• 한국약용작물학회지
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• 제23권2호
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• pp.161-167
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• 2015

This study was carried out to determine the effects of light controls and leaf mold on root growth and physiological responses of Atractylodes japonica growing in forest farming. The experiment was performed by light controls (100%, 62.5%, 40.3% and 19.7% of full sunlight) and application of leaf mold to soil. Height, stem diameter, number of flower buds and root collar diameter were the highest in leaf mold within 62.5% of full sunlight (relative light intensity 62.5%). And these were the higher in leaf mold within each light level. As the shading level increased, light saturation point and maximum photosynthesis rate decreased. As the light level decreased, SPAD value increased in control and leaf mold. As a result of surveying the whole experiment, A. japonica was judged worse root growth under the lower light level. It was concluded that the light level was one of the most important factors to produce A. japonica. Also, producing high-quality of A. japonica with the price competitiveness by using leaf mold like the experiment can be an effective way to increase incomes for farmers.

• Archives of Pharmacal Research
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• 제28권8호
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• pp.902-908
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• 2005

An oriental herbal combination (BDX-1) was isolated from Achyranthes bidentata and Atractylodes japonica. We previously tested the clinical effectiveness of BDX-1 in rheumatoid arthritis (RA) patients and found that it has a beneficial therapeutic effect. Here, we provide experimental evidence for the effectiveness of BDX-1 on RA in murine models. The oral administration of BDX-1 was found to markedly inhibit collagen-induced arthritis, adjuvant-induced arthritis, and zymosan-induced inflammation. It also inhibited carrageenan-induced acute edema and acetic acid-induced writhing response. In addition, the biological activity of BDX-1 was found to be strongly increased by fermentation. Our results suggest that BDX-1 could be useful for the treatment of rheumatoid arthritis.

• 한국약용작물학회지
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• 제26권3호
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• pp.220-226
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• 2018

Background: Atractylodes japonica Koidz. and Atractylodes macrocephala Koidz. belong to the family (Asteraceae). Their rhizomes, called white Atractylodes rhizomes, are used in traditional medicine. To address some issues with their cultivation, we recently developed eight hybrid cultivars by interspecific hybridization of A. japonica and A. macrocephala. This study was conducted to screen the hybrid cultivars that have high amounts of active ingredients and yield ability. Methods and Results: Experiments were conducted using the eight hybrid cultivars and A. macrocephala (control cultivar) in the experimental field of the department of Herbal Crop Research located in Eumseong, South Korea. We investigated the growth characteristics of the aerial and underground parts. Among the cultivars, 'Sanwon' had the highest rhizome dry weight (53.8 g/plant), followed by 'Dachul' (50.0 g/plant). In addition, the content of atractylenolide I, II, III and total active ingredients were investigated using high-performance liquid chromatography. Compared with A. macrocephala, most of the inter-specific hybrid cultivars had a higher content of active ingredients and yield ability. Conclusions: Through study, we established the superior quality of Atractylodes inter-specific hybrid cultivars. In particularly, it was found that 'Dachul' may be grown as a superior cultivar, with high amount of active ingredients as well as yield ability.

• 대한약침학회지
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• 제7권1호
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• pp.87-100
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• 2004

Objective : This research was performed to investigate the effect of herbal acupuncture(Atratylodes japonica, Coix lachrymajobi, Ephedra sinica, Atratylodes japonica mixed with Coix lachrymajobi and Ephedra sinica mixed with Green tea) at Pungnyung(ST40) and Umnungchon(SP9) on weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and HDL to total cholesterol ratio of rats fed high fat diet for 5weeks. Method : Experimental groups were divided into normal group(Normal), high fat diet group(Control), high fat diet and Atractylodes japonica-herbal acupuncture group(AJ), high fat diet and Coix lachrymajobi-herbal acupuncture group(CL), high fat diet and Ephedra sinica-herbaI acupuncture group(ES), high fat diet and Atractylodes japonica+Coix lachrymajobi-herbal acupuncture group(AJ+CL), Ephedra sinica+Green tea-herbal acupuncture group(ES+GT). Herbal acupuncture was bilaterally treated at the level of 132.5mg/kg body weight per 2day. Results : Body weight and food efficiency were decreased in AJ, ES, AJ+CL, ES+GT. The level of serum total cholesterol, triglyceride and free fatty acid were increased in AJ, ES, ES+GT. That of serum HDL-cholesterol was increased in AJ. The change of food intake, the level of serum phospholipid and ALP were not significant. The HDL to Total cholesterol ratio was increased in AJ and ES. Conclusion : Atractylodes japonica-herbal acupuncture in ST40 SP9 is effective on Body weight, food efficiency ratio, the level of serum lipid, protection of liver function and prevention cardiovascular risk by obesity induced by high fat diet. Herbal acupuncture mixed Ephedra sinica with Green tea can control the body weight, food efficiency ratio and the level of serum lipid.

