• 제목/요약/키워드: BAL fluid

검색결과 119건 처리시간 0.03초

중합반응을 이용한 흰쥐 페포자충증의 진단 (PCR in diagnosis of pneumocystosis of rats)

  • 홍성태
    • Parasites, Hosts and Diseases
    • /
    • 제34권3호
    • /
    • pp.191-196
    • /
    • 1996
  • 중합반응(W_R)은 극미량의 핵산을 찾아내어 소수의 감염병원체를 확인하는 매우 민감한 진단법이다. 폐포자충 같이 다수의 숙주세포에 소수의 병원제가 섞여 있는 가검물에서 핵산의 정제 여부에 따른 중합반응의 민감도를 관찰하고. 특이도가 높은 시발제(primer)를 개발하기 위하여 이 연구를 수행하였다 흰쥐를 실험적으로 감염시키고 폐 폐포세척액. 혈청을 화보하여 현미경적 검사와 중합반응을 실시하였다 또한 사람과 횐쥐의 핵산을 위시하여 여러 미생물과 기생충. 이스트 의 핵산을 절제하여 이 시발체의 특이도를 검증하였다. 그 결과 여러 시발체 중에서 rRNA의 염기 서열 중에서 선택한 #24 주서열과 #27 대서열 쌍이 가장 우수한 민감도와 특이도를 보였다. 형태학적으로 양성인 폐포세척액의 세포용해액으로 반응시킨 경우 민감도가 57.7%이며 핵산을 정제한 경우 84.6%로 증가하였다 병원체 음성인 경우와 다른 병원체와 숙주의 핵산과는 반응하지 않았다. 혈청을 이용한 경우 20개 양성 표본 중 2개가 양성이고 6개의 감염된 흰쥐의 혈액은 모두 음성이었다. 충합반응을 폐포자충증의 진단에 활용하기 위하여는 폐포세척액 보다는 가래나 기관지 분비물. 혈청이나 혈액같은 비침습적인 가검물을 이용하고 핵산시료를 준비하는 과정이 간편하고 재현성이 있도록 개발되어야 할 것이다.

  • PDF

비강내 점적 노출을 통한 산화 알루미늄 나노입자의 폐독성 평가 (Pulmonary Toxicity Assessment of Aluminum Oxide Nanoparticles via Nasal Instillation Exposure)

  • 권정택;서균백;이미미;김현미;심일섭;조은혜;김필제;최경희
    • 한국환경보건학회지
    • /
    • 제39권1호
    • /
    • pp.48-55
    • /
    • 2013
  • Objective: The use of nanoparticle products is expected to present a potential harmful effect on consumers. Also, the lack of information regarding inhaled nanoparticles may pose a serious problem. In this study, we addressed this issue by studying pulmonary toxicity after nasal instillation of Al-NPs in SD rats. Methods: The animals were exposed to Al-NPs at 1 mg/kg body weight (low dose), 20 mg/kg body weight (medium dose) and 40 mg/kg body weight (high dose). To determine pulmonary toxicity, bronchoalveolar lavage (ts.AnBAL) fluid analysis and histopathological examination were conducted in rats. In addition, cell viability was investigated at 24 hours after the treatment with Al-NPs. Results: BAL fluid analysis showed that total cells (TC) count and total protein (TP) concentrations increased significantly in all treatment groups, approximately two to three times. Also, lactate dehydrogenase (LDH) and cytokines such as TNF-alpha and IL-6 dose-dependently increased following nasal instillation of Al-NPs. However, polymorphonuclear leukocytes (PMNs) levels showed no significant changes in a dose dependant manner in BAL fluid. In the cytotoxicity analysis, the treatment of Al-NPs significantly and dose-dependently induced cell viability loss (20 to 30%) and damage of cell membrane (5 to 10%) in rat normal lung epithelial cells (L2). Conclusions: Our results suggest that inhaled Al-NPs in the lungs may be removed quickly by alveolar macrophages with minimal inflammatory reaction, but Al-NPs have the potential to affect lung permeability. Therefore, extensive toxicity evaluations of Al-NPs are required prior to their practical application as consumer products.

기관지 폐포 세정액의 세포학적 소견 (Cytologic Findings of Bronchoalveolar Lavage)

  • 권건영;조승제;박관규;장은숙
    • 대한세포병리학회지
    • /
    • 제1권2호
    • /
    • pp.129-138
    • /
    • 1990
  • Bronchoalveolar lavage (BAL) has emerged as a useful technique for the study of pulmonary interstitial disorders. Several types of Information are provided by the evaluation of lavage fluid identification of cellular constituents helps to separate inflammatory process. Recently we have studied cellular constituents of BAL from three cases with histologically confirmed pulmonary sarcoidosis, idiopathic pulmonary fibrosis and hypereosinophilic syndrome. Pulmonary sarcoidosis showed a marked increase in lymphocytes, idiopathic pulmonary fibrosis revealed a predominance of neutrophils, and hypereosinophilic syndrome presented a marked increase in eosinophils in the lavage fluids.

