• Journal of Life Science
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• v.30 no.1
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• pp.88-95
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• 2020

Using a random arbitrarily primed polymerase chain reaction, messenger RNA expression levels were assessed after exposure to 10% (v/v) toluene for 8 hr in solvent-tolerant Pseudomonas sp. BCNU 106. Among the 100 up-expressed products, 50 complementary DNA fragments were confirmed to express repeatedly; these were cloned and then sequenced. Blast analysis revealed that toluene stimulated an adaptive increase in the gene expression level in association with transcriptions such as LysR family of transcriptional regulators and RNA polymerase factor sigma-32. The expression of catalase and Mn2+/Fe2+ transporter genes functionally associated with inorganic ion transport and metabolism increased, and the increased expression of type IV pilus assembly PilZ and multi-sensor signal transduction histidine kinase genes, functionally categorized into signal transduction and mechanisms, was also demonstrated under toluene stress. The gene expression level of beta-hexosaminidase in association with carbohydrate transport and metabolism increased, and those of DNA polymerase III subunit epsilon, DNA-3-methyladenine glycosylase II, DEAD/DEAH box helicase domain-containing protein, and ABC transporter also increased after exposure to toluene in DNA replication, recombination, and repair, and even in defense mechanism. In particular, the RNAs corresponding to the ABC transporter, Mn2+/Fe2+ transporter, and the β-hexosaminidase gene were confirmed to be markedly induced in the presence of 10% toluene. Thus, defense mechanism, cellular ion homeostasis, and biofilm formation were shown as essential for toluene tolerance in Pseudomonas sp. BCNU 106.

• Journal of Ginseng Research
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• v.44 no.2
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• pp.312-320
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• 2020

Background: Ginseng (Panax ginseng Meyer) is an essential source of pharmaceuticals and functional foods. Ginseng productivity has been compromised by high light (HL) stress, which is one of the major abiotic stresses during the ginseng cultivation period. The genetic improvement for HL tolerance in ginseng could be facilitated by analyzing its genetic and molecular characteristics associated with HL stress. Methods: Genome-wide analysis of gene expression was performed under HL and recovery conditions in 1-year-old Korean ginseng (P. ginseng cv. Chunpoong) using the Illumina HiSeq platform. After de novo assembly of transcripts, we performed expression profiling and identified differentially expressed genes (DEGs). Furthermore, putative functions of identified DEGs were explored using Gene Ontology terms and Kyoto Encyclopedia of Genes and Genome pathway enrichment analysis. Results: A total of 438 highly expressed DEGs in response to HL stress were identified and selected from 29,184 representative transcripts. Among the DEGs, 326 and 114 transcripts were upregulated and downregulated, respectively. Based on the functional analysis, most upregulated and a significant number of downregulated transcripts were related to stress responses and cellular metabolic processes, respectively. Conclusion: Transcriptome profiling could be a strategy to comprehensively elucidate the genetic and molecular mechanisms of HL tolerance and susceptibility. This study would provide a foundation for developing breeding and metabolic engineering strategies to improve the environmental stress tolerance of ginseng.

• International Journal of Automotive Technology
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• v.8 no.6
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• pp.713-722
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• 2007

Design variables for suspension systems cannot always be realized in the actual suspension systems due to tolerances in manufacturing and assembly processes. In order to deal with these tolerances, design variables associated with kinematic configuration and compliance characteristics of suspensions are treated as random variables. The reliability of a design target with respect to a design variable is defined as the probability that the design target is in the acceptable design range for all possible values of the design variable. To compute reliability, the limit state, which is the boundary between the acceptable and unacceptable design, is expressed mathematically by a limit state function with value greater than 0 for acceptable design, and less than 0 for unacceptable design. Through reliability analysis, the acceptable range of design variables that satisfy a reliability target is specified. Furthermore, through sensitivity analysis, a general procedure for optimization of the design target with respect to the design variables has been established.

• Journal of the Korean Society of Safety
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• v.25 no.5
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• pp.7-14
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• 2010

Ultra high pressure system, which can be generally increased over 1,000bar, needs to have sealing mechanism to protect leakage and selection of the materials used in the intensifier. Components such as pressure vessel, hydraulic hose assembly, accumulator, hydraulic cylinder, hydraulic valve, pipe, etc., are tested under the impulse-pressure conditions. Components need to be tested under 1.5 to 3 times of rated pressure to check the tolerance even though rated pressure range of these components are not ultra high pressure. So, the ultra high pressure system needs to be equiped to test components. In this study, safety assessments of ultra high pressure system which are using failure analysis of components, changing the types of the control system, and finite element analysis with static condition, are investigated.

