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http://dx.doi.org/10.3347/kjp.2022.60.4.229

Free-Living Amoeba Vermamoeba vermiformis Induces Allergic Airway Inflammation  

Lee, Da-In (Department of Parasitology and Tropical Medicine, School of Medicine, Pusan National University)
Park, Sung Hee (Department of Parasitology and Tropical Medicine, School of Medicine, Pusan National University)
Kang, Shin-Ae (Department of Parasitology and Tropical Medicine, School of Medicine, Pusan National University)
Kim, Do Hyun (Department of Premedicine, School of Medicine, Pusan National University)
Kim, Sun Hyun (Department of Premedicine, School of Medicine, Pusan National University)
Song, So Yeon (Department of Premedicine, School of Medicine, Pusan National University)
Lee, Sang Eun (Department of Premedicine, School of Medicine, Pusan National University)
Yu, Hak Sun (Department of Parasitology and Tropical Medicine, School of Medicine, Pusan National University)
Publication Information
Parasites, Hosts and Diseases / v.60, no.4, 2022 , pp. 229-239 More about this Journal
Abstract
The high percentage of Vermamoeba was found in tap water in Korea. This study investigated whether Vermamoeba induced allergic airway inflammation in mice. We selected 2 free-living amoebas (FLAs) isolated from tap water, which included Korean FLA 5 (KFA5; Vermamoeba vermiformis) and 21 (an homolog of Acanthamoeba lugdunensis KA/E2). We axenically cultured KFA5 and KFA21. We applied approximately 1×106 to mice's nasal passages 6 times and investigated their pathogenicity. The airway resistance value was significantly increased after KFA5 and KFA21 treatments. The eosinophil recruitment and goblet cell hyperplasia were concomitantly observed in bronchial alveolar lavage (BAL) fluid and lung tissue in mice infected with KFA5 and KFA21. These infections also activated the Th2-related interleukin 25, thymic stromal lymphopoietin, and thymus and activation-regulated chemokines gene expression in mouse lung epithelial cells. The CD4+ interleukin 4+ cell population was increased in the lung, and the secretion of Th2-, Th17-, and Th1-associated cytokines were upregulated during KFA5 and KFA21 infection in the spleen, lung-draining lymph nodes, and BAL fluid. The pathogenicity (allergenicity) of KFA5 and KFA21 might not have drastically changed during the long-term in vitro culture. Our results suggested that Vermamoeba could elicit allergic airway inflammation and may be an airway allergen.
Keywords
Vermamoeba; Acanthamoeba; free living amoeba; allergic airway inflammation;
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1 Corsaro D, Venditti D. Detection of novel Chlamydiae and Legionellales from human nasal samples of healthy volunteers. Folia Microbiol (Praha) 2015; 60: 325-334. https://doi.org/10.1007/s12223-015-0378-y   DOI
2 Delafont V, Rodier MH, Maisonneuve E, Cateau E. Vermamoeba vermiformis: a free-living amoeba of interest. Microb Ecol 2018;76: 991-1001. https://doi.org/10.1007/s00248-018-1199-8   DOI
3 Greub G, Raoult D. Microorganisms resistant to free-living amoebae. Clin Microbiol Rev 2004; 17: 413-433. https://doi.org/10.1128/CMR.17.2.413-433.2004   DOI
4 Lee DI, Park SH, Baek JH, Yoon JW, Jin SI, Han KE, Yu HS. Identification of free-living amoebas in tap water of buildings with storage tanks in Korea. Korean J Parasitol 2020; 58: 191-194. https://doi.org/10.3347/kjp.2020.58.2.191   DOI
5 Scheid PL, Lam TT, Sinsch U, Balczun C. Vermamoeba vermiformis as etiological agent of a painful ulcer close to the eye. Parasitol Res 2019; 118: 1999-2004. https://doi.org/10.1007/s00436-019-06312-y   DOI
6 Kim YK, Oh SY, Jeon SG, Park HW, Lee SY, Chun EY, Bang B, Lee HS, Oh MH, Kim YS, Kim JH, Gho YS, Cho SH, Min KU, Kim YY, Zhu Z. Airway exposure levels of lipopolysaccharide determine type 1 versus type 2 experimental asthma. J Immunol 2007; 178: 5375-5382. https://doi.org/10.4049/jimmunol.178.8.5375   DOI
7 Ockert G. Review article: occurrence, parasitism and pathogenetic potency of free-living amoeba. Appl Parasitol 1993; 34: 77-88 (in German).
