Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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Effect of Omija(Schizandra chinensis Baillon) Methanal Extract on Benzo(a)pyrene induced Hepatotoxicity in Rats

오미자 메탄올 추출액이 흰쥐에 있어서 Benzo(a)pyrene에 이해 유도된 간장해에 미치는 영향

  • 이윤경 (영남대학교 식품영양학과)
  • Published : 1995.04.01
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Abstract

The protective effect of omija methanol extract on benzo(a)pyrene induce liver injury was studied in rats in vitro and in vivo. In vitro experiment, primary cultured hepatocytes(5${\times}$105cells/$m\ell$) were cultured for 20∼24 hours after adding omija methanol extract(5.1$\mu\textrm{g}$/$m\ell$) and B(a)P(50$\mu\textrm{m}$) in culture medium. In vivo experiment, omija methanol extract(0.1g/kg/day, per os) was administered for 7days and B(a)P(0.1mg/kg body weight, intraperitoneally) was given to the rats after the last administration of extract. Omija methanol extract significantly recovered serum enzyme activities(AST, ALT and LDH) and lipid contents(total cholesterol, triglyceride and HDL-cholesterol) changed by benzo(a)pyrene (B(a)P) to normal levels in vivo. In vitro experiment, as a result of 3-(4, 5-dimethlythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide(MTT) assay, omija methanol extract showed a little hepatotoxicity compared with group I (normal) but significantly recovered enzyme activities(AST, ALT and LDH) changed by B(a)P in comparison to group IIadministered B(a)P only. It was suggested that omija methanol extract has a protective effect on liver injury induced by B(a)P.

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References

  1. 한국식문화학회지 v.4 no.2 오미자의 부위별 유리당, 지질과 비휘발성 유기산 조성에 관한 연구 이정숙.;이성우.
  2. 한국식문화학회지 v.4 no.2 오미자의 부위별 일반성분과 무기질함량에 관한 연구 이정숙.;이미경.;이성우.
  3. 한국식문화학회지 v.4 no.2 오미자의 부위별 총 아미노산과 유리 아미노산 조성에 관한 연구 이정숙.;이성우.
  4. 한국식품과학회지 v.22 no.1 구기자, 당귀, 오미자, 오갈피 추출물의 유리당, 유리아미노산, 유기산 및 타닌의 조성 오상룡.;김성수.;민병용.;정도효.
  5. 한국식품과학회지 v.24 no.1 반응표면 방법에 의한 오미자 음료제조의 최적화 강찬규.;박재한.;백상봉.;진홍승.;이규순.
  6. 한국식문화학회지 v.5 no.2 오미자 열매의 물추출물이 알콜대사에 미치는 효과 이정숙.;이성우.
  7. Chem. Biol. Interaction v.41 Pharmacological properties of dibenzo(a,c)cyclooctene derivatives isolated from Fructus Schizandrae chinensis Ⅲ : Inhibitory effects on carbon tetrachloride to lipid peroxidation,metabolism and covalent binding of carbon tetrachloride to lipids Lui,K.T.;Lesca,P.
  8. 한국영양식량학회지 v.16 no.4 오미자 추출물이 alloxan 부하가토의 혈청성분에 미치는 영향 서화중.;이명렬.;황경숙.
  9. The J. Ame. Med. Asso. v.219 no.9 Viral hepatitis abroad Woodson,R.D.;Cahill,K.M.
  10. 환경위생연구 v.2 no.1 천연자원의 간기능 보호효과에 관한 연구 : Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 길경의 효과 윤수홍.;조수열.;한성호.
  11. 환경위생연구 v.2 no.1 천연자원의 간기능 보호효과에 관한연구 : Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 강활의 효과 윤수홍.;이송에.;박은주.;이주영.
  12. 동아시아식생활학회지 v.4 no.2 갈근 메탄을 엑기스가 흰쥐에 있어서 benzo(a)pyrene에 의해 유도된 간장해에 미치는 영향 이윤경.
  13. J. Biol. Chem. v.226 A simple method for the isolation and purification of total lipids from animal tissue Folch,J.;Mee,L.;Stanley,G.S.H.
  14. J.Immunol.Method v.65 Rapid colorimetric assay for cellular growth and survival : Application to prolifenration and cytotoxicity assays Mosmann,T.
  15. 한약학Ⅱ 육창수.;심재호.;류기욱.;김형근.;남준형.
  16. Planta Med. v.49 Assay method for antihepatotoxic activity using carbon tetrachloride-induced cytotoxicity in primary cultured hepatocytes Kiso,Y.;Tohkin,M.;Hikino,H.
  17. Planta Med. v.56 Tert-butylhydroperoxide induce injury in isolated rat hepatocytes: A model for studying antihepatotoxic crude drugs Michel,J.;Alain,R.;Hacques,F.;Francois.M.;Pierre,D.
  18. J. Natural Pro. v.46 no.6 Assay method for antihepatotoxic activity using galactosamine-induced cytotoxicity in primary cultured hepatocytes Kiso,Y.;Tohkin,M.;Hikino,H.
  19. Cancer v.44 Binding of benzo(a)pyrene and diben(a,h) anthracene to the Ah receptor in mouse and rat hepatic cytosis Okey,A.B.;Duba,A.W.;Volla,L.M.
  20. 독성학 길영민.
  21. Yakugaku Zasshi v.102 no.6 Pharmacologcal studies on Schizandra Fruits Ⅱ: Effects of constituents of Schizandra fruits on drugs induced hepatic damage inrats chi,H.;Harada,M.
  22. Planta Med. v.4 Mechanism of anti-hepatptpxic activity of wuweizisu C and gomisin A Kiso,Y.;Tohkin,M.;HIkino,H.;Ikeya,Y.;Taguchi,H.
  23. Planta Med. v.50 antihepatotoxic actions of lignans from Schizandra chinensis fruits Hikino,H.;Kiso,Y.;Taguchi,H.;Ikeya,Y.