• 한국경영과학회:학술대회논문집
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• 한국경영과학회 2008년도 추계학술대회 및 정기총회
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• pp.191-196
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• 2008

This paper presents an evolutionary algorithm for goal programming with preemptive priority. To do this, an evolutionary strategy is suggested which search for the solution satisfying the goals in the order of the priority. Two-sided assembly line balancing problems with multiple goals are used to validate the applicability of the algorithm. In the problems, three goals are considered in the following priority order: minimizing the number of mated-stations, achieving the goal level of workload smoothness, and maximizing the work relatedness. The proper evolutionary components such as encoding and decoding method, evaluation scheme, and genetic operators, which are specific to the problem being solved, are designed in order to improve the algorithm's performance. The computational result is reported.

• Journal of Information Technology Applications and Management
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• 제9권3호
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• pp.13-30
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• 2002

ebXML (Electronic Business using extensible Markup language), sponsored by UN/CEFACT and OASIS, is a modular suite of specifications that enables enterprises of any size and in any geographical location to conduct business over the Internet. Using ebXML, companies now have a standard method to exchange business messages, conduct trading relationships, communicate data In common terms and define and resistor business processes. It is the mission of ebXML that provides an open XML-based infrastructure enabling the global use of electronic business information in an interoperable, secure and consistent manner by all parties for creating a single e1ectronic electronic market. This paper briefly overviews the concept of core component, context, assembly rule and context rule. Then, It designs by standard specifications of ebXML core component commonly used in an industry and among industries, and assembles business messages by using XML schema. Therefore, it suggests the mechanism which effectively exchanges business messages among the trading partners. This paper designs core component by using only three business messages of retail industry : orders, dispatch report, sales report.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 추계학술대회논문집A
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• pp.579-583
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• 2001

Cause of noise/vibration for the sheet feeding apparatus of digital MFP is investigated by analyzing the measured sound pressure and the strategy for reducing the noise level is suggested. Factors related to the noise/vibration are found by loading test and the measurement of the noise by assembling the components. By checking the center distance between gears and the boss height, precision of assembly is identified as a main factor of noise. From the result of analysis, it can be concluded that the noise level of sheet feeding apparatus can be reduced by maintaining the distance between gears and the precision of assembly adequately.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2002년도 춘계학술대회논문집
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• pp.554-558
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• 2002

A magnetostrictive sensor is used to measure stress waves propagating in a ferromagnetic cylinder without physical contact. The performance of a magnetostrictive sensor is affected most significantly by the bias magnetic field applied around the measurement location. The goal of this paper is to carry out the topology optimization of the bias magnet and yoke assembly to maximize the sensor output for traveling bending waves. We will use the multi-resolution topology optimization strategy to find the assembly of the bias magnet and the yoke that is easy to realize. The effectiveness of the present design is confirmed by an actual measurement of the sensor signal with the proposed bias magnet and yoke configuration.

• 기록학연구
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• 제14호
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• pp.241-288
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• 2006

오늘날 지방자치단체는 중앙정부가 추진하고 있는 지방분권에 대응하는 수단으로 로컬 거버넌스를 지향하고 있다. 로컬 거버넌스는 공사간의 구분없이 협력과 파트너십을 통해 문제의 해결을 도모한다는 점에서 무엇보다도 정보의 공유를 통한 각 행위주체 및 네트워크 형성 운영 자체의 투명성과 책임성이 중요한 과제임을 보여주고 있다. 그런데 이러한 정보의 공유를 위해서는 공유될 정보, 즉 기록이 수집, 관리되는 시스템을 구축하는 것이 전제되어야만 한다. 이런 배경 속에서 지방의회는 주민대표기관이자 전통적인 주민참여 집행기관(단체장) 견제기구로서 로컬 거버넌스를 구성하는 중요한 행위주체가 된다. 그럼에도 불구하고 지방의회에 대한 정보는 부존재하거나 비공개되고 있으며, 공개되는 정보 역시 그 양이나 질에 있어서 시민사회로부터 받는 불신과 저평가를 해소하기에 부족한 현실에 있다. 지방의회는 기록관리에 대한 전반적인 의식변화와 함께 기록관리의 근본적인 개선이 시급한 시점에 이른 것이다. 본 연구는 이러한 문제의식에 근거하여 오늘날 지방의회의 기록관리 실태를 검토하고 이에 대한 개선방안을 제안하였다. 기록은 생산되는 시점부터 관리까지의 모든 프로세스가 합리적인 구조 속에서 일관적으로 수행될 때 진정한 가치를 구현할 수 있다. 따라서 본 연구에서는 무엇보다도 지방의회에서 반드시 생산 관리되어야 하는 기록을 기록물분류기준표의 단위업무를 통해 제어할 수 있도록 하였으며, 처리과-기록관-전문관리기관이라는 세 단계로 구분된 우리의 기록관리체계에 부응하여 적용할 수 있는 기록관리정책 및 기록관의 설치를 제안하였다. 마지막으로, 지방의회가 기록관리에 의해 실현하는 투명성과 책임성의 확보와 더불어, 기록관리 자체로 로컬 거버넌스 실현의 유력한 수단이 되는 여러 효과에 대해 정리하였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2010년도 제39회 하계학술대회 초록집
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• pp.262-262
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• 2010

We introduce a novel and efficient strategy for producing free-standing functional films via photo-crosslinking and electrostatic layer-by-layer (LbL) assembly, which can allow the buildup of hydrophilic multilayers onto hydrophobic surfaces. Hydrophobic multilayers were deposited on ionic substrates by a photo-crosslinking LbL process using photo-crosslinkable polymers. The photo-crosslinked surface was converted to an anionic surface by excess UV light irradiation. This treatment allowed also the stable adhesion between metal electrode or cationic polyelectrolyte and hydrophobic multilayers. After dissolving the ionic substrates in water, the formed free-standing films exhibited unique functionalities of inserted components within hydrophobic and/or hydrophilic multilayers.

