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Notes on Five Unrecorded Endophytic Fungi Isolated from Coniferous Leaves and Orchid Roots in Korea (한국에 서식하는 침엽수의 잎과 난초과 식물의 뿌리에서 분리한 5종의 국내 미기록 내생균)

  • Park, Hyeok;Lee, Bong-Hyung;Bae, Yu-Ra;Kim, Dong-Yeo;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.365-370
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    • 2016
  • We collected leaves of Pinus koraiensis and Thuja koraiensis and roots of Bletilla striata from various sites in Korea. The leaf and root samples were surface-sterilized and endophytic fungi were isolated. Fungal isolates were identified based on their morphological characteristics and a phylogenetic analysis of internal transcribed spacer regions, large subunit regions, and the ${\beta}$-tubulin gene. Consequently, we identified five species of endophytic fungi, namely Colletotrichum simmondsii, Fusarium sterilihyphosum, Diatrypella pulvinata, Ochroconis globalis, and Sphaeria chrysosperma. These species have not been previously reported in Korea and we report them here with descriptions and illustrations.

Inhibition of Tumor Growth through Macrophage Activation by Polysaccharide Fraction from Peonia japonica (PJ-P) (백작약 조다당분획에 의한 대식세포 활성화를 통한 암세포 증식 억제)

  • 박혜란;정우희;정일윤;이성태;조성기
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.1
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    • pp.149-154
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    • 2003
  • The immunomodulatory activity of PJ-P, a polysaccharide fraction extracted from Paeonia japonica, were reported in our previous paper. In the present study, we investigated that PJ-P inhibited cancer growth through activation of macrophages. The activities of peritoneal macrophage to induce tumor necrosis factor (TNF)-$\alpha$, interleukin-1 (IL-1)$\beta$, interleukin-6 (IL-6) and interleukin-12 (IL-12) as well as to ingest fluorescence-latex microbeads were enhanced by treatment of PJ-P. Direct cytocidal activity of PJ-P against cancer cells was not shown. However, in vitro, peritoneal macrophages treated with PJ-P had an activity to kill cancer cells. Furthermore, PJ-P significantly prolonged the survival of mice implanted intraperitoneally with B16F0 mel-anoma cells. These results suggest that PJ-P could be a useful immunomodulator and assistant of anti-tumor agent.

Cytotoxicity and Antioxidant Activities of the Yeonkyo-san and Its ingredients (연교산과 구성약재에 대한 항암 및 항산화효과)

  • Lee, Jin-Tae;Lee, Chang-Eon;Son, Jun-Ho;Lee, Jin-Young;Park, Tae-Soon;Lee, In-Cheol;Cheon, Soon-Ju;Pyeon, Jeong-Ran;Jee, Seon-Young;Jo, Cheol-Hun;An, Bong-Jeun
    • The Korea Journal of Herbology
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    • v.20 no.3
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    • pp.19-27
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    • 2005
  • Objectives : The antioxidant activities of Yeonkyo-san and its ingredient were investigated for industrial application. Methods : We experimented radical scavenging effect, superoxide dismutase and cytotoxicity effect of various cancer cell. Results : The result were obtained as follows : Primary testing of cytotoxicity of Yeonkyo-san and ingredient was done for G361. B16F10, MDA and A549 cell lines. Yeonkyo-san and ingredient water extract, ethanol extract showed cytotoxicity of over 90% respectively against cell line. The electron donating ability(EDA) of water extract from the Yeonkyo-san was higher than 60%, ethanol extract from the Yeonkyo-san was over 80% at 1000 ppm. The electron donating ability(EDA) of water extract and ethanol extract from the Yeonkyo-san ingredients were increased as well. SOD-like activity was high as 75% in 700 ppm, it was increased. All the other samples showed less than 40% SOD-like activity. Conclusions : The results indicated that water extract and ethanol extract of Yeonkyo-san and ingredient can be used as a natural ingredient with biological functions in cosmetic and food composition.

