• 전기의세계
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• 제28권8호
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• pp.66-71
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• 1979

This paper describes electrical analysis of the information processing of the nervous system. A general-purpose electrical neuronal model for simulating the electrical activity in a single nerve cell and in small groups of nerve cell has constructed. This model consists of two basic electronic modules to represent respectively a "cell body" and an "axon (with synapses)", together with various related appurtenances. The primary advantages of this method are; holistic view, actual physical representation of various electrical activities in a single nerve cell, display of the activity of all nerve cells flexibility with respect to network parameters. Moreover, this model can effectively help push forward our general ability to explore and conceptualize the electrical activity of interconnected networks of nerve cell behaving in concert. Also, this electronic module technique is the best of various means for this task of realistic representation of aggregates of neurons.gregates of neurons.

• Applied Microscopy
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• 제17권2호
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• pp.41-54
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• 1987

This experiment was performed to investigate the acute and chronic effects of lead on cerebral cortex. In acute treatment, mouse were injected with lead acetate at dose of 0.3 mmole/kg body weight, and in chronic treatment, mouse were supplied 0.03 M lead acetate sol. in the place of water. After treatment, mouse were sacrificed at time intervals of 24, 48, 72, and 96 hours in acute treatment and at time intervals of 4 weeks and 8 weeks in chronic treatment. In acute treatment, acetylcholinesterase activity is reduced at 72 hours and recovered at 96 hours in homogenate, and reduced at 24 hours and recovered at 72 hours in crude synaptosomes. In chronic treatment, acetylcholinesterase activity is increased in young mouse but reduced in mother mouse. Ultrastructural changes were composed of swelling of Golgi apparatus, nerve terminals with diminished synaptic vesicles, and vacuolated myeline lamellae of myelinated axon.

• Molecules and Cells
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• 제43권10호
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• pp.880-888
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• 2020

Inherited peripheral neuropathy is a heterogeneous group of peripheral neurodegenerative disorders including Charcot-Marie-Tooth disease. Many peripheral neuropathies often accompany impaired axonal construction and function. To study the molecular and cellular basis of axon-defective peripheral neuropathy, we explore the possibility of using Caenorhabditis elegans, a powerful nematode model equipped with a variety of genetics and imaging tools. In search of potential candidates of C. elegans peripheral neuropathy models, we monitored the movement and the body posture patterns of 26 C. elegans strains with disruption of genes associated with various peripheral neuropathies and compiled a database of their phenotypes. Our assay showed that movement features of the worms with mutations in HSPB1, MFN2, DYNC1H1, and KIF1B human homologues are significantly different from the control strain, suggesting they are viable candidates for C. elegans peripheral neuropathy models.

• 대한의용생체공학회:의공학회지
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• 제2권1호
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• pp.3-14
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• 1981

A theoretical investigation of the origin of the spontaneous firing in the squid axon was done with a mathmatical computer modelling based on the Hodgkin-Huxley equation. It is strongly believed that the existence of calcium ions in the membrane is essential to cause firing. The results of the computer simulation of the modelling indicate that the sites of calcium ions in the membrane might be near the potassium channel and the leakage channel plays an important role in the firing. The orientation of the future research project was suggested. And also, a theoretical investigation of the origin of the firing in the propagating action potential was done in the same way.

• BMB Reports
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• 제42권1호
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• pp.1-5
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• 2009

Synaptic vesicles (SVs) are key structures for synaptic transmission in neurons. Numerous membrane-associated proteins are sorted from the Golgi complex to the axon and the presynaptic terminal. Protein-protein and protein-lipid interactions are involved with SV targeting in neurons. Interestingly, many SV proteins have lipid binding capability, primarily with either cholesterol or phosphoinositides (PIs). As examples, the major SV protein synaptophysin can bind to cholesterol, a major lipid component in SVs, while several other SV proteins, including synaptotagmin, can bind to PIs. Thus, lipid-protein binding plays a key role for the SV targeting of synaptic proteins. In addition, numerous SV proteins can be palmitoylated. Palmitoylation is thought to be another synaptic targeting signal. Here, we briefly describe the relationship between lipid binding and SV targeting.

