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Study Of Millimeter-Wave Passive Imaging Sensor Using the Horn Array Antenna (혼 배열 안테나를 이용한 밀리미터파 수동 이미징 센서 연구)

  • Lim, Hyun-Jun;Chae, Yeon-Sik;Kim, Mi-Ra;Rhee, Jin-Koo
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.47 no.2
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    • pp.74-79
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    • 2010
  • We have designed a millimeter-wave passive imaging sensor with multi-horn antenna array. Six horn array antenna is suggested that it is integrated into one housing, and this antenna is effectively configurated m space to assemble with LNA of WR-10 structure. Antenna is designed to have the peak gain of 17.5dBi at the center frequency of 94GHz, and the return loss of less than -25dB in W-band, and the small aperture size of $6mm{\times}9mm$ for antenna configuration with high resolution. LNA is designed to have total gain of more than 55dB and noise figure of less than 5dB for good sensitivity. We made a detector for DC output translation of millimeter-wave signal with zero bias Schottky diode. It is shown that good sensitivity of more than 500mV/mW.

Biosynthesis of recombinant human prominiinsulin in E. coli and plant systems (대장균과 식물시스템에서 재조합 인간 prominiinsulin 생합성 분석)

  • Choi, Yu Jin;Park, Su Hyun;Kim, Ji Su;Wi, Soo Jin;Park, Ky Young
    • Journal of Plant Biotechnology
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    • v.40 no.3
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    • pp.169-177
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    • 2013
  • Recently, the number of people with diabetes is rapidly increasing, coupled with the fact that the insulin market is remarkably increasing. Therefore, molecular farming for plant-derived pharmaceutical protein production is reported as becoming more attractive than ever. In this study, we carried out experiments step by step for development of recombinant insulin constructs, which were transformed into E. coli system, in vitro transcription and translation system, and tobacco cells. At first, recombinant proinsulin protein was successfully produced in in vitro transcription and translation system with wheat germ extract. After which, recombinant construct of prominiinsulin encoded a fusion protein of 7.8 kDa with trypsin cleavage sites at N terminus and C terminus of minimized C-peptide was tried to in vitro expression using E.coli culture. After purification with His-tag column, the resulting recombinant prominiinsulin protein was processed with trypsin, and then checked insulin biosynthesis by SDS-PAGE and western blot analysis with anti-insulin monoclonal antibody. The immunoreactive product of trypsin-treated miniinsulin was identical to the predicted insulin hexamer. The construct of 35S promoter-driven preprominiinsulin recombinant gene with signal peptide region for ER-targeting and red fluorescence protein gene [N terminus ${\rightarrow}$ tobacco E2 signal peptide ${\rightarrow}$ B-peptide (1-29 AA) ${\rightarrow}$ AAK ${\rightarrow}$ A-peptide (1-21 AA) ${\rightarrow}$ RR ${\rightarrow}$ His6 ${\rightarrow}$ KDEL ${\rightarrow}$ C terminus] was transformed into BY-2 tobacco cells. A polypeptide corresponding to the 38-kDa molecular mass predicted for fusion protein was detected in total protein profiles from transgenic BY-2 cells by western analysis. Therefore, this recombinant preprominiinsulin construct can be used for generation of transgenic tobacco plants producing therapeutic recombinant insulin.

Korean Red Ginseng extract ameliorates melanogenesis in humans and induces antiphotoaging effects in ultraviolet B-irradiated hairless mice

