Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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PelB Signal Sequence로 유도된 재조합 인간 상피세포 증식인자 분비 발현 벡터의 제조

  • Park, Se-Cheol ;
  • Nam, Jung-Hyun ;
  • Kim, Jeong-Keun ;
  • Kwon, Tae-Jong ;
  • Ko, In-Young ;
  • You, Kwang-Hyun
  • 박세철 ((주)유한양행 중앙연구소 생물제품연구실) ;
  • 남정현 ((주)유한양행 중앙연구소 생물제품연구실) ;
  • 김정근 ;
  • 권태종 ((주)유한양행 중앙연구소 생물제품연구실) ;
  • 고인영 ;
  • 유광현 (건국대학교 미생물학과)
  • Published : 1996.10.01
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Abstract

We have designed nucleotide sequences of hEGF structural gene to eliminate the N-terminal methionine residue incorporated during the translation initiation step, and constructed recombinant human epidermal growth factor (rhEGF) secretion plasmids pYHB101, and pYHB2 in which pelB signal sequence-hEGF gene was expressed under the control of the T7, and tac promoter, respectively. We also constructed pYHB1 vector which contains rhEGF gene controlled by T7 promoter. The transformant with pYHB101 showed relatively slow growth pattern compared to the transformant with pYHB1. However, we observed that the transformant with pYHB101 secreted rhEGF of 13 mg/l significantly after 5 hr induction with 1 mM IPTG and that the T7 promoter was more effective than tac promoter when connected to pelB signal sequence. The amount of rhEGF was 14 mg/l under the sub-optimized condition.

Keywords

References

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