• Journal of the Korean housing association
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• v.11 no.4
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• pp.85-96
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• 2000

The purpose of this study was to evaluated noise environment of the low-rise multi-family house in Athens, Georgia. The results of this study can be applied to the prevention of noise when planning multi-family houses, roads and cities in Korea. The subjects of this study were the place of 1m, 10m, 50m, 100m from 4-lane road ofT apartment and 233 residents lived in the low-rise multi-family houses in Athens. The results of this study were as follows. 1. The average noise level at 1m, 10m, 50m and 100m was 55.5dB, 46.7dB, 43.0dB, 43.0dB and 41.2dB respectively from 4-lane road of T apartment. From the view of the standard in this study, the physical noise was good. 2. The results of the residents subjective response on the noise were as follows. The external noise residents felt - that was a noise by operating machines, and traffic noise - ware as 2.57 on average. The noise by the daily activities, the noise of nature are a little as 2.38 on average. They were disturbed a little by the external noise. They felt the solid born sounds(average 2.49) more than airbone sound(average 2.23) by the internal noise. They are suffered worse from noise in summer and they worse from 8-12 oclock due to external noise and 16-20 oclock due to internal noise. Comparing the noise environment of Korean with that of American, I found a meaningful difference of nature and the noise of daily activities of external noise and all internal noise.

• The Journal of Pediatrics of Korean Medicine
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• v.28 no.3
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• pp.47-58
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• 2014

Objectives GyejigaChulBuTang (GCBT) is a prescription used to treat acute and chronic arthritis in Korea, China, and Japan. This study assessed the anti-inflammatory and anti-oxidant activities of GCBT on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Methods Raw264.7 cells were pretreated with or without GCBT for 1 hour prior to incubation with LPS. Anti-inflammatory activity of GCBT was evaluated with reference to gene expression and production levels of proinflammatory cytokines ($TNF{\alpha}$, IL-$1{\beta}$, IL-6, GM-CSF and $INF{\gamma}$) and inflammatory mediators (iNOS, COX-2, NO and $PGE_2$). In addition, intracellular ROS generation and signal transduction of MAPK family, PI3K/Akt and $I{\kappa}B{\alpha}/NF{\kappa}B$ was investigated. Results Prior treatment with GCBT inhibited elevation of $TNF{\alpha}$, IL-$1{\beta}$, IL-6, GM-CSF, $INF{\gamma}$, NO and $PGE_2$, together with their cognate mRNAs in a dose-dependent manner. Intracellular ROS contents were similarly reduced. These effects were due to inhibition of LPS-induced phosphorylation of MAPK family, PI3K/Akt and $I{\kappa}B{\alpha}$ as well as nuclear translocation of $NF{\kappa}B$. Conclusions GCBT suppresses pro-inflammatory mediators. GCBT has potential in the treatment of juvenile rheumatoid arthritis associated with inflammation.

• Journal of Nutrition and Health
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• v.20 no.4
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• pp.258-271
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• 1987

This survey of 527 children, aged 4 to 6 years, focused on family and dietary environment, nutritional status and personality. It was conducted from June to October, 1986. The results showed that 68.0%(breakfast)and 55.4% (evening meal ) of the subjects indicated they had meals with the family everyday. Analysis of the nutritional status children showed all nutrient intake except protein an diron was insufficient compared with RDA. Carbohydrate provided 58.3% of total calorie intake ; protein accounted for 15.7% . fat provided 26.0%. There was a positive relation between income and nutrient intake. Children with employed mothers showed lower nutrient intake than children with unemployed mothers. Food attitude was positively related to nutrient intake, nutrient intake of children with good eating behavior was higher than that of children had poor eating behavior . Activity level had a significant correlation with intake of calories, fat, vitamin A, vitamin B$_1$, and Vitamin B$_2$. Mood was correlated with intake of protein, iron, vitamin A, vitamin B$_2$ and vitamin C.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1359-1367
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• 2005

