• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.635-638
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• 2004

An ATP-binding-cassette (ABC) transporter gene in the cephabacin biosynthetic gene cluster of Lysobacter lactamgenus was characterized. The amplified orf10 (cpbJ) gene was subcloned into pET-28a(+) vector and expressed in E. coli BL21(DE3) strain by 0.5 mM IPTG at $30^{\circ}C$. The membrane fraction of recombinant E. coli cells was separated by ultracentrifugation, and solubilized using 2.5% octyl-$\beta$-D-glucoside. Using the solubilized membrane fraction, the artificial proteoliposomes were reconstituted and analyzed for the biological activity of CpbJ protein. Upon measuring ATPase activity, the proteoliposome made from recombinant E. coli membrane proteins showed slightly higher activity than that from host E. coli membrane proteins. In the measurement of membrane transport activity, the reconstituted proteoliposome of recombinant E. coli membrane proteins exhibited higher activity when both substrates of cephalosporin C and L-Ala-L-Ser were applied, compared to the case of cephalosporin C or L-Ala-L-Ser only. It implies that the CpbJ protein is an ABC transporter secreting cephabacin antibiotics synthesized in cytoplasm.

• 전자공학회논문지CI
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• 제38권5호
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• pp.37-43
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• 2001

인터넷이 널리 이용되면서 네트워크나 호스트에 대한 불법적인 침입은 많은 위험요소가 되고 있다. 이러한 침입을 탐지하기 위하여 통계적기법, 데이터마이닝기법, 유전자 알고리즘/프로그래밍 기법 등을 이용한 이상현상 탐지모델들이 많이 제안되어 왔으나 새로운 유형의 침입에 대해서는 탐지능력이 떨어지는 단점이 있다. 본 논문에서는 THRE KBANN을 이용한 이상현상탐지모델을 제안하였는데, 이는 연속학습을 할 수 있도록 지식기반신경망을 개선한 것이다. 이 모델을 실험적 자료에 적용한 결과를 데이터마이닝기법을 적용한 경우와 비교하여 성능평가를 하였다. 그리고 새로운 침입유형을 탐지하기 위한 연속학습에 대한 성능도 평가하였다.

• Parasites, Hosts and Diseases
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• 제56권4호
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• pp.313-324
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• 2018

This article reviews Leptotrombidium deliense, including its discovery and nomenclature, morphological features and identification, life cycle, ecology, relationship with diseases, chromosomes and artificial cultivation. The first record of L. deliense was early in 1922 by Walch. Under the genus Leptotrombidium, there are many sibling species similar to L. deliense, which makes it difficult to differentiate L. deliense from another sibling chigger mites, for example, L. rubellum. The life cycle of the mite (L. deliense) includes 7 stages: egg, deutovum (or prelarva), larva, nymphochrysalis, nymph, imagochrysalis and adult. The mite has a wide geographical distribution with low host specificity, and it often appears in different regions and habitats and on many species of hosts. As a vector species of chigger mite, L. deliense is of great importance in transmitting scrub typhus (tsutsugamushi disease) in many parts of the world, especially in tropical regions of Southeast Asia. The seasonal fluctuation of the mite population varies in different geographical regions. The mite has been successfully cultured in the laboratory, facilitating research on its chromosomes, biochemistry and molecular biology.

• 한국식물병리학회지
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• 제14권6호
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• pp.651-653
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• 1998

A destructive anthracnose of safflower has severely occurred at Kyeongsangnam-do Agricultural Research and Extention Services in 1998. Incidence of the disease surveyed at 12 fields in Chinju, Sanchong and Hamyang was ranged fro 55.7 to 85.4%. Small irregular yellowish to brown spots appeared on all parts of the plant at initial infection stage and the spots enlarged or united into larger irregular to cylindrical black lesions with hollows. Above portions of infected stems were often blighted and pink colored conidial masses were colonized on the lesions. The causal fungus consistently isolated from the infected tissues were identified as Colletotrichum acutatum by following characteristics. The fungus grew well on PDA at 15~3$0^{\circ}C$ developing apricot to salmon color. Conidia were single-celled, colorless, fusiform and bisectioned and measured as 8~18$\times$3~5 ${\mu}{\textrm}{m}$. Brownish appressoria were cylindrical or ovate and sized as 5~10$\times$4~8 ${\mu}{\textrm}{m}$. Optimum temperature for growth and germination of conidia was recorded at $25^{\circ}C$ and at least 6 hours were required for infection to the host plants. Over 90% conidia were germinated and all plants were infected by artificial inoculation by 24 hours at $25^{\circ}C$. This is the first report of safflower anthracnose caused by C. acutatum in Korea.

