• Title/Summary/Keyword: B-translation

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PelB Signal Sequence로 유도된 재조합 인간 상피세포 증식인자 분비 발현 벡터의 제조

  • Park, Se-Cheol;Nam, Jung-Hyun;Kim, Jeong-Keun;Kwon, Tae-Jong;Ko, In-Young;You, Kwang-Hyun
    • Microbiology and Biotechnology Letters
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    • v.24 no.5
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    • pp.553-559
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    • 1996
  • We have designed nucleotide sequences of hEGF structural gene to eliminate the N-terminal methionine residue incorporated during the translation initiation step, and constructed recombinant human epidermal growth factor (rhEGF) secretion plasmids pYHB101, and pYHB2 in which pelB signal sequence-hEGF gene was expressed under the control of the T7, and tac promoter, respectively. We also constructed pYHB1 vector which contains rhEGF gene controlled by T7 promoter. The transformant with pYHB101 showed relatively slow growth pattern compared to the transformant with pYHB1. However, we observed that the transformant with pYHB101 secreted rhEGF of 13 mg/l significantly after 5 hr induction with 1 mM IPTG and that the T7 promoter was more effective than tac promoter when connected to pelB signal sequence. The amount of rhEGF was 14 mg/l under the sub-optimized condition.

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First Report of an Apple Ring Rot Fungus Botryosphaeria kuwatsukai in Korea

  • Gwang-Jae Lim;Kallol Das;Hyeong-Jin Noh;Seong-Keun Lim;Young-Je Cho;Seung-Yeol Lee;Hee-Young Jung;Seong Hwan Kim
    • The Korean Journal of Mycology
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    • v.51 no.2
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    • pp.111-120
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    • 2023
  • Three fungal strains designated as KNUF-23-MG32, KNUF-23-YC8, and KNUF-23-MJ82 were isolated from the abnormal symptomatic apple trees during screening fungal pathogens collected in Jeollanam-do and Gyeongsangbuk-do, Korea. These fungal strains were found to have similar cultural and morphological characteristics close to the genus Botryosphaeria. Morphological characteristics were matched with B. kuwatsukai CBS 135219 but different with B. dothidea KACC 45481 and B. sinensis HMAS 246714T. Pathogenicity tests of strain KNUF-23-MG32 showed that this strain causes rot in Fuji apples. Molecular phylogenetic analysis based on the sequences of the internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (TEF1α), and partial β-tubulin (TUB2) showed that these strains are B. kuwatsukai. To the best of our knowledge, this is the first report of an apple ring rot fungus Botryosphaeria kuwatsukai in Korea.

Immunoadjuvant Activity of Korean Mistletoe Lectin B-chain (한국산 겨우살이 Lectin B-chain의 면역증강 효과)

  • Her, Sun-Mi;An, Hyo-Sun;Kim, Kyu-Dae;Kim, Young-Hoon;Kim, In-Bo;Yoon, Taek-Joon;Kim, Jong-Bae
    • Korean Journal of Pharmacognosy
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    • v.42 no.3
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    • pp.246-252
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    • 2011
  • Korean mistletoe Lectin (KML-C) is composed of A and B sub-chain. B chain binds to carbohydrates on cell surface and A chain hinders translation and induces an apoptosis as a RIP (ribosome inactivating protein). KML-C has very strong biological activities, it has seriously limits to use as a cancer therapy or adjuvant because of its toxicity to normal cells. This study is therefore conducted to see if B chain of KML-C might have immunological activity, especially adjuvant activities with less toxicity. We isolated B chain from KML-C using the lactose affinity chromatography, and examined their immunoadjuvant activity. The isolated B-chain did not show any cytotoxicity against tumor cell, RAW264.7, and P388D1 while KML-C had a very strong toxicity. This non-toxic effect was observed also by in-vivo study. Both humoral and cellular immunities were observed ; the antibody titer was increased when the mice were immunized with B-chain used as adjuvant like Freund's adjuvant, indicating that B chain of mistletoe lectin alone might be used for adjuvant; it also increased DTH in cellular immunity. These results suggest that B-chain of KML-C might be used for adjuvant used for the production of antibody or vaccine with less toxicity.

ZigBee Service Gateway Enabling Dynamic Reconfiguration (동적 설정 가능한 지그비 서비스 게이트웨이 구조)

  • Kim, Seong-Hoon;Kang, Jeong-Seok;Baeg, Sung-Ho;Park, Jae-Han;Park, Hong-Seong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.10B
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    • pp.644-654
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    • 2007
  • ZigBee is one of wireless communication standards and many applications based on Zigbee have been developing for commercial applications. Most of those applications require links to Internet like Zigbee service gateways. However, ZigBee service gateway should provides following functions to be effectively utilized itself: translation of messages between diverse ZigBee application profiles and message formats used in Internet, addition/removal of a new ZigBee service without stopping existing services according to addition/removal of a Zigbee node, and no transmission of unwanted packets to ZigBee network. Therefore, this paper proposes a new type of ZigBee Service Gateway, called ZiGate to provide the functions. The proposed ZiGate is capable of re-configurating message translation modules required in case of addition/removal of nodes and doesn't transmit unnecessary packets to the ZigBee networks by synchronizing itself with ZigBee network. And ZiGate provides translation modules between messages defined in Zigbee application profiles and both text-based and binary-based messages used in Internet. In this paper we implements the ZiGate and evaluates it on the test bed. This evaluations show that the proposed ZiGate works efficiently.

