• Title/Summary/Keyword: Asymmetric addition

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Primer RNA Synthesis by E. coli RNA Polymerase on the SSB-coated 229-nt ssi Signal of Lactococcal Plasmid pGKV21 (Lactococcal plasmid pGKV21의 SSB-coated 229-nt ssi signal 상에서 E. coli RNA polymerase에 의한 시발체 RNA 합성)

  • Jeong, Jin-Yong;Kim, Eun-Sil;Kim, Sam-Woong;Kang, Ho-Young;Bahk, Jeong-Dong
    • Journal of Life Science
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    • v.19 no.3
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    • pp.305-310
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    • 2009
  • Plasmid pGKV21 contains a 229-nucleotide (nt) single-strand DNA initiation (ssi) signal. Using asymmetric PCR, we prepared a small single-stranded (ss) DNA fragment of the ssi signal and, using the 229-nt ssDNA fragment, determined the requirements of RNA polymerase for priming and DNA-protein interaction. The ssi fragment prepared was able to generate primer RNAs with almost the same efficiency as the $M13{\Delta}lac182/229$ phage DNA. However, the cssi (complementary strand of the ssi signal) fragment could not synthesize primer RNAs. This result suggests that the 229-nt ssi signal functions in a strand specific manner. Gel retardation and DNase I footprinting demonstrated that the synthesized ssi fragment could interact with both E. coli RNA polymerase and SSB protein to synthesize primer RNA. In Escherichia coli [pWVAp], an addition of rifampicin resulted in an accumulation of ssDNA, indicating that the host-encoded RNA polymerase is involved in the conversion of ssDNA to double-stranded plasmid DNA.

A Study on the Impact of Oil Price Volatility on Korean Macro Economic Activities : An EGARCH and VECM Approach (국제유가의 변동성이 한국 거시경제에 미치는 영향 분석 : EGARCH 및 VECM 모형의 응용)

  • Kim, Sang-Su
    • Journal of Distribution Science
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    • v.11 no.10
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    • pp.73-79
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    • 2013
  • Purpose - This study examines the impact of oil price volatility on economic activities in Korea. The new millennium has seen a deregulation in the crude oil market, which invited immense capital inflow into Korea. It has also raised oil price levels and volatility. Drawing on the recent theoretical literature that emphasizes the role of volatility, this paper attends to the asymmetric changes in economic growth in response to the oil price movement. This study further examines several key macroeconomic variables, such as interest rate, production, and inflation. We come to the conclusion that oil price volatility can, in some part, explain the structural changes. Research design, data, and methodology - We use two methodological frameworks in this study. First, in regards to the oil price uncertainty, we use an Exponential-GARCH (Exponential Generalized Autoregressive Conditional Heteroskedasticity: EGARCH) model estimate to elucidate the asymmetric effect of oil price shock on the conditional oil price volatility. Second, along with the estimation of the conditional volatility by the EGARCH model, we use the estimates in a VECM (Vector Error Correction Model). The study thus examines the dynamic impacts of oil price volatility on industrial production, price levels, and monetary policy responses. We also approximate the monetary policy function by the yield of monetary stabilization bond. The data collected for the study ranges from 1990: M1 to 2013: M7. In the VECM analysis section, the time span is split into two sub-periods; one from 1990 to 1999, and another from 2000 to 2013, due to the U.S. CFTC (Commodity Futures Trading Commission) deregulation on the crude oil futures that became effective in 2000. This paper intends to probe the relationship between oil price uncertainty and macroeconomic variables since the structural change in the oil market became effective. Results and Conclusions - The dynamic impulse response functions obtained from the VECM show a prolonged dampening effect of oil price volatility shock on the industrial production across all sub-periods. We also find that inflation measured by CPI rises by one standard deviation shock in response to oil price uncertainty, and lasts for the ensuing period. In addition, the impulse response functions allude that South Korea practices an expansionary monetary policy in response to oil price shocks, which stems from oil price uncertainty. Moreover, a comparison of the results of the dynamic impulse response functions from the two sub-periods suggests that the dynamic relationships have strengthened since 2000. Specifically, the results are most drastic in terms of industrial production; the impact of oil price volatility shocks has more than doubled from the year 2000 onwards. These results again indicate that the relationships between crude oil price uncertainty and Korean macroeconomic activities have been strengthened since the year2000, which resulted in a structural change in the crude oil market due to the deregulation of the crude oil futures.