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A Study on the Analysis of Patent Information in the Apparel Design -Focused on International Patent Classification- (의류디자인 분야의 특허정보 분석 -국제특허분류를 중심으로-)

  • 이금희
    • The Research Journal of the Costume Culture
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    • v.11 no.6
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    • pp.835-851
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    • 2003
  • This study analyses patent information of apparel design using computer technology and researches the trend of patent application focused on International Patent Classification. In terms of trend by filling data, Patent application started first in 1974 and increased sharply in 1993 with 14 cases and increased to 25 cases in 2000. In case of Korea, they began somewhat late in 1996, but reached a similar level with the leading country in 2000. In terms of trend by applicant, Gerber Garment Technology, Inc. filed 7 cases TORAY IND INC, filed 6 cases Levi Strauss & Co. filed 4 cases, NEC HOME ELECTRONICS LTD filed 3 cases, TOYOBO CO LTD filed 3 cases. Japanese companies occupied 52% and United States's companies occupied 48%. In terms of trend by country, foreigner occupied 47% of the patents filed by United State. Japanese take up 10% of total patent of United States. Korean occupied 84% of total patent of Korea and foreigner, american occupied 16% of the patents filed by Korea. In regared to International Patent Classification, in the section level G filed 92 cases(53%). In class level, G06 marked the first place in United States, Japan, and Korea. In subclass level, G06F marksed the first place with 74 cases. G06T and A61B were regarded as the new technologies. The new technologies are representing the dimensions of garment or computer-rendered model, providing the virtual reality through the texture mapping, digital dressing room or virtual dressing, and performing or retriving display on a screen for the result of changing pattern ao dress design, The technologies of core patent are designing or producing custom manufactured item, providing or prealtering the data for pattern making and visually displaying, interactively generating or previewing of various articles.

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Inhibitory Effect of the Ethanol Extract of Fagopyrum escuentum on Melanin Synthesis (교맥 에탄올 추출물의 멜라닌생성 억제효과)

  • Kim, Dae-Sung;No, Seong-Taek;Lee, Jang-Cheon;Lim, Kyu-Sang;Shin, Mee-Ran;Woo, Won-Hong;Mun, Yeun-Ja
    • The Journal of Traditional Korean Medicine
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    • v.15 no.1
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    • pp.70-76
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    • 2006
  • The aim of this study was to investigate the effect of ethanol extract of Fagopyrum escuentum(FE) on the melanogenesis. To determine whether ethanol extract of FE suppress melanin synthesis in cellular level, B16F10 melanoma cells were cultured in the presence of different concentrations of FE ethanol extract. In the present study, the author examined the effects of FE ethanol extract on cell proliferation, melanin contents, tyrosinase activity. Cell proliferation was slightly increased by treatment with ethanol extract of FE (25-200 ${\mu}$g/ml). The ethanol extract of FE effectively suppressed melanin contents at a dose of 100 ${\mu}$g/ml. It was observed that the color of cell pellets was totally whitened compared with the control. The ethanol extract of FE inhibited tyrosinase activity, regulate melanin biosynthesis as the key enzyme in melanogenesis. These results suggest that the ethanol extract of FE exerts its depigmenting effects through the suppression of tyrosinase activity. And it may be a potent depigmetation agent in hyperpigmentation condition.

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The Influence of Ego-resilience, Appearance Satisfaction and Career Motivation on Nursing Students' Job Seeking Stress (간호대학생의 자아탄력성, 외모만족도, 진로동기가 취업스트레스에 미치는 영향)

  • Choi, Sook-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.3
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    • pp.386-393
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    • 2019
  • This study was conducted to investigate the influence of ego-resilience, appearance satisfaction and career motivation on nursing students' job seeking stress. Subjects were 188 nursing students. data were analyzed using descriptive statistics, Independent t-test, ANOVA, Pearson's correlation coefficient, multiple regression with SPSS/WIN 22.0. The degree of job seeking stress was $2.23{\pm}0.64$. there were negative correlation between job seeking stress and ego-resilience(r=-.353, p<.001), appearance satisfaction(r=-.261, p<.001), career motivation(r=-.289, p<.001). The factors influencing job seeking stress were ego-resilience, appearance satisfaction. These factors explained 21.0% of the variance. These results suggest that development of practice program to reduce job seeking stress and survey about various factors influencing job seeking stress of nursing students'.

