• Journal of the Korean Society of Food Science and Nutrition
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• v.16 no.4
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• pp.251-261
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• 1987

This study was attempted to increase the production of L-Threonine by Brevibacterium Flavum ATCC 14067, To select the strain which produce the highest threonine, mutants ere induced by N-methyl-N'-nitro-N-nitrosoguanidine treatment. The composition of media and cultural condition for its overproduction of threonine were also studied. In a threonine producer, strain B-13(Met-) was the strain producing the highest amount of threonige among methionine, lysine and isoleucine auxotrophs. The following results were obtained. 1. The wild strain and B-13(Met-) produced threonine 1.4mg/ml and 4.86mg/ml , respectively. 2. The optimum composition of medium for producing threonine by Brevibacterium Flavum B-13 was glucose 10%, ammonium sulfate 4%, potassium phosphate monobasic 0.2%, magnesium sulfate 0.05%, biotin $200{\mu}l$, thiamine $300{\mu}l$. Addition of nicotinic acid also led to increase L-threonine production. 3. In addition of organic nutrients to the fermentation medium, peptone n'ere effective and addition of methionine $100{\mu}g/ml$ produced the highest amount of L-Threonine. Aspartic acid and homoserine were also effective when these amino acid were added to the fermentstion medium. 4. Cultural conditon on threonine production by B-16 were investigated. The optimum pH was 7.0-8.0. The highest amount of threnine was produced after 4 days of cultural period.

• Journal of Microbiology and Biotechnology
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• v.25 no.12
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• pp.1997-2006
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• 2015

Ginsenoside Rg3 is a bioactive ginseng constituent that has been reported to have diverse pathological and physiological effects, including anti-inflammatory and anti-metastatic activities. Metastasis is one of the most important factors involved in patients with melanoma. However, the molecular mechanism underlying the anti-metastatic activities of Rg3 in malignant melanoma cancer has not been fully elucidated. In this study, we have evaluated that Rg3 effectively inhibits metastasis of B16F10 melanoma cancer cells. We found that Rg3 significantly suppresses the migration, invasion, wound healing, and colony-forming abilities of B16F10 cells in a dose-dependent manner. Mechanistically, we demonstrate that Rg3 suppresses B16F10 cell metastasis by inhibiting MMP-13 expression. These results indicate that Rg3 suppresses the metastasis of B16F10 mouse melanoma cancer cells via MMP-13 regulation. Importantly, MMP-13 downregulation may influence the migration and invasion capabilities of melanoma cells and has been correlated with melanoma progression. Therefore, Rg3 is a potential therapeutic candidate that could be used to treat patients with metastatic melanoma.

• Proceedings of the KIEE Conference
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• 1989.07a
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• pp.291-294
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• 1989

The effect of isothermal aging on the crystallization of $Fe_{78}B_{13}Si_9$ metallic glass has been investigated by electrical resistivity, X-ray measurements, bending test, thermal analysis and transmission electron microscpy. Amouphous $Fe_{78}B_{13}Si_9$ alloy was annealed isothermally for 5 to 1200 min. between $300^{\circ}C$ and $540^{\circ}C$. It has been found that close relation between relative resistivity and X-ray diffraction pattern showed. The crystalline peaks of ${\alpha}$-(Fe, Si) and $Fe_2B$ are detected by X-ray experiment. The crystalline phases observed by TEH show ${\alpha}$-(Fe, Si) and $Fe_2B$ with dendritic and cylindrical morphology, respectively. It has been also found that the embrittleness of aged samples rapidly increased with the crystallization and was shown before the crystallization.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.11
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• pp.1235-1239
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• 2010

In this work, a wideband technique has been proposed that improves the IF bandwidth of mixers and a 24-GHz down-conversion mixer employing the proposed technique has been designed and fabricated based on 0.13-${\mu}m$ RFCMOS technology. The mixer showed the conversion gain of $2.7{\pm}1.5$ dB from DC to 5.25 GHz IF for a fixed LO frequency of 24 GHz. Measured P-1dB and LO-RF isolation was -8.7 dBm and 21 dB, respectively. The mixer draws DC current of 10.6 mA from 1.3 V supply.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.7
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• pp.1255-1260
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• 2009

Microstrip interdigital filter is designed with the width and length of a resonator, the gap distance between resonators and the location of a tap. When designing filters, it is a benefit to design with few design parameters comparing to many design parameters. In this paper we design and implement two microstrip interdigital filters operating in the UWB(Ultra Wide-Band) frequency band, one using a fixed width of a resonator and the other using a different width of resonators. The test results of the implemented filters show that the low-band high filter with a fixed width has the insertion loss of 1.49dB, -10dB band width of 720MHz, -35.7dBattenuation at 4.8GHzand below -13dB of S11. The filter with a different width of resonators has the insertion loss of 1.6dB, -10dBbandwidth of 1.63GHz and below-8dBof S11.

• Proceedings of the Korean Magnestics Society Conference
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• 1997.05a
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• pp.80-81
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• 1997

• Proceedings of the Korean Magnestics Society Conference
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• 1993.03a
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• pp.58-59
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• 1993

• Proceedings of the Korean Magnestics Society Conference
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• 1993.03a
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• pp.60-61
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• 1993

• Proceedings of the Korean Magnestics Society Conference
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• 1995.05a
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• pp.56-57
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• 1995

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 1998.11a
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• pp.32-32
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• 1998