the injection of DMBA. DMBA treatment resulted in marked decreases in weights and cellularity of thymus. Thymus weights were decreased by 50.6 and 66.0% at 21 days, respectively, after treatment of 50ng/g and 100 ng/g DMBA.

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Temporal changes of the activity of catalase, superoxide dismutase, and glutathione peroxidase in BALB/c mice skin after a single dose UVB irradiation (UVB 1회 조사 후 시간에 따른 BALB/c마우스의 피부 항산화효소 활성도 변화)

  • Lee, Joung-Hee;Park, Kyoung-Ae;Lee, Hee-Joo;Park, Myoung-Sook;Jeon, Sang-Eun;Park, Kyoung-Chan;Choi, S-Mi
    • Journal of Korean Biological Nursing Science
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    • v.3 no.1
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    • pp.53-61
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    • 2001
  • Skin is constantly exposed to air, solar radiation, ozone and other air pollutants formulating free radicals. The reactive oxygen species(ROS), formed under these conditions, are associated with skin cancers, cutaneous photoaging, and cutaneous inflammatory disorders. In this study, we sought to establish an animal model for UVB-induced skin alteration using BALB/c mice. The level of UVB irradiation used in this model was within physiological dose. BALB/c mice were exposed to a single dose of UVB ($200mJ/cm^2$ and were sacrificed at 3, 6, 24, and 48 hours following the irradiation. The effect of a single exposure to UVB irradiation on skin catalase(CAT), superoxide dismutase(SOD), and glutathione peroxidase(GPx) activities were examined. Significant decrease in the activity of all enzymes were observed at 6 hours after irradiation(p<.05). The activity of CAT decreased more sharply than those of SOD and GPx, and then remained depressed until 48 hours after UVB irradiation, whereas the activity of GPx recovered to basal level at 48 h after UVB irradiation. Our results indicate that BALB/c mouse could be an adequate animal model of UVB irradiation experiment. These results will also provide fundamental knowledge for the effective nursing strategies in reducing UV-induced skin disorders.

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