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Expressed sequence tags analysis of Blattella germanica  

CHUNG Hyang Suk (Department of Otorhinolaryngology, Kosin University College of Medicine)
YU Tai Hyun (Department of Otorhinolaryngology, Kosin University College of Medicine)
KIM Bong Jin (Department of Parasitology, Kosin University College of Medicine)
KIM Sun Mi (Department of Parasitology, Kosin University College of Medicine)
KIM Joo Yeong (Department of Parasitology, Kosin University College of Medicine)
YU Hak Sun (Department of Parasitology, Pusan National University College of Medicine)
Jeong Hae Jin (Department of Parasitology, Pusan National University College of Medicine)
OCK Mee Sun (Department of Parasitology, Kosin University College of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.43, no.4, 2005 , pp. 149-156 More about this Journal
Abstract
Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About $42\%$ (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling path-ways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these rep-resent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.
Keywords
Blattella germanica; cDNA library; ESTs; BLAST search; novel genes;
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