• Asian-Australasian Journal of Animal Sciences
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• v.24 no.10
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• pp.1348-1357
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• 2011

The objective of this study was to compare the effect of Butylated Hydroxy Toluene (BHT), Pentoxifylline (PTX) and ${\alpha}$-tocopherol (Vit E) on semen quality parameters of Karan Fries bulls. The fortification of extender by various semen additives improves motility as well as fertility of spermatozoa. Split samples of 24 ejaculates of four Karan Fries bulls were extended in extender with or without various additives such as BHT, PTX and Vit E, and performance was evaluated at an interval of 0, 24, 48 and 72 h at refrigerated temperature (4-$7^{\circ}C$). Results of the present study revealed that addition of BHT, PTX and Vit E in extender improved sperm cell function, such as motility, viability, HOST, and acrosome integrity, as compared to the control during liquid storage up to 48 h of preservation at refrigerated temperature. There was no significant (p<0.05) difference between any of the additives up to 48 h of preservation. Overall, the results showed a significant (p<0.05) deterioration in motility after each storage interval. The results showed a significant deterioration in the acrosome integrity and plasma membrane integrity up to 48 h; subsequently, there was not much degradation of both the semen quality parameters. There was a significant increase in spermatozoal tail and total abnormality after each storage interval at refrigerator temperature (4 to $7^{\circ}C$); however, the head and mid-piece abnormalities were almost unaffected. Tail and total abnormality were least in extender fortified with BHT, PTX and Vit E at different hours of incubation as compared to the control. The addition of 1.5 mM BHT, 3.6 mM PTX and 1 mg/ml Vit E in the semen extender has more beneficial effect in terms of semen quality and preservability of spermatozoa.

• International Journal of Industrial Entomology and Biomaterials
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• v.26 no.1
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• pp.61-66
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• 2013

Baculum elongatum has several peculiarities such as parthenogenesis, unique external features, changing body color, and dropping oviposition, which makes it a potential economically useful insect. Oviposition was performed by females without fertilization by the sperm of males. The oviposition period was $42.2{\pm}22.7$ days and the number of eggs per female was $109.5{\pm}70.5$ eggs. The hatch rate was 73.3% at $25^{\circ}C$ and 66.7% after low temperature treatment ($8^{\circ}C$ for 60 days). In nature, B. elongatum overwinters as an egg, but it can be assumed from the results that cold temperatures were not required for hatching. The hatch rate was 98.2% in the treatment using floral foam, fermented sawdust, and leaves. The developmental period was $100.9{\pm}4.2$ days for eggs, $55.3{\pm}4.6$ days for nymphs, and $49.7{\pm}16.0$ days for adults. The length of the eggs was $0.33{\pm}0.0$ cm and the lengths of the nymphs were clearly distinguishable according to the instar stage. Clover(Trifolium repens) was an excellent diet as it was similar to the host plant and could be used as an alternative diet. The rate of reaching adulthood for the insects was 66.7% on an artificial diet containing 25% acacia leaves. For sustainable mass rearing of nymphs or adults of B. elongatum, a natural diet could be used such as acacia (Robinia pseudoacacia), white oak (Quercus aliena), chestnut (Castanea crenata var. dulcis), and bush clover (Lespedeza bicolor), or an alternative diet such as clover or artificial diet.

• Korean journal of applied entomology
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• v.32 no.3
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• pp.291-299
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• 1993

Midgut tissues from 4 insect specIes were exammed for the activity of cytochrome P-450 monooxygenases, a major enzyme involved in chemical detoxification. When Helicoverpa assulta larvae were reared on an artificial d;et, the specific activity of the midgut cytochrome P-450 monooxygenases (MFO) was :3 times higher than that of the fat body, The specific activity of the midgut cytochrome P-450 monooxygenases was higher in H. assul/a larvae when reared on Nicotiana tabacum leaves than when on CapsIcum annuum fruits or an artificial diet. In the case of Hyphantria cunea larvae, Tilia megaphyllo leaves were the best in inducing midgut cytochrome P-450 monooxygenases activity. When larvae of H. assulta, Spodoptera exigua, H. cunea and Spodoptera litura were reared on their own artificial diet, the highest activity was seen in S. exigua larvae which is a polyphagous and insecticide-resistant strain.

• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.389-393
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• 2009

Regional distribution of mistletoe (Viscum album var.coloratum) and its habitual environments were investigated in order to obtain the basal data on the artificial propagation to cope with its increasing consume for medicine. Mistletoes inhabited throughout the overall region of the South Korea investigated. They were parasitic mainly to the Quercus spp. including Q. serrata and rarely to the Castanea crenata var. dulcis, Prunus serrulata var. spontanea, Alnus japonica, and Pyrus pyrifolia, etc. Mistletoes were not observed on the conifers such as Pinus densiflora and Pinus koraiensis and some deciduous broad-leaved trees species such as Zelkova serrata, Diospyros kaki, Acer mono, Acer palmatum, and Morus alba. Their habitats were located from zero to 1,200 m above sea level nevertheless the direction or slope of the mountains, suggesting that artificial propagation can be carried out nation widely to the well-grown parasite tree species. Parasitic specificity related to the physical and chemical characteristic of the epidermal tissues will be studied further.

