• Title/Summary/Keyword: BAG3

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Carbon Dioxide Fixation using Spirulina Platensis NIES 39 in Polyethylene Bag (Spirulina Platensis NIES 39를 이용한 Polyethylene Bag 반응기에서의 이산화탄소 고정화)

  • Kim, Young-Min;Kim, Ji-Youn;Lee, Sung-Mok;Ha, Jong-Myung;Kwon, Tae-Ho;Lee, Jae-Hwa
    • Applied Chemistry for Engineering
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    • v.21 no.3
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    • pp.272-277
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    • 2010
  • To replace current expensive photobioreactor, this study was conducted to develop low-cost photobioreactor made of polyethylene bag. In previous study, optimal culture conditions of Spirulina platensis NIES 39 have been established, and based on these, the study of biological carbon dioxide fixation has been conducted. The maximum growth was the biomass 2.677 g/L at conditions of 10% $CO_2$, 0.1 vvm. It was shown that $F_{CO_2}$ was 4.056 g $CO_2$/L and $R_{CO_2}$ was 0.312 g $CO_2$/L/day. But, compared with the data at conditions of 5% $CO_2$, 0.1 vvm, $FE_{CO_2}$ was shown 52.372% which is half of it. Regarding the effect of $CO_2$ following illumination, the growth revealed that the input conditions, for 10 min per 3 h, were excellent in the light. $CO_2$ in absent light. $CO_2$ concentration and flow rate were 5% $CO_2$, 0.1 vvm, respectively. Finally, the addition of $CO_2$ was ineffective in the absence of light.

Evaluation of Mulberry (Morus alba) as Potential Feed Supplement for Ruminants: The Effect of Plant Maturity on In situ Disappearance and In vitro Intestinal Digestibility of Plant Fractions

  • Saddul, D.;Jelan, Z.A.;Liang, J.B.;Halim, R.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.11
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    • pp.1569-1574
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    • 2005
  • The in situ nylon bag degradation and in vitro intestinal digestibility of dry matter (DM), and crude protein (CP) of mulberry (Morus alba) plant fractions was studied at four harvest stages, 3 (W3), 5 (W5), 7 (W7) and 9 (W9) weeks. Degradability of DM and CP of the whole plant and stem fractions declined significantly (p<0.01) with advancing plant maturity in the order W3>W5 and W7>W9 and W3>W5>W7>W9, respectively. The degradation of DM and CP of the leaf fraction was also influenced by plant maturity but no trend was observed. The degradation of DM and CP of the whole plant and leaves increased rapidly during the first 48 and 24 h of incubation, respectively, when maximum degradation was reached. In vitro intestinal digestibility of CP was more influenced by the residence time in the rumen than by plant maturity. This study showed that mulberry is suitable as a supplement, particularly to low-quality roughages, in providing a source of rapidly available nitrogen to the rumen microbes, hence improving the roughage degradability and intake.

Development of fillet-type seasoner and mixed powder-type seasoner using smoked mackerel (훈제 고등어를 이용한 필렛형 조미료 및 복합 분말조미료의 개발)

  • 오영주;오혁수
    • Culinary science and hospitality research
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    • v.7 no.3
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    • pp.145-161
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    • 2001
  • In odor to develop fillet-type seasoner and mixed powder-type seasoner using smoked mackerel as primary material, processing conditions of the mackerel meat and flavoring extract from various materials were examined. The approximate composition of mackerel meat and mixed powde-type seasoner using mackerel as primary material were 10.9 and 11.7%, for moisture 79.1 and 57.2% for crude protein, 5.3 and 2.9% for crude lipid, and 4.3 and 24.6% for crude ash, respectively. It was suitable to simmer raw mackerel in 3% chitooligosaccaride solution at 8$0^{\circ}C$ for 10 min., for the enhancement of reaction their meat and chitooligosaccharide. Considering effect of type of woodsmoke on organoletic properties of mackerel after smoking, oak and mixture(oak wood + citrus wood = 1 : 1) were superior to another woodsmoke, the smoking conditions favoring the most overall preference were 8$0^{\circ}C$, 25min. for smoking time, 20$0^{\circ}C$, 20min. for baking, and 10$0^{\circ}C$. 4hrs. for drying. The optimal material mixing ratio for mixed powder-type seasoner was powder of smoked mackerel 60%, dried anchovy 15%, dried sea tangle 10%. The most appropriate extraction condition of mixed powder seasoner with packing tea bag(10g/bag) was 98$^{\circ}C$ for 5min. in the boiling water.