The gene encoding Pyrobaculum arsenaticum DNA polymerase (Par DNA polymerase) was cloned and sequenced. The gene consists of 2,361 bp coding for a protein with 786 amino acid residues. The deduced amino acid sequence of Par DNA polymerase showed a high similarity to archaeal family B-type DNA polymerases (Group I), and contained all of the motifs conserved in the family B-type DNA polymerases for $3'{\rightarrow}5'$ exonuclease and polymerase activities. The Par DNA polymerase gene was expressed under the control of the T7lac promoter on the expression vector pET-22b(+) in Escherichia coli BL21-CodonPlus(DE3)-RP. The expressed enzyme was purified by heat treatment, and Cibacron blue 3GA and $Hirap^{TM}$ Heparin HP column chromatographies. The optimum pH of the purified enzyme was 7.5. The enzyme activity was activated by divalent cations, and was inhibited by EDTA and monovalent cations. The half-life of the enzyme at $95^{\circ}C$ was 6 h. Par DNA polymerase possessed associated $3'{\rightarrow}5'$ proofreading exonuclease activity, which is consistent with its deduced amino acid sequence. PCR experiment with Par DNA polymerase showed an amplified product, indicating that this enzyme might be useful in DNA amplification and PCR-based applications.

• Structural Engineering and Mechanics
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• v.7 no.6
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• pp.561-573
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• 1999

A $C^*$-convergence algorithm for finite element analysis has been proposed by Bigdeli and Kelly (1997) and elements for the first three levels applied to planar elasticity have been defined. The fourth level element for the new family is described in this paper and the rate of convergence for the $C^*$-convergence algorithm is investigated numerically. The new family adds derivatives of displacements as nodal variables and the number of nodes and elements can therefore be kept constant during refinement. A problem exists on interfaces where the derivatives are required to be discontinuous. This problem is addressed for curved boundaries and a procedure is suggested to resolve the excessive interelement continuity which occurs.

• BMB Reports
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• v.43 no.7
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• pp.485-490
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• 2010

Neurotrophins regulate many aspects of neuronal function through activation of the high affinity Trk receptors. Shc family proteins are implicated in the coupling of RTK to the Ras/mitogen-activated protein kinase signaling cascade. Here we report that the fourth Shc family member, ShcD, associates with TrkB receptor and regulates BDNF-induced MAPK activation. Yeast two-hybrid assay and Co-IP experiments demonstrate ShcD interacts with TrkB in a kinase-activity-dependent manner. Confocal analysis shows ShcD cololizes well with TrkB in transfected 293T cells. Subsequent mapping experiments and mutational analysis indicate that both PTB and SH2 domains are capable of binding to TrkB and PTB domain binds to TrkB NPQY motif. Furthermore, ShcD is involved in BDNF-induced MAPK activation. In summary, we demonstrate that ShcD is a substrate of TrkB and mediates TrkB downstream signaling pathway.

• Journal of Korean Critical Care Nursing
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• v.7 no.2
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• pp.58-67
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• 2014

Purpose: The purpose of this study was to investigate the nursing work environment and family satisfaction in Korean intensive care units (ICUs). Methods: The study participants were 190 critical care nurses and 133 family members of ICU patients who were randomly chosen from four of the hospitals located in B city. The Korean Nursing Work Environment Scale was used to assess the work environment of critical care nurses. Family satisfaction was measured with the Korean version of the Critical Care Family Needs Inventory. Results: Critical care nurses reported moderate satisfaction with their work environment. The mean score for family satisfaction was 3.59 on a 5-point scale, and satisfaction with information provision received the highest score. Family satisfaction was higher in hospitals where the critical care nurses evaluated their work environment positively. Conclusion: This study revealed that the work environment of nurses affects family satisfaction in ICUs. Therefore, it is necessary to explore various methods of improving the critical care nursing work environment in order to provide the highest possible level of nursing care.