• Korean journal of applied entomology
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• v.40 no.1
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• pp.31-40
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• 2001

Host plants or Ectinohoplia rufipes (Motschulsky) (En) were investigated in golf courses and laboratory. E. rufipes adults fed on 61 plant species of 27 families out of 101 plant species of 41 families supplied in laboratory. In addition, twenty-seven plant species in 8 families were observed to be fed on from golf courses. Thus, total number of host plants of E. rufipes were 84 species in 29 families. E. rufipes adults preferred Japanese green alder (Alnus firma) , cherry apple (Malus sieholdii), cherry (Prunus serrulata var. spontanea) and sweet oliver (Osmantus asiaticus) in golf courses . The preference of E. rufipes adults with A. firma , P. serrulata vu. spontanea and O. asiaticus was bioassayed in laboratory. E. rufies adults preferred A. firma consuming 58.1% to O. asiaticus and p. serrulata vats. spontanea. The most number of E. rufipes adults was attracted to damaged leaf of A. firma by E. rufipes representing 45.3clo and followed by artificial damaged leaf representing 26.5% , untried feeding leaf representing 12.9% and undamaged leaf representing 4.0%. More E. rufipes adults were attracted to damaged leaf of A. firma by 67.5% than artificially damaged leaf by 30.5% or undamaged leaf treated with feces of E. rufipes by 2%.

• Journal of Korean Society of Forest Science
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• v.62 no.1
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• pp.76-81
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• 1983

Pedicularis spp. are the alternate hosts of the white pine blister rust fungi in the forests of Korea, and Ribes spp. were found as other alternate hosts by artificial inoculation test. New biotypes (races) with different host selectivity and pathogenicity of the causal fungi were speculated at various places in Korea. The aeciospores of the fungi were collected from two isolated places, Pyeongchang and Namwoen, and artificially inoculated on Pedicularis resupinata and six species of the genus Ribes. Among the tested alternate host species, P. resupinata and R. montigenum were only susceptible to both the Pyeongchang population and the Namwoen population, showing similar pathogencity between the two fungai populations. Three white pine species were planted in fungus-infested stands to study the susceptibility of each tree species. Four years later, 17.7% of Pinus koraiensis and 26.3% of the P. strobus were infected, but P. parviflora was free from attack. From the above results, it was concluded that the white pine blister rust fungus populations in Korea are of the same biotype, with P. koraiensis and P. strobus as aecial hosts, and Pedicularis spp. and Ribes spp. as telial hosts.

• Mycobiology
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• v.29 no.2
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• pp.107-109
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• 2001

Anthracnose symptoms severely occurred up to 50% on leaves of English ivy growing in greenhouses in Cheongwon area of Korea during disease survey in April, 2000. The symptoms developed as concentric spots with dot-like acervuli on leaves of the plant. A total of 24 isolates of Colletotrichum sp. were obtained from the spotted lesions and identified as Colletotrichum trichellum based on the morphological and cultural characteristics. Leaf spots similar to the original anthracnose symptoms were induced on the host leaves by artificial inoculation with the isolates of the fungus. This is the first record of C. trichellum causing anthracnose of English ivy in Korea.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.132.2-132
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• 2003

Anthracnose occurred on rapsberry grown in Gochang areas of Korea in 2003. The disease incidence was ranged from 1.1 to 2.6％. Anthracnose of rapsberry appeared as dark brown circular spots on naturally infected stems. The symptoms of infected stems were small brown to dark brown spots and gradually enlarged larger cylindrical dark brown lesions. The causal fungus of anthracnose isolated from the diseased plants was identifed as Colletotrichum coccodes based on the morphological and cultural characteristics. All isolates of C. coccodes were produced similar symptoms on the host leaves by artificial inocultion.

• The Plant Pathology Journal
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• v.18 no.1
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• pp.54-55
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• 2002

Soaked black rot symptom was observed on the stem of Angelica acutiloba from July to August 2000 at Kumsan, Chungnam in Korea. This disease usually occurred under humid and high temperature conditions. The lesions on the stem appeared as soft rot with brown elliptical spots, which developed into large black spots at a later stage. When the bacterial isolates from the diseased plants were inoculated onto healthy plants by artificial needle prick method, symptoms similar to that observed in the fields developed. According to the cultural characteristics and pathogenicity of the isolates on the host plant the causal bacterium was identified as Xanthomonas campestris. This study proposed that the disease be named "bacterial black stem rot of A. acutiloba"loba".

• The Plant Pathology Journal
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• v.15 no.1
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• pp.62-67
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• 1999

Anthracnose occurred severely on safflower plants grown in Euiseong and Jecheon areas of Korea in 1997 and 1998. The disease incidence was up to 100% in some fields, and symptoms developed on seedlings, leaves, stems, roots and hulls of the plants. Coletotrichum sp. was consistently present on the diseased plant parts, and all the isolates from the lesions were identified as Colletotrichum acutatum based on the morphological and cultural characteristics. Similar symptoms were produced on the host plants by artificial inoculation with isolates of the fungus. The fungus was reisolated from lesions on the plants inoculated. This is the first report that c. acutatum causes anthracnose of safflower.