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A Study on the Effect of Cold Application Using a Sponge Bath in Healthy Adults (냉요법 적용방법에 따른 냉요법 효과에 관한 연구-건강한 성인 여성에서 스폰지 목욕방법을 중심으로)

  • Chung, Hyun-Sook;Kang, Kyu-Sook;Hwang, Ae-Ran
    • The Korean Nurse
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    • v.28 no.3
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    • pp.68-82
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    • 1989
  • This study was a quasi-experimental research study to test the characteristics of temperature regulation according to sponge bath methods of cold application. Thirteen volunteers were selected from among nursing college students according to an established criteria using a purposive sampling technique. Four different cold application methods were used: $\circled1$ tepid water sponge bath at $28^{\circ}C$, $\circled2$ 20% alcohol sponge bath at $28^{\circ}C$, $\circled3$ 40% alcohol sponge bath at $28^{\circ}C$ and $\circled4$ tepid water sponge bath at 28$^{\circ}$C plus an ice bag to the head. Changes in rectal temperature, mean skin temperature, mean body temperature, heat content change and thermal discomfort during the cold application were measured at 5 minute intervals over a 120 minute period. The data collection period was from Dec. 20, 1988 to Feb. 3, 1989. The data were analyzed using descriptive statistics, simple regression, ANOVA, Duncan's multiple range test and Pearson correlation coefficient using the SPSS-X Program. The results of the study are summarized as follows. Five general hypothesis were tested. Hypothesis 1 that "Change in heat content will be decreased for each cold application method according to the cold application time" was rejected. (tepid water sponge bath: after 10 minutes of cold application, 20% alcohol sponge bath: after 25 minutes of cold application: 40% alcohol sponge bath: after 45 minutes of cold application, tepid water sponge bath plus an ice bag to the head: after 80 minutes of cold application) Hypothesis 2 that "Thermal discomfort will be changed for each cold application method according to the cold application time" was rejected after 5minutes of cold application. Hypothesis 3 that "Change in heat content will differ among the cold application methods" was accepted except 0~5, 0~10, 0~65, 0~105 and 0~120 minute. This difference showed significance only between sponge bath methods and tepid water sponge bath plus an ice bag to the head. Hypothesis 4 that "Thermal discomfort will differ among the cold application methods" was accepted at 15, 20, 35, 45, 75, 80, 90, 95, 100, 105, 110, 115 and 120 minute of cold application time. This difference showed significance only between sponge bath methods and tepid water sponge bath plus an ice bag to the head. Hypothesis 5 that "The higher the change in heat content, the higher the thermal discomfort during the cold application time" was accepted for between 10~60 and 75 minute of cold application. In conclusion, this study showed that in sponge bath at $28^{\circ}C$, 10~80 minute was a effective cold application time in the view of heat loss through the skin. Concerning the effects of evaporation and thermal discomfort, it was found that there was no difference with regard to the solutions; tepid water sponge bath; 20% alcohol sponge bath or 40% alcohol sponge bath at a $28^{\circ}C$ controlled solution temperature. So it was thought that the type of solution itself did not have a big influence on the heat loss through skin. The combined effect of sponge bath with an ice bag to the head showed a significant difference and also showed a slight increase in thermal discomfort. On the basis of this research it can be concluded that cold application, for example, an ice bag to the head during a tepid water sponge bath is a good method as it increase heat loss through conduction, although fit can also cause a slight increase in thermal discomfort. The correlation between changes in heat content and thermal discomfort were not high. So factors other than change in heat content are considered to have an effect on the cognition of thermal discomfort.

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Effects of educational intervention on single-rescuer respiratory-assistant therapy using a bag valve mask (백-밸브-마스크를 이용한 1인 호흡보조요법 교육의 효과)

  • Lee, Yong-Jae;Uhm, Dong-Choon
    • The Korean Journal of Emergency Medical Services
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    • v.23 no.3
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    • pp.29-40
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    • 2019
  • Purpose: This study was conducted to identify the effects of educational intervention on pre-test and post-test tidal volume, endotracheal peak pressure, and ventilation interval measurements during single-rescuer respiratory-assistant therapy by paramedic students. Methods: The present study, with a quasi-experimental design, included a pre-test and post-test nonequivalent control group. A total of 62 paramedic students (31, experimental group; 31, control group) participated in this study. The intervention lasted 80 minutes. Data were collected from each student before the intervention and two weeks after the intervention, between September 3 and 21, 2018. The collected data were analyzed using IBM SPSS Statistics for Windows, Version 25.0. Results: Tidal volume (p<.001) and endotracheal peak pressure (p=.002) measurements after the intervention were significantly different between the two groups. Analysis of covariance was used to control the variance (the pretest value of endotracheal peak pressure) in order to identify the effect of the intervention in the two groups. Endotracheal peak pressure was not significantly different between the two groups. Conclusion: Education and training of paramedic students in emergency medical services on single-rescuer respiratory-assistant therapy is necessary for the emergency care of patients with respiratory arrest.

