• KSBB Journal
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• v.9 no.2
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• pp.115-121
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• 1994

A Purification device with 30-channel free-flow electrophoresis was assembled to treat samples of 240m1 volume for purification of lentil lectin (LcH) from lentil seeds with no impurities in a silverstained PAGIEF gel. HEPES(50mM)-Ttis(50mM), Cycloserine(50mM)-urea(3M), Histidine(50mM)-urea(3M) were used as ampholytea among which Histidine(50mM)-urea(3M) (pI 7.65) was found best in resolution. LcH is known to be present in the form of LcH-A, LcH-B and the complex of the two. The complex, however, disappeared when urea was added in the ampholytes, which suggested that the complete purification of two isolectins is possible using the present multistep purificaton device.

• Journal of the Korean Chemical Society
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• v.30 no.1
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• pp.109-117
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• 1986

7,9-Dideoxydaunomycinone (32), a late-stage precursor of the aglycone of antitumor antibiotic daunorubicin(2a) was prepared from 3-methoxybenzoic acid(5). Thus, 3-methoxybenzoic acid was converted to 4-methoxy-3-(phenylsulfonyl)-1(3H)-isobenzofuranone(11), which furnished trimethoxynaphthoate 16 upon ring annelation developed by Hauser and Rhee. The trimethoxynaphthoate 16 upon ring annelation developed by Hauser and Rbee. The trimethoxynaphtboate 16 was then transformed into phenylsulfonylnaphthofuranone 22, which was used to make anthracenoate 24 via Michael type reaction with 7-(ethylenedioxy)-2-octenoate(23). Conversion of anthracenoate 24 to tetracyclic product 28, followed by subsequent deprotection of the methyl groups in ring-B and C furnished 7, 9-Dideoxydaunomycinone(32).

• Advances in Traditional Medicine
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• v.10 no.4
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• pp.319-321
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• 2010

Plant saponins are widely distributed amongst plants and have a wide range of biological properties. Icosahydropicenic acid, $C_{51}H_{80}O_{19}$ ((4S,6bS)-8a-((4,5-dihydroxy-6-methyl-3-((3R)-3,4,5-trihydroxy-6-methyl-tetrahydro-2H-pyran-2-yloxy)-tetrahydro-2H-pyran-2-yloxy)carbonyl)-2-hydroxy-4, 6a, 6b, 11, 14b-pentamethyl-11-(2-methylprop-1-enyl)-3-(3,4,5-trihydroxy-6-(hydroxymethyl) - tetrahydro-2Hpyran-2-yloxy)-1, 2, 3, 4, 4a, 5, 6, 6a, 6b, 7, 8, 8a, 9, 10, 11, 12, 12a, 14, 14a, 14b-icosahydropicene-4-carboxylic acid), a new saponin was first time isolated from the roots of Clerodendrum Serratum (L) Moon (Verbenaceae). The structure elucidation of the compound was carried out by $^1H$ NMR and DART-MS studies.

• Journal of Microbiology and Biotechnology
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• v.19 no.9
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• pp.1013-1018
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• 2009

The identification and characterization of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) was conducted using TEM, an API 50CHB kit, 16S rDNA sequencing, a phylogenetic tree, and catalase and oxidase testing. The conversion rate of glucose to lactic acid by B. polyfermenticus KJS-2 was found to be $60.7{\pm}4.9%$. In addition, treatment of B. polyfermenticus KJS-2 with artificial gastric juice (pH 2.0) and bile acid (pH 6.5) for 4 h resulted in a final viability of $140{\pm}7.9%$ and $108{\pm}3.5%$, respectively. Finally, the results of adhesion experiments using Caco-2 cells revealed that the adherence of B. polyfermenticus KJS-2 to Caco-2 cells was approximately $65{\pm}0.6%$.

