• Journal of Ginseng Research
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• 제42권4호
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• pp.436-446
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• 2018

Background: The potential therapeutic values of Korean Red Ginseng extract (KRGE) in autoimmune disorders of nervous system have not been fully investigated. Methods: We used an acute experimental autoimmune encephalomyelitis animal model of multiple sclerosis and determined the effects and mechanism of KRGE on spinal myelination. Results: Pretreatment with KRGE (100 mg/kg, orally) for 10 days before immunization with myelin basic protein $(MBP)_{68-82}$ peptide exerted a protective effect against demyelination in the spinal cord, with inhibited recruitment and activation of immune cells including microglia, decreased mRNA expression of detrimental inflammatory mediators (interleukin-6, interferon-${\gamma}$, and cyclooxygenase-2), but increased mRNA expression of protective inflammatory mediators (insulin-like growth factor ${\beta}1$, transforming growth factor ${\beta}$, and vascular endothelial growth factor-1). These results were associated with significant downregulation of p38 mitogen-activated protein kinase and nuclear factor-${\kappa}B$ signaling pathways in microglia/macrophages, T cells, and astrocytes. Conclusion: Our findings suggest that KRGE alleviates spinal demyelination in acute experimental autoimmune encephalomyelitis through inhibiting the activation of the p38 mitogen-activated protein kinase/nuclear factor-${\kappa}B$ signaling pathway. Therefore, KRGE might be used as a new therapeutic for autoimmune disorders such as multiple sclerosis, although further investigation is needed.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 춘계학술발표회
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• pp.31-31
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• 2018

The purpose of this study was to statistically analyze amylose and protein content of rice variety resources collected from China (1,542), Japan (1,409), Korea (413), and India (287). The statistical analysis was conducted using ANOVA and DMRT based on the data obtained from NIRS analysis. The average amylose contents were 18.85% in Japanese, 19.99% in Korean, 20.27% in Chinese, and 25.46% in Indian resources. The average protein contents were 7.23% in Korean, 7.73% in Japanese, 8.01% in Chinese, and 8.17% in Indian resources. The amylose and protein content using ANOVA showed significant differences at the level of 0.01. The F-test for amylose content was 158.34, and for protein content was 53.95 compared to critical value 3.78. The amylose and protein content using DMRT (p<0.01) showed significant difference between countries. The value of statistical treatment was divided into three groups such as $China^a$, $Korea^a$, $Japan^b$, $India^c$ in amylose and $China^a$, $India^a$, $Japan^b$, $Korea^c$ in protein. Japanese resources had the lowest level of amylose contents, whereas, the lowest level of protein content was found in Korean resources compared to other origins. Indian resources showed the highest level of amylose and protein contents. It is recommended that these results could be helpful to future breeding experiments.

• 한국정보과학회논문지:시스템및이론
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• 제29권9호
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• pp.514-519
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• 2002

단백질의 구조를 예측하는 과정에 사용될 수 있는 다음 문제를 고려한다. 길이가 n이고 원소가 모두 양수인 두 배열 A, B와 양수 M이 주어질 때, A[i]＋…A[j]＋B[k]＋…B[ι]=M이 되는 부배열 쌍 A[i]＋…A[j],$1{\leq}i{\leq}j{\leq}n$과 B[k], …, B[l], $1{\leq}k{\leq}l{\leq}n$을 모두 찾으시오. 본 논문에서는 이 문제를 $Ο(n^2log n+K)$ 시간에 Ο(n) 메모리를 사용하여 해결하는 알고리즘을 제시한다. 단, K는 찾은 부배열 쌍의 수이다. 기존의 결과는$Ο(n^2log +Klog n)$ 시간과 Ο(n) 메모리였다.