• 한국약용작물학회지
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• 제27권6호
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• pp.404-411
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• 2019

Background: In September 2017, wilting and rhizome rot symptoms were observed on Atractylodes macrocephala Koidz. in Jecheon-si and Eumseong-gun. This study was carried out to isolate hitherto unidentified pathogenic fungi from A. macrocephala and to test the pathogenicity of isolated fungi against Atractylodes spp. genus such as A. macrocephala, A. japonica, and their interspecific hybrids. Methods and Results: The diseased plants were washed with running tap water, and the boundary between the healthy area and the diseased area was cut while the pathogens were isolated by growing cultures from the diseased areas on Phytophthora semi-selective medium. The internal transcribed spacer (ITS) region of the isolates was used in this study for identification. Test plants were cultivated in the glasshouse at 20℃ - 30℃ for 4 months and then used for pathogenicity test. The pots with plants inoculated with mycelial plugs and zoospores were placed at 25℃ for 48 h in a dew chamber where relative humidity was above 95%, and then moved into the glasshouse at 20℃ - 30℃. The presence or absence of pathogenicity of the strains was determined by evaluating the symptom of plant wilting. The inoculation test was performed in three replicates with a non-treated control. Conclusions: On the basis of results of ITS sequence analysis, the strains isolated from the diseased plants was identified as Phytophthora sansomeana. Biological assay using test plants confirmed the pathogenicity of P. sansomeana against Atractylodes macrocephala. This is the first report of rhizome rot in A. macrocephala caused by P. sansomeana.

• Journal of Applied Biological Chemistry
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• 제65권1호
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• pp.17-21
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• 2022

생약 중 잔류농약의 안전성을 확보하기 위해 metalaxyl에 대해 백출과 우슬의 잔류농약 분석법을 개발하고자 하였다. LC-MS/MS를 사용하여 QuEChERS법을 기반으로 백출과 우슬 중 metalaxyl을 분석하기 위하여 물로 습윤화 시켜 acetonitirile로 추출하여 MgSO₄와 NaCl을 첨가해 층 분리 후 SPE-NH₂ cartridge로 정제하였다. 본 분석법의 기기정량한계와 분석정량 한계는 0.002 ㎍/mL과 0.01 mg/kg이었고 표준검량선은 0.001-0.05 mg/L 범위에서 결정계수(R²) 0.999의 직선성을 확인하였다. 백출과 우슬에 대한 회수율은 MLOQ 수준, MLOQ의 10배 수준에서 88.1-109.1%의 우수한 회수율을 보였고, 변이계수는 2.0-6.2%로 10%이하였다. 본 연구에서 확립한 LC-MS/MS를 이용한 잔류분석법의 회수율 및 분석오차는 국제적인 기준을 만족하고 있으므로 생약 백출 및 우슬 중 metalaxyl의 안전성 검사를 위한 분석법으로 활용할 수 있을 것이라고 판단된다.