Temperature Effect on the Optical Properties of YAG and Silicate Phosphor-based White Light Emitting Diodes (온도 변화에 따른 YAG 및 Silicate형광체 기반 백색 LED의 광특성 변화에 대한 연구)

  • Choi, Hyun-Woo;Ko, Jae-Hyeon
    • Korean Journal of Optics and Photonics
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    • v.24 no.3
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    • pp.135-142
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    • 2013
  • Two white light emitting diodes(LEDs) were fabricated by using two kinds of yellow phosphor, YAG:Ce and $(Sr,Ba)_2SiO4:Eu$, and their spectroscopic properties were analyzed as a function of temperature from room temperature to $80^{\circ}C$. The asymmetric double sigmoidal function was applied to both blue and yellow peaks of the emitting spectrum to obtain the center wavelength, the amplitude, the half width, and the skewness parameters. According to this analysis, the center wavelength of the blue peak shifted to longer wavelength while that of the yellow peak shifted to shorter wavelength. In addition, some of the skewness parameters were found to increase upon heating, which indicates that spectrum asymmetry becomes enhanced at higher temperatures. The changes in the color coordinates and the luminous efficacy were larger for the case of silicate-based white LED. These results suggest that the silicate-based white LED is inferior to the YAG-based white LED from the viewpoint of color stability, efficacy and color rendering index.

Design of MMIC SPDT Switches in the ISM Band Using GaAs MESFETs (GaAs MESFET를 이용한 ISM 대역 MMIC SPDT 스위치 설계)

  • Park, Hun;Yun, Kyung-Sik;Ji, Hong-Koo;Kim, Hae-Cheon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.28 no.3A
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    • pp.179-184
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    • 2003
  • In this paper, an asymmetric topology of MMIC SPDT switch was proposed to increase the isolation in the receiving path and decrease the insertion loss with higher P1dB in the transmitting path for the ISM band. This SPDT switch was implemented with 0.5㎛ GaAs MESFETs processed by ETRI for the IDEC MPW project. For the receiving path the measured insertion losses were 1.518dB at 3GHz and 1.777dB at 5.75GHz and the isolations were 38.474dB at 3GHz and 29.125dB at 5.75GHz. For the transmitting path the insertion losses were 0.916dB at 3GHz and 1.162dB at 5.75GHz and the isolations were 23.259dB at 3GHz and 16.632dB at 5.75GHz. Compared to the symmetric topology the isolations of the receiving path for the asymmetric one were improved by 15.9dB at 3GHz and 11.9dB at 5.75GHz and its insertion loss was increased by about 0.6dB. In addition, P1dB of 21.5 dBm for the transmitting path was obtained, which is increased by 3.86dB compared to the symmetric one.

Accelerating Effects of Ultrasonic Irradiation on Reaction Rates for the Asymmetric Ring Opening Reaction of Epoxides (초음파 조사에 의한 에폭사이드 비대칭 고리열림 반응의 속도 증진 효과)

  • Lee, Yae Won;Park, Geun Woo;Kim, Geon Joong
    • Applied Chemistry for Engineering
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    • v.30 no.3
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    • pp.365-370
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    • 2019
  • In this study, effects of the ultrasonic irradiation during the reaction process were investigated for the enantioselective kinetic resolution (EKR) reaction of racemic epoxides in the presence of chiral cobalt salen catalysts, as compared to that of using the conventional mechanical stirring. In order to compare catalytic activities, the chiral cobalt salen complexes having $AlCl_3-$, $BF_3-$ and nitrobenzenesulfonic acid (NBSA) were synthesized and used as catalysts, and then three kinds of the racemic epoxides such as ephichlorohydrine (ECH), epoxy phenoxypropane (EPP) and propylene oxide (PO) were used as reactants. In addition, EKR reactions have been performed using the water and methanol as nucleophiles, respectively. The unique contribution of ultrasonic irradiation as a powerful mixing medium was evaluated in this study to improve the kinetics in comparison to the conventional mechanical agitation during EKR reactions. The reaction time to obtain the highest 99 ee% became shorten more than that of above 60%, when the ultrasonic irradiation was used. This result may be interpreted by the cavitation effect of ultrasound in the solution, generating a powerful shear force for the very violent mixing.

Study Visual Characteristics of World Cup Emblems (월드컵 엠블럼에 나타난 시각적 특징 연구)

  • YongFeng Liu
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.3
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    • pp.519-528
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    • 2023
  • The visual characteristics of the World Cup emblem play an important role in conveying culture and identity, highlighting the key elements of the Games, and strengthening the brand image. This study first investigated the visual elements and theoretical background of the emblem through previous studies. Second, the selection of symbolism, shape, and color was the subject of the study. Third, based on the shape-related theory based on the '100 National Cultural Symbols', the analysis was performed using the 'IRI Color Matching Image Scale' as a tool, and a final conclusion was drawn. The World Cup emblem uses a lot of ethnic and living symbols, and the living and ethnic symbols are expressed in a combination. In the beginning, there were many symmetric shapes, but after that it changed to an asymmetric shape. The curved expression form is more used than the straight expression form. And most of the color arrangement image scale distribution shows light, dynamic and modern color arrangement characteristics. In the future, the World Cup emblem design will continue to use national and living symbols. The shape of the emblem should use curves and asymmetric designs, be intuitive, and directly reflect the theme of the competition. In addition, the national flag color should be used as the main color, and other colors should be used as secondary colors. The combination of colors must match the light, dynamic and modern color characteristics of the IRI color scheme image scale.

