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Application of Slow-Freezing Cryopreservation Method for the Conservation of Diverse Potato (Solanum tuberosum L.) Genotypes

  • Zhao Mei-Ai;Dhital Shambhu P.;Fang Yi-Lan;Khu Dong-Man;Song Ye-Su;Park Eung-Jun;Kang Chang-Won;Lim Hak-Tae
    • Journal of Plant Biotechnology
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    • v.7 no.3
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    • pp.183-186
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    • 2005
  • Cryopreservation has been recognized as a practical and efficient tool for the long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of slow-freezing techniques on the cryopreservation of potato. In vitro plantlets of the potato genotypes of 'Atlantic', 'Superior’, 'Namseo', 'J138', and 'CTO5-5' were cold acclimated, and the excised axillary buds were precultured, osmoprotected, exposed to plant vitrification solution, frozen slowly to $-40^{\circ}C$ and then rapidly plunged into liquid nitrogen, thawed and finally plated on the regeneration medium. It was found that the higher the sucrose concentrations in the subculture medium of donor plantlets, the higher the survival rates of shoot tips after cryopreservation, and the highest survival (20%) was observed in the medium added with 0.25 M sucrose. As for the effect of cooling, $0.3^{\circ}C/min$ cooling speed showed the highest survival (25%). Different varieties showed different responses over different cryopreservation treatments. Survival rate was increased by slow-freezing technique method as compared with that of the basic cryopreservation method of vitrification alone in the diverse potato genotypes. Leaf and tuber morphologies of potatoes regenerated after cryopreservation using slow freezing technique were similar to those derived from the in vitro stock plantlets.

A Study of the Synoptic Climatology on the January's Cold and Warm Winter Especially in 600hPa Circulation : Case Study 1992 and 1984 in January (500hPa면 순환특성을 중심으로 한 동계 이상 한.난월의 종관기후학적 연구 -1992년과 1984년의 1월의 경우-)

  • Lee, Byung-Gon;Min, Woo-Ki
    • Journal of the Korean association of regional geographers
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    • v.2 no.2
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    • pp.103-111
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    • 1996
  • I followed the results of Lee and Min(1996) for classification of the months of cold and warm winter. The winter of 1992 and 1984 recorded extraordinary cold and warm. Study of the Synoptic Climatology on the January's cold and warm winter is below: (1) Climatology's characteristic. Temperature of extremely high temperature month is higher compared with extremely low temperature month. Also precipitation is more than over low temperature month compared with extremely high temperature month. (2) In circulation of 500hPa surface. (1) Extremely high temperature month At 500hPa, negative geopotential height anomalies in high latitude, three trough developed over eastern Canada. In midlatitude, a deep trough persisted in the central North Pacific and conspicuous positive height anomalies showed over northwestern Europe, Where a blocking anticyclone developed. It had been warmer than normal since last year in Korea due mainly to positive height anomalies stretched from central Siberia (2) Extremely low temperature month Appeared the strong meridional circulation and negative height anomalies showed from Far East to the Mid-Pacific and appeared ridge in the west of the North America and Atlantic. Alutien Low shows negative deviation during 1984. In northern hemisphere shows negative deviation. Therefore, we can show that the surface pressure distribution and height distribution of 500hPa level are closely connected with each other as parts of general circulation. (3) The characteristics of the general circulation pattern of the 500hPa (1) Extremely high temperature month is high than extremely low temperature month1984 in Zonal index (2) The majority type is S type in 500hPa level circulation of extremely high temperature month but extremely high temperature month is M type (3) The wave number in 500hPa all shows 3 wave. So can not distinguished by only predominant wave number pattern.

