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Evaluation of Late Blight Resistance and Agronomic Characteristics of Short-day Adapted Potato Germplasm (단일적응 감자 유전자원들의 역병저항성 및 주요 농업형질 평가)

  • Park, Young-Eun;Cho, Hyun-Mook;Cho, Ji-Hong;Cho, Kwang-Soo;Kim, Hyun-Jun;Landeo, Juan
    • Horticultural Science & Technology
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    • v.29 no.5
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    • pp.474-481
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    • 2011
  • Potato late blight caused by the fungus Phytophthora infestans is one of the most vital diseases damaging the potato plant. It is for this reason that breeding potato cultivars resistant to late blight is now becoming a major concern around the world. The B3C1 clones has been introduced by the Highland Agriculture Research Center, RDA. The clones which came from International Potato Center in 2005 have a durable resistance to late blight. The clones were bred under a short-day condition in Peru. However, there was still no report on the adaptability of these clones to the long-day condition in Korea. Therefore, this study was conducted to evaluate the late blight resistance and major agronomic characteristics of B3C1 clones under Korea's long-day condition. This study was also done to generate genetic resources for developing new varieties resistant to late blight. In this study it was found out that in naturally infested field with P. infestans, AUDPC (area under disease progress curve) values of all B3C1 clones were significantly lower than those of the control varieties, 'Superior', 'Atlantic', and 'Haryeong'. It was found out that B3C1 clones had a high level of resistance to late blight and that they could be used as genetic resources to breed potato varieties with late blight resistance. However, several undesirable characteristics such as extremely late maturity, excessive growth of stems and stolons, and production of tubers that cannot easily be removed from the stolons were also observed. Among the twenty B3C1 clones, two clones, LB-8 (CIP393077.159) and LB-11 (CIP393371.159), were selected for cultivating at the highland area of Korea. Two B3C1 clones were crossed with Korean breeding lines and clonal selection for the progenies is still in progress.

Dissolved Copper and Nickel in the surface water of East Sea, Korea (동해 표층수중 용존 Cu, Ni의 분포 특성)

  • Yoon, Sang Chol;Yoon, Yi Yong;Suh, Young Sang
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.17 no.4
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    • pp.257-267
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    • 2014
  • The distributions of trace metals in the East Sea were investigated during the R/V Lavrentyev cruise (July 2009) in which four transects from Russia shore to South were conducted to collect 25 surface water samples. The total dissolved concentrations of Cu and Ni were measured using ICP-MS, DRC-e. In the coastal area, their concentrations of Russia shore (Cu, 1.51; Ni, 1.82 nM) were 1.9 times for Cu and 2.0 times for Ni lower than Korea shore (Cu, 2.87; Ni, 3.71 nM). In the subregion, their concentrations of Warm region (Cu, 3.03; Ni, 2.28 nM) were higher for Cu than Cold region (Cu, 2.04; Ni, 2.28 nM). The distributions of Cu and Ni concentrations were divided by lowest level at $10^{\circ}C$ of water temperature. In this study period, the surface water temperatures of Russia shore and Japan basin were lower than $10^{\circ}C$ and them of Ulleung basin and Sakhalin shore were higher. Below $10^{\circ}C$, Cu and Ni concentrations increased when surface water temperatures decreased. Above $10^{\circ}C$, their concentrations increased with temperature, which showed highest concentrations in the Ulleung basin, directly influenced by flux from East Korean Warm Current. By comparing with other sea areas (Western Mediterranean, Atlantic), Cu concentrations in the East Sea were a little higher and Ni concentrations were lower. Particularly as the level of Cu in the offshore in the Ulleung basin were higher than in the coastal area, We can suggest that the atmospheric flux of Cu is relatively important in this area.

Spatial Distribution and Community Structure of Heterotrophic Protists in the Central Barents Sea of Arctic Ocean During Summer (북극해 하계 중앙 바렌츠해에서 종속영양 원생동물의 군집구조와 공간적 분포)

