• The Journal of Internal Korean Medicine
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• v.27 no.3
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• pp.579-588
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• 2006

Objective : This study was performed to investigate the effect of Astragali Radix on leukocytopenia anaphysis with carcinostatic substance elicitation. Methods : We observe if there were differences in a general tendency by EAR (AR extracts) capacity in the Astragali Radix treated group and the cyclophosphamide treated group, a general tendency of recovery from leukocytopenia over lime and checked for liver and kidney toxicity. Results : In cyclophosphamide treated with EAR 1000mg/kg group, the recovery rate of leukocytopenia increased and there were no differences in liver and kidney functions. Conclusions : Astragali Radix was effective and safe in increasing rate of leukocytopenia anaphysis with carcinostatic substance elicitation and indicated possibility for clinically adaptation.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.4
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• pp.1054-1058
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• 2003

In order to evaluate the direct effect of estrogenic compounds in oriental herbal medicines, the estrogenic activity was measured using an in vitro detection system. For this system, human breast cancer cell line MCF7 was transfected using an estrogen responsive CAT reporter plasmid. Estrogenic activities of Platycodi radix, Astragali radix and Glycyrrhizae radix were evaluated using this system. Estrogenic activity of a 500 ㎍/ml ethanol extract of Platycodi radix was as same as that of a 10/sup -8/ M standard solution (17β-estradiol) and activity of a 50 ㎍/ml ethanol extract was between those of a 10/sup -8/ M and a 10/sup -9/ M standard solutions. In addition, estrogenic activity of a 50 ㎍/ml ethanol extract of Platycodi radix was as same as that of a 10/sup -10/ M standard solution. The same activity patterns were observed in the system which was treated by Astragali radix or Glycyrrhizae radix extracts. The most effective activity was observed in a system which was treated by Platycodi radix extract, but the least activity was observed by Glycyrrhizae radix extract. In this result, it was confirmed that Platycodi radix, Astragali radix and Glycyrrhizae radix extracts possess estrogenic compounds.

• YAKHAK HOEJI
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• v.44 no.4
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• pp.371-377
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• 2000

In order to investigate the pharmacological properties of fractions of Astragali Radix and Polygalae Radix, the effects of the fractions on cerebral ischemia and subsequent reperfusion were studied. Brain ischemia was induced by bilateral common carotid artery occlusion in mongolian gerbil. Brains were recirculated for 30 mins after the 20 min occlusion. Methanol and butanol fractions of Astragali Radix and Polygalae Radix were administered orally 2 hrs before common carotid artery occlusion. Histological observations showed that brain ischemia induced severe brain damage evidenced by the presence of necrotic foci, edema and hemorrhage. This injury was prevented by the methanol fraction and butanol fraction of Polygalae Radix. The level of ATP in brain tissue significantly decreased in ischemic gerbils. This decrease was prevented by the pretreatment with butanol fraction of Polygalae Radix. In contrast, the levels of lactate and lipid peroxide were both elevated in ischemic gerbils. This elevation was inhibited by the pretreatments with methanol fraction and butanol fraction of Polygalae Radix. Our findings suggest that the Polygalae Radix improves ischemia-induced brain damage.

• The Korea Journal of Herbology
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• v.23 no.2
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• pp.111-121
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• 2008

Objectives : The present study was carried out to investigate the effects of Astragali radix on the asthma treatment. EAR-I is an extract of astragali radix cultured for one year and EAR-III is an extract of astragali radix cultured for three years. Methods : Two asthma-induced groups mice group was treated respectively with EAR- I and EAR- III. The other group was not. Each group was analyzed in vivo and in vitro experiments. Results : In asthma-induced mice by OVA treatment, the weight of lung, total cell number of leukocyte and eosinophil in BALF, both EAR- I and EAR- III treated groups were significantly decreased compared to control group. IL-4, IL-5, IL-13, histamine, IgE in BLAF of group treated with both EAR-I and EM-III were significantly decreased compared to control group. In FACS analyzing, granulocytes, $CD3e^-$/$CCR3^+$, $CD3^+$/$CD19^$, $CD3e^+$/$CD69^+$, $CD4^+$, $CD8^+$ and $GR-1^+$/$CD11b^+$ were significantly increased in asthma induced mice group by OVA treatment compared to control group, and decreased in group treated with both EAR- I and EAR-III. Conclusions : The present data proves that extract of astragali radix has an effect on the inhibiting asthma. Moreover, astragali radix cultured for three years was proved to be superior to the one cultured for one year.