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xDSL 표준화동향

  • 김기호
    • Information and Communications Magazine
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    • v.14 no.12
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    • pp.77-85
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    • 1997
  • 지금까지 access network에서의 xDSL의 위치를 살펴보고 DSL, HDSL, ADSL 및 VDSL을 중심으로 기술 및 최근의 표준화동향을 살펴보았다. ITU의 조사에 따르면 97년 기준으로 전세계 약 8억가구에 copper access line이 연결되어 있고 그중의 70%이상이 가정에 연결되어 있는데 증가추세도 북미의 경우 년3.5%, 서유럽과 태평양연안의 경우 년4.5%의 성장율을 보이고 있어 2000년경에는 10억가구에 다다를 전망이다. 미국은 97년기준 약 1억가구에 1억6000만 copper access line이 연결되어 있는데 Yankee Group이 조사한 바에 따르면 2000년을 기준으로 3백만가구에 ADSL/RADSL/SDSL이 연결되고 관련 revenue는 10억달러에 이를것으로 추산하고 있다. 전세계 xDSL 시장규모를 미국의 5배정도로 보면 2000년경에는 전세계 twist pair의 2%정도에 xDSL이 연결되어 ADSL/RADSL/SDSL 관련 세계시장규모가 50억달러에 이르고 시장성장률은 30%에 이를 것으로 보고 있다. 현재 이러한 xDSL의 표준화작업은 3장에서 언급한 바와 같이 ANSI T1, ETSI에서 주로 주도되고 있으나 ITU-T, IEEE, ADSL Forum, ATM Forum, DAVIC 등 다른 group과 활발한 liasion meeting 을 통해서도 협력안을 마련하려 노력하고 있다. 예를 들어 DAVIC은 long range(1.5Km이상) PMD규격으로 ADSL을, mid range($300m\sim1.5km$) PMD규격으로 VDSL을, short range(300m이하) PMD규격으로 CAP방식의 FTTC를 각각구정하여 xDSL을 수용하고 있다. Microsoft의 CEO인 Bill Gates가 'Bandwidth bottleneck. No question. that's the biggest obstacle.'이라고 천명한 바와 같이 정보사회로의 진전에 있어 bandwidth는 시급히 해결되어야 할 문제이다. 정보사회의 꿈을 실현하고자 1993년부터 시작된 In-formation Infrastructure의 구축노력이 그동안 다양하게 시도되어왔으나 숱한 우여곡절과 실패를 겪은 telco등의 서비스업자들이 이제는 너무 큰 꿈을 쫓기보다는 internet이라는 현실적인 시장에서 xDSL과 cablemodem을 바탕으로 차근차근 새로 시작하고 있다고 생각된다. 그동안 ADSL, Forum을 중심으로한 일부 technology evangelist들은 전세계 8억가구에 깔려있는 copper twist pair를 경제적인 'the last mile solution'으로 활용할 것을 줄기차게 주장해왔다. 그동안 xDSL은 VDSL이라는 차세대 대안을 제시하여 interim solution이라는 울타리를 벗어나는데 성공하였을 뿐만 아니라 IDSL, SDSL, RADSL등의 새로운 고객지향적 xDSL을 개발하여 선택의 폭을 넓혀주었다. xDSL vendor들간의 치열한 경쟁속에서 값싼 xDSL 시스템들이 속속 등장하고 있으며, DSLAM[8]과 같은 switch solution도 제시되어 기존의 copper twist pair를 이용해 값싸게 다양한서비스를 제공해 줄 수 있는 기틀을 마련한 것이 오늘날의 xDSL의 성공요인이라고 생각된다. 일찌기 Bell Atlantic의 CEO Ray Smith가 'Yes. ADSL is an interim solution that will be with us for forty years.'라고 간파한대로 xDSL은 fiber가 좀 더 값싼 해결책이 될 때까지 당분간 access network에서 확고한 자리를 구축할 것으로 보인다. 최근들어 우리나라의 초고속정보통신망도 ADSL 과 VDSL을 바탕으로 FTTH으로 진화하는 전략을 수립한것은 우리의 현실과 세계적인 추세를 반영한 일이라 여겨진다.

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On an Apparatus of Visualization for Magnetic Reversal and Magnetic Stripes (자기역전 시각화 장치와 지자기띠에 대하여)

  • Ryoo, Chung-Ryul
    • The Journal of the Petrological Society of Korea
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    • v.25 no.1
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    • pp.85-88
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    • 2016
  • The new rocks of the oceanic crust, like basalt, are created in the mid-oceanic ridge, and the magnetic polarities of the rocks are supposed to be oriented as following the Earth's magnetic field. An extensive magnetic survey of total field at sea level reveals mainly unusual north-south magnetic stripes parallel to the axis of the mid-oceanic ridge, especially in the Atlantic Ocean. From this stripes the Earth's magnetic field is considered as repeatedly 'flipped'(the N pole becoming the S pole, and vice versa) and many times over geological time. The discovery of stripes of alternately normal and reversed-magnetized rocks forming the ocean floor has been a key evidence for the sea-floor spreading, continental drift, and plate tectonics. This study introduces a simple apparatus to explain a possible mechanism of the magnetic reversal in the new oceanic crust, which makes a magnetic stripe adjacent to the mid-oceanic ridge. The apparatus shows a bar magnet effect of adjoined stripes to have a special magnetic polarity on the rocks in the center of the mid-oceanic ridge. The new magnetic stripe seems to be generated not only by Earth's magnetic field, but also by neighbored stripes in the mid-oceanic ridge, acting as a bar magnet.