  • Yang, Eun-Jin;Choi, Joong-Ki;Kim, Sun-Young;Chung, Kyung-Ho;Shin, Hyoung-Chul;Kim, Yea-Dong
    • Ocean and Polar Research
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    • v.26 no.4
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    • pp.567-579
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    • 2004
  • To investigate the spatial distribution and community structure of heterotrophic protists, we collected water samples at 23 stations of central Barents Sea in August, 2003. This study area was divided into three area with physico-chemical and chi-a distribution characteristics: Area I of warm Atlantic water mass, Area III of cold Arctic water mass and Area II of mixed water mass. Chl-a concentration ranged from 0.18 to $1.04{\mu}g\;l^{-1}$ and was highest in Area I. The nano-sized chi-a accounted fur more than 80% of the total chi-a biomass in this study area. The contribution of nano-sized chi-a to total chi-a was higher in Area I than in Area II. Communities of heterotrophic protists were classified into three groups such as heterotrophic nanoflagellates (HNF), ciliates and heterotrophic dinoflagellates (HDF). During the study periods, carbon biomass of heterotrophic protists range from 11.3 to $38.7{\mu}gC\;l^{-1}$ (average $21.0{\mu}gC\;l^{-1}$), and were highest in Area I and were lowest in Area III. The biomass of ciliates ranged from 4.2 to $19.3{\mu}gC\;l^{-1}$ and contributed 31.5-66.9% (average 48.1%) to the biomass of heterotrophic protists. Ciliates to heterotrophic protists biomass accounted fur more than 50% in Area I. Heterotrophic dinoflagellates biomass ranged from 5.7 to $18.4{\mu}gC\;l^{-1}$ and contributed 27.1 to 56.3% (average 42.8%) of heterotrophic protists. Heterotrophic dinoflakellates to heterotrophic protists biomass accounted fur about 50% in Area III. Heterotrophic nanoflageltate biomass ranged from 0.5 to $3.4{\mu}gC\;l^{-1}$ and contributed 3.2 to 19.6% (average 9.2%) of heterotrophic protists. Heterotrophic nanoflagellates to heterotrophic protists biomass accounted fur more than 10% in Area III. These results indicate that the relative importance and structure of heterotrophic protists may vary according to water mass. Heterotrophic protists and phytoplankton biomass showed strong positive correlation in the study area The results suggest that heterotrophic protists are important consumers of phytoplankton, and protists might play a pivotal role in organic carbon cycling In the pelagic ecosystem of this study area during the study period.

Intercomparison of Satellite Data with Model Reanalyses on Lower- Stratospheric Temperature (하부 성층권 온도에 대한 위성자료와 모델 재분석들과의 비교)

  • Yoo, Jung-Moon;Kim, Jin-Nam
    • Journal of the Korean earth science society
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    • v.21 no.2
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    • pp.137-158
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    • 2000
  • The correlation and Empirical Orthogonal Function (EOF) analyses over the globe have been applied to intercompare lower-stratospheric (${\sim}$70hPa) temperature obtained from satellite data and two model reanalyses. The data is the19 years (1980-98) Microwave Sounding Unit (MSU) channel 4 (Ch4) brightness temperature, and the reanalyses are GCM (NCEP, 1980-97; GEOS, 1981-94) outputs. In MSU monthly climatological anomaly, the temperature substantially decreases by ${\sim}$21k in winter over southern polar regions, and its annual cycle over tropics is weak. In October the temperature and total ozone over the area south of Australia remarkably increase together. High correlations (r${\ge}$0.95) between MSU and reanalyses occur in most global areas, but they are lower (r${\sim}$O.75) over the 20-3ON latitudes, northern America and southern Andes mountains. The first mode of MSU and reanalyses for monthly-mean Ch4 temperature shows annual cycle, and the lower-stratospheric warming due to volcanic eruptions. The analyses near the Korean peninsula show that lower-stratospheric temperature, out of phase with that for troposphere, increases in winter and decreases in summer. In the first mode for anomaly over the tropical Pacific, MSU and reanalyses indicate lower-stratospheric warming due to volcanic eruptions. In the second mode MSU and GEOS present Quasi-Biennial Oscillation (QBO) while NCEP, El Ni${\tilde{n}}$o. Volcanic eruption and QBO have more impact on lower-stratospheric thermal state than El Ni${\tilde{n}}$o. The EOF over the tropical Atlantic is similar to that over the Pacific, except a negligible effect of El Ni${\tilde{n}}$o. This study suggests that intercomparison of satellite data with model reanalyses may estimate relative accuracy of both data.