An Efficient Index Buffer Management Scheme for a B+ tree on Flash Memory (플래시 메모리상에 B+트리를 위한 효율적인 색인 버퍼 관리 정책)

  • Lee, Hyun-Seob;Joo, Young-Do;Lee, Dong-Ho
    • The KIPS Transactions:PartD
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    • v.14D no.7
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    • pp.719-726
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    • 2007
  • Recently, NAND flash memory has been used for a storage device in various mobile computing devices such as MP3 players, mobile phones and laptops because of its shock-resistant, low-power consumption, and none-volatile properties. However, due to the very distinct characteristics of flash memory, disk based systems and applications may result in severe performance degradation when directly adopting them on flash memory storage systems. Especially, when a B-tree is constructed, intensive overwrite operations may be caused by record inserting, deleting, and its reorganizing, This could result in severe performance degradation on NAND flash memory. In this paper, we propose an efficient buffer management scheme, called IBSF, which eliminates redundant index units in the index buffer and then delays the time that the index buffer is filled up. Consequently, IBSF significantly reduces the number of write operations to a flash memory when constructing a B-tree. We also show that IBSF yields a better performance on a flash memory by comparing it to the related technique called BFTL through various experiments.

Expressed sequence tags analysis of Blattella germanica

  • CHUNG Hyang Suk;YU Tai Hyun;KIM Bong Jin;KIM Sun Mi;KIM Joo Yeong;YU Hak Sun;Jeong Hae Jin;OCK Mee Sun
    • Parasites, Hosts and Diseases
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    • v.43 no.4 s.136
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    • pp.149-156
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    • 2005
  • Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About $42\%$ (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling path-ways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these rep-resent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.

Object-Based Image Retrieval Using Color Adjacency and Clustering Method (컬러 인접성과 클러스터링 기법을 이용한 객체 기반 영상 검색)

  • Lee Hyung-Jin;Park Ki-Tae;Moon Young-Shik
    • The KIPS Transactions:PartB
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    • v.12B no.1 s.97
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    • pp.31-38
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    • 2005
  • This paper proposes an object-based image retrieval scheme using color adjacency and clustering method. Color adjacency features in boundary regions are utilized to extract candidate blocks of interest from image database and a clustering method is used to extract the regions of interest(ROI) from candidate blocks of interest. To measure the similarity between the query and database images, the histogram intersection technique is used. The color pair information used in the proposed method is robust against translation, rotation, and scaling. Consequently, experimental results have shown that the proposed scheme is superior to existing methods in terms of ANMRR.

Efficient Digitizing in Reverse Engineering By Sensor Fusion (역공학에서 센서융합에 의한 효율적인 데이터 획득)

  • Park, Young-Kun;Ko, Tae-Jo;Kim, Hrr-Sool
    • Journal of the Korean Society for Precision Engineering
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    • v.18 no.9
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    • pp.61-70
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    • 2001
  • This paper introduces a new digitization method with sensor fusion for shape measurement in reverse engineering. Digitization can be classified into contact and non-contact type according to the measurement devices. Important thing in digitization is speed and accuracy. The former is excellent in speed and the latter is good for accuracy. Sensor fusion in digitization intends to incorporate the merits of both types so that the system can be automatized. Firstly, non-contact sensor with vision system acquires coarse 3D point data rapidly. This process is needed to identify and loco]ice the object located at unknown position on the table. Secondly, accurate 3D point data can be automatically obtained using scanning probe based on the previously measured coarse 3D point data. In the research, a great number of measuring points of equi-distance were instructed along the line acquired by the vision system. Finally, the digitized 3D point data are approximated to the rational B-spline surface equation, and the free-formed surface information can be transferred to a commercial CAD/CAM system via IGES translation in order to machine the modeled geometric shape.

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Emerging role of anti-proliferative protein BTG1 and BTG2

  • Kim, Sang Hyeon;Jung, In Ryeong;Hwang, Soo Seok
    • BMB Reports
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    • v.55 no.8
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    • pp.380-388
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    • 2022
  • The B cell translocation gene 1 (BTG1) and BTG2 play a key role in a wide range of cellular activities including proliferation, apoptosis, and cell growth via modulating a variety of central biological steps such as transcription, post-transcriptional, and translation. BTG1 and BTG2 have been identified by genomic profiling of B-cell leukemia and diverse lymphoma types where both genes are commonly mutated, implying that they serve as tumor suppressors. Furthermore, a low expression level of BTG1 or BTG2 in solid tumors is frequently associated with malignant progression and poor treatment outcomes. As physiological aspects, BTG1 and BTG2 have been discovered to play a critical function in regulating quiescence in hematopoietic lineage such as Hematopoietic stem cells (HSCs) and naive and memory T cells, highlighting their novel role in maintaining the quiescent state. Taken together, emerging evidence from the recent studies suggests that BTG1 and BTG2 play a central anti-proliferative role in various tissues and cells, indicating their potential as targets for innovative therapeutics.

Gene Expression Profiles of HeLa Cells Impacted by Hepatitis C Virus Non-structural Protein NS4B

  • Zheng, Yi;Ye, Lin-Bai;Liu, Jing;Jing, Wei;Timani, Khalid A.;Yang, Xiao-Jun;Yang, Fan;Wang, Wei;Gao, Bo;Wu, Zhen-Hui
    • BMB Reports
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    • v.38 no.2
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    • pp.151-160
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    • 2005
  • By a cDNA array representing 2308 signal transduction related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RT-PCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeletion and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.