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Studies on Inhibition of Self-Incompatibility with Micronutrients in Apple (미량요소 첨가가 자가불화합성 억제에 미치는 영향)

  • Chung, Il-Kyung;Son, Tae-Kwon;Kim, Min-Ji
    • Korean Journal of Plant Resources
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    • v.25 no.2
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    • pp.285-292
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    • 2012
  • Self-incompatibility (SI) system is a genetic barrier that prevents self-fertilization and promotes cross-pollination among different S genotypes. In many of these species, SI is controlled by a single genetic locus known as S locus, which prevents the fertilization by pollen with same locus. S RNases are the products of the S-locus expressed in the stylar tissue of Fuji Apple with gametophytic self-incompatibility system. This study investigated the various types of chemicals in order to select more effective inhibitors and activators. The effect on the inhibition of S RNase of Fuji apples was investigated $in$ $vitro$. The result showed that the enzyme activity was reduced 24.3% by Iron(II) Sulfate, significantly. $In$ $vitro$ studies of pollen growth tube showed that pollen tube growth had a higher germination rate (90%) in 10% Sucrose than in 2% sucrose extension medium. Data on the fruit set of apples treated with inhibitor and activator. Double application of $A^+$(Apple Plus, ISTECH Co. Ltd.,)+Vitamin B6 had the highest central fruit set as 86.1%(Andong). One time application of $A^{++}$Vitamin B1 in Yeongju obtained the highest central fruit set (91.9%).

Aboveground and Soil Carbon Storages in Quercus mongolica and Quercus variabilis Natural Forest Ecosystems in Chungju (충주지역(忠州地域)의 신갈나무와 굴참나무 천연림(天然林) 생태계(生態系)의 지상부(地上部) 및 토양(土壤) 중(中) 탄소고정(炭素固定)에 관(關)한 연구(硏究))

  • Park, Gwan-Soo
    • Journal of Korean Society of Forest Science
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    • v.88 no.1
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    • pp.93-100
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    • 1999
  • This study has been carried out to estimate aboveground and soil carbon contents in an average 39-year-old Quercus mongolica and 40-year-old Quercus variabilis stands in Chungju, Chungbuk. Ten sample trees were cut in each forest and soil samples were collected. Aboveground carbon content was estimated by the equation model $Wt=aD^b$ where Wt is oven-dry weight in kg and D is DBH in cm. Total aboveground carbon content was 48.85tonC/ha in Quercus mongolica stand and 57.49tonC/ha in Quercus variabilis stand. The proportion of each tree component to total aboveground carbon content was high in order of bolewood, branches, bolebark, and leaves in the two forests. Aboveground net primary production was estimated at 5.88tonC/ha in Quercus mongolica stand and 5.12tonC/ha in Quercus variabilis stand. Soil carbon content was 67.0tonC/ha in Quercus mongolica stand, 67.8tonC/ha in Quercus variabilis stand, and 54.7tonC/ha in Pinus densiflora stand. There was no significant difference in soil carbon content among the three forests.

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Genetic Species Identification by Sequencing Analysis of Nuclear and Mitochondrial Genes for Albino Misgurnus Species from Korea (우리나라 미꾸리속(genus Misgurnus) 알비노 개체의 미토콘드리아 및 핵 유전자 염기서열 분석에 의한 유전적 동정)

  • Song, Ha-Youn;Moon, Shin-Joo;Kim, Keun-Sik;Bang, In-Chul
    • Korean Journal of Ichthyology
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    • v.29 no.2
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    • pp.139-145
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    • 2017
  • The spontaneous color mutant, albino individuals of genus Misgurnus, are rarely discovered in Korea and there are difficult to identify morphological species due to lack melanin pigmentation. In this study, we developed a genetic identification method for the species of albino Misgurnus individuals based on phylogenetic analysis by using recombination activating gene 1 (rag1) and cytochrome b (cytb) region of mitochondrial DNA. As a result of molecular phylogenetic analysis, three clades were identified as Misgurnus mizolepis, M. anguillicaudatus and M. mohoity. The homology of the cytb sequences of M. mohoity was best match to that of M. mohoity sequences in GenBank database. As a result of species identification of 25 albino Misgurnus individuals based on the phylogenetic tree, the red-eye type was identified as 16 M. anguillicaudatus and one M. mizolepis. The remaining three individuals were identified as one M. mizolepis ♀${\times}$M. anguillicaudatus ♂, and two M. mohoity ♀${\times}$M. anguillicaudatus ♂, respectively. In addition, the five black-eye type individuals were identified as one M. anguillicaudatus, three M. mizolepis and one M. mohoity. Therefore, this genetic identification method will be an useful techniques for species or hybrid identification in genus Misgurnus.