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Phylogentic Position, Pigment Content and Optimal Growth Condition of the Unicellular Hydrogen-Producing Cyanobacterial Strains from Korean Coasts (한국 연안산 단세포성 수소생산 남세균 종주들의 분류계통, 색소함량 및 최적성장 환경)

  • PARK, JONG-WOO;KIM, JU HEE;CHO, AE-RA;JUNG, YUN-DUK;KIM, PYOUNG JOONG;KIM, HYUNG-SEOP;YIH, WONHO
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.20 no.3
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    • pp.131-140
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    • 2015
  • To set up unicellular cyanobacterial strains with photo-biological $H_2$ production potential, live samples were repeatedly collected from 68 stations in the coastal zone of Korea for the four years since 2005. Among 77 cyanobacterial strains established six (KNU strains, CB-MAL002, 026, 031, 054, 055 and 058) were finally chosen as the excellent strains for $H_2$ production with $H_2$ accumulation over 0.15 mL $H_2\;mL^{-1}$ under general basic $H_2$ production conditions as well as positive $H_2$ production for more than 60 hr. To explore optimum procedures for higher $H_2$ production efficiency of the six cyanobacterial strains, the inter-strain differences in the growth rate under the gradients of water temperature and salinity were investigated. The maximum daily growth rates of the six strains ranged from 1.78 to 2.08, and all of them exhibited $N_2-fixation$ ability. Based on the similarity of the 16S rRNA sequences, all the test strains were quite close to Cyanothece sp. ATCC51142 (99%). The six strains, however, were grouped into separate clades from strain ATCC51142 in the molecular phylogeny diagram. Chlorophyll- a content was 3.4~7.8% of the total dried weight, and the phycoerythrin and phycocyanin contents were half of those in the Atlantic strain, Synechococcus sp. Miami BG03511. The growth of the six strains was significantly suppressed at temperatures above the optimal range, $30{\sim}35^{\circ}C$, to be nearly stopped at $40^{\circ}C$. The growth was not inhibited by high salinities of 30 psu salinity in all the strains while strain CB055 maintained its high growth rate at low salinities down to 15 psu. The euryhaline strains like CB055 might support massive biotechnological cultivation systems using natural basal seawater in temperate latitudes. base seawater. The biological and ecophysiological characteristics of the test strains may contribute to designing the optimal procedures for photo-biological $H_2$ production by unicellular cyanobacteria.

Reproductive Performance of Holstein Dairy Cows Grazing in Dry-summer Subtropical Climatic Conditions: Effect of Heat Stress and Heat Shock on Meiotic Competence and In vitro Fertilization

  • Pavani, Krishna;Carvalhais, Isabel;Faheem, Marwa;Chaveiro, Antonio;Reis, Francisco Vieira;da Silva, Fernando Moreira
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.3
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    • pp.334-342
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    • 2015
  • The present study was designed to evaluate how environmental factors in a dry-summer subtropical climate in Terceira-Azores (situated in the North Atlantic Ocean: $38^{\circ}43^{\prime}N27^{\circ}12^{\prime}W$) can affect dairy cow (Holstein) fertility, as well as seasonal influence on in vitro oocytes maturation and embryos development. Impact of heat shock (HS) effects on in vitro oocyte's maturation and further embryo development after in vitro fertilization (IVF) was also evaluated. For such purpose the result of the first artificial insemination (AI) performed 60 to 90 days after calving of 6,300 cows were recorded for one year. In parallel, climatic data was obtained at different elevation points (n = 5) from 0 to 1,000 m and grazing points from 0 to 500 m, in Terceira island, and the temperature humidity index (THI) was calculated. For in vitro experiments, oocytes (n = 706) were collected weekly during all year, for meiotic maturation and IVF. Further, to evaluate HS effect, 891 oocytes were collected in the cold moths (December, January, February and March) and divided in three groups treated to HS for 24 h during in vitro maturation at: C (Control = $38.5^{\circ}C$), HS1 ($39.5^{\circ}C$) and HS2 ($40.5^{\circ}C$). Oocytes from each group were used for meiotic assessment and IVF. Cleavage, morula and blastocyst development were evaluated respectively on day 2, 6, and 9 after IVF. A negative correlation between cow's conception rate (CR) and THI in grazing points (-91.3%; p<0.001) was observed. Mean THI in warmer months (June, July, August and September) was $71.7{\pm}0.7$ and the CR ($40.2{\pm}1.5%$) while in cold months THI was $62.8{\pm}0.2$ and CR was $63.8{\pm}0.4%$. A similar impact was obtained with in vitro results in which nuclear maturation rate (NMR) ranged from 78.4% (${\pm}8.0$) to 44.3% (${\pm}8.1$), while embryos development ranged from 53.8% (${\pm}5.8$) to 36.3% (${\pm}3.3$) in cold and warmer months respectively. In vitro HS results showed a significant decline (p<0.05) on NMR of oocytes for every $1^{\circ}C$ rising temperature ($78.4{\pm}8.0$, $21.7{\pm}3.1$ and $8.9{\pm}2.2$, respectively for C, HS1, and HS2). Similar results were observed in cleavage rate and embryo development, showing a clear correlation (96.9 p<0.05) between NMR and embryo development with respect to temperatures. Results clearly demonstrated that, up to a THI of 70.6, a decrease in the CR occurs in first AI after calving; this impairment was confirmed with in vitro results.

