• 미생물학회지
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• 제36권3호
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• pp.203-208
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• 2000

재조합 플라스미드 pAL850에 함유된 Bacillus circulans KCTC3004 $\alpha$-amylase 유 전자를 pUB110을 이용하여 shuttle 플라스미드 pALS111을 만들어 Bacillus 세포에 이동. 발현시켰다. Bacillus subtilis(고초균)와 Bacillus megaterium(거대균)으로 형질전환된 pALS111로부터 $\alpha$-amylase는 세포증식과 비례하여 생산되었다. 형질전화주가 생산하는 $\alpha$ -amylase의 최대활성을 유전자 공여 균주인 B. circulans와 비교했을 때 고초균은 약 95배, 거대균은 약 34배 정도의 높은 활성을 나타내었다. 그리고 대장균 형질전환주는 분비율이 10% 정도인데 반하여 고초균 형질전화주는 생산된 효소전부를 , 거대균 형질전환주는 약 98%를 세포외로 분비함을 보임으로써 고초균과 거대균은 실용적인 면에서 대장균보다 우월 함을 나타내었다, pALS111의 각 숙주 내에서의 안정성을 살펴본 결과 거대균에서는 92%, 고초균에서는 76%, 대장균에서는 38% 로 나타났다. SDS-PAGE와 zymogram을 통해 추정 된 대장균과 고초균, 거대균에서 발현된 효소의 분자량은 약 55,000으로 확인되었다. 이들 형질전환주가 생산하는 $\alpha$-amylase는 starch 에 작용하여 주된산물로서 maltotriose 이상의 다양한 maltooligosaccharide들을 생산함이 확인 되었다.

• 혜화의학회지
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• 제4권1호
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• pp.357-371
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• 1995

We have completed a study to measure the contents of glucose, BUN, creatinine. LDH, and T-protein with respect to a fatigued condition in the bloods of rats which a constant swimming is loaded and to measure the maximun swimming time of mice The test has been carried out as a part of the basic study on the efficacy of B. E. P. (Biological Energy Projector) for emitting a light energy having a specific wavelength out of far-infrared rays. As a result. We have reached the following conclusions: 1. At testing of mice's maximun swimming time, all of B.E.P.(2. 4. 8. 24hrs) treated group have been increased in comparison with the control group, but only 24hrs-B.E.P. treated group significantly increased during 4 weeks. 2. The contents of glucose, BUN. creatinine, LDH, and T-protein measured immediately after the swimming of mice have been distinctly changed but not been significantly changed at their increase and decrease in comparison with the control group. 3. At 3rd day out of the swimming loading, the contents of glucose in the blood serum of the white rat have been distinctly increased in comparison with the control group. And 24hrs-B.E.P treated group surpassed 8hrs-B.E.P. treated group. 4. At 1st and 3rd day, the contents of creatinine in the blood serum of the white rat have been distinctly increased at B.E.P. (8, 24hrs) treated groups in comparison with the control group and have been recovered to the condition of the normal group. 5. After three days, the contents of BUN in the blood serum of the white rat have been significantly decreased in B.E.P.(8, 24hrs) treated groups at 3rd day in comparison with the control group and have been recovered to the condition of the normal group. 6. The contents of LDH in the blood serum of the white rat have been decreased in B.E.P.(8, 24hrs) treated groups at 3rd day in comparison with the control group, in particular 24hrs-B.E.P. treated group has been decreased distinctly than the normal group. 7. The contents of T-protein in the blood serum of the white rat have been distinctly increased in B.E.P. (8, 24hrs) treated groups at 3rd day in comparison with the control and normal group. As the above results, it has been proved that the execise of mice and the fatigue metabolism of rats were influenced by the light energy emitted the B.E.P., and it has been also proved that the external stimulation could be used as a preferable stimulative factor for the biological metabolism. If the clinical training and study are positively achieved, the B.E.P. would be used as curative means and preventive measures for helping human body.

