• 한국통신학회논문지
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• 제38B권9호
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• pp.744-750
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• 2013

본 논문에서는 메타물질 특성을 구현하기 위하여 고임피던스 표면을 갖는 인공자기도체(AMC) 구조를 제안하였다. 설계한 AMC 구조는 3.2GHz에 적용하는 것을 목표로 하였으며, 특성 분석을 위해 다중의 AMC 구조를 일정하게 배열한 반사판으로 제작 및 측정하였다. AMC 반사판의 표면에 형성되는 높은 임피던스로 인하여 반사 특성이 좋아지고, 간섭 및 시스템의 크기를 줄이며, 안테나의 성능을 증가시킨다. 제안된 구조는 설계된 AMC와 접지면을 잇는 via hole을 사용하지 않고, 유전체의 두께와 유전율, 구조의 특성을 이용하여 고임피던스를 구현하였다. 기존의 연구된 via hole이 없는 구조와 비교하여 대역폭이 약 150% 증가하였다. 또한, 금속(PEC) 반사판과 동일한 반사특성을 보이는 대신, 안테나와 반사판 간의 거리를 ${\lambda}/10$까지 줄일 수 있다. 실험을 통하여 안테나와의 거리가 약 10mm 지점에서 방사 특성이 3dB 증가한 것을 확인하였다. 설계된 반사판은 반사거리가 작아 휴대용 무선통신기기의 내부에 삽입이 가능하며, 안테나의 효율을 증가시키고, 후방 방사를 차폐함으로써 전자파인체흡수율을 94% 이상 획기적으로 감소시킬 수 있다.

• 환경생물
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• 제33권3호
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• pp.352-359
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• 2015

본 연구는 낙동강 본류에 서식하는 Pectinatella magnifica의 출현양상을 조사하기 위해 발생시기인 2014년 7월~11월 동안 기본 분포조사와 출현밀도가 높은 지역을 대상으로 집중조사를 실시하였다. 그 결과, 낙동강 본류 구간 내 Pectinatella magnifica의 부착기질은 인공적으로 형성된 기질에서 12.3%, 자연기질에서 87.7%로써 자연기질에서 매우 높게 확인되었으나 자연기질에 포함된 식물군락의 특정 종에 따른 선호하는 정도는 유의한 차이를 보이지 않았다. 반면 본 조사 결과에서는 고착대상으로 하는 기질의 분포 정도의 차이는 P. magnifica의 분포 차이에 영향을 미치고 있음을 보여주었다. 그러므로 태형동물이 선호하는 출현기질의 증가는 P. magnifica의 성장 및 분포에 영향을 미치는 요인 중 하나로써 작용될 것으로 사료된다.

• 대한기계학회논문집A
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• 제30권11호
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• pp.1425-1432
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• 2006

Coating layers on a coated sheet steel frequently affect distributions of strain rate of sheets and deteriorate the frictional characteristics between sheets and tools in sheet metal forming. Thus, it is important to identify the deformation behavior of these coatings to ensure the success of the sheet forming operation. In this study, the technique using nano-indentation test, FE-simulation and Artificial Neural Network(ANN) were proposed to determine the power law stress-strain behavior of coating layer and the power law behavior of extracted coating layers was examined using FE-simulation of drawing and nano-indentation process. Also, deep drawing test was performed to estimate the formability and frictional characteristic of coated sheet, which was calculated using the linear relationship between drawing force and blank holding force obtained from the deep drawing test. FE-simulations of the drawing process were respectively carried out for single-behavior FE-model having one stress-strain behavior and for layer-behavior FE-model which consist of coating and substrate separately. The results of simulations showed that layer-behavior model can predict drawing forces with more accuracy in comparison with single-behavior model. Also, mean friction coefficients used in FE-simulation signify the value that can occur maximum drawing force in a drawing test.