• Journal of Microbiology and Biotechnology
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• 제34권7호
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• pp.1544-1549
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• 2024

This study presents a fluorescent mechanism for two-step amplification by combining two widely used techniques, exponential amplification reaction (EXPAR) and catalytic hairpin assembly (CHA). Pseudomonas aeruginosa (P. aeruginosa) engaged in competition with the complementary DNA in order to attach to the aptamer that had been fixed on the magnetic beads. The unbound complementary strand in the liquid above was utilized as a trigger sequence to initiate the protective-EXPAR (p-EXPAR) process, resulting in the generation of a substantial quantity of short single-stranded DNA (ssDNA). The amplified ssDNA can initiate the second CHA amplification process, resulting in the generation of many double-stranded DNA (dsDNA) products. The CHA reaction was initiated by the target/trigger DNA, resulting in the release of G-quadruplex sequences. These sequences have the ability to bond with the fluorescent amyloid dye thioflavin T (ThT), generating fluorescence signals. The method employed in this study demonstrated a detection limit of 16 CFU/ml and exhibited a strong linear correlation within the concentration range of 50 CFU/ml to 10⁵ CFU/ml. This method of signal amplification has been effectively utilized to create a fluorescent sensing platform without the need for labels, enabling the detection of P. aeruginosa with high sensitivity.

• The Plant Pathology Journal
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• 제37권2호
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• pp.162-172
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• 2021

Soybean mosaic virus (SMV) is the predominant viral pathogen that affects the yield and quality of soybean. The natural host range for SMV is very narrow, and generally limited to Leguminosae. However, we found that SMV can naturally infect Pinellia ternata and Atractylodes macrocephala. In order to clarify the molecular mechanisms underlying the cross-family infection of SMV, we used double-stranded RNA extraction, rapid amplification of cDNA ends polymerase chain reaction and Gibson assembly techniques to carry out SMV full-length genome amplification from susceptible soybeans and constructed an infectious cDNA clone for SMV. The genome of the SMV Shanxi isolate (SMV-SX) consists of 9,587 nt and encodes a polyprotein consisting of 3,067 aa. SMV-SX and SMV-XFQ008 had the highest nucleotide and amino acid sequence identities of 97.03% and 98.50%, respectively. A phylogenetic tree indicated that SMV-SX and SMV-XFQ018 were clustered together, sharing the closest relationship. We then constructed a pSMV-SX infectious cDNA clone by Gibson assembly technology and used this clone to inoculate soybean and Ailanthus altissima; the symptoms of these hosts were similar to those caused by the virus isolated from natural infected plant tissue. This method of construction not only makes up for the time-consuming and laborious defect of traditional methods used to construct infectious cDNA clones, but also avoids the toxicity of the Potyvirus special sequence to Escherichia coli, thus providing a useful cloning strategy for the construction of infectious cDNA clones for other viruses and laying down a foundation for the further investigation of SMV cross-family infection mechanisms.

• 한국컴퓨터정보학회논문지
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• 제15권2호
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• pp.179-187
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• 2010

본 연구는 제품구조의 관점으로부터 접근하여 우리나라의 전자산업 및 자동차산업에 대한 경쟁력 제고 방안을 모색하고자 한다. 제품구조는 Modular(짜맞추기)형과 Integral(현장맞춤)형의 두 가지로 분류된다. 부품 간 특성으로서는 서브시스템 간의 독립성이 높은 것이 Modular형이고, 서브시스템 간에 높은 상호의존성이 있는 것이 Integral형이다. 제품구조의 차이에 의해서 그것에 적합한 상품개발의 조직능력이 다르다. 상품개발의 조직능력에는 크게 나누어서 '통합 현장맞춤 능력'과 '선택 짜맞추기 능력'이 있다. 자동차와 같은 Integral형 제품에는 부품 간이나 기업 간에서 현장맞춤 능력이 중요하며, PC와 같은 Modular형 제품에는 최적의 부품이나 기업을 선택하여 효과적으로 그것들을 짜맞추는 능력이 필요하다. 제품구조에 관한 '설계 제조전략'과 고객가치에 관한 '고객가치전략'을 통합함으로써 상품의 경쟁력 제고 전략을 추구한다.

• 산업경영시스템학회지
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• 제45권2호
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• pp.37-47
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• 2022

The Act on the Punishment of Serious Accidents to Prevent Large-scale Disasters, including Ferry Sewol and Taean Thermal Power Plant, passed the National Assembly on January 8, 2021, and has been in effect since January 27, 2022. However, the law, in which the representative of the headquarters is unlimitedly responsible for each worker's accident, is somewhat unreasonable at a time when a company owns dozens to hundreds of construction sites due to the nature of the construction industry. I agree with the purpose of enacting the law to reduce chronic serious accidents at construction sites, but it is necessary to carefully reconsider the implementation of the law in that punishment alone cannot achieve industrial safety. Previous studies focused on revising the Occupational Safety and Health Act, but there are few studies on the impact on the construction industry after the implementation of the Serious Accident Act. Therefore, this study attempts to derive problems related to the application of the Serious Accident Act and present improvement measures. To this end, after analyzing previous studies, SWOT analysis was performed by applying the Delphi method to derive strengths, weaknesses, opportunities, and threats. In addition, the results of two surveys of safety experts such as public institutions, academia, and companies were reflected, and its countermeasures were presented as follows. S/O strategy: establishing on-site execution capabilities of health and safety management system; W/O strategy: expanding legal and system execution checks; S/T strategy: establishing a risk response system; W/T strategy: expanding consulting by external specialized institutions