• 한국응용곤충학회지
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• 제28권2호
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• pp.82-87
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• 1989

담배거세미나방핵다각체병바이러스대량생산연구에서, 5영충에 $1.1{\;}{\times}{\;}10^7다각체/ml$로 접종 후 8일에 수확했을 때 한 마리당 $6.7{\;}\times{\;}10^9$ 다각체를생산할 수 있었으며, 이때 통과는 한 마리당 약 2g의 인공통과로 충분하였다. 합성유약 호르몬인 methoprene ($Manta^{\circledR}$)을 처리했을 때 종영유충기간이 1~2일 연장되었으며, 바이러스 생산에 있어서도 무처리에 비해 약 15% 증가되었다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2001년도 추계종합학술대회
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• pp.479-482
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• 2001

침입 탐지 시스템은 침입 판정과 감사 데이터(audit data) 수집 분야에서 많은 연구가 진행되고 있다. 침입 판정은 주어진 일련의 행위들이 침입인지 아닌지를 정확히 판정해야 하고 감사 자료 수집에서는 침입 판정에 필요한 데이터만을 정확히 수집하는 능력이 필요하다 최근에 이러한 문제점을 해결하기 위해 규칙기반 시스템과 신경망 등의 인공지능적인 방법들이 도입되고 있다. 그러나 이러한 방법들은 단일 호스트 구조로 되어있거나 변형된 침입 패턴이 발생했을 때 탐지하지 못하는 단점이 있다. 따라서, 본 논문에서는 분산된 이기종 간의 호스트에서 사용자의 행위를 추출하여 패턴을 검색, 예측할 수 있는 데이터 마이닝을 적용하여 실시간으로 침입을 탐지하는 방법을 제안하고자 한다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2002년도 춘계종합학술대회
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• pp.619-622
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• 2002

침입 탐지 시스템은 침입 판정과 감사 데이터(audit data) 수집 분야에서 많은 연구가 진행되고 있다. 침입 판정은 주어진 일련의 행위들이 침입인지 아닌지를 정확히 판정해야 하고 감사 자료 수집에서는 침입 판정에 필요한 데이터만을 정확히 수집하는 능력이 필요하다. 최근에 이러한 문제점을 해결하기 위해 규칙기반 시스템과 신경망 등의 인공지능적인 방법들이 도입되고 있다. 그러나 이러한 방법들은 단일 호스트 구조로 되어있거나 변형된 침입 패턴이 발생했을 때 탐지하지 못하는 단절이 있다. 따라서, 본 논문에서는 분산된 이기종 간의 호스트에서 사용자의 행위를 추출하여 패턴을 검색, 예측할 수 있는 데이터 마이닝 에이전트를 적용하여 실시간으로 침입을 탈지하는 방범을 제안하고자 한다.

• The Plant Pathology Journal
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• 제17권1호
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• pp.45-51
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• 2001

A root rot/vine decline disease occurred naturally on bottle gourd-stocked watermelon, melon, oriental melon and squash grown in greenhouses, but not on these plants grown in fields. Self-rooted watermelon, cucumber, pumpkin and luffa were also proven to be hosts of the pathogen by artificial inoculation in this experiment. The pathogen was identified as Monosporascus cannonballus by comparing microscopic characteristics of fungal structures with those of previously identified fungal strains. Our field investigations showed that the temperature and electric conductivity of soil in infected greenhouses were higher and the soil moisture content was lower than in noninfected greenhouses. To investigate soil-environmental factors affecting disease development, greenhouse trials and inoculation experiments were conducted. The host plants inoculated and grown under conditions of high soil temperature and electrical conductivity ($35\pm2^{\circ}$, 3.2-3.5 mS) and with low soil moisture content (pF 3.0-4.5) were most severely damaged by the fungal disease. Since plants growing in greenhouses ae usually exposed to such environmental conditions, this may be the reason why the monosporascus root rot/vine decline disease has occurred only on cucurbits cultivated in greenhouses but not in field conditions.

• 반도체디스플레이기술학회지
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• 제18권4호
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• pp.40-45
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• 2019

The smart factory has developed with the help of several technologies such as automation, artificial intelligence, big data, smart sensors and communication protocols. The Internet of things(IOT) among communication protocols has become the key factor for the seamless integration of various manufacturing equipment. Therefore, it is important that the IOT cooperate with the standards of communication protocols proposed by the SEMI in the semiconductor industry. In this paper, we suggest a novel reference model of the communication protocols for semiconductor equipment by introducing an IOT service layer. With the IOT service layer, we can use the functions and the additional services provided by the IOT standards that give the inter-operability between factory machines and host computers. We implement the standard of the communication protocols for semiconductor equipment with the IOT service layer by using ns3 simulator. It concludes that it is necessary to provide the platform for the IOT service layer to deploy efficiently the proposed reference model of the communication protocols.

• Journal of Microbiology and Biotechnology
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• 제32권2호
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• pp.205-211
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• 2022

Probiotics can effectively modulate host immune responses and prevent gastrointestinal diseases. The objective of this study was to investigate the probiotic characteristics of Lactobacillus brevis KU15152 isolated from kimchi and its protective potential against intestinal inflammation induced by Staphylococcus aureus lipoteichoic acid (aLTA). L. brevis KU15152 exhibited a high survival rate in artificial gastric and bile environments. Additionally, the adhesion capability of the strain to HT-29 cells was higher than that of L. rhamnosus GG. L. brevis KU15152 did not produce harmful enzymes, such as β-glucuronidase, indicating that it could be used as a potential probiotic. The anti-inflammatory potential of L. brevis KU15152 was determined in HT-29 cells. Treatment with L. brevis KU15152 suppressed the production of interleukin-8 without inducing significant cytotoxicity. The downregulatory effects of L. brevis KU15152 were involved in the suppression of nuclear factor-kappa B activation mediated by the extracellular signal-regulated kinase and Akt signaling pathways. Collectively, these data suggest that L. brevis KU15152 can be used in developing therapeutic and prophylactic products to manage and treat aLTA-induced intestinal damage.