Preparation of Polyethersulfone Ultrafiltration Membranes Containing $ZrO_2$ Nanoparticles by Combining Phase-inversion Method/Sol-gel Technique (상변환/졸-겔법에 의한 $ZrO_2$ 나노입자 함유 Polyethersulfone 한외여과 막의 제조)

  • Youm, Kyung-Ho;Lee, Yun-Jae
    • Membrane Journal
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    • v.16 no.4
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    • pp.303-312
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    • 2006
  • The asymmetric hybrid membranes of polyethersulfone (PES) and $ZrO_2$ nanoparticles were prepared via new one-step procedure combining simultaneously the phase-inversion method and the sol-gel technique. The optimum contents of $Zr(PrO)_4\;and\;HNO_3$ catalyst were determined by the adsorption experiments of phosphate anion onto the resulting hybrid membranes. The maximum adsorption of phosphate anion is obtained at the conditions of 0.15 mL $Zr(PrO)_4$ addition per 1 mL PES and 30 mL $HNO_3$ addition per 1 mL $Zr(PrO)_4$. Variation of morphology, performance and incorporated $ZrO_2$ amount of the resulting hybrid membranes were discussed and determined using SEM, pure water flux, TGA, ICP, XRD and contact angle measurements. Increasing $Zr(PrO)_4$ addition into casting solution, pure water flux is increased and $ZrO_2$ amount in the hybrid membrane is maximized at the conditions 0.15 mL $Zr(PrO)_4$ addition per 1 mL PES. The prephosphatation of PES-$ZrO_2$ hybrid membrane was studied to modify the surface characteristics of membrane. Ultrafiltration of bovine serum albumin (BSA) solution was performed in a dead-end cell using both a bare (non-phosphated) and a phosphated hybrid membrane. It is revealed that both the permeate flux and BSA rejection were increased as about 40% by prephosphatation of hybrid membrane. These results may be explained on the basis of the increase of membrane hydrophilicity, which was determined from contact angle measurements.

An Intelligent Intrusion Detection Model Based on Support Vector Machines and the Classification Threshold Optimization for Considering the Asymmetric Error Cost (비대칭 오류비용을 고려한 분류기준값 최적화와 SVM에 기반한 지능형 침입탐지모형)

  • Lee, Hyeon-Uk;Ahn, Hyun-Chul
    • Journal of Intelligence and Information Systems
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    • v.17 no.4
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    • pp.157-173
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    • 2011
  • As the Internet use explodes recently, the malicious attacks and hacking for a system connected to network occur frequently. This means the fatal damage can be caused by these intrusions in the government agency, public office, and company operating various systems. For such reasons, there are growing interests and demand about the intrusion detection systems (IDS)-the security systems for detecting, identifying and responding to unauthorized or abnormal activities appropriately. The intrusion detection models that have been applied in conventional IDS are generally designed by modeling the experts' implicit knowledge on the network intrusions or the hackers' abnormal behaviors. These kinds of intrusion detection models perform well under the normal situations. However, they show poor performance when they meet a new or unknown pattern of the network attacks. For this reason, several recent studies try to adopt various artificial intelligence techniques, which can proactively respond to the unknown threats. Especially, artificial neural networks (ANNs) have popularly been applied in the prior studies because of its superior prediction accuracy. However, ANNs have some intrinsic limitations such as the risk of overfitting, the requirement of the large sample size, and the lack of understanding the prediction process (i.e. black box theory). As a result, the most recent studies on IDS have started to adopt support vector machine (SVM), the classification technique that is more stable and powerful compared to ANNs. SVM is known as a relatively high predictive power and generalization capability. Under this background, this study proposes a novel intelligent intrusion detection model that uses SVM as the classification model in order to improve the predictive ability of IDS. Also, our model is designed to consider the asymmetric error cost by optimizing the classification threshold. Generally, there are two common forms of errors in intrusion detection. The first error type is the False-Positive Error (FPE). In the case of FPE, the wrong judgment on it may result in the unnecessary fixation. The second error type is the False-Negative Error (FNE) that mainly misjudges the malware of the program as normal. Compared to FPE, FNE is more fatal. Thus, when considering total cost of misclassification in IDS, it is more reasonable to assign heavier weights on FNE rather than FPE. Therefore, we designed our proposed intrusion detection model to optimize the classification threshold in order to minimize the total misclassification cost. In this case, conventional SVM cannot be applied because it is designed to generate discrete output (i.e. a class). To resolve this problem, we used the revised SVM technique proposed by Platt(2000), which is able to generate the probability estimate. To validate the practical applicability of our model, we applied it to the real-world dataset for network intrusion detection. The experimental dataset was collected from the IDS sensor of an official institution in Korea from January to June 2010. We collected 15,000 log data in total, and selected 1,000 samples from them by using random sampling method. In addition, the SVM model was compared with the logistic regression (LOGIT), decision trees (DT), and ANN to confirm the superiority of the proposed model. LOGIT and DT was experimented using PASW Statistics v18.0, and ANN was experimented using Neuroshell 4.0. For SVM, LIBSVM v2.90-a freeware for training SVM classifier-was used. Empirical results showed that our proposed model based on SVM outperformed all the other comparative models in detecting network intrusions from the accuracy perspective. They also showed that our model reduced the total misclassification cost compared to the ANN-based intrusion detection model. As a result, it is expected that the intrusion detection model proposed in this paper would not only enhance the performance of IDS, but also lead to better management of FNE.