8 Tanzifi A, Moghaddam Y, Dodangeh S, Daryani A, Sarvi S, Gholami S, Hosseini SA, Chegeni TN, Hosseininejad Z. Detection and molecular characterization of potentially pathogenic freeliving amoebae from recreational and public soils in Mazandaran, Northern Iran. Iran J Parasitol 2021; 16: 295-304. https://doi.org/10.18502/ijpa.v16i2.6316   DOI
9 Norouzi M, Saberi R, Niyyati M, Lorenzo-Morales J, Mirjalali H, Fatemi M, Javanmard E, Karamati SA. Molecular identification of pathogenic free-living amoeba from household biofilm samples in Iran: a risk factor for Acanthamoeba keratitis. Microorganisms 2021; 9: 2098. https://doi.org/10.3390/microorganisms9102098   DOI
10 Alizadeh H, Neelam S, Hurt M, Niederkorn JY. Role of contact lens wear, bacterial flora, and mannose-induced pathogenic protease in the pathogenesis of amoebic keratitis. Infect Immun 2005; 73: 1061-1068. https://doi.org/10.1128/IAI.73.2.1061-1068.2005   DOI
11 Ficker LA, Kirkness C, Wright P. Prognosis for keratoplasty in Acanthamoeba keratitis. Ophthalmology 1993; 100: 105-110. https://doi.org/10.1016/s0161-6420(93)31707-0   DOI
12 Schuster FL, Visvesvara GS. Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. Int J Parasitol 2004; 34: 1001-1027. https://doi.org/10.1016/j.ijpara.2004.06.004   DOI
13 Park MK, Cho MK, Kang SA, Park HK, Kim DH, Yu HS. Acanthamoeba protease activity promotes allergic airway inflammation via protease-activated receptor 2. PLoS One 2014; 9: e92726. https://doi.org/10.1371/journal.pone.0092726   DOI
14 Lee SJ, Jeong HJ, Lee JE, Lee JS, Xuan YH, Kong HH, Chung DI, Ock MS, Yu HS. Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear. Korean J Parasitol 2006; 44: 313-320. https://doi.org/10.3347/kjp.2006.44.4.313   DOI
15 Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin Microbiol Rev 2003; 16: 273-307. https://doi.org/10.1128/cmr.16.2.273-307.2003   DOI
16 Visvesvara GS, Moura H, Schuster FL. Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunol Med Microbiol 2007; 50: 1-26. https://doi.org/10.1111/j.1574-695X.2007.00232.x   DOI
17 Finn PW, Bigby TD. Innate immunity and asthma. Proc Am Thorac Soc 2009; 6: 260-265. https://doi.org/10.1513/pats.200807-064RM   DOI
18 Jeong HJ, Lee SJ, Kim JH, Xuan YH, Lee KH, Park SK, Choi SH, Chung DI, Kong HH, Ock MS, Yu HS. Acanthamoeba: keratopathogenicity of isolates from domestic tap water in Korea. Exp Parasitol 2007; 117: 357-367. https://doi.org/10.1016/j.exppara.2007.05.003   DOI
19 Song SM, Kang SA, Park HK, Kim DH, Park SY, Jang SB, Yu HS. Acanthamoeba profilin elicits allergic airway inflammation in mice. PLoS Negl Trop Dis 2018; 12: e0006979. https://doi.org/10.1371/journal.pntd.0006979   DOI
20 Park HK, Park MK, Kim KU, Kang SA, Park SK, Ahn SC, Kim DH, Yu HS. Evaluation of allergic sensitivity to Acanthamoeba allergen in patients with chronic cough. Allergy Asthma Proc 2016; 37: 141-147. https://doi.org/10.2500/aap.2016.37.3922   DOI
21 Yu HS, Angkasekwinai P, Chang SH, Chung Y, Dong C. Protease allergens induce the expression of IL-25 via Erk and p38 MAPK pathway. J Korean Med Sci 2010; 25: 829-834. https://doi.org/10.3346/jkms.2010.25.6.829   DOI
22 McBrien CN, Menzies-Gow A. The biology of eosinophils and their role in asthma. Front Med-Lausanne 2017; 4. https://doi.org/10.3389/fmed.2017.00093   DOI