• Asian Pacific Journal of Cancer Prevention
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• 제15권5호
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• pp.2083-2087
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• 2014

Colorectal cancers remain to be a common cause of cancer-related death. Early-onset cases as well as those of various ethnic origins have aggressive clinical features, the basis of which requires further exploration. The aim of this work was to examine the expression patterns of $p15^{INK4b}$ and SMAD4 in colorectal carcinoma of different ethnic origins. Fifty-five sporadic colorectal carcinoma of Egyptian origin, 25 of which were early onset, and 54 cancers of Finnish origin were immunohistochemically stained with antibodies against $p15^{INK4b}$ and SMAD4 proteins. Data were compared to the methylation status of the $p15^{INK4b}$ gene promotor. $p15^{INK4b}$ was totally lost or deficient (lost in ${\geq}50%$ of tumor cell) in 47/55 (85%) tumors of Egyptian origin as compared to 6/50 (12%) tumors of Finnish origin (p=7e-15). In the Egyptian cases with $p15^{INK4b}$ loss and available $p15^{INK4b}$ promotor methylation status, 89% of cases which lost $p15^{INK4b}$ expression were associated with $p15^{INK4b}$ gene promotor hypermethylation. SMAD4 was lost or deficient in 25/54 (46%) tumors of Egyptian origin and 28/48 (58%) tumors of Finnish origin. 22/54 (41%) Egyptian tumors showed combined loss/deficiency of both $p15^{INK4b}$ and SMAD4, while $p15^{INK4b}$ was selectively lost/deficient with positive SMAD4 expression in 24/54 (44%) tumors. Loss of $p15^{INK4b}$ was associated with older age at presentation (>50 years) in the Egyptian tumors (p=0.04). These data show for the first time that $p15^{INK4b}$ loss of expression marks a subset of colorectal cancers and ethnic origin may play a role in this selection. In a substantial number of cases, the loss was independent of SMAD4 but rather associated with $p15^{INK4b}$ gene promotor hypermethylation and old age which could be related to different environmental exposures.

• Preventive Nutrition and Food Science
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• 제1권1호
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• pp.75-79
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• 1996

The purpose of this study was to investigate the effect of vitamin {TEX}$B_{6}${/TEX} deficiency on the utilization and recuperation of stored fuel in exercising rats. Thirty six rats were fed either a vitamin {TEX}$B_{6}${/TEX} deficient diet(-{TEX}$B_{6}${/TEX}) or a control diet(+{TEX}$B_{6}${/TEX}) for 5 weeks, then subdivided into 3 group: non-exercise group(NE), exercise group(EX), exercise and recuperation group(EX). EX group were exercised in treadmill({TEX}$10^{o}${/TEX}, 0.5~0.8km/h)for 2 hours and ER group were recuperated three days with the respective diet after exercise. Glucose(GLU), glycogen(GLY), protein(PRO), trigyceride(TG) and free fatty acid(FFA) were compared in plasma(P), liver(L) and skeletal muscle(M) of rats. Compared to +{TEX}$B_{6}${/TEX} rats, in NE group, the level of L-GLY of -{TEX}$B_{6}${/TEX} rats was higher, M-TG, L-PRO of -{TEX}$B_{6}${/TEX} rats were lower and there were no differences in P-PRO, P-FFA, P-TG, M-GLY, M-PRO and L-TG. In EX group, the levels of P-FFA, L-PRO of -{TEX}$B_{6}${/TEX} rats were higher. P-TG, L-TG of -{TEX}$B_{6}${/TEX} rats were lower and there was no difference in L-GLY. In EG group, the levels of P-GLU, P-PRO, P-RG, L-PRO of -{TEX}$B_{6}${/TEX} rats were lower and there were no differences in L-GLY, L-TG, M-TG and M-GLY. these results suggest that a lowered intake of vitamin {TEX}$B_{6}${/TEX} may impair the recuperation of aminals after exercise related to exercise fuel stores although there is a compensation among stored fuel utilization during exercise.

• 한국식품조리과학회지
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• 제9권4호
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• pp.323-328
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• 1993