• Tuberculosis and Respiratory Diseases
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• 제54권4호
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• pp.439-448
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• 2003

연구배경 : SP는 표면활성물질의 물리적 성상의 결정 및 대사를 결정하는데 있어서 중요한 역할을 담당한다. SP-B와 SP-C는 배수성 단백이며 공기액체계면에 지질 수포를 결합하게 하여 인지질의 흡착과 표면활성을 증강시킨다. 글루코코르티코이드는 폐의 형태학적 발생을 촉진시키며, 표면활성물질 인지질의 생산을 증가시키고, SP-B와 SP-C의 축적 및 폐탄성을 향상시킨다. 시험관 내 실험을 통하여 관찰한 바에 의하면 글루코코르티코이드를 투여한 후에 SP-B mRNA와 SP-C mRNA가 증가한다. 그러나 동물실험에서 SP-B mRNA와 SP-C mRNA에 대한 스테로이드제의 효과에 관한 보고는 드물다. 방 법 : 저자들은 실험동물에서 SP-B와 SP-C의 유전자 발현을 파악하고자 서로 다른 용량과 기간에 따른 덱사메타손을 백서에 투여한 후 이들 유전자 발현 양상을 filter hybridization방법으로, 덱사메타손의 서로 다른 용양과 기간에 따른 SP-B와 SP-C의 유전자 발현 효과를 평가하였다. 결 과 : 1) 덱사메타손을 1일 0.2 mg/kg 투여하고 24시간 경과 후 SP-B mRNA양은 대조군에 비하여 23.7%가 증가하였다. 2) 덱사메타손을 1일 2 mg/kg씩 일주일간 투여 후 SP-B mRNA양은 대조군에 비하여 96.6%가 증가하였다(P<0.001). 3) 덱사메타손을 1일 0.2 mg/kg 투여하고 24시간 경과 후 SP-C mRNA양은 대조군에 비하여 42.7%가 증가하였다(P<0.01). 4) 덱사메타손을 1일 2 mg/kg씩 1주일간 투여 후 SP-C mRNA양은 대조군에 비하여 60.0%가 증가하였다(P<0.01). 결 론 : 이상의 결과는 실험동물에서 서로 다른 덱사메타손용량과 기간에 따른 SP-B mRNA와 SP-C mRNA양은 덱사메타손양을 소량을 투여하였을 때보다 대량을 투여 하였을 때, 또는 단기간보다는 장기간 사용하였을 때 유의한 증가가 있음을 지적하고 있다.

• BMB Reports
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• 제32권6호
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• pp.521-528
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• 1999

The folding of recombinant hepatitis B virus X-protein (rHBx) solubilized from Escherichia coli inclusion bodies was investigated. By sequential dialysis of urea, rHBx was folded into its native structure, which was demonstrated by the efficacy of its transcriptional activation of the adenovirus major late promoter (MLP), fluorescence spectroscopy, and circular dichroism (CD) analysis. The decrease in CD values at 220 nm and a corresponding blue shift of the intrinsic fluorescence emission confirmed the ability of rHBx to refold in lower concentrations of urea, yielding the active protein. Equilibrium and kinetic studies of the refolding of rHBx were carried out by tryptophan fluorescence measurements. From the biphasic nature of the fluorescence curves, the existence of stable intermediate states in the renaturation process was inferred. Reverse phase-high performance liquid chromatography (RP-HPLC) analysis further demonstrated the existence of these intermediates and their apparent compactness.

• Asian Pacific Journal of Cancer Prevention
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• 제16권1호
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• pp.233-237
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• 2015

Background: Lung cancer is the leading cause of cancer death in Brunei Darussalam, accounting for almost 20% of the total. The epidermal growth factor receptor (EGFR) is a member of the erbB family of tyrosine kinase receptor proteins, which includes c-erbb2(HER2/neu), erb-B3, and erb-B4. EGFR overexpression is found in a third of all epithelial cancers, often associated with a poor prognosis. Materials and Methods: Protein expression of EGFR in 27 cases of lung cancer tissue samples and 9 cases of normal lung tissue samples was evaluated using an immunohistochemical approach. Results: The results demonstrated significant increase and overexpression of EGFR in Bruneian lung cancer tissue samples in comparison to normal lung tissue. However, there was no significant relationship between clinicopathologic variables (age and sex) of patients and EGFR protein expression. Conclusions: EGFR is overexpressed in Bruneian lung cancer patient tissue samples in comparison to normal lung tissue samples. This may indicate that EGFR protein over expression plays an important role in the genesis of this type of cancer in Brunei Darussalam.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.215-222
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• 2006