Development of a Nutrient Solution for Potato (Solanum tuberosum L.) Seed Tuber Production in a Closed Hydroponic System (수경재배용 감자 배양액 개발)

  • Chang, Dong-Chil;Kim, Sung-Yeul;Shin, Kwan-Yong;Cho, Young-Ryul;Lee, Yong-Beom
    • Horticultural Science & Technology
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    • v.18 no.3
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    • pp.334-341
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    • 2000
  • To develop a nutrient solution for a closed hydroponic system in potato (Solanum tuberosum L.) 'Atlantic' and 'Superior' potatoes were grown with the nutrient solutions whose strengths were 0.25, 0.5, 1.0, and 1.5 of the concentration of the nutrient solution developed by the National Horticultural Experiment Station in Japan. The best results in potato growth and yield were obtained with 0.5 and 1.0 strength nutrient solutions, and nutrient compositions for potato were determined based on the 1.0 strength nutrient solution; $14.4me{\cdot}L^{-1}\;N,\;4.2me{\cdot}L^{-1}\;P,\;7.5me{\cdot}L^{-1}\;K,\;5.5me{\cdot}L^{-1}\;Ca$, and $3.5me{\cdot}L^{-1}\;Mg$ for stolon growth stage and $14.8me{\cdot}L^{-1}\;N,\;4.0me{\cdot}L^{-1}\;P,\;8.5me{\cdot}L^{-1}\;K,\;6.5me{\cdot}L^{-1}\;Ca$, and $3.0me{\cdot}L^{-1}\;Mg$ for tuber growth stage. To examine the suitability of the nutrient solutions developed for potato, the strengths of 1.0 (PS 1.0S), 0.75 (PS 0.75S), or 0.5 (PS 0.5S) were compared with half-strength of Japanese Horticultural Experiment Station' solution (JH 0.5S). Changes in pH, EC, and mineral concentrations in nutrient solutions depended more on solution strength and growth stage than on the type of nutrient solution. However, most elements in solution remained constant with plant age in PS 0.75S solution during stolon growth stage, and in PS 0.5S solution during tuber growth stage. The greatest growth and tuber yield was obtained in the standard strength solution (PS 1.0S), and potato solution developed in this experiment was recommended for hydroponic culture of potato in a closed system.

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A Study on the Role of United Nations Regional Group System for the London Protocol (런던의정서에서 유엔 지역그룹체제의 역할에 관한 연구)

  • Moon, Byung-Ho;Hong, Gi-Hoon
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.13 no.3
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    • pp.135-150
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    • 2010
  • At the Intergovernmental Meeting held in 1972, the London Convention was adopted to prevent marine pollution from dumping of wastes and other matter. After that, at the special meeting held at the Headquarters of the International Maritime Organization in 1996, the London Convention was revised to consider advances in technology of treatment and disposal of wastes and to reflect changes in understanding of marine environment and then the London Protocol was concluded. The London Protocol states more concrete management system for ocean dumping than the London Convention and also provides that the Meeting of Contracting Parties shall establish those procedures and mechanisms necessary to assess and promote compliance with the Protocol. With the London Protocol in force since 24 March 2006, the Meeting of Contracting Parties adopted the 'Compliance Procedures and Mechanisms (CPM) pursuant to Article 11 of the 1996 Protocol to the London Convention 1972' and established the Compliance Group in 2007. According to the CPM, members of the Compliance Group shall be nominated by Contracting Parties, based on equitable and balanced geographic representation of the five Regional Groups of the United Nations, and elected by the Meeting of Contracting Parties. In 2009, the Republic of Korea nominated a member of the Compliance Group to be subsequently elected by the Meeting of Contracting Parties with the approval of other states in Asia Group. Through the United Nations Regional Group System based on geographical identity or political affinity, Contracting Parties to the London Protocol are expected to form a voting bloc or to exchange information in meetings on the London Protocol. In this sense, it is noteworthy that the London Protocol introduced marine environmental management system for comprehensive prohibition of ocean dumping with exception of the so-called 'reverse-list' which had been earlier adopted by the 'Convention for the Protection of the Marine Environment of the North-East Atlantic, 1992 (OSPAR)' whose contracting parties belonged to Western European and Other States Group. In recent years, the jurisdiction of London Protocol has been extended to protect and preserve the marine environment from all sources of pollution. This will make the United Nations Regional Group System play more important roles in the activities associated with the London Protocol. For this reason, this article has considered characteristics of the United Nations Regional Group System and has analyzed influences of this Regional Group System in meetings on the London Protocol. This could provide preliminary information for the Republic of Korea to give due consideration to the United Nations Regional Group System on the activities associated with the London Protocol.