• BMB Reports
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• 제50권1호
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• pp.5-11
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• 2017

One of the characteristics of the neurons that distinguishes them from other cells is their complex and polarized structure consisting of dendrites, cell body, and axon. The complexity and diversity of dendrites are particularly well recognized, and accumulating evidences suggest that the alterations in the dendrite structure are associated with many neurodegenerative diseases. Given the importance of the proper dendritic structures for neuronal functions, the dendrite pathology appears to have crucial contribution to the pathogenesis of neurodegenerative diseases. Nonetheless, the cellular and molecular basis of dendritic changes in the neurodegenerative diseases remains largely elusive. Previous studies in normal condition have revealed that several cellular components, such as local cytoskeletal structures and organelles located locally in dendrites, play crucial roles in dendrite growth. By reviewing what has been unveiled to date regarding dendrite growth in terms of these local cellular components, we aim to provide an insight to categorize the potential cellular basis that can be applied to the dendrite pathology manifested in many neurodegenerative diseases.

• The Korean Journal of Physiology and Pharmacology
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• 제7권3호
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• pp.175-179
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• 2003

Coptis chinensis (CC) is one of the traditional herbs used in Oriental medicine for the treatment of gastrointestinal disorders, anxiety, and insomnia. In this study, neurotrophic and neuritogenic effects of CC on rat pheochromocytoma (PC12) cells were evaluated. Pretreatment of PC12 cells with water extracts of CC $(120{\mu}g/ml)$ produced considerable outgrowth of neurites that is comparable to the effect of nerve growth factor (NGF). Therefore, neurite outgrowth was quantified and expression of NGF mRNA was examined. Furthermore, characteristics of neurites were immunocytochemically confirmed using axon and dendrite-specific antibodies. These results suggest that water extracts of CC contain components that have neurotrophic and neuritogenic properties.

• Journal of Trauma and Injury
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• 제26권3호
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• pp.63-73
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• 2013

Spinal cord injury (SCI) is a serious condition associated with social and familial burden, as well as significant neurologic deficit. Despite the many advances in the treatment of spinal cord injury, a fundamental treatment for neurologic functional recovery has not yet been developed. In this article, we review two directions of development for spinal cord injury treatment: neuroprotective pharmacological agents and axon-regenerating cell therapy. We expect developments in these two to lead to improve functional recovery in patients with spinal cord injuries and to reduce burdens on society, as well as the patients' families.

• Molecules and Cells
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• 제39권12호
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• pp.841-846
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• 2016

Local protein synthesis mediates precise spatio-temporal regulation of gene expression for neuronal functions such as long-term plasticity, axon guidance and regeneration. To reveal the underlying mechanisms of local translation, it is crucial to understand mRNA transport, localization and translation in live neurons. Among various techniques for mRNA analysis, fluorescence microscopy has been widely used as the most direct method to study localization of mRNA. Live-cell imaging of single RNA molecules is particularly advantageous to dissect the highly heterogeneous and dynamic nature of messenger ribonucleoprotein (mRNP) complexes in neurons. Here, we review recent advances in the study of mRNA localization and translation in live neurons using novel techniques for single-RNA imaging.

• Molecules and Cells
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• 제42권12호
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• pp.828-835
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• 2019

PIWI Argonaute proteins and Piwi-interacting RNAs (piRNAs) are expressed in all animal species and play a critical role in cellular defense by inhibiting the activation of transposable elements in the germline. Recently, new evidence suggests that PIWI proteins and piRNAs also play important roles in various somatic tissues, including neurons. This review summarizes the neuronal functions of the PIWI-piRNA pathway in multiple animal species, including their involvement in axon regeneration, behavior, memory formation, and transgenerational epigenetic inheritance of adaptive memory. This review also discusses the consequences of dysregulation of neuronal PIWI-piRNA pathways in certain neurological disorders, including neurodevelopmental and neurodegenerative diseases. A full understanding of neuronal PIWI-piRNA pathways will ultimately provide novel insights into small RNA biology and could potentially provide precise targets for therapeutic applications.