The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Notes on Five Unrecorded Endophytic Fungi Isolated from Coniferous Leaves and Orchid Roots in Korea

한국에 서식하는 침엽수의 잎과 난초과 식물의 뿌리에서 분리한 5종의 국내 미기록 내생균

  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Lee, Bong-Hyung (Department of Biology Education, Korea National University of Education) ;
  • Bae, Yu-Ra (Department of Biology Education, Korea National University of Education) ;
  • Kim, Dong-Yeo (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 박혁 (한국교원대학교 생물교육과) ;
  • 이봉형 (한국교원대학교 생물교육과) ;
  • 배유라 (한국교원대학교 생물교육과) ;
  • 김동여 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2016.11.26
  • Accepted : 2016.12.13
  • Published : 2016.12.30
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Abstract

We collected leaves of Pinus koraiensis and Thuja koraiensis and roots of Bletilla striata from various sites in Korea. The leaf and root samples were surface-sterilized and endophytic fungi were isolated. Fungal isolates were identified based on their morphological characteristics and a phylogenetic analysis of internal transcribed spacer regions, large subunit regions, and the ${\beta}$-tubulin gene. Consequently, we identified five species of endophytic fungi, namely Colletotrichum simmondsii, Fusarium sterilihyphosum, Diatrypella pulvinata, Ochroconis globalis, and Sphaeria chrysosperma. These species have not been previously reported in Korea and we report them here with descriptions and illustrations.

본 연구에서는 잣나무, 눈측백의 침엽과 자란의 뿌리에서 식물에 공생하는 내생균을 분리하여 동정하였다. 이를 위해 specific primer인 ITS1F와 ITS4를 이용하여 internal transcribed spacer (ITS) rDNA영역과 primer LR0R과 LR 16을 이용하여 large subunit rDNA 영역을, primer Bt2a과 Bt2b를 이용하여 ${\beta}$-tubulin 영역을, 그리고 EF-1 primer를 이용하여 translation elongation factor 지역의 염기서열을 동시에 분석하였다. 연구 결과 Colletotrichum simmondsii, Fusarium sterilihyphosum, Diatrypella pulvinata, Ochroconis globalis, Sphaeria chrysosperma 등 국내 미기록 균주 5종을 분리하여 형태적, 분자생물학적 동정 결과를 서술하였다.

Keywords

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