유지를 가열할때 생성되는 B(a)P 및 기타 PAHs함량과 각 유지의 이화학적 성질변화와의 관계를 비교해 봄으로써 각 유지의 적절한 가열 온도와 시간을 알아보기 위하여 시판 대두유를 $180\pm$5^{\circ}C$$,$200\pm$5^{\circ}C$$, $300\pm$5^{\circ}C$$,에서 50시간 가열한 것을 시료로 취하였다. 이들 각 시료의 B(a)P 및 기타 PAHs함량은 HPLC/UV를 사용하여 측정하였고, 산패와 관련된 이화학적 성질변화를 알아보기 위하여 가열유지의 산값(Acid Value, AV)과, 공액이중 산값(Conjugated Diene Value, CDNV)을 측정하였다. 그 결과는 다음과 같았다. 1. 시료로 사용한 대두유의 B(a)P 및 PAHs함량은 Pyr 1.093, B(a)P 0.986, Ch 1.147, DMBA 1.082, B(e)P 0.664, Per 1.135, B(a)P 0.146, DBA 1.053, 3-MC 0.055 $\mu\textrm{g}$/kg 이었다. 대두유를 $180\pm$5^{\circ}C$$, 에서 10,20,30,50시간 가열했을 때 B(a)P함량은 각각 0.391, 0.692, 0.451, 0.372$\mu\textrm{g}$/kg이었으며 $200\pm$5^{\circ}C$$, 와 $300\pm$5^{\circ}C$$,에서 가열했을 때 B(a)P함량은 각각 10시간일때 0.844와 0.466, 20시간일때 0.512와 0.706, 30시간일때 0.479와 0.607, 50시간일때 0.367과 0.247 $\mu\textrm{g}$/kg이었다. 2. 대두유를 10,20,30,50시 간 가열했을 때 산값은 180$\pm$5$^{\circ}C200\pm$5^{\circ}C$$,에서는 0.26, 0.26, 0.27, 0.33이었고, 200$\pm$5$^{\circ}C$에서는 0.23, 0.21, 0.21, 0.52였으며 $300\pm$5^{\circ}C$$,에서는 0.47, 1.57, 3.90, 6.42였다. CDNV는 $180\pm$5^{\circ}C$$,에서는 0.67,0.76,0.99,1.04였으며, $200\pm$5^{\circ}C$$,$^{\circ}C$에서는 0.39, 0.49, 3.27, 3.89였고 $300\pm$5^{\circ}C$$,에서는 0.65, 2.I5, 3.00, 3.88이었다. 3. 대두유를 가열했을 때 B(a)P의 생성과 산패도의 변화와의 관계를 볼때 B(a)P함량은 $180^{\circ}C$에서는 20시간가열시 0.692$\mu\textrm{g}$/kg, $200\pm$5^{\circ}C$$,에서는 10시 간 가열시 0.844$\mu\textrm{g}$/kg, $300\pm$5^{\circ}C$$,에서는 20시간 가열시 0.706$\mu\textrm{g}$/kg으로 가장 높게 생성된 후 서서히 감소하였다. 그러나 산값과 공액이중산값은 계속 증가하는 양상을 나타냈다. 즉 B(a)P생성과 산패도 변화사이에는 일정한 관계를 나타내지는 않았다.

• Journal of Species Research
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• 제13권3호
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• pp.340-351
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• 2024

A new species of the canuellid genus Brianola Monard, 1926 (Copepoda: Harpacticoida) was collected during a meiofauna survey in July 2021 at the intertidal muddy sand of Phuket Island, Thailand. Currently, there are only eight known species of Brianola: B. stebleri, B. exigua, B. curvirostris, B. elegans, B. sydneyensis, B. vangoethemi, B. hamondi, and B. haliensis. Thus, the Brianola rawaiensis sp. nov. from Thailand is the ninth species of the genus. The new species, Brianola rawaiensis is most closely related to B. haliensis by sharing a four-segmented antennule in females and five-segmented antennule in males, eight-segmented antennary exopod and three-segmented antennary endopod, and P1 exp-3 with 5 spines/setae. However, B. rawaiensis sp. nov. is different from B. haliensis by having: 1) P1 exp-1 with 1 outer pinnate spine, 2) P1 exp-2 with inner plumose seta, 3) P2 exp-3 with 3 setae/spines, 4) P2 enp-1 with one inner plumose seta, 5) P4 enp-1 with inner seta. Sexual dimorphism is expressed in the antennule and segmentation of the urosome. Ovigerous female bearing one egg sac with 20 eggs.

• 동아시아식생활학회지
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• 제5권1호
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• pp.21-27
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• 1995

The protective effect of omija methanol extract on benzo(a)pyrene induce liver injury was studied in rats in vitro and in vivo. In vitro experiment, primary cultured hepatocytes(5${\times}$105cells/$m\ell$) were cultured for 20∼24 hours after adding omija methanol extract(5.1$\mu\textrm{g}$/$m\ell$) and B(a)P(50$\mu\textrm{m}$) in culture medium. In vivo experiment, omija methanol extract(0.1g/kg/day, per os) was administered for 7days and B(a)P(0.1mg/kg body weight, intraperitoneally) was given to the rats after the last administration of extract. Omija methanol extract significantly recovered serum enzyme activities(AST, ALT and LDH) and lipid contents(total cholesterol, triglyceride and HDL-cholesterol) changed by benzo(a)pyrene (B(a)P) to normal levels in vivo. In vitro experiment, as a result of 3-(4, 5-dimethlythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide(MTT) assay, omija methanol extract showed a little hepatotoxicity compared with group I (normal) but significantly recovered enzyme activities(AST, ALT and LDH) changed by B(a)P in comparison to group IIadministered B(a)P only. It was suggested that omija methanol extract has a protective effect on liver injury induced by B(a)P.