• The Journal of Korean Medical History
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• v.28 no.2
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• pp.47-58
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• 2015

Objectives : The purpose of this study was to investigate Wang Lun (王綸)'s assertion on Ginseng and Astragali Radix and its related arguments that had shown critical or advocative point of views about Wang lun's theory. It was expected that this study would help understand the history of arguments on the usage of Ginseng and Astragali Radix that had been triggered by Wang lun's assertion. Methods : In the process of investigation, this study first looked into the assertions of Wang Lun and the representative arguments about it, which includes Yu Tuan, Wang Ji (汪機), Sun Yikui (孫一奎). And secondly, this study also examined the other following additional arguments that had shown the conclusional aspects of this disputes. Results & Conclusions : Wang Lun (王綸)'s assertion on Ginseng and Astragali Radix proclaimed that in the symptoms of fevers in lung or lack of blood (血) the use of Ginseng and Astragali Radix must be prohibited. This assertion later faced severe criticism by opposers like Yu Tuan, Wang Ji. These opposers asserted that Yang (陽) has the ability of creating Yin (陰), and Ginseng and Astragali Radix could be the right drugs to the symptoms by lack of Yin. But a few approvers like Sun Yikui (孫一奎) had tried to advocate Wang Lun's assertion and casted the question of true meaning of Wang Lun. And In the other following arguments, it could be said that the influence of Wang Lun's assertion had gradually waned but could have been reciprocally accepted in the opposite point of view.

• The Korea Journal of Herbology
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• v.21 no.1
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• pp.17-24
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• 2006

Objectives : This study was conducted to compare antioxidant activity of ratio-related Angelicae Radix and Astragali Radix compositions by DPPH radical scavenging activity. Methods : The species of Angelicae Radix was compared as Angelica gigas, A. sinensis and A. acutiloba. Then various compositions of Angelicae Radix with Astragali Radix were prepared with different ratio. Diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity was measured after 10, 20 and 30 minutes. Each extracts were tested by 1, 5, 10, 50, 100, 500 and 1,000 ${\mu}g/ml$ concentrations. Results : The results showed that Angelica gigas, A. sinensis and A. acutiloba scavenged DPPH radical with 66.8, 61.7 and 56.7% in 1,000 ${\mu}g/ml$ concentration, respectively. According to this result, Angelicae Radix and Astragali Hadix compositions with 100:0, 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, 1:10 and 0:100 ratio were scavenged DPPH radical with 56.3, 49.9, 61.2, 55.7, 54.7, 55.0, 52.8 ,md 50.2% in 1,000 ${\mu}g/ml$ concentration, respectively. The extract of Angelicae Radix and Astragali Radix compositions with 100:0, 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, 1:10 and 0:100 ratio scavenged DPPH radical with the $IC_{50}$ being 85,21, 1016.26, 656.51, 785.67 and 956.74 ${\mu}g/ml$ respectively. Conclusion : In conclusion, the extract of Angelicae Radix and Astragali Radix compositions with 1:1 ratio showed dose-dependent DPPH radical scavenging activity.

