• Korean Journal of Plant Resources
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• v.9 no.2
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• pp.183-188
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• 1996

Chinese crude drug 'Se Sin'(細辛)has been used to cure headache, cough and expectorant. To the botanical origin of 'Se Sin', Zhong-yao-zhi(中葯志) reported Asarum heterotropoides var. mandshuricum. A. sieboldii and A. sieboldii var. seoulensis, and Zhong-yao-da-ci-dian(中葯大辭典) reported Asarum heterotropoides var. mandshuricum and A. sieboldii of Aristolochiaceae. In Korea, this crude drug has been used as a Korean folk remedy for tothache and aromatic. The botanical origin of 'Se Sin' is considered to be Asarum species of Aristolochoaceae. But there has no pharmacognostical confirmation on it. To clarify the botanical origin of 'Se Sin', we studied on the anatomical characteristics of Asarum species growing wildly in Korea i.e. A. maculatum, A. sieboldii, A. sieboldii var. seoulensis, and of 'Se Sin' from Korea on Korean market. Through our studies, the botanical origin of 'Se Sin' from Korea was proved to be whole plant of Asarum sieboldii and A. sieboldii var. seoulensis.

• Korean Journal of Pharmacognosy
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• v.15 no.4
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• pp.173-175
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• 1984

Fractionation of methanolic extract of Asarum sieboldii monitoring by bioassay resulted in the isolation of methyleugenol as a spasmolytic principle. Its $pD'_2$ value was 4.3.

• Korean Journal of Pharmacognosy
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• v.44 no.4
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• pp.350-361
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• 2013

Asarum sieboldii has been used for treatment of fever, pain, common cold, and chronic sinusitis in Korea. In this study, we performed quantification analysis of seven major constituents including aristolochic acid I, aristolochic acid II, ${\alpha}$-asarone, ${\beta}$-asarone, elemicin, methyl eugenol, and safrole in the 70% ethanol extract of Asarum sieboldii and its solvent fractions, n-hexane, ethylacetate, n-butanol, and water ones using a ultra-performance liquid chromatography-electrospray ionization-mass spectrometer(UPLC-ESI-MS) and gas chromatography-mass spectrometer(GC-MS). Regression equations of seven components were acquired with $r^2$ values >0.99. The values of limit of detection(LOD) and quantification(LOQ) were 0.1-3.9 ng/mL and 0.3-11.7 mg/mL, respectively. The amount of the seven compounds in Asarum sieboldii were not detected -143.66 mg/g. The established LC-MS/MS and GC-MS methods will be helpful to improve quality control of Asarum sieboldii.

• Korean Journal of Plant Taxonomy
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• v.40 no.4
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• pp.255-261
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• 2010

A new species, Asarum yeonbyeonense M. Kim & S. So, is named and described from Mt. Baekdu in Korea. This new species is distinctly different from the related species A. mandshuricum (Maxim.) M. Kim & S. So according to the purple band at the base of the calyx lobes and the thick calyx lobes. In addition, Asarum sieboldii var. viridiluteolum Y. Lee is closely related to A. yeonbyeonense M. Kim & S. So due to its reflexed calyx lobes; however, this variety has green calyx lobes and pubescent petioles. Therefore its scientific name was changed to Asarum yeonbyeonense var. viridiluteolum (Y. Lee) M. Kim & S. So, and its Korean name should be changed into Nokyeonbyeon-Jokdoripul.

• Journal of Life Science
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• v.20 no.11
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• pp.1697-1703
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• 2010

Asarum consists of low-growing herbs and is a genus in the Aristolochiaceae family with species found in the north temperate zones with most species in Asia. We evaluated the nine taxa with the trnL - trnT region of the chloroplast genome to estimate phenotypic relationships within genus Asarum in Korea. Alignment of the DNA sequences required the addition of numerous gaps. Sequence variation within the Asarum was mostly due to nucleotide inserts/deletions, although several indels and inserts were found. Another source of sequence divergence was length variation due to stretches of short repeats that occur at the trnL - trnT region in all the Asarum. A + T content for nine Korean species of genus Asarum ranged between 74.7% and 78.3%. These values were higher than those for the angiosperm alignments of the total trnL and trnT region (64.5~67.1%). Within genus Asarum, A. patens was strikingly different from the others in the three phylogenetic analyses (MP, ML, and NJ). However, some internal nodes were poorly supported. Within Korean Asarum, four species were unsolved portions. Possible reasons for the striking non-congruence between the previous morphological traits and the trnL - trnT based on phylogeny were discussed.