The evaluation of effectiveness of 3DCA using vacuum bag(Vac-Lock) for patient immobilization device (3DCA제작을 통한 Vac-Lock 사용시 효율성향상에 대한 연구)

  • Lee YoungChul;Lee ChulBin;Kang NoHyun;Kim DongEuk;Lee JungYong;Jeong InPyo
    • The Journal of Korean Society for Radiation Therapy
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    • v.16 no.1
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    • pp.67-72
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    • 2004
  • Purpose : Patient immobilization is crucial factor for radiation therapy. Generally, we have been used vacuum bag immobilization device(Vac-Lock) for whole body immobilization. In order to easily set up of vacuum bag(Vac-Lock), we made a 3DCA(3-Dimensional Conformal Accessary). The purpose of this study is evaluation of effectiveness of 3DCA using Vack-Lock for patient immobilization. Materials and Methods : We made 3DCA(3-Dimensional Conformal Accessary) tool of wooden boards. The reasons to choice of wooden boards are its easily handling nature and cheap expenses. Results : (1) We reduced man power from $5{\sim}6$persons to 1person to make immobilizations, (2) Shortened work time from 1hour to within 10minutes. (3) Avoid a collision to treatment gantry head. (4) Its shapes are smart and clean. Conclusion : We have made and used 3DCA(3-Dimensional Conformal Accessary) tool was very effective and convenience for the patients and users.

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Shape of the model pound net affected by wave and fish behavior to the net - Shape and tension of the model pound net affected by wave - (파동에 의한 모형정치망의 형상변화와 어류대망행동 - 파동에 의한 모형정치망의 형상과 장력변화 -)

  • Lee, Ju-Hee;Kwon, Byeong-Guk;Yun, Il-Bu;Kim, Sam-Kon;Yoo, Je-Bum;Kim, Boo-Young;Kim, Byung-Soo;Lee, Hye-Ok
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.43 no.2
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    • pp.101-115
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    • 2007
  • The pound net fishery is very important one in Korean coastal fishery and it need to grasp the characteristics of the net affected by many factors. It is considered that the structure and the shape of the pound net can be changed by the direction and speed of current, wave height, depth and conditions of sea bed. However, most of all, the speed of current and wave height influence more upon the pound net than any other factors to deform and flutter. In this study, author carried out the experiments with a model of double one-side pound net made by the similarity law as 1:100 scales at a real experimental area, and additionally the model net experiments were conducted in the circulating water channel in Pukyong National University. The author analyzed the data of transformation of shape and tension of the model pound net to recognize the characteristics of the current and wave acting on it. Regardless of the direction of flow affecting on the fish court net or bag net, the deformed angle and depth to the side panel and bottom of box nets becomes bigger as the wave gets higher and the period of wave is faster. The tension in both upward or downward tends to be changed by the speed of wave. Those value of changes occurred similarly in either fish court net or bag net. Generally, when bag net is located at upward of flow, the value of tension was bigger 10% than any other location or nets. Regardless of the setting direction, the tension of the pound net is increased in proportion to flow speed, wave height and period of wave, and it becomes bigger about 15-30% at upward to flow than downward. Where the flow is upward in the court net, the tension in the wave increased to 37% compared to the one in the flow only in the condition of flow of 0.1-0.3m/s. Where the flow is upward in the bag net, the tension in the wave increased to 52% in the flow of 0.1m/s, and the tension increased to 48% in the flow of 0.2-0.3m/s.

