• Biomedical Science Letters
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• v.9 no.2
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• pp.105-110
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• 2003

Ankyrins are a ubiquitously expressed family of intracellular adaptor proteins involved in targeting diverse proteins to specialized membrane domains in both the plasma membrane and the endoplasmic reticulum. Canonical ankyrins are 190-220 kDa proteins expressed in most tissues and cell types and comprise a membrane-binding domain (MBD) of 24 ANK repeats, a spectrin-binding domain, a death domain and a C-terminal domain. Rescue studies with ankyrin-B/G chimeras have identified the C-terminal domain of ankyrin-B as the defining domain in specifying ankyrin-B activity, but the function of C-terminal domain of ankyrin-B is, however, not known. We report here that the C-terminal domain of ankyrin-B is capable of interacting with the C-terminal Region of Hsp40. The Hsps are induced not only by heat shock but also by various other environmental stresses. Hsps are also expressed constitutively at normal growth temperatures and have basic and indispensable functions in the life cycle of proteins as molecular chaperones, as well as playing a role in protecting cells from the deleterious stresses. The binding sites required in the interaction between C-terminal domain of ankyrin-B and C-terminal region of Hsp40 were characterized using the yeast two-hybrid system and GST-pull down assay. The interaction between ankyrin-B and Hsp40 represents the first direct evidence of ankyrin's role as chaperones.

• The Transactions of the Korea Information Processing Society
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• v.4 no.10
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• pp.2512-2520
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• 1997

ITU-T Q.2931 and ATM Forum UNI 3.1 lead the standardization of signaling functions for the call/connection control at B-ISDN UNI. The one is applicable to the system in the public network, which provides the point-to-point call/connection control. The other is applicable to configure ATM private network mainly related with various terminal equipments. Domestic B-ISDN developers have developed ATM switches and B-NTs as network equipment, and B-TA as a terminal equipment. But many kinds of terminal-equipments are needed to provide various services in B-ISDN. If the public network can accommodate the terminal-equipments of UNI 3.1, it's very effective to accommodate manu kinds of terminal-equipments. Therefore, this paper identifies the problems that can be occurred on the signaling procedure, when B-ISDN the public network is connected to the UNI 3.1 terminal-equipment, and provides good alternatives that can handle this kind of call/connection well.

• The Pure and Applied Mathematics
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• v.5 no.1
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• pp.45-54
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• 1998

We study the space-times that have a unique terminal indecomposable past set or a unique terminal indecomposable future set and examine their causal boundary, and we investigate some conditions for the warped product space-times of the form (a, b) ${\times}_fF$ to have a unique terminal indecomposable past set or a unique terminal indecomposable future set.

• Journal of Microbiology and Biotechnology
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• v.6 no.5
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• pp.361-363
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• 1996

The terminal regions of the double-stranded RNA (dsRNA) genome segments of infectious pancreatic necrosis virus (IPNV) DRT strain were sequenced. The dsRNAs, which were $^{32}P$-labelled at their 3'-termini by incubation with [$^{32}P$]pCp and T4 RNA ligase, were separated by 5$%$ polyacrylamide gel electrophoresis, and the segments A and B of IPNV-DRT were sequenced by two-dimensional gel electrophoresis. The 5'-terminal sequences of the IPNV-DRT plus strand from two genome segments were found to have the same conserved nucleotide (5'-CGG(C/A)A-), but the 3'-terminal sequences -CCCCAGGCG-3' and -CGGACCCCG-3' were found in the plus strand from segments A and B, respectively. The inverted oligonucleotide sequences of 3'-terminal of between segments A and B were found and they differ from those of other IPNVs.

• Journal of the Korean Institute of Rural Architecture
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• v.17 no.3
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• pp.27-36
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• 2015

This research studies the adequate size standard of Seogwipo cruise terminal CIQ facility that is scheduled to be built around Gangjeong harbor area in Seogwipo-city. In order to respond to the highly increasing number of passenger cruise ships compared to Seogwipo cruise terminal design in 2010, the adequate size standard of Seogwipo cruise terminal CIQ facility was examined for passenger service level grade. Based on size computation elements such as the number of passengers of cruise ships with the largest size of port entry, ship landing rate, passenger processing ratio, and surge factor, the CIQ facility size for each service level grade was reviewed. As a result, the area of 2,971m2 (A grade), 2,409 m2 (B grade), and 2,088 m2 (C grade) were computed. This showed that the area of B grade was about 82% and C grade 70% compared to the area of A grade. The CIQ facility size computed for each service level grade in this research was analyzed that its area needed to be increased by 322% at least and 458% at most, compared to the CIQ facility area of 649m2 of the existing design (2010). In order to respond to the increasing number of cruise passengers, provide high-level passenger service, and improve the international image of Jeju, Seogwipo cruise terminal should secure the size that is equal to or higher than the B grade of service level.