Spherical Pyramid-Technique : An Efficient Indexing Technique for Similarity Search in High-Dimensional Data (구형 피라미드 기법 : 고차원 데이터의 유사성 검색을 위한 효율적인 색인 기법)

  • Lee, Dong-Ho;Jeong, Jin-Wan;Kim, Hyeong-Ju
    • Journal of KIISE:Software and Applications
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    • v.26 no.11
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    • pp.1270-1281
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    • 1999
  • 피라미드 기법 1 은 d-차원의 공간을 2d개의 피라미드들로 분할하는 특별한 공간 분할 방식을 이용하여 고차원 데이타를 효율적으로 색인할 수 있는 새로운 색인 방법으로 제안되었다. 피라미드 기법은 고차원 사각형 형태의 영역 질의에는 효율적이나, 유사성 검색에 많이 사용되는 고차원 구형태의 영역 질의에는 비효율적인 면이 존재한다. 본 논문에서는 고차원 데이타를 많이 사용하는 유사성 검색에 효율적인 새로운 색인 기법으로 구형 피라미드 기법을 제안한다. 구형 피라미드 기법은 먼저 d-차원의 공간을 2d개의 구형 피라미드로 분할하고, 각 단일 구형 피라미드를 다시 구형태의 조각으로 분할하는 특별한 공간 분할 방법에 기반하고 있다. 이러한 공간 분할 방식은 피라미드 기법과 마찬가지로 d-차원 공간을 1-차원 공간으로 변환할 수 있다. 따라서, 변환된 1-차원 데이타를 다루기 위하여 B+-트리를 사용할 수 있다. 본 논문에서는 이렇게 분할된 공간에서 고차원 구형태의 영역 질의를 효율적으로 처리할 수 있는 알고리즘을 제안한다. 마지막으로, 인위적 데이타와 실제 데이타를 사용한 다양한 실험을 통하여 구형 피라미드 기법이 구형태의 영역 질의를 처리하는데 있어서 기존의 피라미드 기법보다 효율적임을 보인다.Abstract The Pyramid-Technique 1 was proposed as a new indexing method for high- dimensional data spaces using a special partitioning strategy that divides d-dimensional space into 2d pyramids. It is efficient for hypercube range query, but is not efficient for hypersphere range query which is frequently used in similarity search. In this paper, we propose the Spherical Pyramid-Technique, an efficient indexing method for similarity search in high-dimensional space. The Spherical Pyramid-Technique is based on a special partitioning strategy, which is to divide the d-dimensional data space first into 2d spherical pyramids, and then cut the single spherical pyramid into several spherical slices. This partition provides a transformation of d-dimensional space into 1-dimensional space as the Pyramid-Technique does. Thus, we are able to use a B+-tree to manage the transformed 1-dimensional data. We also propose the algorithm of processing hypersphere range query on the space partitioned by this partitioning strategy. Finally, we show that the Spherical Pyramid-Technique clearly outperforms the Pyramid-Technique in processing hypersphere range queries through various experiments using synthetic and real data.

Biomass and Net Primary Production of Quercus variabilis Natural Forest Ecosystems in Gongju, Pohang, and Yangyang Areas (공주(公州), 포항(浦港), 그리고 양양(襄陽) 지역(地域) 굴참나무 천연림(天然林) 생태계(生態系)의 물질생산(物質生産)에 관(關)한 연구(硏究))

  • Park, Gwan-Soo;Lee, Sung-Woo
    • Journal of Korean Society of Forest Science
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    • v.90 no.6
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    • pp.692-698
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    • 2001
  • This study has been carried out to estimate aboveground biomass and net primary production(NPP) in an average 41-years-old Quercus variabilis stand of Gongju area, 45-years-old Quercus variabilis stand of Pohang area, and 54-years-old Quercus variabilis stand of Yangyang area. Ten sample trees were cut in each forest and soil samples were collected in July to August, 2000. Estimation for aboveground biomass and net primary production were made by the equation model $Wt=aD^b$ where Wt is oven dry weight in kg and D is DBH in cm. Total aboveground biomass was 91.31ton/ha in Gongju area, 207.6ton/ha in Pohang area, and 71.39ton/ha in Yangyang area. The aboveground biomass 207.6ton/ha in Pohang area is the highest biomass production among the amount of biomass in Quercus variabils stands reported in Korea. The proportion of each tree component to total aboveground biomass was high in order of bolewood, bolebark, branches and leaves in the three forests. Aboveground total net primary production was estimated at 7.8ton/ha in Gongju area, 11.5ton/ha in Pohang area, and 6.40ton/ha in Yangyang area. There were at least 2 times higher total aboveground biomass in Pohang area than in the Gongju and Yangyang areas because of climate difference among the study areas.