An Approach to Regionalism on the Creation of a European Single Aviation Market: From the Viewpoint of the EU's Strategic Response against the US (유럽 단일항공시장 형성에 관한 지역주의적 접근 - EU의 미국에 대한 전략적 대응 관점에서 -)

  • Suh Jeongwook
    • Journal of the Korean Geographical Society
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    • v.39 no.6 s.105
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    • pp.955-970
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    • 2004
  • With the flow of globalization, world aviation markets are showing growing trends toward liberalization, deregulation, and privatization. At the same time, also apparent is a strong trend toward regionalism on aviation markets to realize open skies at the regional level. This research examines the recent trend of regionalism in the world aviation market from a political economic perspective, taking a single European aviation market as an example. The results show that the creation of a single European aviation market is the EU's strategic response against the US to enhance the European aviation industry's competitiveness. More specifically, the results show that the European countries are creating a single European aviation market in order to stand against the US' so-called 'divide and nile policy' and 'encirclement strategy' and to make their airliners to have competitive advantages over the US airliners on the lucrative North-Atlantic air routes. Recently, the single European aviation market is expanding by including the Mediterranean and Balkan states to pursue Pan European aviation cooperation and to develop European Common Aviation Area so that it can continuously enhance its negotiating leverage against external markets. The results imply that it is urgent for the Northeast Asian countries to create their own regional aviation bloc to develop their aviation industry and to increase their negotiating power against external countries.

Occurrence Pattern of Insect Pests on Several Varieties of Potato (감자 품종별 해충 발생 양상)

  • Kwon, Min;Park, Chun-Soo;Hahm, Young-Il
    • Korean journal of applied entomology
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    • v.36 no.2
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    • pp.145-149
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    • 1997
  • Occurrence pattern of major insect pests on 7 recommended potato cultivars in Korea was investigated from sowing to harvesting time at Kangneung and Daegwallyung experiment fields in 1996. Green peach aphid (Mym persicae Sulzer), potato aphid (Macrosiphum euphorbiae Thomas) and beet armyworm (Spodoptera enigua Hubner) were the major insect pests on potato leaves, and wireworm (Selatosomus puncticollis Mot.) was the major pest on tubers. There was a slight difference of average number of aphids per 50 leaves among cultivars; ranged from 22.7 on cv. Dejima to 46.3 on cv. Superior. Numbers of leaves damaged by beet armyworm larvae on cv. Shepody and cv. Jopung were 11.0 and 14.3, and these cultivars are thought to be resistant against the larvae infestation. However, degree of damage on cv. Dejima and cv. Namsuh was higher 10 times than cv. Shepody and cv. Jopung. In wireworm, the percentage of damaged tubers on cv. Irish Cobbler was lowest of 8.1%, and followed by cv. Superior, cv. Dejima, cv. Jopung, and cv. Atlantic. Whereas, those on cv. Namsuh and cv. Shepody were significantly high of 50.0% and 46.8%.

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Analysis of Tropospheric Zenith Path Delay of GPS Code Based Precise Time Comparison Technique (GPS 코드 기반 정밀시각비교기법의 대류층 천정지연모델 분석)

  • Yu, Dong-Hui;Yang, Sung-Hoon;Do, Jae-Chul;Lee, Chang-Bok
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.12
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    • pp.61-69
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    • 2012
  • This paper shows results of the precise time comparison technique based on GPS code transfer in order to determine the UTC(Universal Time Coordinated) and generate TAI(International Atomic Time). CGGTTS(CCTF Group on GNSS Time Transfer Standards) which is generated by GPS timing receivers is used as the international standard format. For geodetic receivers which provide RINEX formats as GPS time transfer results, ROB(Royal Observatory of Belgium) developed a conversion program, r2cggtts, and have distributed the program to timing laboratories participating in TAI link all over the world. Timing laboratories generate the time comparison results of GPS code transfer by the program and send them to BIPM(Bureau International des Poids et Mesures) periodically. In this paper, we introduce the delay features generated while GPS code is transferred and the calibration methods of them. Then, we introduce the tropospheric delay and analyze the results of Saastamoinen model and NATO(North Atlantic Treaty organization) model. Saastamoinen model is the representative tropospheric zenith path delay model and NATO model is applied to the legacy r2cggtts program.