• Bulletin of the Korean Mathematical Society
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• v.54 no.5
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• pp.1471-1483
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• 2017

We classify three-dimensional non-normal semi-terminal singularities.

• Journal of Hospice and Palliative Care
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• v.19 no.2
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• pp.154-162
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• 2016

Purpose: This descriptive study was aimed at identifying the relations among geriatric nurses' terminal care performance, death anxiety and self-esteem and the factors that affect nurses' terminal care stress. Methods: Data were collected using a self-reported questionnaire completed by 212 geriatric hospital nurses working in 10 hospitals in K city and B metropolitan city. Results: The survey results showed that the stress factors were terminal care performance and death anxiety. Significant predictors for terminal care stress were death anxiety and terminal care performance. (And the higher the level of death anxiety and terminal care performance were, the heavier the stress was.) These factors explained 32.5% of the variance in terminal care stress. Conclusion: The results of the study suggested that terminal care performance was an important factor of terminal care stress for geriatric nurses. Therefore, it seems that it is necessary to develop an educational intervention program to improve nurses' terminal care performance to reduce their terminal care stress.

• Molecules and Cells
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• v.26 no.1
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• pp.26-33
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• 2008

The plasmid pJB01, a member of the pMV158 family isolated from Enterococcus faecium JC1, contains three open reading frames, copA, repB, and repC. Plasmids included in this family produce counter-transcribed RNA (ctRNA) that contributes to copy number control. The pJB01 ctRNA, a transcript which consists of 54 nucleotides (nts), is encoded on the opposite strand from the copA/repB intergenic region and partially overlaps an atypical ribosome binding site (ARBS) for repB. The ARBS is integrated by the two underlined conserved regions: 5'-TTTTTGTNNNNTAANNNNNNNNNATG-3', and the ctRNA is complementary only to the 5' conserved sequence 5'-TTTTTGT-3'. This complementary sequence is located at a distance from the terminal loop of the ctRNA secondary structure. The ctRNA structure predicted by the mfold program suggests the possible generation of a terminal and an internal hairpin loop. The amount of in vitro translation product of repB mRNA was inversely proportional to the ctRNA concentration. Mutations in the terminal and internal hairpin loops of the ctRNA had inhibitory effects on its binding to the target mRNA. We propose that the intact structures of the terminal and internal hairpin loops, respectively, play important roles in forming the initial kissing and extending complexes between the ctRNA and target mRNA and that these regulate the copy number of this plasmid.

• BMB Reports
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• v.52 no.5
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• pp.330-335
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• 2019

Hepatitis B virus (HBV) encoding the HBV x protein (HBx) is a known causative agent of hepatocellular carcinoma (HCC). Its pathogenic activities in HCC include interference with several signaling pathways associated with cell proliferation and apoptosis. Mutant C-terminal-truncated HBx isoforms are frequently found in human HCC and have been shown to enhance proliferation and invasiveness leading to HCC malignancy. We investigated the molecular mechanism of the reduced doxorubicin cytotoxicity by C-terminal truncated HBx. Cells transfected with C-terminal truncated HBx exhibited reduced cytotoxicity to doxorubicin compared to those transfected with full-length HBx. The doxorubicin resistance of cells expressing C-terminal truncated HBx correlated with upregulation of the ATP binding cassette subfamily B member 1(ABCB1) transporter, resulting in the enhanced efflux of doxorubicin. Inhibiting the activity of ABCB1 and silencing ABCB1 expression by small interfering ribonucleic acid (siRNA) increased the cytotoxicity of doxorubicin. These results indicate that elevated ABCB1 expression induced by C-terminal truncation of HBx was responsible for doxorubicin resistance in HCC. Hence, co-treatment with an ABCB1 inhibitor and an anticancer agent may be effective for the treatment of patients with liver cancer containing the C-terminal truncated HBx.

• BMB Reports
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• v.46 no.3
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• pp.169-174
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• 2013

The human $B_{12}$ trafficking chaperone hCblC is well conserved in mammals and non-mammalian eukaryotes. However, the C-terminal ~40 amino acids of hCblC vary significantly and are predicted to be deleted by alternative splicing of the encoding gene. In this study, we examined the thermostability of the bovine CblC truncated at the C-terminal variable region (t-bCblC) and its regulation by glutathione. t-bCblC is highly thermolabile ($T_m={\sim}42^{\circ}C$) similar to the full-length protein (f-bCblC). However, t-bCblC is stabilized to a greater extent than f-bCblC by binding of reduced glutathione (GSH) with increased sensitivity to GSH. In addition, binding of oxidized glutathione (GSSG) destabilizes t-bCblC to a greater extent and with increased sensitivity as compared to f-bCblC. These results indicate that t-bCblC is a more sensitive form to be regulated by glutathione than the full-length form of the protein.