CELLMED (셀메드)

Cellmed Orthocellular Medicine and Pharmaceutical Association (셀메드 세포교정의약학회)
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Effects of compound traditional Astragalus and Salvia Miltiorrhiza extract on acute and chronic hepatic injury

  • Zhang, Xiaoxiang (Department of Pharmaceutical Engineering, Hefei University of Technology) ;
  • Yang, Yan (Department of Pharmacology and Institute of Natural Medicine, Anhui Medical University) ;
  • Liu, Xin (Therapeutics Research Centre, University of Queensland, Princess Alexandra Hospital) ;
  • Wu, Chao (Department of Pharmacology and Institute of Natural Medicine, Anhui Medical University) ;
  • Chen, Minzhu (Department of Pharmacology and Institute of Natural Medicine, Anhui Medical University)
  • Received : 2013.02.05
  • Accepted : 2013.05.20
  • Published : 2013.05.31
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Abstract

Previous reports showed that Compound Astragalus and Salvia miltiorrhiza extract (CASE), which was mainly composed of astragalosides, astragalus polysaccharide and salvianolic acids, inhibited hepatic fibrosis by mediating transforming growth factor-${\beta}$ (TGF-${\beta}$)/Smad signaling. Our aim was to examine the effects of CASE on D-galactosamine (D-GalN) treated liver injury in mice and carbon tetrachloride ($CCl_4$)-induced liver fibrosis in rats. CASE was administered to mice with D-GalN-induced liver injury and to rats with $CCl_4$-induced liver fibrosis, respectively. Liver injury was routinely evaluated by relative liver weight, serum levels of ALT, AST, hyaluronic acid (HA), hepatic malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, hydroxyproline (HYP) and histopathologic changes. Treatment of mice with CASE (60, 120, and 240 mg/kg, ig) significantly lowered ALT, relative liver weight, and MDA levels when compared with D-GalN treated mice. CASE (120, 240 mg/kg) significantly lowered ALT, AST, HA, HYP, and MDA levels against $CCl_4$ treated rats. Decreased SOD level was reversed with CASE treatment. Upon histopathological examination, CASE treatment had significantly inhibitory effect on the progression of hepatic fibrosis in rats. These results indicate that CASE might be effective in treatment and prevention of acute and chronic hepatic injury due to its antioxidant activity.

Keywords

References

  1. Hu S, Chen S, Li X, Qin R, Mei Z. Antitumor effects of Chi-Shen extract from Salvia miltiorrhiza and Paeoniae radix on human hepatocellular carcinoma cells. Acta Pharmacol Sin. 2007;28:1215-1223 https://doi.org/10.1111/j.1745-7254.2007.00606.x
  2. Huang XZ, She SF, Wang GB. Experimental research of the various doses treatment groups of Salvia miltiorrhiza to prevent liver fibrosis induced with $CCl_4$. Integr. Tradi and West Met J Hepatol. 2001;11:213-215.
  3. Iredale JP, Benyon RC, Pickering J, McCullen M, Northrop M, Pawley S, Hovell C, Arthur MJ. Mechanisms of spontaneous resolution of rat liver fibrosis. Hepatic stelllate cell apoptosis and reduced hepatic expression of metalloproteinase inhibitors. J Clin Invest. 1998;102:538-549. https://doi.org/10.1172/JCI1018
  4. Kang H, Ahn KS, Cho C, Bae HS. 2004. Immunomodulatory effect of Astragali Radix extract on murine TH1/TH2 cell lineage development. Biol Pharm Bull. 2004;27:1946-1950. https://doi.org/10.1248/bpb.27.1946
  5. Karadeniz A, Cemek M, Simsek N. The effects of Panax ginseng and Spirulina platensis on hepatotoxicity induced by cadmium in rats. Ecotoxicol Environ Saf. 2009;72:231-235. https://doi.org/10.1016/j.ecoenv.2008.02.021
  6. Li C, Luo J, Li L, Cheng M, Huang N, Liu J, Waalkes MP. The collagenolytic effects of the traditional Chinese medicine preparation, Han- Dan-Gan-Le, contribute to reversal of chemical- induced liver fibrosis in rats. Life Sci. 2003;72:1563-1571. https://doi.org/10.1016/S0024-3205(02)02448-7
  7. Lin YL, Wu CH, Luo MH, Huang YJ, Wang CN, Shiao MS, Huand YT. In vitro protective effects of salvianolic acid B on primary hepatocytes and hepatic stellate cells. J Ethnopharmacol. 2006;105:215-222. https://doi.org/10.1016/j.jep.2005.10.021
  8. Liu J, Shen H, Ong CN. Salvia miltiorrhiza inhibits cell growth and induces apoptosis in human hepatoma HepG2 cells. Cancer Letters 2000;153:85-93 https://doi.org/10.1016/S0304-3835(00)00391-8
  9. Liu X, Yang Y, Zhang X, Xu S, He S, Huang W, Roberts MS. Compound Astragalus and Salvia miltiorrhiza extract inhibits cell invasion by modulating transforming growth factor-b/Smad in HepG2 cell. J Gastroenterol Hepatol. 2010;25:420-426. https://doi.org/10.1111/j.1440-1746.2009.05981.x
  10. Roxas M, Jurenka J. Colds and influenza: a review of diagnosis and conventional, botanical, and nutritional considerations. Altern Med Rev. 2007;12:25-48.
  11. Shyu MH, Kao TC, Yen GC. Hsian-tsao (Mesona procumbens Heml.) prevents against rat liver fibrosis induced by $CCl_{4}$ via inhibition of hepatic stellate cells activation. Food Chem Toxicol. 2008;46:3707-3713. https://doi.org/10.1016/j.fct.2008.09.051
  12. Stachlewitz RF, Seabra V, Bradford B, Bradham CA, Rusyn I, Germolec D, Thurman RG. Glycine and uridine prevent D-galactosamine hepatotoxicity in the rat: role of Kupffer cells. Hepatology. 1999;29:737-745. https://doi.org/10.1002/hep.510290335
  13. Tsukamoto H, Matsuoka M, French SW. Experimental models of hepatic fibrosis: a review. Semin Liver Dis. 1990;10:56-65. https://doi.org/10.1055/s-2008-1040457
  14. Wu Q, Yang Y, Xue S, Zhang X, Zhou Y, Chen M. Effect of astragalosides on proliferation and collagen production of hepatic stellate cells in vitro. Chin Pharmacol Bull. 2003;19:892-895.
  15. Yang Y, Yang S, Chen M, Zhang X, Zou Y, Zhang X. Compound Astragalus and Salvia miltiorrhiza Extract exerts anti-fibrosis by mediating TGF-beta/Smad signaling in myofibroblasts. J Ethnopharmacol. 2008;118:264-270. https://doi.org/10.1016/j.jep.2008.04.012
  16. Zhang XX, Yang Y, Chen MZ. Effect of total polysaccharide of Astragalus on proliferation and collagen production of HSC-T6 cells. Chin. J. Clin. Pharmacol. Ther (Chin). 2003;8:645-647.

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