• 대한의생명과학회지
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• 제9권2호
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• pp.105-110
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• 2003

Ankyrins are a ubiquitously expressed family of intracellular adaptor proteins involved in targeting diverse proteins to specialized membrane domains in both the plasma membrane and the endoplasmic reticulum. Canonical ankyrins are 190-220 kDa proteins expressed in most tissues and cell types and comprise a membrane-binding domain (MBD) of 24 ANK repeats, a spectrin-binding domain, a death domain and a C-terminal domain. Rescue studies with ankyrin-B/G chimeras have identified the C-terminal domain of ankyrin-B as the defining domain in specifying ankyrin-B activity, but the function of C-terminal domain of ankyrin-B is, however, not known. We report here that the C-terminal domain of ankyrin-B is capable of interacting with the C-terminal Region of Hsp40. The Hsps are induced not only by heat shock but also by various other environmental stresses. Hsps are also expressed constitutively at normal growth temperatures and have basic and indispensable functions in the life cycle of proteins as molecular chaperones, as well as playing a role in protecting cells from the deleterious stresses. The binding sites required in the interaction between C-terminal domain of ankyrin-B and C-terminal region of Hsp40 were characterized using the yeast two-hybrid system and GST-pull down assay. The interaction between ankyrin-B and Hsp40 represents the first direct evidence of ankyrin's role as chaperones.

• 한국정보처리학회논문지
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• 제4권10호
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• pp.2512-2520
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• 1997

B-ISDN 사용자 망 접면에서 호/연결 제어를 위한 신호 기능은 ITU-T의 Q.2931과 ATM Forum의 UNI 3.1이 대표적이다. 점대점 호/연결 제어 절차를 제공하는 Q.2931은 공중망을 구성하는 장치에 적용이 되며, ATM Forum의 UNI 3.1은 여러 가지의 단말 장치 위주의 ATM 사설망을 구성하는데 적용된다. 국내의 B-ISDN 개발 사업에서는 방 장치인 ATM소형 교환기, B-NT 등과 단말장치인 B-TA등을 개발하였다. 그러나 B-ISDN에서 다양한 서비스를 제공하기 위해서는 여러 종류의 단말장치들을 필요로 한다. 공중망에서 UNI 3.1 단말 장치를 수용할 수 있다면, 여러 종류의 단말장치들을 확보할 수 있으므로 매우 효율적이다. 그러므로, 본 논문에서는 B-ISDN 공중망에 UNI 3.1 단말 장치를 접속하여 점대점 신호 절차 수행 시에 발생할 수 있는 문제점들을 예측하고, 호/연결이 원만하게 진행될 수 있는 방안들을 제안한다.

• 한국수학교육학회지시리즈B:순수및응용수학
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• 제5권1호
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• pp.45-54
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• 1998

We study the space-times that have a unique terminal indecomposable past set or a unique terminal indecomposable future set and examine their causal boundary, and we investigate some conditions for the warped product space-times of the form (a, b) ${\times}_fF$ to have a unique terminal indecomposable past set or a unique terminal indecomposable future set.

• Journal of Microbiology and Biotechnology
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• 제6권5호
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• pp.361-363
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• 1996

The terminal regions of the double-stranded RNA (dsRNA) genome segments of infectious pancreatic necrosis virus (IPNV) DRT strain were sequenced. The dsRNAs, which were $^{32}P$-labelled at their 3'-termini by incubation with [$^{32}P$]pCp and T4 RNA ligase, were separated by 5$%$ polyacrylamide gel electrophoresis, and the segments A and B of IPNV-DRT were sequenced by two-dimensional gel electrophoresis. The 5'-terminal sequences of the IPNV-DRT plus strand from two genome segments were found to have the same conserved nucleotide (5'-CGG(C/A)A-), but the 3'-terminal sequences -CCCCAGGCG-3' and -CGGACCCCG-3' were found in the plus strand from segments A and B, respectively. The inverted oligonucleotide sequences of 3'-terminal of between segments A and B were found and they differ from those of other IPNVs.

• 한국농촌건축학회논문집
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• 제17권3호
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• pp.27-36
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• 2015

This research studies the adequate size standard of Seogwipo cruise terminal CIQ facility that is scheduled to be built around Gangjeong harbor area in Seogwipo-city. In order to respond to the highly increasing number of passenger cruise ships compared to Seogwipo cruise terminal design in 2010, the adequate size standard of Seogwipo cruise terminal CIQ facility was examined for passenger service level grade. Based on size computation elements such as the number of passengers of cruise ships with the largest size of port entry, ship landing rate, passenger processing ratio, and surge factor, the CIQ facility size for each service level grade was reviewed. As a result, the area of 2,971m2 (A grade), 2,409 m2 (B grade), and 2,088 m2 (C grade) were computed. This showed that the area of B grade was about 82% and C grade 70% compared to the area of A grade. The CIQ facility size computed for each service level grade in this research was analyzed that its area needed to be increased by 322% at least and 458% at most, compared to the CIQ facility area of 649m2 of the existing design (2010). In order to respond to the increasing number of cruise passengers, provide high-level passenger service, and improve the international image of Jeju, Seogwipo cruise terminal should secure the size that is equal to or higher than the B grade of service level.