Comparative Analysis of Essential Tasks and Delegable Tasks among Kindergarten Dietitians (유치원 유형에 따른 영양(교)사의 필수 업무 및 위임 가능 업무 비교·분석)

  • Kyung, Min Sook;Shin, Yu Lee;Ham, Sunny
    • Journal of the Korean Dietetic Association
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    • v.27 no.4
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    • pp.209-231
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    • 2021
  • The purpose of this study was to compare differences between essential tasks and delegable tasks among public kindergarten dietitians. A survey study was conducted through a self-administered online method from November 18 to December 28, 2019. The survey consisted of essential tasks and delegable tasks, including 6 Duties, 25 Tasks, and 94 Task Elements. The survey was distributed to a sample of 500 kindergartens in Korea, after excluding incomplete surveys and outliers, and a total of 224 responses were used for the analysis. Descriptive statistics were used to compare essential tasks and delegable tasks. The results show that 'Duty A. Nutrition Management', 'Duty B. Foodservice Management Practices', 'Duty C. Hygiene management of kindergarten foodservice', 'Duty D. Nutrition-Diet Education and counseling', and 'Duty F. Professionalism Enhancement' were recognized as essential tasks to be performed by kindergarten dietitians. All 16 tasks elements (100.0%) in 'Duty E. Managing snacks during semesters, and lunch/snack during breaks' were identified as delegable tasks. In conclusion, most tasks were recognized as essential tasks to be performed by kindergarten dietitians. On the other hand, 'Duty E. Managing Snacks during semesters, and lunch/snack during breaks' was considered a delegable task by public-attached kindergarten dietitians. It is recommended that public-attached kindergartens should consider additional workforce related to 'Duty E'. This study is expected to offer basic data on laws and regulations about the duties of kindergarten dietitians.

Green Synthesis of Copper Nano-Drug and Its Dental Application upon Periodontal Disease-Causing Microorganisms

  • El-Rab, Sanaa M.F. Gad;Basha, Sakeenabi;Ashour, Amal A.;Enan, Enas Tawfik;Alyamani, Amal Ahmed;Felemban, Nayef H.
    • Journal of Microbiology and Biotechnology
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    • v.31 no.12
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    • pp.1656-1666
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    • 2021
  • Dental pathogens lead to chronic diseases like periodontitis, which causes loss of teeth. Here, we examined the plausible antibacterial efficacy of copper nanoparticles (CuNPs) synthesized using Cupressus macrocarpa extract (CME) against periodontitis-causing bacteria. The antimicrobial properties of CME-CuNPs were then assessed against oral microbes (M. luteus. B. subtilis, P. aerioginosa) that cause periodontal disease and were identified using morphological/ biochemical analysis, and 16S-rRNA techniques. The CME-CuNPs were characterized, and accordingly, the peak found at 577 nm using UV-Vis spectrometer showed the formation of stable CME-CuNPs. Also, the results revealed the formation of spherical and oblong monodispersed CME-CuNPs with sizes ranged from 11.3 to 22.4 nm. The FTIR analysis suggested that the CME contains reducing agents that consequently had a role in Cu reduction and CME-CuNP formation. Furthermore, the CME-CuNPs exhibited potent antimicrobial efficacy against different isolates which was superior to the reported values in literature. The antibacterial efficacy of CME-CuNPs on oral bacteria was compared to the synergistic solution of clindamycin with CME-CuNPs. The solution exhibited a superior capacity to prevent bacterial growth. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and fractional inhibitory concentration (FIC) of CME-CuNPs with clindamycin recorded against the selected periodontal disease-causing microorganisms were observed between the range of 2.6-3.6 ㎍/ml, 4-5 ㎍/ml and 0.312-0.5, respectively. Finally, the synergistic antimicrobial efficacy exhibited by CME-CuNPs with clindamycin against the tested strains could be useful for the future development of more effective treatments to control dental diseases.