  • PDF

PAF Contributes to Intestinal Ischemia/Reperfusion-Induced Acute Lung Injury through Neutrophilic Oxidative Stress

  • Lee, Young-Man;Park, Yoon-Yub
    • The Korean Journal of Physiology and Pharmacology
    • /
    • 제3권4호
    • /
    • pp.405-414
    • /
    • 1999
  • The role of platelet-activating factor (PAF) was investigated in intestinal ischemia/reperfusion (I/R) induced acute lung injury associated with oxidative stress. To induce acute lung injury following intestinal I/R, superior mesenteric arteries were clamped with bulldog clamp for 60 min prior to the 120 min reperfusion in Sprague-Dawley rats. Acute lung injury by intestinal I/R was confirmed by the measurement of lung leak index and protein content in bronchoalveolar lavage (BAL) fluid. Lung leak and protein content in BAL fluid were increased after intestinal I/R, but decreased by WEB 2086, the PAF receptor antagonist. Furthermore, the pulmonary accumulation of neutrophils was evaluated by the measurement of lung myeloperoxidase (MPO) activity and the number of neutrophils in the BAL fluid. Lung MPO activity and the number of neutrophils were increased (p<0.001) by intestinal I/R and decreased by WEB 2086 significantly. To confirm the oxidative stress induced by neutrophilic respiratory burst, gamma glutamyl transferase (GGT) activity was measured. Lung GGT activity was significantly elevated after intestinal I/R (p<0.001) but decreased to the control level by WEB 2086. On the basis of these experimental results, phospholipase $A_2\;(PLA_2),$ lysoPAF acetyltransferase activity and PAF contents were measured to verify whether PAF is the causative humoral factor to cause neutrophilic chemotaxis and oxidative stress in the lung following intestinal I/R. Intestinal I/R greatly elevated $PLA_2$ activity in the lung as well as intestine (p<0.001), whereas WEB 2086 decreased $PLA_2$ activity significantly (p<0.001) in both organs. LysoPAF acetyltransferase activity, the PAF remodelling enzyme, in the lung and intestine was increased significantly (p<0.05) also by intestinal I/R. Accordingly, the productions of PAF in the lung and intestine were increased (p<0.001) after intestinal I/R compared with sham rats. The level of PAF in plasma was also increased (p<0.05) following intestinal I/R. In cytochemical electron microscopy, the generation of hydrogen peroxide was increased after intestinal I/R in the lung and intestine, but decreased by treatment of WEB 2086 in the lung as well as intestine. Collectively, these experimental results indicate that PAF is the humoral mediator to cause acute inflammatory lung injury induced by intestinal I/R.

  • PDF

창이자(蒼耳子)가 제 I 형 알레르기 천식(喘息)모델 흰쥐의 BALF내(內) 면역세포(免疫細胞) 및 혈청(血淸) IgE에 미치는 영향(影響) (The Effect of Changiga on Immune Cell & Serum OA-specific IgE in BALF in Rat Asthma Model)

  • 이병희;이상재;김광호
    • 대한예방한의학회지
    • /
    • 제5권2호
    • /
    • pp.93-105
    • /
    • 2001
  • BACKGROUND : Changiga is a hetnal medicine which has been used of the traditional therapeutic agent of asthma. So I examine the effect of Changija on immune Cell&serum OA-specific IgE in BALF in rat asthma model. MATERIAL and METHODS : Rats were sensitized with OA; at day 1 sensitized group and Changiga(CIG) groups were systemically immunized by subcutaneous ingection of 1mg OA and 300mg of Al(OH)3 in a total volume of 2ml. At the same time, 1ml of 0.9% saline containing $6{\times}109$ B. pertussis bacilli was injected by i.p. 14 days, after the systemic immunization, rats received local immunization by inhaling 0.9% saline aerocol containing 2%(wt/vol) OA, A day after local immunization, BAL fluid was collected from the rats. A day after local immunization, rats were orally administered with Changiga extract 14 days, Lymphocyte, CD4+ T-cell CD8+ T-cell counts, CD4+/CD8+ ratio in BALF, change of serum OA-specific IgE level in the peripheral blood were measured and evaluated. RESULT : Changiga showed a suppressive effect on a rat asthme model. Changiga decreased lymphocyte, CD4+ T-cell, CD4+/CD8+ ratio in BALF, serum OA-specific IgE level as compared with the control group, whereas Changiga decreased CD8+ T-cell in BALF with statistical nonsignificance as compared with the control group. CONCLUSION: This study shows that Changiga have a suppressive effect on rat allergic athma model. Changiga would be useful allergic asthma treatment agent.