• Korean Journal of Plant Taxonomy
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• v.38 no.3
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• pp.251-270
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• 2008

Members of the genus Asarum of Korea were reviewed taxonomically, and classified into seven species. Among them, four species - A. koreanum, A. maculatum, A. patens and A. versicolor - were recorded as Korean endemics. The most valuable taxonomic characters in identifying the Asarum taxa were shape, size and color of the calyx tube and lobe. In addition, the presence of white spots on the adaxial surface, size and color of leaf, presence of trichome, shape and size of the stylar protuberance and thickness of the calyx lobe were also important characters. Keys to the species including descriptions and figures of floral structures of each taxon were provided here for the accurate identification of each species.

• Korean Journal of Plant Taxonomy
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• v.38 no.2
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• pp.121-149
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• 2008

Taxonomic treatment and the identification key for 6 species and 4 varieties from the genus Asarum (Aristolochiaceae) in Korea were presented on the basis of the morphological analyses. Recently the taxonomy of the genus Asarum in Korea is controversial in the definition of species and the establishment of variation range. Our morphological studies supported that the species A. patens, A. misandrum and A. versicolor should be recognized as independent species by the unique morphological characters such as calyx lobes, stylar protuberance and leaf variegation. Second, A. sieboldii var. cornutum, A. koreanum, A. maculatum and A. sonunsanense, regarded as species or variety by different scholars, showed a close relationship with A. sieboldii by the similar calyx characters. Thus, new combinations, such as A. sieboldii for. cornutum, A. sieboldii for. koreanum, A. sieboldii for. maculatum and A. sieboldii for. sonunsanense, are proposed. And A. heterotropoides var. seoulense and A. heterotropoides var. mandshuricum also had a close relationship, thus, new combinations, A. mandshuricum for. seoulense, A. mandshuricum for. mandshuricum, are proposed. Furthermore, it is appropriate that A. heterotropoides var. heterotropoides, only distributed in Japan, is revised into A. heterotropoides as independent species by the unique character from the 2 varieties above. Consequently, the genus Asarum in Korea is classified into 3 species and 7 forma.

• Korean Journal of Plant Resources
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• v.11 no.2
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• pp.131-135
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• 1998

Cathamus tinctorius, Coptis japonica and Asarum sieboldii were tested as substrate for the production of Flammulina velutipes. Among the C. tinctoris , C. japonica and A. sieboldii , C. tinctoris was the best substrate for the production of fruitbody. The effects of addition of C. tinctoris to sawdust substrate resulted in the increased mycelial growth on inoculum culture, 3.1% in ratio of fully culture and shorted one day in culture period. C. tinctoris was decreased 6.1% in ratio of fully culture, 11.0% in ratio of fruitbody productive culm. The addition of C.tinctoris, C.japonica to sawdust substrate increased 134.6%, 114.1% on the yield of the mushroom fruitbody respectively . But A. sieboldii decreased the mycelial growth and pineheading ratio delayed the production of fruitbody.

• Natural Product Sciences
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• v.25 no.1
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• pp.72-75
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• 2019

Bioassay-guided fractionation of the roots of Asarum sieboldii led to the isolation of the six compounds methylkakuol (1), sesamin (2), asarinin (3), xanthoxylol (4), and (2E,4E,8Z,10E/Z)-N-(2-methylpropyl) dodeca-2,4,8,10-tetraenamide (5/6). Among the isolates, xanthoxylol (4) exhibited significant cytotoxicity against human breast cancer cells MCF-7 and MDA-MB-231 in vitro with $IC_{50}$ values of 9.15 and $13.95{\mu}M$, respectively.

• Food Science of Animal Resources
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• v.28 no.4
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• pp.512-516
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• 2008

To search for anit-H5N1 influenza virus agent, the anti-viral activity of methanol and aqueous extracts from thirty medicinal plants were examined in this study. The plant material (30 g) was extracted with methanol (300 mL) for 24 hr at room temperature. Methanol extracts were filtered and evaporated, then freeze-dried. Aqueous extracts were prepared with dried plant material (30 g) and hot distilled water (300 mL). After 3 hr, the aqueous extracts were filtered and evaporated, then lyophilized. Extracts prepared from different plants were tested the antiviral activity against influenza viruses [A/vietnam/1194/04 (H5N1)-NIBRG-14] using the hemagglutination (HA) assay. Among the test plants, Asarum sieboldii was found to be a potent inhibitor of H5N1 influenza virus in MDCK cell culture. Virus titers were 7 log, whereas with methanol extract of Asarum sieboldii for 48 hr titers were 3 log, indicating that methanol extract of Asarum sieboldii inhibited the H5N1 influenza viruses from the infected cells.