  • Saba, Evelyn;Kim, Seung-Hyung;Lee, Yuan Yee;Park, Chae-Kyu;Oh, Jae-Wook;Kim, Tae-Hwan;Kim, Hyun-Kyoung;Roh, Seong-Soo;Rhee, Man Hee
    • Journal of Ginseng Research
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    • v.44 no.3
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    • pp.496-505
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    • 2020
  • Background: Panax ginseng is a marvelous herbal remedy for all ailments of body. That may be why it is called Panax, which means "cure for all". Melanin is a pigment that gives color to our skin; however, increased melanin production can lead to tumor formation. Human exposure to ultraviolet B radiation has increased extensively owing to the increased sunlight due to global warming. Consequently, a phenomenon called photoaging has been observed for all skin colors and types. As a result of this phenomenon, a set of enzymes called matrix metalloproteinases, which serve as degradation enzymes for extracellular matrix proteins, mainly collagen, is increased, causing depletion of collagen and resulting in early wrinkle formation. Methods: Therefore, in our study, we used the murine melanoma cell line B16/F10 to study the inhibition of melanogenesis by Korean Red Ginseng (KRG) extract in vitro and HRM-2 hairless mice exposed to artificial ultraviolet B to examine the efficacy of KRG in vivo. We prepared a 3% red ginseng extract cream and evaluated its effects on human skin. Results: Our results demonstrated that KRG induced potent suppression of tyrosinase activity and melanin production in B16/F10 cells; moreover, it reduced the transcription and translation of components involved in the melanin production pathway. In the in vivo experiments, KRG potently suppressed the expression of matrix metalloproteinases, reduced wrinkle formation, and inhibited collagen degradation. On human skin, ginseng cream increased skin resilience and skin moisture and enhanced skin tone. Conclusion: Therefore, we conclude that KRG is an excellent skin whitening and antiaging product.

An Analysis of Errors in Describing Solving Process for High School Geometry and Vectors (고등학교 기하와 벡터 과목에서 풀이과정 서술의 오류 분석)

  • Hwang, Jae-woo;Boo, Deok Hoon
    • The Mathematical Education
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    • v.56 no.1
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    • pp.63-80
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    • 2017
  • By analysing the examination papers from third grade high school students, we classified the errors occurred in the problem solving process of high school 'Geometry and Vectors' into several types. There are five main types - (A)Insufficient Content Knowledge, (B)Wrong Method, (C)Logical Invalidity, (D)Unskilled Expression and (E)Interference.. Type A and B lead to an incorrect answer, and type C and D cannot be distinguished by multiple-choice or closed answer questions. Some of these types are classified into subtypes - (B1)Incompletion, (B2)Omitted Condition, (B3)Incorrect Calculation, (C1)Non-reasoning, (C2)Insufficient Reasoning, (C3)Illogical Process, (D1)Arbitrary Symbol, (D2)Using a Character Without Explanation, (D3) Visual Dependence, (D4)Symbol Incorrectly Used, (D5)Ambiguous Expression. Based on the these types of errors, answers of each problem was analysed in detail, and proper ways to correct or prevent these errors were suggested case by case. When problems that were used in the periodical test were given again in descriptive forms, 67% of the students tried to answer, and 14% described flawlessly, despite that the percentage of correct answers were higher than 40% when given in multiple-choice form. 34% of the students who tried to answer have failed to have logical validity. 37% of the students who tried to answer didn't have enough skill to express. In lessons on curves of secondary degree, teachers should be aware of several issues. Students are easily confused between 'focus' and 'vertex', and between 'components of a vector' and 'coordinates of a point'. Students often use an undefined expression when mentioning a parallel translation. When using a character, students have to make sure to define it precisely, to prevent the students from making errors and to make them express in correct ways.

The Terminal and Internal Hairpin Loops of the ctRNA of Plasmid pJB01 Play Critical Roles in Regulating Copy Number

  • Kim, Sam Woong;Jeong, In Sil;Jeong, Eun Ju;Tak, Je Il;Lee, John Hwa;Eo, Seong Kug;Kang, Ho Young;Bahk, Jeong Dong
    • Molecules and Cells
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    • v.26 no.1
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    • pp.26-33
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    • 2008
  • The plasmid pJB01, a member of the pMV158 family isolated from Enterococcus faecium JC1, contains three open reading frames, copA, repB, and repC. Plasmids included in this family produce counter-transcribed RNA (ctRNA) that contributes to copy number control. The pJB01 ctRNA, a transcript which consists of 54 nucleotides (nts), is encoded on the opposite strand from the copA/repB intergenic region and partially overlaps an atypical ribosome binding site (ARBS) for repB. The ARBS is integrated by the two underlined conserved regions: 5'-TTTTTGTNNNNTAANNNNNNNNNATG-3', and the ctRNA is complementary only to the 5' conserved sequence 5'-TTTTTGT-3'. This complementary sequence is located at a distance from the terminal loop of the ctRNA secondary structure. The ctRNA structure predicted by the mfold program suggests the possible generation of a terminal and an internal hairpin loop. The amount of in vitro translation product of repB mRNA was inversely proportional to the ctRNA concentration. Mutations in the terminal and internal hairpin loops of the ctRNA had inhibitory effects on its binding to the target mRNA. We propose that the intact structures of the terminal and internal hairpin loops, respectively, play important roles in forming the initial kissing and extending complexes between the ctRNA and target mRNA and that these regulate the copy number of this plasmid.