  • PDF

호도(胡桃) 물추출물이 Ovalbumin으로 유도된 천식동물모델에 미치는 영향 (The Effects of Juglandis Semen Extract on the Experimental Asthma induced by Ovalbumin)

  • 김상찬;변성희
    • 대한한의학방제학회지
    • /
    • 제13권1호
    • /
    • pp.145-159
    • /
    • 2005
  • Objective : Juglandis Semen has a function that to invigorate the lung and kidney. And It is commonly used as a supporting agent in the treatment of coughing and bronchitis. This study was performed to investigate the effect of oral administration of Juglandis Semen Extract (JSE) against the experimental asthma induced by ovalbumin. Methods : Asthma was induced to Balb/c mouse by i.p. injection and aerosol immunization with ovalbumin. It was observed the change of the cell number in the BAL fluid. Concentrations of IL-4, IL-5 in splenoc yte were assessed by ELISA, IgG and IgE from serum were calculated by same method. Results : 1. Number of macrophage in BAL fluid was significantly decreased in JSE group compared with control group, but not eosinophil and lymphocyte. 2. Levels of IgG and IgE in serum were significantly decreased in JSE group compared with control group, respectively. 3. Concentration of IL-4 in culture supernatant of splenocyte was significantly decreased in JSE group compared with control group, but there was no significant in IL-5. Conclusion : We found that the effect of JSE extract in asthma was implicated in reduction of IL4released from Th2 cell, and decreases of IgG and IgE from plasma cell. These findings suggest that JSE can produce anti-asthmatic effect, which may play a role in allergen-induced asthma therapy.

  • PDF

Free-Living Amoeba Vermamoeba vermiformis Induces Allergic Airway Inflammation

  • Lee, Da-In;Park, Sung Hee;Kang, Shin-Ae;Kim, Do Hyun;Kim, Sun Hyun;Song, So Yeon;Lee, Sang Eun;Yu, Hak Sun
    • Parasites, Hosts and Diseases
    • /
    • 제60권4호
    • /
    • pp.229-239
    • /
    • 2022
  • The high percentage of Vermamoeba was found in tap water in Korea. This study investigated whether Vermamoeba induced allergic airway inflammation in mice. We selected 2 free-living amoebas (FLAs) isolated from tap water, which included Korean FLA 5 (KFA5; Vermamoeba vermiformis) and 21 (an homolog of Acanthamoeba lugdunensis KA/E2). We axenically cultured KFA5 and KFA21. We applied approximately 1×106 to mice's nasal passages 6 times and investigated their pathogenicity. The airway resistance value was significantly increased after KFA5 and KFA21 treatments. The eosinophil recruitment and goblet cell hyperplasia were concomitantly observed in bronchial alveolar lavage (BAL) fluid and lung tissue in mice infected with KFA5 and KFA21. These infections also activated the Th2-related interleukin 25, thymic stromal lymphopoietin, and thymus and activation-regulated chemokines gene expression in mouse lung epithelial cells. The CD4+ interleukin 4+ cell population was increased in the lung, and the secretion of Th2-, Th17-, and Th1-associated cytokines were upregulated during KFA5 and KFA21 infection in the spleen, lung-draining lymph nodes, and BAL fluid. The pathogenicity (allergenicity) of KFA5 and KFA21 might not have drastically changed during the long-term in vitro culture. Our results suggested that Vermamoeba could elicit allergic airway inflammation and may be an airway allergen.