The relationship between condyle position, morphology and chin deviation in skeletal Class III patients with facial asymmetry using cone-beam CT (안면비대칭을 동반한 골격성 III급 부정교합자에서 하악 과두의, 위치 형태와 이부 편위의 관계: cone-beam CT를 이용한 연구)

  • Lee, Bo-Ram;Kang, Dae-Keun;Son, Woo-Sung;Park, Soo-Byung;Kim, Seong-Sik;Kim, Yong-Il;Lee, Kyung-Min
    • The korean journal of orthodontics
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    • v.41 no.2
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    • pp.87-97
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    • 2011
  • Objective: Facial asymmetry is usually evaluated from the difference in length and angulation of the maxilla and mandible. However, asymmetric position or shape of the condyle can also affect the expression of asymmetry. The purpose of this study was to evaluate the correlation between condylar asymmetry and chin point deviation in facial asymmetry. Methods: Cone-beam CT images of fifty adult skeletal Class III patients were studied. Thirty patients who had more than 4 mm menton deviation were categorized in the asymmetric group. Twenty patients with less than 4 mm menton deviation were assigned to the symmetric group. Anteroposterior and transverse condyle positions were evaluated from the cranial base. The greatest mediolateral diameter (GMD) of the condyle in the axial plane and angulation to the coronal plane were measured. The height and volume of the condyles were evaluated. Results: The symmetric group had no statistical difference between both condyles in position, angulation, GMD, height and volume. In the asymmetric group, the non-deviated side condyle was larger in GMD, height and volume than the deviated side. There was no statistical difference in condyle position and angulation. The GMD, height difference and condylar volume ratio (non-deviated/deviated) were positively correlated with chin deviation. From the linear regression analysis, condylar volume ratio was a significant factor affecting chin deviation. Conclusions: These findings suggests that the non-deviated side condyle is larger than the deviated side. In addition, condylar asymmetry can affect the expression of facial asymmetry.

Strategies of Local Terrestrial Broadcasting Companies since the Introduction of Comprehensive Programming Channels (종합편성채널 도입에 따른 지역 지상파방송 대응 전략)

  • Jeong, Jong-Geon
    • The Journal of the Korea Contents Association
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    • v.11 no.7
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    • pp.192-209
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    • 2011
  • This study looked into coping strategies of local terrestrial broadcasting companies in the media market, since the introduction of comprehensive programming channels was confirmed. Local terrestrial broadcasting companies vulnerable to market competitiveness are driven more out to the difficulty of survival in a limited advertising market with the advent of comprehensive programming channel. and Local terrestrial broadcasting receive discriminative application than comprehensive programming channel. They have reverse discrimination in must-carry, broadcasting area, programming regulation, advertisement regulation, broadcasting development fund. Hence, Local terrestrial broadcasting needs diverse countermeasures at difficult media circumstances. Above all, Competitive content reinforcement is desperately needed. That's why content distribution structure needs diversification. And, It is necessary for local terrestrial broadcasting companies to diversify the content distribution system. As a way to expand distribution structure of local broadcasting, the introduction of local programming regulations for total amount will be highlighted in the nationwide network program. The mandatory policy that programs produced by local terrestrial broadcasting companies will be broadcast regularly in prime time through a nationwide network is an example. In addition to developing high content independently, 2nd Multiple channels of distribution is needed. It has to be supplied to various platforms including local broadcasting, SO and etc. In addition, it is necessary to activate regional co-production program between local terrestrial broadcasting companies and regions. Time rate between central stations and local terrestrial broadcasting companies must be distributed practically. And also, local terrestrial broadcasting companies in addition to ad revenue model to themselves and their own businesses by expanding the sponsorship to strengthen the competitiveness will have to nurture self-sustaining. Moreover they must have enlarge economy of scale through widen of broadcasting area.