• 한국과학교육학회지
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• 제20권4호
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• pp.505-509
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• 2000

담화단위의 연관도를 측정하는 평균기본계수 $(\bar{B}_1)$, 평균가중계수 $(\bar{B}_2)$ 그리고 진행도 (P)는 다음과 같다. T1($\bar{B}_1$, 0.21: $\bar{B}_2$, 0.86: P. 0.79), T2($\bar{B}_1$, 0.02:$\bar{B}_2$, 0.85: P. 0.80), T3($\bar{B}_1$, 0.22: $\bar{B}_2$, 0.83: P.0.78) and T4($\bar{B}_1$, 0.14: $\bar{B}_2$, 0.82: P. 0.86), T4 교사는 다른 교사들에 비해 낮은 역동적 구조를 나타냈으나 수업은 빠른 진행도를 보였다. 총 담화단위 수, 어소의 총 빈도 수, 어소의 종류 수는 T1, T2, T3,T4 교사의 순으로 나타났다. 결과적으로 T4 교사는 다른 교사에 비해 수업 내용의 역동적 구조가 전체적으로 볼 때 낮은 수준을 보였는데, 그 주된 이유는 T4 교사는 수업 준비를 체계적으로 하지 않아, 수업내용이 상대적으로 적었기 때문으로 추측된다. 게다가 T4 교사가 담당하고 있는 학급이 1개 학급이기에 반복적인 수업이 이루어지지 않아 자기 분석의 기회가 없었고, 수업의 내용이 전공 영역과의 관련성이 적었으며 교직 경력 또한 다른 교사보다 낮았기 때문으로 사료된다.

• 대한수학회지
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• 제43권3호
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• pp.579-591
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• 2006

In this paper we obtain a sufficient and necessary condition for an analytic function f on the unit ball B with Hadamard gaps, that is, for $f(z)\;=\;{\sum}^{\infty}_{k=1}\;P_{nk}(z)$ (the homogeneous polynomial expansion of f) satisfying $n_{k+1}/n_{k}{\ge}{\lambda}>1$ for all $k\;{\in}\;N$, to belong to the weighted Bergman space $$A^p_{\alpha}(B)\;=\;\{f{\mid}{\int}_{B}{\mid}f(z){\mid}^{p}(1-{\mid}z{\mid}^2)^{\alpha}dV(z) < {\infty},\;f{\in}H(B)\}$$. We find a growth estimate for the integral mean $$\({\int}_{{\partial}B}{\mid}f(r{\zeta}){\mid}^pd{\sigma}({\zeta})\)^{1/p}$$, and an estimate for the point evaluations in this class of functions. Similar results on the mixed norm space $H_{p,q,{\alpha}$(B) and weighted Bergman space on polydisc $A^p_{^{\to}_{\alpha}}(U^n)$ are also given.

• 한국식품영양과학회지
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• 제30권2호
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• pp.320-324
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• 2001

To investigate the effect of Sarcodon aspratus methanol extract on liver damage in benzo(a)pyrene (B(a)P)-treated mice, mice were divided into 4 groups of control, B(a)P, Sarcodon aspratus methanol extract and Sarcodon aspratus methanol extract-B(a)P. The activities of serum aminotransferase, cytochrome P-450 and hepatic content of lipid peroxide after B(a)P-treatment were increased than control, but those levels were significantly decreased by the treatment of Sarcodon aspratus methanol extract. On the other hand, the hepatic glutathione content and glutathione S-transferase activity were increased by the treatment of Sarcodon aspratus methanol extract. Also the activities of superoxide dismutase, catalase and glutathione peroxidase were decreased by the treatment of Sarcodon aspratus methanol extract. In addition cytochrome P-450 1A1 isozyme protein level, which was remarkably increased by B(a)P treatment from results of immuno blotting, was decreased by the treatment with methanol extract of Sarcodon aspratus. These results suggest that Sarcodon aspratus methanol extract have protective effect on liver damage by decreasing lipid peroxide and activities of free radical generating enzymes.