• 한국미생물·생명공학회지
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• 제37권2호
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• pp.105-109
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• 2009

단백질 인산화를 통한 세포내 신호전달기구 중 serine/threonine kinase에 속하는 Akt/PKB는 세포 생존과 사멸, 당대사 등을 조절하는 것으로 알려져 있다. 이러한 이유로, Akt/PKB 단백질은 천연물질들로부터 항암제를 탐색하기 위한 한 가지 target으로 사용되어 왔다. 본 연구에서는 Akt/PKB 단백질을 대량으로 생산하기 위하여 대장균의 단백질 발현 시스템을 이용하여 human Akt/PKB 단백질을 발현시켰다. 대장균에서 대량 발현된 Akt는 일반적인 조건에서는 inclusion body를 형성하였다. 배양온도 $27^{\circ}C$에서 0.01-0.09 mM IPTG로 발현 유도 시 발현된 human Akt/PKB 단백질 상당 부분이 가용화 되었다. 발현된 Akt kinase를 $Ni^{2+}$-NTA agarose column으로 정제하고, anti-Akt antibody를 이용하여 정제된 단백질이 Akt kinase 임을 확인하였다. 정제된 human Akt/PKB 단백질은 세포추출물에 존재하는 인산화 단백질을 이용하여 in vitro에서 인산화 되었으며, 인산화된 활성형 human Akt/PKB protein kinase는 human Akt/PKB protein kinase 특이 형광 peptide를 특이적으로 인산화하였다.

• KSBB Journal
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• 제19권4호
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• pp.295-300
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• 2004

해양유래미생물에서 gelatin 분해능이 우수한 균주 Vibrio vulnificus CYK 279H를 이용하여 최적 효소활성조건을 검토하였다. 온도와 초기 pH는 $25^{\circ}C$, 7.5에서 높은 것으로 나타났으며, 단당, 이당, 다당류를 첨가하여 탄소원의 영향에서는, 0.3% (w/v) galactose 에서, 유$.$무기 질소원으로는 0.6% (w/v) yeast extract와 4.0% (w/v) gelatin, 0.2% (w/v) (NH$_4$)$_2$SO$_4$에서 효소활성이 가장 높게 나타났다. 염 농도로는 2.0% (w/v) NaCl, 금속이온은 Fe$^{2+}$를 첨가하였을 때 효소활성이 증가되었다. 선정된 최적배양조건에서 Vibrio vulnificus CYK 279H를 배양한 결과, 18 h 배양시 73 Unit/1로 기본배지 20 Unit/1보다 활성이 3.7배 증가한 것으로 확인되었다.

• Progress in Superconductivity
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• 제11권1호
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• pp.62-66
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• 2009

Introduction of proper impurity into $YBa_2Cu_3O_{7-x}$ (YBCO) thin films is an effective way to enhance its flux-pinning properties. We investigate effect of $Y_2O_3$ nanoparticles on the critical current density $J_c$ of the YBCO thin films. The $Y_2O_3$ nanoparticles were created perpendicular to the film surface (parallel with the c-axis) either between YBCO and substrate or on top of YBCO, YBCO/$Y_2O_3$/LAO or $Y_2O_3$/YBCO/STO, by pulsed laser deposition. The deposition temperature of the YBCO films were varied ($780^{\circ}C$ and $800^{\circ}C$) to modify surface morphology of the YBCO films. Surface morphology characterization revealed that the lower deposition temperature of $780^{\circ}C$ created nano-sized holes on the YBCO film surface which may behave as intrinsic pinning centers, while the higher deposition temperature produced much denser and smoother surface. $J_c$ values of the YBCO films with $Y_2O_3$ particles were either remained nearly the same or decreased for the samples in which YBCO is grown at $780^{\circ}C$. On the other hand, $J_c$ values were enhanced for the samples in which YBCO is grown at higher temperature of $800^{\circ}C$. The difference in the effect of $Y_2O_3$ can be explained by the fact that the higher deposition temperature of $800^{\circ}C$ reduces intrinsic pinning centers and $J_c$ is enhanced by introduction of artificial pinning centers in the form of $Y_2O_3$ nanoparticles.

