• 한국식품영양과학회지
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• 제27권4호
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• pp.712-717
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• 1998

This study was undertaken to investigate the inhibition effects of Auricularia auricula-judae methanol extract in edible mushroom on lipid peroxidation and liver damage in benzo(a)pyrene(B(a)P)-treated mice. The activities of serum aminotransferase, cytochrome P-450, superoxide dismutase, catalase, glutathione peroxidase and the hepatic content of lipid peroxide after B(a)P-treatment was markedly increased than control but those levels were significantly decreased by the treatment of Auricularia auricula-judae methanol extract. Glutathione S-transferase activity and the hepatic glutathione content were decreased by B(a)P-treatment than control, but those were also inhibited by the treament of Auricularia auricula-judae methanol extract. These results suggest that Auricularia auricula-judae methanol extract have a protective effect on liver damage by B(a)P.

• Mycobiology
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• 제51권2호
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• pp.109-113
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• 2023

Auricularia is one of the broadly cultivated edible mushrooms in Korea. Most of the Korean Auricularia strains used for cultivation and breeding are known as A. auricula-judae. Recently, this species has been reported to belong to a species complex. Therefore, this study was carried out to genetically clarify the bred and cultivated Korean A. auricula-judae strains. The internal transcribed spacer (ITS) and IGS1 rDNA region sequences were determined from 10 A. auricula-judae strains by PCR and sequencing. Variation in the nucleotide sequence and sequence length of the two rDNA regions were found among the seven A. auricula-judae strains. A maximum-likelihood (ML) phylogenetic tree based on the ITS sequences clearly placed all the 10 Korean A. auricula-judae strains in the A. heimuer clade of the A. auriculajudae complex. A. heimuer is diverged from A. auricula-judae. An ML phylogenetic tree based on the IGS1 sequences revealed the close relationship between Korean A. heimuer strains to Chinese A. heimuer strains. But each strain could be distinguishable by the IGS1 sequence. Furthermore, progeny strains in the seven Korean strains could be differentiated from their parental strains by the IGS1 sequence based phylogenetic tree. Our results are expected to be used to complement the distinction of domestic Auricularia cultivars.

• 한국버섯학회지
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• 제10권1호
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• pp.29-36
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• 2012

목이버섯 3품종의 영양성분을 분석한 결과 유리당은 4종의 성분이 검출되었고, 그 중 trehalose는 $11.56{\pm}0.59$에서 $27.17{\pm}0.27mg/g$로 검출된 당 중에서 가장 많이 함유하고 있는 것으로 나타났으며 갈색목이가 가장 높은 것으로 나타났다. 유기산은 7종, 아미노산은 24종의 성분이 분석되었으며, 전체 함량에서 모두 흑목이가 가장 높게 나타났다. 그리고 유기산과 아미노산 성분 중에서 감칠맛을 내는 성분인 citric acid 및 glutamic acid, asparagine 등의 함량 또한 흑목이에서 가장 높은 것으로 나타나 천연 조미재료로서의 가능성을 제시하였다. 비타민$D_2$함량은 $1.66{\pm}0.1$에서 $5.43{\pm}0.08{\mu}g/g$으로 다른 버섯류에 비해 높게 나타났다. 식이섬유에는 3품종 모두 60% 정도의 함량을 나타냈으며, 품종 간 유의적인 차이는 없었다. ${\beta}$-glucan 함량은 갈색목이가 $25.21{\pm}0.37%$로 털목이나 흑목이에 비해 높은 수준으로 나타났으며 항산화 성분인 총 폴리페놀 함량은 털목이, 갈색목이, 흑목이 순으로 높게 나타났다.

• Toxicological Research
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• 제27권1호
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• pp.11-14
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• 2011

The present study investigated the anti-inflammatory effects of dichloromethane extract of Auricularia auricula-judae. Dichloromethane extract of Auricularia auricula-judae inhibited Lipopolysaccharide (LPS)-induced nitric oxide (NO) production significantly in a dose-dependent manner in the concentration ${\geq}\;10\;{\mu}g/ml$ (p < 0.05). Furthermore, RT-PCR results of this study indicated that the extract markedly reduced the expressions of inflammatory cytokines (IL-6, TNF-$\alpha$ and IL-$1{\beta}$) mRNA in LPS-treated murine RAW 264.7 macrophages, which could possibly ameliorate the inflammation. Nevertheless, dichloromethane extract of Auricularia auricula-judae did not show complete inhibition of IL-6 mRNA expression. The inhibition of IL-$1{\beta}$ cytokine at protein level was also observed in a dose dependent manner. In conclusion, the current study revealed the previously unknown effect of dichloromethane ethyl extract of Auricularia auricula-judae inhibitions of the production of NO, IL-6, TNF-$\alpha$ and IL-$1{\beta}$ in LPS-stimulated macrophages.