• Journal of Fashion Business
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• v.18 no.4
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• pp.28-44
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• 2014

The purpose of this study is to analyze the hat fashion worn by the British royal family. The research range has been limited to hats which Elizabeth II, Camilla Parker Bowles, and Kate Middleton wore from April 29, 2011 to April 11, 2014 when Kate Middleton married Windsor Prince William and became part of the royal family. The methods of the research were: previous studies and literature about the royal family were referred to, the function and types of hats were considered, and then the plasticity of the designs seen in hat fashion of the royal family were analyzed. The results are as follows: first, the shape of the hats of the British royal family: Three-dimensional shapes(84.2%) were the most frequent, the detailed shape of the hat Canotier(33.5%), Boater(12.7%) and Bowler(10.3%) had greater frequency. Second, the color of the hats were W(13.7%), Bk(10.5%), Y(9.8%), B(9.6%), YR/PB(9.4%) and RP(9.2%) color. The Color Combination is the Monochrome Color(62.3%), Analogous Color(16.1%), Accent Color(13.2%) and Complementary Color(8.4%) were most frequent. Third, the material of the hats were Felt(44.5%) and Straw(40.3%) were used most frequently. Finally, the decoration of hats were: Flowers(25.7%), String(25.0%), Ribbon(18.4%) and Feathers(17.9%) were the most common. This study can be utilized as basic data for effective styling and design ideas in the field of total fashion.

• Animal cells and systems
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• v.5 no.2
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• pp.133-137
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• 2001

Long terminal repeats (LTRs) of the human endogenous retrovirus K family (HERV-K) have been found to be coexpressed with sequences of genes closely located nearby. We examined transcribed HERV-K LTR elements in human brain tissue. Using cDNA synthesized from mRNA of the human brain, we performed PCR amplification and identified ten HERV-K LTR elements. These LTR elements showed a high degree of sequence similarity (92.4-99.7%) with the human-specific LTR elements. A phylogenetic tree obtained by the neighbor-joining method revealed that HERV-K LTR elements could be divided into two groups through evolutionary divergence. Some HERV-K LTR elements (HKL-B7, HKL-B8, HKL-B10) belonging to the group II from human brain cDNA were closely related to the human-specific HERV-K LTR elements. Our data suggest that HERV-K LTR element are active in the human brain; they could conceivably play a pathogenic role in human diseases such as psychosis.

• Journal of Breast Cancer
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• v.21 no.4
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• pp.371-381
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• 2018

Purpose: Immune suppression is common in patients with advanced breast cancer but the mechanisms underlying this phenomenon have not been sufficiently studied. In this study, we aimed to identify B7 family members that were able to predict the immune status of patients, and which may serve as potential targets for the treatment of breast cancer. We also aimed to identify microRNAs that may regulate the expression of B7 family members. Methods: The Cancer Genome Atlas data from 1,092 patients with breast cancer, including gene expression, microRNA expression and survival data, were used for statistical and survival analyses. Polymerase chain reaction and Western blot were used to measure messenger RNA and protein expression, respectively. Luciferase assay was used to investigate direct microRNA target. Results: Bioinformatic analysis predicted that microRNA (miR)-93, miR-195, miR-497, and miR-340 are potential regulators of the immune evasion of breast cancer cells, and that they exert this function by targeting CD274, PDCD1LG2, and NCR3LG1. We chose CD274 for further investigations. We found that miR-195, miR-497, and CD274 expression levels were inversely correlated in MDA-MB-231 cells, and miR-195 and miR-497 expressions mimic inhibited CD274 expression in vitro. Mechanistic investigations demonstrated that miR-195 and miR-497 directly target CD274 3' untranslated region. Conclusion: Our data indicated that the level of B7 family members can predict the prognosis of breast cancer patients, and miR-195/miR-497 regulate CD274 expression in triple negative breast cancer. This regulation may further influence tumor progression and the immune tolerance mechanism in breast cancer and may be able to predict the effect of immunotherapy on patients.