• Transactions of the Korean hydrogen and new energy society
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• v.18 no.4
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• pp.439-445
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• 2007

Proton exchange membrane Fuel Cells(PEMFCs) have been spotlighted because of their broad potential application for potable electrical devices, automobiles and residential usages. However, their utilization is limited to low temperature operation due to the electrolyte dehydration at high temperature. High temperature PEMFC operation offers high CO tolerance and easy water management. This review presents development of high temperature($120{\sim}200^{\circ}C$) PEMFC. Especially, PEMFC which is based on acid-doped PBI membrane is discussed.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.26 no.9
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• pp.1727-1735
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• 2002

The usage of beam scanning type laser marker is rapidly increasing in the field of semiconductor equipment. A device called ″beam adjuster″ is employed to adjust the visible diode laser, which points the marking position for various setting. The device is very sensitive to manufacturing tolerance and assembly condition. Axiomatic approach has been applied to the design of the device. An existing design is analyzed based on the Independence Axiom. The existing design is found to violate the axiom. Two new designs are proposed to satisfy the Independence Axiom. The Information Axiom is utilized to evaluate the designs. A design is selected to have the minimum information content. The significance of this research is that a full cycle of axiomatic design is applied to a real engineering product.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.12 no.6
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• pp.64-70
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• 2003

A measurement of spacecraft alignment is an important process of spacecraft assembly, integration and test because it is necessary that a ground station controls the precise positions of on-orbit spacecraft by using the alignment data of attitude orbit control sensors(AOCS) on spacecraft. In addition, accuracy of spacecraft alignment requirement is about $0.1^{\circ}$~$0.7^{\circ}$. The spacecraft alignment is measured by autocollimation of theodolite. This paper describes the measurement principle and method of spacecraft alignment. The result shows that all of the AOCS on the spacecraft are aligned within the tolerance required through the alignment measurement.

• Journal of Institute of Control, Robotics and Systems
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• v.19 no.11
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• pp.968-973
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• 2013

In the transportation industry, especially in the shipbuilding process, 3D surface measurement of large-scale hull pieces is needed for fabrication and assembly. We suggest an efficient method for checking the shape of curved plates under the forming operation with short time by measuring 3D profiles along the multi lines of the target surface. For accurate profile reconstruction, 2D camera calibration and 3D calibration using gauge blocks were performed. The evaluation test shows that the measurement accuracy is within the boundary of tolerance required in the shipbuilding process.

• Journal of the Korean Society for Precision Engineering
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• v.32 no.1
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• pp.83-88
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• 2015

At the early stages of engineering design, a number of design concepts may be suggested. With a great degree of freedom, making a good choice may be a challenge at this stage. In this study, a simplified evaluation criterion for design concepts is presented based on cost, simplicity and safety. Cost is estimated by the amount of materials and the required level of tolerance. Simplicity is represented by the number of parts, assembly steps, and fasteners. Safety is assessed by the level of potential failure. The proposed criterion is applied to an example design of driving mechanisms for cervical vertebrae massage machine.

• Journal of Ecology and Environment
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• v.40 no.1
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• pp.55-65
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• 2016

Background: In this article, it was analyzed how snow melting affects the assembly of lichen and moss communities in a small area of the coastal region of Barton Peninsula, which is in maritime Antarctic. In the small area, even though there is a huge gap of difference of the environment between the snow-filled area and snow-melt one, the latter did not have distinctive environmental gradients. Results: Depending on the snow melting time, coverage and species diversity of lichens and mosses tend to increase remarkably. For species with significant changes depending on the snow-covered period, there are Andreaea regularis, crustose lichens, Placopsis contortuplicata, Usnea aurantiaco-atra, and snow algae. In this area, the process of vegetation assembly process has shown the directional development in the order of snow algae${\rightarrow}$crustose, lichen sub-formation${\rightarrow}$fruticose lichen, moss cushion sub-formation (Andreaea sociation)${\rightarrow}$fruticose lichen, and moss cushion sub-formation (Usnea sociation), according to the order of snow melting. These directional development stages are shown in gradual change in small area with the snow melting phenomena. However, in the snow-free area, where water is sufficiently supplied, it is expected that moss carpet sub-formation (Sanionia sociation) will be developed. Vegetation development in the small area with the snow melting phenomena, depending on differences of resistance on snow kill and moisture settled by species in according to the time of snow melting, tolerance model to form community is followed. Conclusions: The research results explain the development of vegetation in the Antarctic tundra and its spatial distribution according to the period for growth of lichens and mosses in the summer time by differences of snow melting in the small area. In the future, if research for the community development process in a large scale will be done, it will be helpful to figure out temporal and spatial dynamic of vegetation in the Antarctic tundra where snow and glaciers melt rapidly due to climatic warming.