• 한국균학회지
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• 제11권1호
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• pp.1-7
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• 1983

진탕배양에서 느타리와 목이의 균사체(菌絲體) 생산(生産)에 대한 실험(實驗)의 결과(結果)를 요약(要約)하면 다음과 같다. 1. 균사체생산(菌絲體生産)에 양호(良好)한 탄소원(炭素源)은 P. ostreatus경우 glucose와 mannitol이었고 A. auricula-judae는 mannitol과 fructose이었다. 유기산(有機酸)중에서는 양균주(兩菌株) 모두 fumaric acid에서 제일 양호(良好)하였다. 2. 균사체생산(菌絲體生産)에 양호(良好)한 질소원(窒素源)은 P. ostreatus 경우 peptone, urea와 asparagin, DL-threonine이 었고 A. auricula-judae는 peptone, Casamino acid와 L-Serine, DL-threonine이었다. 3. 균사체생산(菌絲體生産)에 양호(良好)한 vitamin은 A. auricula-judae 경우 folic acid, thiamine이었고 A. auricula-judae는 folic acid, inositol, riboflavin이었다. 4.양균주(兩菌株)가 $MgSO_4$는 0.08%, $KH_2PO_4$ 0.2%에서 균사체생산(菌絲體生産)이 양호(良好)하였다. 5. 균사체생산(菌絲體生産)의 최적온도(最適溫度)와 pH는 P. ostreatus에서 $25{\sim}30^{\circ}C$, $pH\;5.5{\sim}6.0$이고 A. auricula-judae에서 $25{\sim}30^{\circ}C$, $pH6.0{\sim}7.0$이었다.

• 한국균학회지
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• 제38권1호
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• pp.5-7
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• 2010

Auricularia auricula-judae is an edible mushroom, which is known as wood ear, free ear, black ear mushroom, and free jelly fish. Present experiments were conducted to determine the possibility of artificial cultivation with oak sawdust of A. auricula-judae. The duration of mycelial growth and days of pinhead formation on oak sawdust bag (1.4 kg) were 29~32 days and 13~17 days, respectively. The yield of fresh fruit-body was 275~350 g.

• 한국버섯학회지
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• 제12권2호
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• pp.88-95
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• 2014

Auricularia auricula-judae is an edible mushroom, which is known as wood ear, free ear, black ear mushroom, and free jelly fish. This study was carried out to obtain the basic information for mycelial culture conditions of Auricularia auriculajudae. According to colony diameter and mycelial density, the media for suitable mycelial growth were PDA and MCM. The optimum temperature for mycelial growth was $25{\sim}30^{\circ}C$. Carbon and nitrogen sources were mannose and malt extract, respectively. The optimum C/N ratio was in the range of 10 to 1 with 2% glucose. Other minor components for the optimal growth were thiamine-HCl and biotin as vitamins, succinic acid and lactic acid as organic acids, and $MgSO_4{\cdot}7H_2O$ and $KH_2PO_4$ as mineral salts.

• Mycobiology
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• 제38권1호
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• pp.74-77
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• 2010

To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from $15\sim35^{\circ}C$. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at $25^{\circ}C$ for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.

• 한국균학회지
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• 제6권2호
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• pp.25-30
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• 1978

새로운 야생식용(野生食用)버섯의 인공재배(人工載培) 가능성(可能性)을 검토(檢討)하고자 조사(調査)한 결과(結果), 1. 배지(培地) 종류별(種類別) 균멱생장(菌生長)은CSA 배지(培地)에서 Coprinus comatus 양호(良好)하였으나 Pleurotus cornucopiae, Auricularia auricula-judae 및 collybia sp는 불량(不良)하였다. 2. Malt extract의 첨가량(添加量)은 5g까지 증가(增加)시켰을 때 Lepista nuda와 Auricularia auricula-judae는 균멱생장(菌生長) 양호(良好)하였으나 Pholiota squarrosa는 불량(不良)하였다. 3. 균주별(菌株別) 온도(溫度)에 따른 균멱생장(菌生長)에 있어서 Lepiota procera $20{\sim}30^{\circ}C$, Pholiota squarrosa는 $20{\sim}25^{\circ}C$에서 양호(良好)하였다. 그 외 다른 균주(菌株)는 25^{\circ}C$에서 균먹(菌)가 양호(良好)했다. 4. 종균조시(種菌造時) Pholiota squarrosa는 참나무톱밥, Pleurotus cornucopiae는 활엽수토밥, Coprinus comatus는 양송이퇴비(堆肥)에서 균멱생장(菌生長)이 양호(良好)하였다. 5. 배지재두별(培地材枓別) 버섯발생(發生)은 볏짚배지(培地)에서 Coprinus comatus, Lepiota, alborubescens, 활엽수톱밥에서는 Auricularia auricula-judae, Pleurotus cornucopiae, 그리고 참나무톱밥에서 Pholiota squarrosa의 자실체(子實體)가 발생(發生)되어 인공재배(人工栽培)가 가능(可能)하였다.

• 한국버섯학회지
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• 제16권2호
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• pp.103-110
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• 2018

11 종의 목이버섯 자실체로부터 얻은 50% EtOH추출물을 사용하여 지방전구세포인 3T3-L1의 지방세포 분화억제 효과를 확인한 모든 strain에서 억제효과가 존재하였다. 털목이에서는 21001 균주가 가장 우수하였고 흑목이에서는 21002 균주가 억제효과가 우수하였다. 전반적으로 털목이가 흑목이에 비해 지방세포 분화억제 효과가 우수하였다. 또한 Adipocyte RNA 발현량과 protein 발현량 측정 실험에서도 털목이 21001 균주가 지방세포 분화억제 효과에 있어서 가장 우수하였다. 털목이 21001 버섯을 사용하여 동물실험을 진행한 결과 털목이 자실체 분말이나 50% EtOH 추출물을 급여한 실험에서 체중 감소효과가 나타났으며, 특히 EtOH 추출물 0.2%를 급여한 군에서 유의하게 감소함을 확인하였다(*P<0.05). 동물 희생 후 적출한 간 중량 및 부고환지방량과 혈청 내 중성지방 및 총 콜레스테롤, 혈당, ALT, AST 등도 털목이버섯 21001 EtOH 추출물 0.1% 이상 급여 시 고지방식이만을 급여한 군에 비해 유의차(*P<0.05 이상) 있게 감소함을 확인하였다.