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Effect of Light-emitting Diodes on Photosynthesis and Growth of in vitro Propagation in Tea Tree (Camellia sinensis L.) (LED 광질이 차나무 기내배양묘의 생육 및 광합성에 미치는 영향)

  • Im, Hyeon-Jeong;Na, Chae-Sun;Song, Chi-Hyeon;Won, Chang-O;Song, Ki-Seon;Hwang, Jung-Gyu;Kim, Do-Hyun;Kim, Sang-Geun;Kim, Hyun-Chul
    • Journal of agriculture & life science
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    • v.53 no.6
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    • pp.13-21
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    • 2019
  • The influences of light generated by LEDs on shoot growth and photosynthesis of Tea plant(Camellia sinensis L.) were evaluated. The growth characteristics were investigated after 45 days of culture under four different light qualities: fluorescent lamp, red LED, blue LED, red+blue+white LED. Shoot growth was promoted by red light, especially root length and area were further promoted under the red+blue+white LED. Also, T/R ratio and Chlorophyll content were highest in red+blue+white. Fluor Cam was used to measure the fluorescence images of the plants, inhibition of photochemical efficiency(Fv/Fm) were not changed in all treatment. However, non-photochemical quenching(NPQ) were found rapidly increasing in blue LED, these results were that blue LED were inhibit photosynthetic efficiency and must be considered for efficiently in vitro cultivation of the tea plant. The above results suggest that light qualities could be an important factor to foster in vitro growth of the species. Also, In order to produce healthy plants, it is effective to using light qualities of red+blue+white LED on in vitro culture of the tea plant. These results could be used to mass propagating shoot and produce of healthy seedling.

First Report of Fire Blight Caused by Erwinia amylovora on Korean Mountain Ash (Sorbus alnifolia) in Korea (Erwinia amylovora에 의한 팥배나무 화상병 발생 보고)

  • Yeon-Jeong Lim;Hyeonseok Oh;Mi-Hyun Lee;Eunjung Roh;Hyeonheui Ham;Dong Suk Park;Duck Hwan Park;Yong Hwan Lee
    • Research in Plant Disease
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    • v.29 no.1
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    • pp.79-81
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    • 2023
  • During the nationwide survey of fire blight, the typical shoot blight symptoms were found on Korean mountain ash (Sorbus alnifolia) which was located near an orchard that produced fire blight on pear trees in Eumseong, Korea, May 2021. To identify the causal agent, we progressed isolation from the symptomatic leaves and shoots. Two white and mucoid colonies were isolated into the pure culture. Two isolates were identified as Erwinia amylovora according to the colony-polymerase chain reaction (PCR) with amsB primers and the phylogenetic tree using 16S rRNA sequences. To test of pathogenicity of two isolates, we inoculated immature pear fruits and understock of apple. We observed necrosis and oozes on immature pear fruits and shoot blight resulting in necrosis on apple shoots six days after inoculation. Colonies were recovered from the inoculated pears and apples, and identity was confirmed through colony PCR for amsB genes. To our knowledge, E. amylovora was first reported on Korean mountain ash native to South Korea.

Biological activity of Euonymus alatus (Thunb.) Sieb. wing extracts (화살나무 날개 추출물의 생리활성)

  • Hye-Ji Min;Du-Hyun Kim;Kwon-Il Seo
    • Food Science and Preservation
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    • v.30 no.2
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    • pp.358-368
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    • 2023
  • Euonymus alatus (Thunb.) Sieb., also known as the arrow tree in Korea, is a plant in East Asia used in traditional medicine and food. In particular, the wings of E. alatus are rich in phenolic compounds. This study evaluated the antioxidant, α-glucosidase inhibition, and anti-cancer activities of E. alatus wing extracts. The radical and hydrogen peroxide scavenging acitvities and reducing the power of 1,000 ㎍/mL E. alatus wing extracts, were similar to those of the positive control (0.1% BHT, 0.1% α-tocopherol). In addition, ethanol and methanol extract at 250 ㎍/mL showed 95.70 and 94.99% of α-glucosidase inhibition activity, respectively. The ethanol extract of E. alatus wings had the highest total polyphenol and flavonoid contents (867.8 mg% and 521.7 mg%, respectively). The E. alatus wing extracts significantly decreased the cell viability of LNCaP human prostate cancer cells (p<0.001), MDA-MB-231 human breast cancer cells (p<0.001), and HT-29 human colon cancer cells (p<0.001) in a dose-dependent manner. However, there was no significant effect on B16 mouse melanoma cells. Notably, the ethanol extracts showed higher cancer cell growth inhibitory activity in LNCaP and HT-29 cells than the other extracts. These results suggest that E. alatus wing extracts could have significant clinical applications, and our results can be used as basic data for future functional food material development.