Deep Learning for Classification of High-End Fashion Brand Sensibility (딥러닝을 통한 하이엔드 패션 브랜드 감성 학습)

  • Jang, Seyoon;Kim, Ha Youn;Lee, Yuri;Seol, Jinseok;Kim, Seongjae;Lee, Sang-goo
    • Journal of the Korean Society of Clothing and Textiles
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    • v.46 no.1
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    • pp.165-181
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    • 2022
  • The fashion industry is creating innovative business models using artificial intelligence. To efficiently utilize artificial intelligence (AI), fashion data must be classified. Until now, such data have been classified focusing only on the objective properties of fashion products. Their subjective attributes, such as fashion brand sensibilities, are holistic and heuristic intuitions created by a combination of design elements. This study aims to improve the performance of collaborative filtering in the fashion industry by extracting fashion brand sensibility using computer vision technology. The image data set of fashion brand sensibility consists of high-end fashion brand photos that share sensibilities and communicate well in fashion. About 26,000 fashion photos of 11 high-end fashion brand sensibility labels have been collected from the 16FW to 21SS runway and 50 years of US Vogue magazines beginning from 1971. We use EfficientNet-B1 to establish the main architecture and fine-tune the network with ImageNet-ILSVRC. After training fashion brand sensibilities through deep learning, the proposed model achieved an F-1 score of 74% on accuracy tests. Furthermore, as a result of comparing AI machine and human experts, the proposed model is expected to be expanded to mass fashion brands.

Simultaneous Quantitative Analysis of Natural Complex Extract of Codonopsis lanceolata (Siebold & Zucc.) Trautv., Platycodon grandiflorum A. De Candolle, Glycyrrhiza uralensis Fischer (더덕, 길경, 감초 복합추출물에 대한 지표성분 동시분석 및 함량평가)

  • Min Sung Ko;Chung Hyun Lee;Seul Lee;Ye Seul Kim;Ho Sik Yoon;So-Young Park
    • Korean Journal of Pharmacognosy
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    • v.54 no.1
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    • pp.44-51
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    • 2023
  • Fine dust has emerged as seriously hazardrous particles, which can cause respiratory and cardiovascular diseases. Thus, we have developed a natural complex extract consisted of Codonopsis lanceolata (Siebold & Zucc.) Trautv., Platycodon grandiflorum A. De Candolle, and Glycyrrhiza uralensis Fischer in the ratio of 3 : 5 : 2, which have a beneficial effect on respiratory system. In this study, simultaneous quantitative analysis of marker compounds, lobetyolin, platycodin D, and glycyrrhizic acid in the natural complex extract was developed and validated with high performance liquid chromatography-photodiode array detector. The marker compounds were shown in a large linearity with a correlation coefficient (R2) of 1.000. The limit of detection (LOD) of lobetyolin, platycodin D and glycyrrhizic acid were 5.40 ㎍/mL, 3.94 ㎍/mL and 5.86 ㎍/mL, respectively. The limit of quantification (LOQ) of lobetyolin, platycodin D and glycyrrhizic acid were 16.36 ㎍/mL, 11.94 ㎍/mL and 17.75 ㎍/mL, respectively. The content analysis revealed that the amounts of lobetyolin, platycodin D, and glycyrrhizic acid were 0.039±0.013 mg/g, 0.337±0.048 mg/g, and 1.171±0.003 mg/g, respectively, in the natural complex extract. Therefore, these results could be used as basic data for the standardization and quality control of the natural complex extract.