Studies on the Preservation of Korean Rice by Gamma-irradiation(II) On disinfestation of rice by gamma-ray irradiation (감마선 조사에 의한 쌀 저장에 관한 연구 (제 2 보))

  • Kim, Hyong-Soo;Choi, Young-Rack;Kim, Sung-Kih;Harn, In-Ja
    • Korean Journal of Food Science and Technology
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    • v.2 no.1
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    • pp.104-112
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    • 1970
  • Korean Paldal variety rice, harvested November and hulled into polished and unpolished forms in April, 1969, packed in the straw bags and the kraft paper bags. Being irradiated with 10, 30 and 50 krad ${\gamma}-ray$, the samples were stored at room temperature for testing infestation, uric acid content, fat acidity, riboflavin content and panel test. The results are as follows. 1. The kraft bag lot with polished rice or unpolished rice manifested the apparent disinfestation with $30{\sim}50$ krad irradiation. On the other hand, in the straw bag lot the irradiation effects on disinfestation were weakened through summer on account of the second contamination. 2. The changes of uric acid content in rice were proportional to the state of infestation. 3. Fat acidity, which has a close relation with the rancidity of fat in rice, increased during storage and showed some increasing tendency in the irradiated lot with $30{\sim}50$ krad. 4. In comparison with the control lot, riboflavin content was not affected by 50 krad irradiation. 5. With 10, 30 and 50 krad dose, the irradiated lot should have no significant difference in taste, odour, color and viscosity for the panel test.

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Real-Time RT-PCR on SAG1 and BAG1 Gene Expression during Stage Conversion in Immunosuppressed Mice Infected with Toxoplasma gondii Tehran Strain

  • Selseleh, Monavar;Modarressi, Mohammad Hossein;Mohebali, Mehdi;Shojaee, Saeedeh;Eshragian, Mohammad Reza;Selseleh, Mina;Azizi, Ebrahim;Keshavarz, Hossein
    • Parasites, Hosts and Diseases
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    • v.50 no.3
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    • pp.199-205
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    • 2012
  • Toxoplasmic encephalitis is caused by reactivation of bradyzoites to rapidly dividing tachyzoites of the apicomplexan parasite Toxoplasma gondii in immunocompromised hosts. Diagnosis of this life-threatening disease is problematic, because it is difficult to discriminate between these 2 stages. Toxoplasma PCR assays using gDNA as a template have been unable to discriminate between an increase or decrease in SAG1 and BAG1 expression between the active tachyzoite stage and the latent bradyzoite stage. In the present study, real-time RT-PCR assay was used to detect the expression of bradyzoite (BAG1)- and tachyzoite-specific genes (SAG1) during bradyzoite/tachyzoite stage conversion in mice infected with T. gondii Tehran strain after dexamethasone sodium phosphate (DXM) administration. The conversion reaction was observed in the lungs and brain tissues of experimental mice, indicated by SAG1 expression at day 6 after DXM administration, and continued until day 14. Bradyzoites were also detected in both organs throughout the study; however, it decreased at day 14 significantly. It is suggested that during the reactivation period, bradyzoites not only escape from the cysts and reinvade neighboring cells as tachyzoites, but also converted to new bradyzoites. In summary, the real-time RT-PCR assay provided a reliable, fast, and quantitative way of detecting T. gondii reactivation in an animal model. Thus, this method may be useful for diagnosing stage conversion in clinical specimens of immunocompromised patients (HIV or transplant patients) for early identification of tachyzoite-bradyzoite stage conversion.

Suitable substrate formulation for bag cultivation of the cultivar 'Haesal' in Hypsizygus marmoreus (느티만가닥버섯 '햇살' 품종 봉지재배 적합배지 조성 연구)

  • Kim, Min-Keun;Sim, Soon-Ae;Kim, Ah-Young;Kwon, Jin-Hyeuk;Chang, Young-Ho
    • Journal of Mushroom
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    • v.19 no.3
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    • pp.160-165
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    • 2021
  • This study was conducted to investigate a suitable substrate formulation for bag cultivation of Hypsizygus marmoreus. To determine the optimum media composition for H. marmoreus, chemical properties of various media and culture properties were investigated. The fastest primordia formation (9.0 days), the shortest fruiting body harvest period (18.0 days), and the highest yield (144.7 g/bag) were realized with the T6 formulation (55:15:20:5:5 mixing ratio of poplar sawdust, wheat bran, rice bran, dried soybean powder, and palm kernel, respectively). The chemical properties of the T6 formulation were pH, 6.0; total carbon, 19.0%; total nitrogen, 0.83%; C/N ratio, 22.8; P2O5, 0.61%; K2O, 0.46%; and MgO, 0.29%. This is the first attempt using plastic bags for cultivation and will help expand the production of H. marmoreus.