Group-Based Frequency Hopping Scheme for Improving Multi-Net Performance of Link-16 Waveform with Limited Frequency Band (제한된 주파수 대역에서 Link-16 웨이브폼의 멀티넷 성능 향상을 위한 그룹 기반의 주파수 도약 방식)

  • Yu, Jepung;Lee, Kyuman;Baek, Hoki;Lim, Jaesung;Kim, Jongsung;Choi, Hyogi
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.41 no.1
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    • pp.110-121
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    • 2016
  • Link-16 is a representative TDL operated by US air force and NATO and supports structure of Multi-net. Under Multi-net, military operation can be conducted effectively since terminal nodes in Link-16 hop over total frequency band simultaneously. As air traffic is rapidly increasing, new aeronautical system is introduced or existing system should be expanded to accommodate increasing air traffic and frequency band assigned for operating this system is scarce. It is scheduled to implement frequency remapping to solve frequency scarcity. With limited frequency band for operating Link-16, as frequency remapping is implemented, degradation of Multi-net performance can happen since multiple access interference in Link-16 is increasing so it is difficult to conduct multiple military operations. Thus, Group-based frequency hopping scheme is proposed to solve this problem. We verified the performance of the proposed scheme is improved.

Discrimination of Potato Varieties by Random Amplified Polymorphic DNA Analysis (RAPD에 의한 감자 품종의 구분)

  • Seo, Hyo Won;Yi, Jung Yoon;Cho, Hyun Mook;Park, Young Eun;Oh, Seung Eun
    • Horticultural Science & Technology
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    • v.19 no.1
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    • pp.29-33
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    • 2001
  • This study was carried out to discriminate potato cultivars and breeding lines by specific molecular markers using random amplified polymorphic DNA (RAPD) analysis. The genotypes of potatoes used for analysis were eight cultivars and five breeding lines. Some of those show much phenotypic resemblances among them because 'Jopung', 'Daekwan70', 'Gawon', and 'Daekwan72' have immediate parental relationship with 'Superior', 'Irish Cobbler', 'Namsuh', and 'Atlantic', respectively. So, there are many difficulties to distinguish the varieties by the morphological characteristics. Three URP primers, URP2, URP4, and URP8 were selected for promising primers to discriminate potato genotypes or cultivars. The three URP primers were shown very high reproducibility because of the relatively high annealing temperature and long primer size. Although the results of similarity analyses did not always reflect the genetic relationship between potato varieties, the reproducible pattern of amplified DNA bands by URP primers showed possibility for molecular markers for discrimination of potato genotype or cultivar.

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Handling of Data Base on the Catch of Bigeye Tuna Thunnus Obesus ( LOWE ) (눈다랭이 어획량의 데이터 베이스 처리)

  • Lee, Ju-Hee;Lee, Chun-Woo;Kim, Ju-Chean
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.27 no.4
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    • pp.225-231
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    • 1991
  • In order to suggest the useful information on the fishing ground of the bigeye tuna Thunnus obesus(LOWE), a data base system was formed with catch data of the Korean tuna long liners during from 1975 to 1987 by using a set of 16 bits personal computer. This data base was constructed of the handling program and 4 types of data file processed from the monthly and yearly catch data of the whole tunas and the bigeye tuna. And when the system was started, the map of one among various Oceans such as the Pacific, the Atlantic and the Indian Ocean. is drawn on the monitor. And then the catch rates of the whole tunas or the catch ratios of bigeye tunas are indicated by the figured symbols and the colors on the sea divisions of 5$^{\circ}$ space of longitude and latitude respectively at the same time. Also this system has the preestimating program on the catch rates of the whole tunas and the bigeye tuna in the desired month and sea divisions. In the results than this data base system was handled and tested, very useful informations were obtained for the detection of tunas, especially bigeye tuna, and the preestimation was possible in a desired level.

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