The Influence of the Nucleotide Sequences of Random Shine-Dalgarno and Spacer Region on Bovine Growth Hormone Gene Expression

  • Paik Soon-Young;Ra Kyung Soo;Cho Hoon Sik;Koo Kwang Bon;Baik Hyung Suk;Lee Myung Chul;Yun Jong Won;Choi Jang Won
    • Journal of Microbiology
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    • v.44 no.1
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    • pp.64-71
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    • 2006
  • To investigate the effects of the nucleotide sequences in Shine-Dalgarno (SD) and the spacer region (SD-ATG) on bovine growth hormone (bGH) gene expression, the expression vectors under the control of the T7 promoter (pT7-7 vector) were constructed using bGH derivatives (bGH1 & bGH14) which have different 5'-coding regions and were induced in E. coli BL21 (DE3). Oligonucleotides containing random SD sequences and a spacer region were chemically synthesized and the distance between the SD region and the initiation codon were fixed to nine bases in length. The oligonucleotides were annealed and fused to the bGH1 and bGH14 cDNA, respectively. When the bGH gene was induced with IPTG in E. coli BL21(DE3), some clones containing only bGH14 cDNA produced considerable levels of bGH in the range of $6.9\%\;to\;8.5\%$ of total cell proteins by SDS-PAGE and Western blot. Otherwise, the bGH was not detected in any clones with bGH1 cDNA. Accordingly, the nucleotide sequences of SD and the spacer region affect on bGH expression indicates that the sequences sufficiently destabilize the mRNA secondary structure of the bGH14 gene. When the free energy was calculated from the transcription initiation site to the +51 nucleotide of bGH cDNA using a program of nucleic acid folding and hybridization prediction, the constructs with values below -26.3 kcal/mole (toward minus direction) were not expressed. The constructs with the original sequence of bGH cDNA also did not show any expression, regardless of the free energy values. Thus, the disruption of the mRNA secondary structure may be a major factor regulating bGH expression in the translation initiation process. Accordingly, the first stem-loop among two secondary structures present in the 5'-end region of the bGH gene should be disrupted for the effective expression of bGH.

Enhanced Expression of ${\beta}-Xylosidase$ of Bacillus stearothemophilus No. 236 by Change of Translational Initiation Codon in Escherichia coli and Bacillus subtilis

  • Kim, Mi-Dong;Kim, Kyung-Nam;Choi, Yong-Jin
    • Journal of Microbiology and Biotechnology
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    • v.13 no.4
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    • pp.584-590
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    • 2003
  • The xylA gene of Bacillus stearothermophilus No. 236 encoding ${\beta}-xylosidase$, a major xylanolytic enzyme, was previously cloned and sequenced by the present authors. Sequence analysis indicated that translation of the xylA gene was initiated from the noncanonical initiation codon UUG, confirmed by analyzing three different amber (UAG) mutants of the xylA gene. In the present study, the UUG initiation codon was mutated into AUG or GUG, and the effects of the mutations on the XylA synthesis were examined. The AUG initiation codon was found to direct the highest level of ${\beta}-xylosidase$ synthesis; three-fold and fourteen-fold more enzyme activity than the UUG codon in E. coli and B. subtilis cells, respectively. Surprisingly, contrary to other systems reported to date, the UUG start codon was found next to AUG in the relative order of translational efficiency in both organisms. In addition, a greater abundance of the xylA mRNA was detected with the AUG start codon in both of these host cells than with GUG or UUG. Northern blot and Toeprint assays revealed that this was due to enhanced stability of mRNA with the AUG initiation codon. As expected, the ${\beta}-xylosidase$ protein level in the bacterial cells containing mRNA with the AUC start codon was also much higher than the levels with the other two different mRNAs.