Growth-promoting effect of microorganisms from a fairy ring in Yangyang, Korea on Tricholoma matsutake mycelium (국내 양양 송이 자생지 내 균환 유래 토양미생물과 송이균사체 생장촉진 효과)

  • Doo-Ho Choi;Eunji Lee;Kang-Hyo Lee;Gi-Hong An
    • Journal of Mushroom
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    • v.22 no.1
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    • pp.22-26
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    • 2024
  • Tricholoma matsutake is a traditional favorite food in East Asia, cultivated in fairy rings called "shiro," which are found near Pinus densiflora. For effective artificial cultivation of Tri. matsutake, microorganisms from symbiotic fairy rings are co-cultivated. In this study, one bacterial isolate (Y22_B35) and two fungal isolates (Y22_F64 and Y22_F68) displayed growth-promoting effects on Tri. matsutake mycelium (158.47, 125.00, and 122.26% enhanced growth, respectively). For identification, 16S rRNA or ITS regions from the microorganisms¡¯ genomes were sequenced. Other sequences, including BenA, CaM, and RPB2 were sequenced in the fungal isolates. The bacterial isolate Y22_B35 was identified as Bacillus cereus. Y22_F64 and Y22_F68 were identified as Umbelopsis nana and Aspergillus parvulus, respectively. To identify the effects of the dominant microorganisms on Tri. Matsutake cultivation, metagenomic analyses were performed. Discovery of these Tri. matsutake mycelium growth-promoting microorganisms and metagenomics analyses are expected to contribute to our understanding of Tri. matsutake fruiting body growth and construction of biomimicry.

The Variation of Natural Population of Pinus densiflora S. et Z. in Korea (III) -Genetic Variation of the Progeny Originated from Mt. Chu-wang, An-Myon Island and Mt. O-Dae Populations- (소나무 천연집단(天然集團)의 변이(變異)에 관(關)한 연구(硏究)(III) -주왕산(周王山), 안면도(安眠島), 오대산(五臺山) 소나무집단(集團)의 차대(次代)의 유전변이(遺傳變異)-)