Investigation on Characteristics of Summertime Extreme Temperature Events Occurred in South Korea Using Self-Organizing Map (자기조직화지도(Self-Organizing Map)를 이용한 최근 우리나라 여름철 극한온도 특성 분류)

  • Lim, Won-Il;Seo, Kyong-Hwan
    • Atmosphere
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    • v.28 no.3
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    • pp.305-315
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    • 2018
  • This study investigates the characteristic spatial patterns and dynamic processes associated with the summertime extreme temperature events in South Korea during the last 20 years (1995~2014) using Self-Organizing Map (SOM). The classified SOM patterns commonly have high temperature and anticyclonic circulation anomalies over South Korea. The two major teleconnection patterns are identified: one is from the subtropical western North Pacific (WNP) affecting to the north and the other is from the North Atlantic (NA) affecting downstream region. The meridional teleconnection pattern is related to the forcing of positive sea surface temperature (SST) anomaly over the WNP. The northward propagating Rossby wave generates the East Asia-Pacific (EAP) pattern to form an anticyclonic circulation anomaly over South Korea. On the other hand, NA SST anomalies generate an eastward Rossby wave train across the Eurasian continent, leading to the development of an anticyclonic circulation anomaly over South Korea. The EAP pattern occurs more frequently in July and August, whereas the midlatitude teleconnection pattern associated with NA SST anomalies develops more frequently in early summer (June).

Evaluation of Potato Genetic Resources and Development of Potato Varieties with Diverse colors (감자 유전자원 평가 및 다양한 컬러 감자 품종 개발)

  • 임학태;이규화;구동만;양덕춘;전익조
    • Korean Journal of Plant Resources
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    • v.16 no.3
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    • pp.264-274
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    • 2003
  • Many potato genetic resources have been collected and improved for their diverse traits over the years using breeding program in KPGR. To select potential varieties for table and processing in Korea, 58 elite potato breeding lines and several 'Valley' varieties were cultivated and harvested at Korea Alpine area in 2001. The cultivated lines and varieties were evaluated using their cultural adaptability in the environment and tuber characteristics, such as the depth of tuber eye, tuber shape, skin color, flesh color, scab resistance, yield, and the resistance of hollow heart and internal brown spot disease. Additionally, in the selection of potential processing varieties, reducing sugar content (sum of glucose and fructose concentration) of tubers is critically considered, because it mainly influence on the chip color of processing potato tuber. For table stock varieties with white skin color, 'Early Valley', 'Summer Valley', 'Winter Valley', and 'Taebok Valley' were selected. In the aspect of diverse potato tuber color, several varieties were selected such as 'Golden Valley' for its yellow fresh and skin color, 'Gogu Valley', 'Juice Valley', and 'Rose Valley' for their red skin color, and 'Purple Valley' for its purple skin. Compared with world wide known processing cultivar 'Atlantic', 24 lines (or varieties) were selected for the potential potato processing industry due to their low reducing sugar contents (below 0.3%), high yield (above 4.0 ton/ha), and unique chip colors. Selected white chipping varieties were 'Taedong Valley', 'Kangshim Valley', and 'Kangwon Valley', which have 0.23%, 0.27%, and 0.29% of reducing sugar contents, respectively. 'Bora Valley', having deep purple color in both skin and fresh, was selected for purple chip variety and has 0.26% of reducing sugar content. Light yellow chip varieties (lines) were 'Rose Valley' and Valley 54, having 0.19% and 0.269% of reducing sugar content, respectively. For French frying potatoes, 'Stick Valley' of 0.22% and Valley 72 of 0.151% in reducing sugars were selected. All of these selected lines and 'Valley' varieties can be used as parents to improve potato genetic resources and to develop better varieties with unique traits and colors.