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Discrimination of Atractylodes Rhizome White Using Anatomical Characteristics and SCAR Markers  

Bang, Kyong-Hwan (Division of Ginseng & Medicinal Crop, National Institute of Crop Science, RDA)
Sung, Jung-Sook (Division of Ginseng & Medicinal Crop, National Institute of Crop Science, RDA)
Park, Chung-Heon (Division of Ginseng & Medicinal Crop, National Institute of Crop Science, RDA)
Jin, Dong-Chun (Division of Ginseng & Medicinal Crop, National Institute of Crop Science, RDA)
Park, Chun-Geon (Division of Ginseng & Medicinal Crop, National Institute of Crop Science, RDA)
Yu, Hong-Seob (Division of Ginseng & Medicinal Crop, National Institute of Crop Science, RDA)
Park, Hee-Woon (Division of Ginseng & Medicinal Crop, National Institute of Crop Science, RDA)
Seong, Nak-Sul (Division of Ginseng & Medicinal Crop, National Institute of Crop Science, RDA)
Publication Information
Korean Journal of Medicinal Crop Science / v.12, no.1, 2004 , pp. 53-59 More about this Journal
Abstract
Finding a means to discriminate the commercial herb medicines when they were dried and sliced is a very important and imminent project in Korea. To differentiate plant origins and the commercial herb medicines of Atractylodes japonica and A. macrocephala, two discriminative methods using anatomical characteristics and SCAR marker were applied. It was possible to discriminate plant origins and the commercial herb medicines between A. japonica and A. macrocephala by anatomical characteristics: development of periderm, layer of stone cell, distribution of laticiferous vessels, development of xylem fiber in xylem ray, contained quantity of clustered crystals and others. While, two SCAR markers were developed from RAPD clones: SAjR2 (600 bp) from AjR2 and SAmR1 (1,200 bp) from AmR1. These two markers were enough for discrimination plant origins and the commercial herb medicines between A. japonica and A. macrocephala. The result of application of anatomical characteristics and SCAR markers to investigate current status in domestic herb market, Daegu and Kumsan herb market, it was identified to be current herb medicines of A japonica.
Keywords
Atractylodes japonica; A. macrocephala; anatomical characteristics; SCAR marker; discrimination; commercial herb medicine;
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