• Mycobiology
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• 제45권4호
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• pp.430-433
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• 2017

We characterized two endophyte fungi from the leaves of Astragalus membranaceus in Korea. The isolated strains were identified on the basis of the morphological characters and sequences analysis of the internal transcribed spacer and large subunit regions of the rDNA and ${\beta}$-tubulin gene. To the best of our knowledge, this is the first report of Diaporthe oncostoma and Diaporthe infecunda in Korea, and we have provided descriptions and figures.

• Natural Product Sciences
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• 제28권2호
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• pp.89-92
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• 2022

In Asia, the roots of Paeonia lactiflora and Astragalus membranaceus have been used as therapeutic agents for thousands of years. Once the medicinal plants are harvested, they are dried and their ingredients are extracted by heat-mediated reflux extraction. However, the condensed structure of organic products (especially roots) limits the extraction of bioactive components. In this study, we assessed the effect of the puffing method (using high temperature and pressure) before the extraction process in relation to the profile and antioxidant capacity of active ingredients. We demonstrated that the additional puffing process before extraction methods improves the yield of polyphenol concentrations and antioxidant activities from the roots of P. lactiflora and A. membranaceus.

• 한국식품과학회지
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• 제35권5호
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• pp.818-824
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• 2003

외국농산물의 국내 유입증가와 이에 따른 신속한 원산지 판별법 확립이 요구되는 가운데 최근 수입이 급증한 품목 중 하나인 황기를 선택, CE 및 NIRS를 이용하여 분석조건을 확립하고 원산지판별에의 적용 가능성을 검토하였다. CE를 이용하여 분석 시 추출은 methanol: 0.1M phosphate buffer(pH 2.5)(3:7)를 사용하였으며 uncoated fused silica capillary$(50\;{\mu}m\;I.D.{\times}27cm)$를 이용하여 $45^{\circ}C$, 14 kV로 분석, 200 nm에서 검출하였다. 분석 buffer는 0.1 M phosphate buffer(pH 2.5)에 20% methoxy ethanol과 40 mM HSA를 첨가하여 사용하였으며, 8초간 pressure injection 하였다. Peak의 재현성을 증대시키기 위하여 시료 주입 전 분석 buffer를 1분간 분석 시와 같은 방향으로(F) 흘려주고 0.1 M phosphoric acid와 1 M sodium hydroxide는 각각 4분, 5분간 반대방향으로(R) 홀려주었다. 증류수를 다시 1분간 흘려주고(R) 분석 buffer로 2분간 평형화(F) 시킨 후 시료를 주입하였다. 이상의 조건으로 국내산(97점)과 수입(113점) 황기를 분석한 결과 전체 peak의 양상은 유사하였으나 약 $11{\sim}13$분에 용출되는 2개의 peak(peak am-1, am-2)의 면적 비율에서 차이가 나타나 국산은 peak am-2가 peak am-1의 약 4배인 반면, 수입 산은 10배로 나타나 원산지 판별이 가능하였으며, 약 80%의 편별율을 나타내었다. NIRS는 국산 및 수입산 황기 raw 스펙트럼의 2차 미분 스펙트럼 R값이 0.915로 비교적 안정된 값을 얻을 수 있었고, SEP는 약 14.3%로 나타났다. 이를 국산과 수입산 황기에 적용 시 전체 판별율이 약 97%로 비교적 높은 판별율을 보였다. 또한 NIRS로 판별이 불가능한 시료가 CE로는 판별이 가능하여 이 두 기기를 함께 사용 시 상호보완하여 신속 정확한 원산지 판별법의 개발 가능성이 시사되었다.

• 한방재활의학과학회지
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• 제15권2호
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• pp.91-104
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• 2005