Cathelicidin-related Antimicrobial Peptide Contributes to Host Immune Responses Against Pulmonary Infection with Acinetobacter baumannii in Mice

  • Min-Jung Kang;Ah-Ra Jang;Ji-Yeon Park;Jae-Hun Ahn;Tae-Sung Lee;Dong-Yeon Kim;Do-Hyeon Jung;Eun-Jung Song;Jung Joo Hong;Jong-Hwan Park
    • IMMUNE NETWORK
    • /
    • 제20권3호
    • /
    • pp.25.1-25.13
    • /
    • 2020
  • Acinetobacter baumannii is known for its multidrug antibiotic resistance. New approaches to treating drug-resistant bacterial infections are urgently required. Cathelicidin-related antimicrobial peptide (CRAMP) is a murine antimicrobial peptide that exerts diverse immune functions, including both direct bacterial cell killing and immunomodulatory effects. In this study, we sought to identify the role of CRAMP in the host immune response to multidrug-resistant Acinetobacter baumannii. Wild-type (WT) and CRAMP knockout mice were infected intranasally with the bacteria. CRAMP-/- mice exhibited increased bacterial colony-forming units (CFUs) in bronchoalveolar lavage (BAL) fluid after A. baumannii infection compared to WT mice. The loss of CRAMP expression resulted in a significant decrease in the recruitment of immune cells, primarily neutrophils. The levels of IL-6 and CXCL1 were lower, whereas the levels of IL-10 were significantly higher in the BAL fluid of CRAMP-/- mice compared to WT mice 1 day after infection. In an in vitro assay using thioglycollate-induced peritoneal neutrophils, the ability of bacterial phagocytosis and killing was impaired in CRAMP-/- neutrophils compared to the WT cells. CRAMP was also essential for the production of cytokines and chemokines in response to A. baumannii in neutrophils. In addition, the A. baumannii-induced inhibitor of κB-α degradation and phosphorylation of p38 MAPK were impaired in CRAMP-/- neutrophils, whereas ERK and JNK phosphorylation was upregulated. Our results indicate that CRAMP plays an important role in the host defense against pulmonary infection with A. baumannii by promoting the antibacterial activity of neutrophils and regulating the innate immune responses.

랫드에 주입된 세피오라이트에 의한 폐기능 변화 및 생체내구성 평가 (Changes of Pulmonary Function and Biopersistence Evaluation of Sepiolite in Sprague-Dawely Rats)

  • 정용현;한정희;성재혁;유일재
    • 한국산업보건학회지
    • /
    • 제16권4호
    • /
    • pp.303-313
    • /
    • 2006
  • Two kinds of sepiolite (sepiolite 1, sepiolite 2), a $500^{\circ}C$ heat treated sepiolite (sepiolite 500), and a $700^{\circ}C$ heat treated sepiolite (sepiolite 700) were analyzed for their physicochemical properties. After these sepiolites were instilled into rat lungs, the effects of these substances on lung function and biochemical changes were evaluated. In addition, the fibers in the lungs were counted and characterized after the lungs were treated for electron microscopical analysis. The lungs instilled with sepiolites increased their weight and tidal volume statistically significantly compared with the unexposed control. The numbers of lymphocytes and polymorphonuclear cells (PMN) in the bronchoalveolar lavage (BAL) fluid also increased compared with the control, indicating the sepiolite induced inflammation. The heat treated sepiolites, however, did not show any toxicological differences from the untreated sepiolites. Although sepiolite showed less change in fiber atomic % compositions ( sepiolite 500, Si 0.9%, p <0.01 ; sepiolite 700, Si 3.7%, p<0.05) than chrysotile (Si 9.7%, p<0.01), the durability of the fibers in the lungs could not be determined in this subchronic experiment.

Pneumocystis carinii 폐렴의 기관지 폐포세정액: 세포학적 및 전자현미경적 소견 (Bronchoalveolar Lavage of Pneumocystis carinii Pneumonia: Cytological and Ultrastructural Features)

  • 권건영;윤철희;김상표;박관규;장은숙
    • 대한세포병리학회지
    • /
    • 제5권1호
    • /
    • pp.1-9
    • /
    • 1994
  • The cytological and ultrastructural findings of Pneumocystis carinii(PC) obtained from rats by bronchoalveolar lavage (BAL) are described. All developmental forms of the PC organisms were obtained in the lavage fluid. Papanicolaou stain revealed conglomeration of PC as a foamy cast. The cystic walls of PC were well identified on Gomori's methenamine silver stain. Trophozoites and intracystic bodies were stained by Giemsa and Diff-Quik techniques. Some PC organisms were seen within the alveolar macrophages. Ultrastructurally, the cysts were almost circular in shape, and were nearly devoid of surface tubular extensions. The wall of the cyst was composed of an unit membrane, an intermediate electron lucent layer and an external electron dense layer The cysts frequently contained intracystic bodies, so called sporozoites. Occasionally empty or collapsed cysts with no intracystic bodies, and precysts were found. Trophozoites were variable in size and shape with abundant tubular extensions along the single electron dense pellicle. BAL is a useful method for concentrating the various morphologic forms of PC organisms, and is a rapid diagnostic method for PC pneumonia.

  • PDF