23 Kim HY, DeKruyff RH, Umetsu DT. The many paths to asthma: phenotype shaped by innate and adaptive immunity. Nat Immunol 2010; 11: 577-584. https://doi.org/10.1038/ni.1892   DOI
24 Tung MC, Hsu BM, Tao CW, Lin WC, Tsai HF, Ji DD, Shen SM, Chen JS, Shih FC, Huang YL. Identification and significance of Naegleria fowleri isolated from the hot spring which related to the first primary amebic meningoencephalitis (PAM) patient in Taiwan. Int J Parasitol 2013; 43: 691-696. https://doi.org/10.1016/j.ijpara.2013.01.012   DOI
25 Clarke DW, Niederkorn JY. The pathophysiology of Acanthamoeba keratitis. Trends Parasitol. 2006; 22: 175-180. https://doiorg/10.1016/j.pt.2006.02.004   DOI
26 Beasley R, The Internationa Study of Asthma and ISAAC Steering Committe. Worldwide variation in prevalence of symptoms of asthma, allergic rhinoconjunctivitis, and atopic eczema: ISAAC. Lancet 1998; 351: 1225-1232. https://doi.org/10.1016/S0140-6736(97)07302-9   DOI
27 Garcia A, Goni P, Cieloszyk J, Fernandez MT, Calvo-Begueria L, Rubio E, Fillat MF, Peleato ML, Clavel A. Identification of freeliving amoebae and amoeba-associated bacteria from reservoirs and water treatment plants by molecular techniques. Environ Sci Technol 2013; 47: 3132-3140. https://doi.org/10.1021/es400160k   DOI
28 Yu HS, Kong HH, Kim SY, Hahn YH, Hahn TW, Chung DI. Laboratory investigation of Acanthamoeba lugdunensis from patients with keratitis. Invest Ophthalmol Vis Sci 2004; 45: 1418-1426. https://doi.org/10.1167/iovs.03-0433   DOI
29 O'Connell EJ, Rojas AR, Sachs MI. Cough-type asthma: a review. Ann Allergy 1991; 66: 278-282, 285.
30 Imai T, Nagira M, Takagi S, Kakizaki M, Nishimura M, Wang J, Gray PW, Matsushima K, Yoshie O. Selective recruitment of CCR4-bearing Th2 cells toward antigen-presenting cells by the CC chemokines thymus and activation-regulated chemokine and macrophage-derived chemokine. Int Immunol 1999; 11: 81-88. https://doi.org/10.1093/intimm/11.1.81   DOI
31 Robinson DS, Hamid Q, Ying S, Tsicopoulos A, Barkans J, Bentley AM, Corrigan C, Durham SR, Kay AB. Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma. N Engl J Med 1992; 326: 298-304. https://doi.org/10.1056/NEJM199201303260504   DOI
32 Calhoun WJ, Sedgwick J, Busse WW. The role of eosinophils in the pathophysiology of asthma. Ann N Y Acad Sci 1991; 629: 62-72. https://doi.org/10.1111/j.1749-6632.1991.tb37961.x   DOI
33 Fahy JV. Type 2 inflammation in asthma-present in most, absent in many. Nat Rev Immunol 2015; 15: 57-65. https://doi.org/10.1038/nri3786   DOI
34 Trabelsi H, Dendana F, Sellami A, Sellami H, Cheikhrouhou F, Neji S, Makni F, Ayadi A. Pathogenic free-living amoebae: epidemiology and clinical review. Pathol Biol (Paris) 2012; 60: 399-405. https://doi.org/10.1016/j.patbio.2012.03.002   DOI
35 Yu HS. Free-living amoeba Acanthamoeba triggers allergic inflammation of airways. In Pereira C ed, Allergic Diseases: New Insight. IntechOpen. Rijeka, Croatia. 2015. https://dx.doi.org/10.5772/59190   DOI
36 Potgieter N, van der Loo C, Barnard TG. Co-existence of free-living amoebae and potential human pathogenic bacteria isolated from rural household water storage containers. Biology (Basel) 2021; 10: 1228. https://doi.org/10.3390/biology10121228   DOI