• 대한수학회보
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• 제54권5호
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• pp.1471-1483
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• 2017

We classify three-dimensional non-normal semi-terminal singularities.

• Journal of Hospice and Palliative Care
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• 제19권2호
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• pp.154-162
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• 2016

목적: 본 연구는 요양병원 간호사의 임종간호수행과 죽음불안, 자아존중감 및 임종간호스트레스간의 상관관계를 확인하고 임종간호스트레스에 영향을 미치는 요인을 파악하기 위한 서술적 조사연구이다. 방법: K시와 B광역시 소재 10개 요양병원에 근무하는 212명의 간호사를 대상으로 자가 보고식 설문지를 이용하여 자료를 수집하였다. 결과: 요양병원간호사의 임종간호스트레스에 영향을 미치는 요인은 죽음불안과 임종간호수행이었으며 죽음불안과 임종간호수행 정도가 높을수록 임종간호스트레스가 높았다. 이들 변수들은 임종간호스트레스에 대하여 32.5%의 설명력을 나타냈다. 결론: 요양병원간호사의 임종간호스트레스를 완화시키기 위하여 효율적인 인력배치 등의 행정적인 방안을 수립하고 죽음불안을 감소시킬 수 있는 임종간호교육프로그램 개발 및 효과검증 연구 수행을 제안하는 바이다.

• Molecules and Cells
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• 제26권1호
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• pp.26-33
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• 2008

The plasmid pJB01, a member of the pMV158 family isolated from Enterococcus faecium JC1, contains three open reading frames, copA, repB, and repC. Plasmids included in this family produce counter-transcribed RNA (ctRNA) that contributes to copy number control. The pJB01 ctRNA, a transcript which consists of 54 nucleotides (nts), is encoded on the opposite strand from the copA/repB intergenic region and partially overlaps an atypical ribosome binding site (ARBS) for repB. The ARBS is integrated by the two underlined conserved regions: 5'-TTTTTGTNNNNTAANNNNNNNNNATG-3', and the ctRNA is complementary only to the 5' conserved sequence 5'-TTTTTGT-3'. This complementary sequence is located at a distance from the terminal loop of the ctRNA secondary structure. The ctRNA structure predicted by the mfold program suggests the possible generation of a terminal and an internal hairpin loop. The amount of in vitro translation product of repB mRNA was inversely proportional to the ctRNA concentration. Mutations in the terminal and internal hairpin loops of the ctRNA had inhibitory effects on its binding to the target mRNA. We propose that the intact structures of the terminal and internal hairpin loops, respectively, play important roles in forming the initial kissing and extending complexes between the ctRNA and target mRNA and that these regulate the copy number of this plasmid.

• BMB Reports
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• 제52권5호
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• pp.330-335
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• 2019

Hepatitis B virus (HBV) encoding the HBV x protein (HBx) is a known causative agent of hepatocellular carcinoma (HCC). Its pathogenic activities in HCC include interference with several signaling pathways associated with cell proliferation and apoptosis. Mutant C-terminal-truncated HBx isoforms are frequently found in human HCC and have been shown to enhance proliferation and invasiveness leading to HCC malignancy. We investigated the molecular mechanism of the reduced doxorubicin cytotoxicity by C-terminal truncated HBx. Cells transfected with C-terminal truncated HBx exhibited reduced cytotoxicity to doxorubicin compared to those transfected with full-length HBx. The doxorubicin resistance of cells expressing C-terminal truncated HBx correlated with upregulation of the ATP binding cassette subfamily B member 1(ABCB1) transporter, resulting in the enhanced efflux of doxorubicin. Inhibiting the activity of ABCB1 and silencing ABCB1 expression by small interfering ribonucleic acid (siRNA) increased the cytotoxicity of doxorubicin. These results indicate that elevated ABCB1 expression induced by C-terminal truncation of HBx was responsible for doxorubicin resistance in HCC. Hence, co-treatment with an ABCB1 inhibitor and an anticancer agent may be effective for the treatment of patients with liver cancer containing the C-terminal truncated HBx.

• BMB Reports
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• 제46권3호
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• pp.169-174
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• 2013

The human $B_{12}$ trafficking chaperone hCblC is well conserved in mammals and non-mammalian eukaryotes. However, the C-terminal ~40 amino acids of hCblC vary significantly and are predicted to be deleted by alternative splicing of the encoding gene. In this study, we examined the thermostability of the bovine CblC truncated at the C-terminal variable region (t-bCblC) and its regulation by glutathione. t-bCblC is highly thermolabile ($T_m={\sim}42^{\circ}C$) similar to the full-length protein (f-bCblC). However, t-bCblC is stabilized to a greater extent than f-bCblC by binding of reduced glutathione (GSH) with increased sensitivity to GSH. In addition, binding of oxidized glutathione (GSSG) destabilizes t-bCblC to a greater extent and with increased sensitivity as compared to f-bCblC. These results indicate that t-bCblC is a more sensitive form to be regulated by glutathione than the full-length form of the protein.