• 한국버섯학회지
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• 제12권3호
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• pp.216-219
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• 2014

목이버섯 봉지재배의 배지 선발은 톱밥85%를 참나무 80%+포플러20%의 중량비로 혼합하였고 이 때 필요한 부재료는 면실박10%와 밀기울5%를 혼합한 배지였다. 개발한 배지자실체 수량검정을 한 결과 버섯생육기간은 대조에 비해 33일 단축되고 수량도 25%증수되었다.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.9-18
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• 2017

Nine bacilli with fibrinolytic activities were isolated from doenjang, a traditional Korean fermented soy food. Among them, RSB34 showed the strongest activity and was identified as Bacillus amyloliquefaciens by 16S rRNA and recA gene sequencing. During growth on LB up to 96 h, RSB34 showed the highest fibrinolytic activity ($83.23mU/{\mu}l$) at 48 h. Three bands of 23, 27, and 42 kDa in size were observed when the culture supernatant was analyzed by SDS-PAGE and 27 and 42 kDa bands by fibrin zymography. The gene encoding the 27 kDa fibrinolytic enzyme AprE34 was cloned by PCR. BLAST analyses confirmed that the gene was a homolog to genes encoding AprE-type proteases. aprE34 was overexpressed in Escherichia coli BL21(DE3) using pET26b(+). Recombinant AprE34 was purified and examined for its properties. The $K_m$ and $V_{max}$ values of recombinant AprE34 were $0.131{\pm}0.026mM$ and $16.551{\pm}0.316{\mu}M/l/min$, respectively, when measured using an artificial substrate, N-succinyl-ala-ala-pro-phe-p-nitroanilide. aprE34 was overexpressed in B. subtilis WB600 using pHY300PLK. B. subtilis transformants harboring pHYRSB34 (pHY300PLK with aprE34) showed higher fibrinolytic activity than B. amyloliquefaciens RSB34.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1125-1129
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• 2005

Cloning of a 6.6-kb BamHI digested chromosomal DNA from S. griseus IFO13350 revealed the presence of an additional gene encoding a novel trypsin-like enzyme, named SprU. The SprU protein shows a high homology ($79\%$ identity, $88\%$ similarity) with the SGT protease, which has been reported as a bacterial trypsin in the same strain. The amino acid sequence deduced from the nucleotide sequence of the sprU gene suggests that SprU is produced as a precursor consisting of an amino-terminal presequence (29 amino acid residues), prosequence (4 residues), and mature trypsin consisting of 222 amino acids with a molecular weight of 22.94 kDa and a calculated pI of 4.13. The serine, histidine, and aspartic acid residues composing the catalytic triad of typical serine proteases are also well conserved. When the trypsin activity of the SprU was spectrophotometrically measured by the enzymatic hydrolysis of the artificial chromogenic substrate, N-${alpha}$-benzoyl-DL-arginine-p-nitroanilide, the S. lividans transformant with pWHM3-U gave 3 times higher activity than that of control. When the same recombinant plasmid was introduced into S. griseus, however, the gene dosage effect was not so significant, as in the cases of other genes encoding serine proteases, such as sprA, sprB, and sprD. Although two trypsins, SprU and SGT, have a high degree of homology, the pI values, the gene dosage effect in S. griseus, and the gene arrangement adjacent to the two genes are very different, suggesting that the biochemical and biological function of the SprU might be quite different from that of the SGT.

• 보존과학회지
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• 제34권4호
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• pp.245-257
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• 2018

To investigate the deterioration of textiles with metal thread, I surveyed 40 textile objects, dating from the 11th to the 19th century from Korea, China, Japan, and Central Asia at the Metropolitan Museum of Art, New York. This survey included various types, widths, and thicknesses of metal thread. In addition, deterioration was examined under the microscope and color information was collected using the spectrophotometer. While investigating metal thread in the collections, I fabricated 12 different types of metal samples with metal leaf(24K gold, silver, and copper leaf), adhesive (hide animal glue and a mixture of hide animal glue and iron oxide red), and paper substrate(Korean mulberry and Taiwanese kozo paper). The accelerated deterioration process of those fabricated samples was carried out using a light box(UV and daylight), and a humidity cabinet. In the light experiment with blue scales textile fading card(aka, blue wool standard), the metal leaf began to peel off during the deterioration process with 756,000 lux-hours UV and daylight. In the temperature and humidity experiment, I could observe the reddish tarnish on copper, and some part of it began to peel off. Color reading on the light exposed samples showed that the degree of color change on the surface follows the amount of exposure as it increased over time. On the other hand, color change on the samples after artificial deterioration using temperature and humidity factors showed random change of color with occasional spikes. Distortion of original shape worsened in the samples exposed to temperature and humidity.