Design of an Efficient FTL Algorithm for Flash Memory Accesses Using Sector-level Mapping (섹터 매핑 기법을 적용한 효율적인 FTL 알고리듬 설계)

  • Yoon, Tae-Hyun;Kim, Kwang-Soo;Hwang, Sun-Young
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.12B
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    • pp.1418-1425
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    • 2009
  • This paper proposes a novel FTL (Flash Translation Layer) algorithm based on sector-level mapping to reduce the number of total erase operations in flash memory accesses. The proposed algorithm can reduce the number of erase operations by utilizing the sector-level mapping table when writing data at flash memory. Sector-level mapping technique reduces flash memory access time and extendsthe life time of the flash memory. In the algorithm, wear-leveling is implemented by selecting victim blocks having the minimal number of erase operations, when empty spaces for write are not available. To evaluate the performance of the proposed FTL algorithm, experiments were performed on several applications, such as MP3 players, MPEG players, web browsers and document editors. The proposed algorithm reduces the number of erase operations by 72.4% and 61.9%, when compared with well-known BAST and FAST algorithms, respectively.

A Unicode based Deep Handwritten Character Recognition model for Telugu to English Language Translation

  • BV Subba Rao;J. Nageswara Rao;Bandi Vamsi;Venkata Nagaraju Thatha;Katta Subba Rao
    • International Journal of Computer Science & Network Security
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    • v.24 no.2
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    • pp.101-112
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    • 2024
  • Telugu language is considered as fourth most used language in India especially in the regions of Andhra Pradesh, Telangana, Karnataka etc. In international recognized countries also, Telugu is widely growing spoken language. This language comprises of different dependent and independent vowels, consonants and digits. In this aspect, the enhancement of Telugu Handwritten Character Recognition (HCR) has not been propagated. HCR is a neural network technique of converting a documented image to edited text one which can be used for many other applications. This reduces time and effort without starting over from the beginning every time. In this work, a Unicode based Handwritten Character Recognition(U-HCR) is developed for translating the handwritten Telugu characters into English language. With the use of Centre of Gravity (CG) in our model we can easily divide a compound character into individual character with the help of Unicode values. For training this model, we have used both online and offline Telugu character datasets. To extract the features in the scanned image we used convolutional neural network along with Machine Learning classifiers like Random Forest and Support Vector Machine. Stochastic Gradient Descent (SGD), Root Mean Square Propagation (RMS-P) and Adaptative Moment Estimation (ADAM)optimizers are used in this work to enhance the performance of U-HCR and to reduce the loss function value. This loss value reduction can be possible with optimizers by using CNN. In both online and offline datasets, proposed model showed promising results by maintaining the accuracies with 90.28% for SGD, 96.97% for RMS-P and 93.57% for ADAM respectively.

Epitope Tagging with a Peptide Derived from the preS2 Region of Hepatitis B Virus Surface Antigen

  • Kang, Hyun-Ah;Yi, Gwan-Su;Yu, Myeong-Hee
    • BMB Reports
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    • v.28 no.4
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    • pp.353-358
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    • 1995
  • Epitope tagging is the process of fusing a set of amino acid residues that are recognized as an antigenic determinant to a protein of interest. Tagging a protein with an epitope facilitates various immunochemical analyses of the tagged protein with a specific monoclonal antibody. The monoclonal antibody H8 has subtype specificity for an epitope derived from the preS2 region of hepatitis B virus surface antigen. Previous studies on serial deletions of the preS2 region indicated that the preS2 epitope was located in amino acid residues 130~142. To test whether the amino acid sequence in this interval is sufficient to confer on proteins the antigenicity recognizable by the antibody H8, the set of amino acid residues in the interval was tagged to the amino terminal of ${\beta}$-galactosidase and to the carboxyl terminal of the truncated $p56^{lck}$ fragment. The tagged ${\beta}$-galactosidase, expressed in Escherichia coli, maintained the enzymatic activity and was immunoprecipitated efficiently with H8. The tagged $p56^{lck}$ fragment, synthesized in an in vitro translation system, was also immunoprecipitated specifically with H8. These results demonstrate that the amino acid sequence of the preS2 region can be used efficiently for the epitope tagging approach.

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