  • Yim, Kyong Bin;Kwon, Ki Won
    • Journal of Korean Society of Forest Science
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    • v.32 no.1
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    • pp.36-63
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    • 1976
  • The purpose of this study is to elucidate the genetic variation of the natural forest of Pinus densiflora. Three natural populations of the species, which are considered to be superior quality phenotypically, were selected. The locations and conditions of the populations are shown in table 1 and 2. The morphological traits of tree and needle and some other characteristics were presented already in our first report of this series in which population and family differences according to observed characteristics were statistically analyzed. Twenty trees were sampled from each populations, i.e., 60 trees in total. During the autumn of 1974, matured cones were collected from each tree and open-pollinated seeds were extracted in laboratory. Immediately after cone collection, in closed condition, the morphological characteristics were measured. Seed and seed-wing dimensions were also studied. In the spring of 1975, the seeds were sown in the experimental tree nursery located in Suweon. And in the April of 1976, the 1-0 seedlings were transplanted according to the predetermined experimental design, randomized block design with three replications. Because of cone setting condition. the number of family from which progenies were raised by populations were not equal. The numbers of family were 20 in population 1. 18 in population 2 and 15 in population 3. Then, each randomized block contained seedlings of 53 families from 3 populations. The present paper is mainly concerned with the variation of some characteristics of cone, seed, needle, growth performance of seedlings, and chlorophyll and monoterpene compositions of needles. The results obtained are summerized as follows. 1. The meteorological data obtained by averaging the records of 30 year period, observed from the nearest station to each location of populations, are shown in Fig. 3, 4, and 5. The distributional pattern of monthly precipitation are quite similar among locations. However, the precipitation density on population 2, Seosan area, during growing season is lower as compared to the other two populations. Population 1. Cheong-song area, and population 3, Pyong-chang area, are located in inland, but population 2 in the western seacoast. The differences on the average monthly air temperatures and the average monthly lowest temperatures among populations can hardly be found. 2. Available information on the each mother trees (families) studied, such as age, stem height, diameter at breast height, clear-bole-length, crown conditions and others are shown in table 6,7, and 8. 3. The measurements of fresh cone weight, length and the widest diameter of cone are given in Tab]e 9. All these traits arc concerned with the highly significant population differences and family differences within population. And the population difference was also found in the cone-index, that is, length-diameter ratio. 4. Seed-wing length and seed-wing width showed the population differences, and the family differences were also found in both characteristics. Not discussed in this paper, however, seed-wing colours and their shapes indicate the specificity which is inherent to individual trees as shown in photo 3 on page 50. The colour and shape are fully the expression of genetic make up of mother tree. The little variations on these traits are resulted from this reason. The significant differences among populations and among families were found in those characteristics, such as 1000-seed weight, seed length, seed width, and seed thickness as shown in table 11. As to all these dimensions, the values arc always larger in population 1 which is younger in age than that of the other two. The population differences evaluated by cone, seed and seed-wing sizes could partly be attributed to the growth vigorousity. 5. The values of correlation between the characteristics of cone and seed are presented in table 12. As shown, the positive correlations between cone diameter and seed-wing width were calculated in all populations studied. The correlation between seed-wing length and seed length was significantly positive in population 1 and 3 but not in population 2, that is, the r-value is so small as 0.002. in the latter. The correlation between cone length and seed-wing length was highly significant in population 1, but not in population 2. 6. Differences among progenies in growth performances, such as 1-0 and 1-1 seedling height and root collar diameter were highly singificant among populations as well as families within population(Table 13.) 7. The heritability values in narrow sense of population characteristics were estimated on the basis of variance components. The values based on seedling height at each age stage of 1-1 and 1-0 ranged from 0.146 to 0.288 and the values of root collar diameter from 0.060 to 0.130. (Table 14). These heritability values varied according to characteristics and seedling ages. Here what must be stated is that, for calculation of heritability values, the variance values of population was divided by the variance value of environment (error) and family and population. The present authors want to add the heritability values based on family level in the coming report. It might be considered that if the tree age is increased in furture, the heritability value is supposed to be altered or lowered. Examining the heritability values studied previously by many authors, in pine group at age of 7 to 15, the values of height growth ranged from 0.2 to 0.4 in general. The values we obtained are further below than these. 8. The correlation between seedling growth and seed characteristics were examined and the values resulted are shown in table 16. Contrary to our hypothetical premise of positive correlation between 1-0 seedling height and seed weight, non-significance on it was found. However, 1-0 seedling height correlated positively with seed length. And significant correlations between 1-0 and 1-1 seedling height are calculated. 9. The numbers of stomata row calculated separately by abaxial and adaxial side showed highly significant differences among populations, but not in serration density. On serration density, the differences among families within population were highly significant. (Table 17) A fact must be noted is that the correlation between stomata row on abaxial side and adaxial side was highly significant in all populations. Non-significances of correlation coefficient between progenies and parents regarding to stomata row on abaxial side were shown in all populations studied.(Table 18). 10. The contents of chhlorophyll b of the needle were a little more than that of chlorophyll a irrespective of the populations examined. The differences of chlorophyll a, b and a plus b contents were highly significant but not among families within populations as shown in table 20. The contents of chlorophyll a and b are presented by individual trees of each populations in table 21. 11. The occurrence of monoterpene components was examined by gas liquid chromatography (Shimazu, GC-1C type) to evaluate the population difference. There are some papers reporting the chemical geography of pines basing upon monoterpene composition. The number of populations studied here is not enough to state this problem. The kinds of monoterpene observed in needle were ${\alpha}$-pinene, camphene, ${\beta}$-pinene, myrcene, limonene, ${\beta}$-phellandrene and terpinolene plus two unknowns. In analysis of monoterpene composition, the number of sample trees varied with population, I.e., 18 families for population 1, 15 for population 2 and 11 for population3. (Table 22, 23 and 24). The histograms(Fig. 6) of 7 components of monoterpene by population show noticeably higher percentages of ${\alpha}$-pinene irrespective of population and ${\beta}$-phellandrene in the next order. The minor Pinus densiflora monoterpene composition of camphene, myrcene, limonene and terpinolene made up less than 10 percent of the portion in general. The average coefficients of variation of ${\alpha}$-pinene and ${\beta}$-phellandrene were 11 percent. On the contrary to this, the average coefficients of variation of camphene, limonene and terpinolene varied from 20 to 30 percent. And the significant differences between populaiton were observed only in myrcene and ${\beta}$-phellandrene. (Table 25).

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