• Polymer(Korea)
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• v.35 no.4
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• pp.308-313
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• 2011

The ring-opening isomerization polymerization behavior of 2,5,6,7-tetrahydro-3H-imidazo[2,1-b] [1,3] oxazine(TII) has been studied under various conditions. The present study aimed at improving the low polymerizability of the above monomer (six-membered ring) up to a level comparable to that of 2,5,6-tetrahydroimidazo[2,1-b] [l,3] oxazole(TIO) (five-membered ring). The optimum result could be observed when methyl trifluoromethanesulfonate(MeOTf) and nitrobenzene were used as an initiator and a solvent in the polymerization at $60^{\circ}C$ for 24 h. Polymers were confirmed by$^1H$ NMR과$^{13}C$ NMR, and m measurements. The molecular weight obtained by gel permeation chromatography (GPC) corresponded to the molecular weight theoretically calculated from the feed ratio. The melting temperature ($T_m$) of TII was conspicuously different from that of TIO, because of the difference in the alkyl group.

• Korean journal of applied entomology
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• v.28 no.3
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• pp.167-174
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• 1989

A confined field assessment on the integrated control of vector mosquitoes (Anopheles sinen- sis and Culex tritaent'orhynchus) was conducted by timely treatment of Bacillus thuringiensis(H-14) formulation in the presence of larvivorous fish (Aplocheilus lalipes) in natural rice fields of $30,000M^2$ at Yongam, South Korea Cholla Province from July through October in 1988. In presence of larvivorous fish (Aplocheilus) at lower density of 0.6 fish per $M^2$ water surface, mosquito vector control rates ranged 55. 0~57. 6% from July through August, and when the mosquio density started to increase with over 10 larvae on the average per sample, B.t.(H-14) formulation treatment at the rate of 1. 0 Kg/ha made 100% vectors reduc¬tion in 24 hours, the control sustained above 98% until the test was terminated on October 11th. In the fish introduced rice paddies with Aphyocypris chinensis at the density of above 1. 5 fish per $M^2$, a satisfactory degree of vector control was obtained by 88. 2~96. 7% in 2 week period until September 21st. In the absence of larvivorous fishes, B.t. (H-14) treatment made 100% control in 24 hours, however, vector population rebound appeared in day 7, required to make additional treatment to suppress population down.

• Bulletin of the Korean Chemical Society
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• v.16 no.9
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• pp.835-839
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• 1995

Mer,trans-Re(≡NC6H5)(PPh3)2Cl3, Ⅰ, reacted with trimethylphosphine to give a mixture of two stereoisomers, mer,trans-Re(≡NC6H5)(PMe3)2Cl3,Ⅱ, and fac,cis-Re(≡NC6H5)(PMe3)2Cl3, Ⅲ. These compounds could also be prepared from the reaction of Re(≡NC6H5)(PMe3)(PPh3)Cl3 with trimethylphosphine. In both reactions the mer,trans-isomer is a major product. The products have been characterized by NMR, elemental analysis, and X-ray crystallography. Crystal data for Ⅱ: monoclinic space group P21, a=10.053(1) Å, b=10.844(1) Å, c=10.058(2) Å, β=113.45(2)°, Z=2, R(wR2)=0.0348 (0.0894). Crystal data for Ⅲ: monoclinic space group P21/n, a=7.183(2) Å, b=16.983(4) Å, c=15.543(4) Å, β=90.38(2)°, Z=4, R(wR2)=0.0603 (0.1484).

• Applied Biological Chemistry
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• v.7
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• pp.45-52
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• 1966

This study was carried out to determine how the water soluble, the available $P_2O_5$ and urea-N would change in the course of time, when the mixtures of calcium superphosphate and urea with lime for fertilizer which occurred in Korea and largely contained calcium carbonate were made. Three kinds of materials, i. e., calcium superphosphate, urea and lime for fertilizer were used in this study. Three kinds of mixed fertilizer, i. e., A, B and C were made up by mixing these materials to satisfy the following formula. $$1)\;Ca(H_2PO_4)_2+CaCO_3+CO(NH_2)_2{\rightarrow}$$$$Ca_2H_2(PO_4)+H_2CO_3+NH_3$$ $$2)\;Ca(H_2PO_4)_2+CaCO_3+CO(NH_2)_2{\rightarrow}$$$$Ca_3(PO_4)_2+H_2CO_3+NH_3$$ $$3)\;Ca(H_2PO_4)_2+CaCO_3+CO(NH_2)_2{\rightarrow}$$$$Ca_3(PO_4)_2+H_2CO_3+CaCO_3+NH_3$$ A,B and C were placed in desiccators respectively a six month period. During the time of storage, the water soluble, the available phosphoric acid and urea-N were measured once a month, seven times with the control measurement. The results may be summarized as follows. 1. None of A, B and C showed any change in the urea-N with the lapse of time. This fact indicated that the combination of calcium superphosphate and urea with lime for fertilizer was not unfavourable. 2. A, B and C decreased in the amount of water soluble $P_2O_5$ with the passage of time. This fact indicated that the mixing of calcium superphosphate and urea with lime for fertilizer was unfeasible. 3. The available $P_2O_5$ in any of A,B and C did not undergo a change as time went by. This fact suggested that the combination of calcium superphate and urea with lime for fertilizer was favourable.