Objectives : This study was performed to investigate the effect of herbal acupuncture(Angelica gigas, Astragalus membranaceus) at Chun-wan(CV-12) and Chok-Samni(ST36) on weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and HDL-cholesterol to total cholesterol ratio of rats fed high fat diet for diet for 5 weeks. Methods : Experimental groups were divided into normal group, high fat diet group(Control), high fat diet and Angelica gigas herbal acupuncture at CV12 and ST36 treated group(HA-1), high fat diet and Astragalus membranaceus herbal acupuncture at CV12 and ST36 treated Group(HA-2), high fat diet and Astragalus membranaceus herbal acupuncture at CV12 and ST36 treated Group(HA-3). Herbal acupuncture was bilaterally treated at the level of 16.7 ul/kg body weight per day. Results : Body weight were significantly decreased on 15th and 20th day in HA-1, HA-2, and decresed on 25th and 30th day in all the groups. Food intake were increased on 20th day and after were decreased on 30th day in HA-1 and HA-2. And food intake of HA-3 were decreased on 10th day and after were increased on 20th and 25th day. Total cholesterol, LDL-cholesterol, triglyceride and ALT were significantly decreased in HA-1, HA-2 and HA-3 groups compared with control group. HDL-cholesterol to total cholesterol ratio were significantly increased in HA-1, HA-2 and HA-3 groups compared with control group. And food efficiency were significantly decreased in HA-1 and HA-3 groups compared with control group. Especially HDL-cholesterol were increased in HA-1 Group. Conclusions : Herbal acupuncture using Angelica gigas and Astragalus membranaceus at CV12 and ST36 can manage obesity by controlling body weight, food intake and food efficiency ratio.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2000년도 춘계 학술대회지
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• pp.158-159
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• 2000

• 한국작물학회:학술대회논문집
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• 한국작물학회 1997년도 추계 학술대회지
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• pp.118-119
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• 1997

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.258.3-258.3
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• 2003

The Seeds of Astragalus sinicus(Leguminosae) is used for the treatment of excretion of urine, circulation of blood and the throat inflammation in Korean traditional medicine. Eight flavonoids were isolated from 80％ Acetone extracts. The structure were elucidated as Quercetin 3-O-${\beta}$-D-Xylopyranosyl-(1\longrightarrow2)-${\beta}$-D-galactopyranoside, ampelopsin and myricetin by phytochemical and spectral evidences. The other compounds are understudied by 2D-NMR.

• 양봉협회회보
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• 통권344호
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• pp.63-63
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• 2009

• 한국약용작물학회지
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• 제19권2호
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• pp.83-89
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• 2011

This studies were conducted to investigate the growth responses and yields of Astragalus membranaceus Bunge which were grown with organic cultivation using two organic fertilizers and two green manure crops of hairy vetch and rye in 2008 and 2009. The lengths of stems and roots were not clearly different between organic and conventional cultivations. The root diameters and the branch numbers were slightly thicker and more in all treatments of organic cultivation than that of conventional cultivation in 2008, although they were not significantly different between two cultivating methods in 2009. The dry weights of shoot and root were heavier on organic cultivation than those on conventional cultivation. The T/R ratios were commonly higher in organic culture, showing more clearly in 2008 than 2009. The yields were generally higher in organic cultivation than those in conventional cultivation in 2008 although the effect did not clear on yield in 2009. The organic cultivation using of organic fertilizers and green manure crops would be new method to have safety and qualitative products. The techniques of organic cultivation for Astragalus membranaceus Bunge would be studied more on utilizing the natural organic resources.

• 셀메드
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• 제3권2호
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• pp.15.1-15.5
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• 2013

Previous reports showed that Compound Astragalus and Salvia miltiorrhiza extract (CASE), which was mainly composed of astragalosides, astragalus polysaccharide and salvianolic acids, inhibited hepatic fibrosis by mediating transforming growth factor-${\beta}$ (TGF-${\beta}$)/Smad signaling. Our aim was to examine the effects of CASE on D-galactosamine (D-GalN) treated liver injury in mice and carbon tetrachloride ($CCl_4$)-induced liver fibrosis in rats. CASE was administered to mice with D-GalN-induced liver injury and to rats with $CCl_4$-induced liver fibrosis, respectively. Liver injury was routinely evaluated by relative liver weight, serum levels of ALT, AST, hyaluronic acid (HA), hepatic malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, hydroxyproline (HYP) and histopathologic changes. Treatment of mice with CASE (60, 120, and 240 mg/kg, ig) significantly lowered ALT, relative liver weight, and MDA levels when compared with D-GalN treated mice. CASE (120, 240 mg/kg) significantly lowered ALT, AST, HA, HYP, and MDA levels against $CCl_4$ treated rats. Decreased SOD level was reversed with CASE treatment. Upon histopathological examination, CASE treatment had significantly inhibitory effect on the progression of hepatic fibrosis in rats. These results indicate that CASE might be effective in treatment and prevention of acute and chronic hepatic injury due to its antioxidant activity.