• 한국약용작물학회지
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• 제21권5호
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• pp.394-400
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• 2013

This study was carried out in order to examine the germination characteristics of Atractylodes japonica seeds, and it was to develop the more efficient pre-treatment and production system of the seeds. Experiment was performed by two ways-temperature control (10, 15, 20, $25^{\circ}C$) and shading treatment (full sunlight, 35, 50, 75, 95% of full sunlight). Seed pre-treatment before the each experiment was carried out by temperature (with low temperature and wetting treatment (LTW) for 0, 15, 30, 45 and 60 days) and shading treatment (with drying at room temperature (DRT), drying at low temperature (DLT) and water soaking (WS) for 48 hours). Seeds of A. japonica were germinated well under temperature control (overall 85.3~100%), especially high temperature. And, the seeds pre-treated with LTW for 45 days germinated with a 100% success rate. Under the surveying shading treatment, the highest germination rate was 95.8% with DRT and overall 63.2~95.8%. Germination rate under seeds with WS was 63.2~7.8%. As a result of surveying the whole experiment, A. japonica seeds don't need to pre-treat cause of high germination, but if it is performed with LTW for a certain period of time, it would be more productive.

• Plant Resources
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• 제3권3호
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• pp.179-183
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• 2000

The present study was carried out to clarify the chromosome numbers and karyotype of Atractylodes japonica Koidz. ex Kitam. and A. macrocephala Koidz.. The somatic chromosome numbers of two species were same; basic chromosome number x=12, and somatic chromosome numbers 2n=24. The present result of A. japonica Koidz. ex Kitam. was same to previously reports and that of A. macrocephala Koidz. was reported first in this study. Size and shape of chromosome were some different from A. japonica Koidz. ex Kitam. and A. macrocephala Koidz.. The karyotype of A. japonica Koidz. ex Kitam. was described as follows; 2n : 24 : 8L + 14M +2S : 2 $A^{sm}$ +2 $B^{m}$ +2 $C^{m}$ +2 $D^{st}$ + 2 $E^{m}$ +2 $F^{m}$ +2 $G^{m}$ +2 $H^{sm}$ + 2 $I^{m}$ + 2 $J^{m}$ + 2 $K^{m}$ + 2 $L^{m}$ . And the karyotype of A. macrocephata Koidz. was described as follows; 2n : 24 : 10L +12M +25 : 2 $A^{m}$ +2 $B^{sm}$ +2 $C^{sm}$ +2 $D^{sm}$ +2 $E^{sm}$ +2 $E^{sm}$ +2 $G^{sm}$ +2 $H^{m}$ +2 $I^{m}$ 2 $J^{m}$ +2 $K^{m}$ +2 $L^{m}$ . .

• 한국약용작물학회지
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• 제12권1호
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• pp.53-59
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• 2004

국내 한약재 시장에서 유통되고 있는 백출 건재약재의 기원을 판별하고자 해부형태적 특징과 종 특이적인 SCAR 마커 (SAjR2, SAmR1)를 적용한 실험 결과는 다음과 같다. 해부형태적 특징으로 A. japonica에서는 주피에 코르크층이 매우 발달하여 있었고, 코르크층 내부 또는 아래에 석세포대가 연속적으로 발달하고 있었다. 유실은 A. japonica에서 뿌리횡단면에 전체적으로 다수 분포하여 잘 발달하고 있었으며 피충부분과 잘 발달된 방사조직 내에서도 목섬유다발이 다수 관찰됐다. 또한 유세포 내의 침상 결정물도 A. macrocephala보다 많이 함유하고 있었다. 이러한 특징들을 종합한 결과, 국내 유통 국산 및 수입 백출은 모두 A. japonica의 해부형태적 특징을 따르고 있어 A. japonica로 동정되었다. 한편 RAPD clone으로부터 개발된 A. japonica 특이적인 SAjR2를 이용하여 PCR 한 결과 모든 샘플에서 600bp 크기의 한국백출 특이적인 밴드가 형성되었으며, A. macrocephala 특이적인 SAmR1을 이 용한 결과에서는 작물시험장에서 재배중인 A. macrocephala에서만 1200 bp크기의 특이적인 밴드가 형성되어, 시중에서 유통되고 있는 한국백출과 중국백출은 모두 A. japonica로 동정되었다. 결론적으로 해부형태적 특성과 SCAR 마커는 A. japonica와 A. macrocephala 기원식물의 동정과 유통 건재 약재를 판별하기에 유용한 방법이었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2021년도 춘계학술대회
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• pp.113-113
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• 2021