• Korean Journal of Animal Reproduction
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• v.9 no.2
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• pp.140-147
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• 1985

Seventy four Holstein heifers were randomly assigned over three trials to PRID-7+PG-6 and Synchromate B-9 regimens to synchronize estrus cycle for embryo transfer. Sexual behaviors; moounting, standing, orientation, chin-resting, sniffing, licking, rubbing and butting, vaginal swelling and mucus discharge were observed between 06-08, 12-14 and 18-20 h on 1st day and 00-02, 06-08, 12-14 and 18-20 h on 2nd day after removal of hormones. Synchromate-B treatment (81.6%) showed higher synchronized estrus rate than PRID treatment (77.8%) during observation period. Standing estrus was observed within 74 h after PG injection in PRID and within 52 h after removal of implant in Synchromate-B. About 68% of heifers in PRID and 74% of heifers in Synchromate-B showed standing estrus between 0-14 h on 2nd day after removal of the hormones. Synchromate-B resulted in a tighter synchrony of standing estrus than PRID. Incidence of average mounting and standing per head during observation period was 22.3 and 16.6 in PRID and 28.1 and 13.6 in Synchromate-B. The PRID showed peak in active mounting at 18-20 h on 1st day, however, the Synchromate-B showed at 0-2 h on 2nd day after removal of hormone. Active standing was shown between 18 h on 1st day to 20h on 2nd day in PRID, however, between 0-14 h on 2nd day after removal of hormone is Synchromate-B. There was slight difference in pattern of active mounting and standing during estrus between PRID and Synchromate-B. Conception rate of synchronized heifers transferred with fresh and frozen embryos by non-surgical and surgical methods was higher in synchromate-B (62.5%) than in PRID (38.5%). Chin-resting showed highest incidence among 6 sexual behavioral components in the both treatments. Synchromate-B showed higher incidence of chin-resting (16.6) than PRID (10.7). Synchromate-B group showed also higher incidence of orientation, sniffing and butting than PRID group. Synchromate-B resulted in more active sexual behaviors than PRID. The pattern of incidence of chin-resting, licking and butting was almost symmetrical in PRID with their peak values at 6-8 h on 2nd day, however in Synchromate-B chin-resting and sniffing was symmetrical with their peak values at 12-14 h on 2nd day after removal of hormone. There was tendency to increase vaginal swelling according to time passage of synchronized estrus in the both treatments. Incidence of mucus discharge in Synchromate-B was slightly higher than in PRID. Twenty to 40% was false negative in conception rate by tall painting before re-estrus day as judged by rectal palpation.

• Journal of Microbiology and Biotechnology
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• v.5 no.3
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• pp.149-153
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• 1995

A simple and low-cost medium was developed for the growth of Bifidobacterium longum KFRI 977. Of three bifidobacterial strains, B. longum KFRI 977 (ATCC 15707) showed the best growth in MRSC broth containing 0.3% oxgall, grew well in partially anaerobic condition, exhibited highest $\beta$-galactosidase activity, and was inhibitory against Clostridium perfringens KFRI 434. Of three developed media, the population of B. longum KFRI 977 was highest (1.9$\times$$10^9$/ml) in ISP based medium. The composition of ISP based medium is ISP (5%), glucose (1%), L-cysteine HCI (0.05%), Trypticase peptone (0.5%), yeast extract (0.5%), $MgSO_4$ (0.05%), Tween-80 (0.1%), and phosphate buffer (pH 7.0). Hydrolysis of ISP by Protease A was unnecessary, and the use of phosphate buffer (pH 7.0) prevented the formation of protein precipitate. Associative culture of B. longum KFRI 977 with Lactobacillus acidophilus KFRI 233 was proven to be deleterous to the growth of B. longum KFRI 977.