• Journal of Pharmacopuncture
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• v.3 no.2
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• pp.79-97
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• 2000

Objectives : To investigate the effects of herb-acupunctures of Qi tonification herbs or formula on the rat immune depression induced by an anticancer drug, methotrexate (MTX) Methods: Animals were divided into 5 groups; Normal control group was not given any treatment. Immune depression was induced by oral administration of 1mg/kg MTX b.i.d for 4 days in Control, Sample I, II, and III groups. We treated on CV 4 (Guanyuan) with saline, Bojoongiggi-tang (Buzhongyiqi-tang), Ginseng Radix and Astragali Radix herb-acupunctures in Control, Sample I, II, and Ill groups, respectively. In both blood and spleen, the assessment of CD4+ T-cell count, CD8+ T-cell count, CD4/CD8 T-cell ratio was performed. Results: Here we show that 3 herb- acupuncture groups have an influence, to some extent, on CD4+ and CD8+ T-cell counts and CD4/CD8 T-cell ratio in both blood and spleen. Astragali Radix herb-acupuncture, in particular, was found to have significantly increased CD4+ T-cell count in blood and CD4/CD8 T-cell ratio in blood and spleen. Conclusions: The results of this study suggest that herb-acupunctures of Bojoongiggi-tang (Buzhongyiqi-tang), Ginseng Radix and Astragali Radix may have an influence over rat immune depression induced by MTX.

• Korean Journal of Pharmacognosy
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• v.48 no.3
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• pp.248-254
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• 2017

A simple and reliable reverse phase HPLC method was developed to determine pharmacologically active marker compounds of Astragali Radix, Paeoniae Radix, and Corni Fructus. The stability test of water-extract from three natural medicines were examined for six months. However, no significant changes in the content of the marker compounds of each extract were observed during the investigation.

• The Journal of Internal Korean Medicine
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• v.26 no.2
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• pp.291-301
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• 2005

Objective : Anticancerous and host safety effects of the combination of Astragali Radix and chlorambucil are studied when it is applied to leukemia cell(P388D1 cell) related disease. Methods : After 5 groups of mice are treated by respective procedures (HG, CHL, HG+CHL, etc), the quantitative analyses (cell proliferation assay, mutagenesis test, survival rate, weight shift observation, tissue and blood analyses, etc) are conducted. Results : While sole injection of Astragali Radix extends the survival period and deter the liver and marrow function deterioration, combined injection (HG+CHL) shows more strong anticancerous effect than sole injection of Astragali Radix or Cholrambucil, and also does not cause any mutation on the normal cells and virulence on marrow and liver caused by leukemia. Conclusions : The combined application of Astragali Radix and chlorambucil has more effective anticancerous and host safety effects than independent use of them.

• Journal of Acupuncture Research
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• v.29 no.2
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• pp.59-71
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• 2012

Objectives : The purpose of this study was to examine the effects of the Astragali radix Pharmacopuncture on improvement of senile osteoporosis (Type 2) in SAM P6. Methods : At 10 weeks after birth, Astragali radix Pharmacopunctures were given 100mL, 200mL/kg/day, i.p. 3times a week for 4 weeks at $CV_{12}$ in SAM P6 mice. We measured complete blood cells (CBC) such as RBC, HGB, Hct, PLT, MPB and MCHC. And we analyzed the plasma concentrations of blood urea nitrogen, creatinine, inorganic phosphate and total iron. In addition, we tested bone mineral density (BMD) using the soft X-ray. Results : The results were as follows. 1. At $CV_{12}$ in SAM P6, treatments of Astragali radix Pharmacopuncture were showed a trend of increase in bone mineral density (BMD) of the vertebrae lumbales, femurand tibia in P6 mice. 2. At $CV_{12}$ in SAM P6, treatment of Astragali radix Pharmacopuncture increased in RBC, HB, HCT and PLT, in comparison with control group. It was also found that the inorganic phosphate levels increased in the treatment on groups of the Astragali radix Pharmacopuncture from that of the control group, but blood urea nitrogen was no significant. Conclusions : These results are suggested that at $CV_{12}$ the Astragali radix Pharmacopuncture help on improvement of osteoporosis in SAMs.