The Lowry method was used in this study to measure protein in Haemophilus influenzae type b (Hib) conjugate vaccines (polyribosylibitol phosphate-tetanus toxoid; PRP-TT) using deoxycholic acid (DOC) to induce protein precipitation. Trichloroacetic acid (TCA) did not induce precipitation adequately from the Hib conjugate bulk and the freeze-dried Hib conjugate product. Its yield was approximately 50%. The matrix structure of Hib conjugate inhibits precipitation by TCA. Although the Lowry method can be carried out without precipitation in Hib conjugate bulk when no residual impurities (adipic acid dihydrazide [ADH], 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide-HCI [EDAC], phenol and cyanogens bromide [CNBr], etc.) are present, it cannot be used for Hib conjugate products that contain sucrose 8.5%, because 8.5% concentration of sucrose enhanced the protein concentration. DOC- and HCl-induced precipitation is an alternative method for evaluating the protein content of the Hib conjugate bulk and the Hib conjugate product. The precipitation was optimal with a final concentrate of 0.1% for DOC at $4^{\circ}C$ and pH 2. This Lowry method, using DOC/HCI precipitation to induce protein precipitation, was confirmed a consistent, reproducible, and valid test for proteins in Hib conjugate bulk and its freeze-dried product.

• Tuberculosis and Respiratory Diseases
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• 제48권4호
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• pp.513-521
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• 2000

Background : TNF may play an important role(central mediator) in the development of an acute respiratory distress syndrome. Since TNF induced lung injury in the acute respiratory distress syndrome and abnormalities in surfactant function have been described in acute respiratory distress syndrome, the authors investigated the effects of TNF on the regulation of surfactant protein A, B and C mRNA accumulation. Methods : The effects of TNF on gene expression of surfactant protein A, B, and C were analyzed using filter hybridization, 12 and 24 hours after intravenous injection of TNF in rats. Results : 1. The accumulation of SP-A mRNA in the TNF treated group (12 and 24 hours after TNF injection) was significantly decreased by 22.9% and 27.4%, respectively, compared to the control group (P<.025, P<.025). 2. The accumulation of SP-B mRNA in 24 hours after TNF treated group was significantly decreased by 20.5% compared to that of the control group(P<.01). 3. The accumulation of SP-C mRNA in 12 hours after TNF treated group was significantly decreased by 31% the compared to that of the control group(P<.01). Conclusions : These findings indicate the marked inhibitory effects of tumor necrosis factor on surfactant proteins expression in vivo. This finding. in turn, supports the idea of inhibitory effects of tumor necrosis factor on surfactant proteins expression as it relates to pathogenesis of acute respiratory distress syndrome.

• Bulletin of the Korean Chemical Society
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• 제26권7호
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• pp.1138-1140
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• 2005

• 생명과학회지
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• 제27권6호
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• pp.673-679
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• 2017

미세소관을 따라 이동하는 모터단백질들은 세포내 물질수송에 필수적인 역할을 한다. Kinesin 1은 세포내에서 미세소관을 따라 움직이는 모터단백질로서 다양한 소포, mRNA, 그리고 단백질의 세포내 수송에 관여한다. Kinesin 1은 2개의 장쇄단위체(KHCs, 또는 KIF5s)와 2개의 경쇄단위체(KLCs)로 구성되어 있다. KIF5s는 N-말단에 모터도메인을 가지고 있고 C-말단의 운반체 결합도메인을 통해 다양한 운반체와 결합한다. 본 연구에서 KIF5B와 결합하는 단백질을 분리하기 위하여 효모 two-hybrid 탐색을 수행한 결과 미세소관의 plus end 결합단백질인 cytoplasmic linker protein 170 (CLIP-170)을 분리하였다. CLIP-170의 coiled-coil 도메인은 KIF5B의 운반체 결합도메인과 결합하였다. 또한 CLIP-170은 KIF5A와 KIF5C와도 결합하였다. 그리고 glutathione S-transferase (GST) pull-down을 통해 KIF5s와 CLIP-170이 단백질수준에서 결합함을 확인하였다. 생쥐 뇌파쇄액을 KIF5B 항체로 면역침강한 결과 CLIP-170이 같이 침강함을 확인하였다. 이러한 결과들은 kinesin 1이 세포내에서 CLIP-170을 운반함을 시사한다.