• 제목/요약/키워드: Artificial host

검색결과 153건 처리시간 0.024초

네발나비과 나비류의 계대사육법 체계확립 (Establishment of the Successive Rearing System of Brush-footed Butterflies (Lepidoptera: Nymphalidae))

  • 설광열;김남정;홍성진
    • 한국응용곤충학회지
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    • 제44권4호
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    • pp.257-264
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    • 2005
  • 곤충관의 연중 전시용으로서 붉은 색 계통의 화려한 나비로서 공급하기 위하여 네발나비류의 계대사육법 체계확립을 위해 일련의 시험을 수행하였다. 먼저 실내 제대사육용 인공사료를 개발하기 위해 3종의 사료를 공시한 결과 종에 따라 선호하는 사료의 종류가 달랐으나 Bu사료가 대체로 사육 성적이 양호하였으며 사료 중 기주잎 분말은 냉동 건조한 것이 좋았으며 C/N율은 1:1이 가장 양호한 결과로 나타났다. 또한 발육단계 별 냉장가능기간을 조사한 결과 알의 경우 $5^{\circ}C$에서는 3일간, $15^{\circ}C$에서는 5일간 저장이 가능하였고 번데기의 경우에는 저온에 오래 보관할수록 기형 나비의 출현율이 높아 $15^{\circ}C$에서 최대 15일간 냉장 가능한 것으로 판단되었다. 나비의 장기 저온보호시험을 통해 냉장전의 흡밀기간, 냉장 온도에는 관계없이 법 장기간이 생존을, 산란전기간 및 산란수에 영향하였으며 60일까지 냉장이 가능한 것으로 나타났다. 네발나비는 기주식물의 자극없이는 산란하지 않아 페트리 접시를 이용한 간이 인공산란용 키트를 개발하여 기주식물(한삼덩굴)잎의 에테르 물추출물을 처리한 결과 암컷 1마리 당 평균 $278{\pm}27$개를 산란하였다. 이러한 결과들을 종합하여 네발나비류의 연중 실내제대사육 체계도를 완성하였다.

Ostrinia속(나비목: 포충나방과) 팥 해충의 종 동정과 발육 특성 (Species Identification and Developmental Biology of a Red Bean Pest in Ostrinia sp. (Lepidoptera: Crambidae))

  • 정진교;서보윤;박두상;오현우;이관석;박해철;조점래
    • 한국응용곤충학회지
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    • 제51권4호
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    • pp.469-477
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    • 2012
  • 본 연구에서는 팥의 꼬투리와 줄기를 가해하는 Ostrinia속 해충에 대해 종 동정 과정을 기술하였고, 사육하면서 관찰된 발육특성들을 보고하였다. 수컷의 생식기는 3-lobed uncus 형태였으며, 가운뎃다리 종아리마디에는 털을 많이 갖는 것으로 나타났다. 미토콘드리아 cytochrome oxidase I (COI)과 II (COII) 유전자의 부분 염기서열은 콩줄기명나방(O. scapulalis), 큰섬들명나방(O. zaguliaevi), 큰조명나방(O. zealis bipatrialis)들에 대해 일본과 중국에서 보고된 서열들과 100% 일치를 보이는 종은 없었다. 기주식물 범위는 국내외 보고들 간에 일치하지 않았다. 암컷 성페로몬샘 추출물의 가스크로마토그래피 분석에서 큰섬들명나방과 큰조명나방의 성페로몬 성분인 (Z)-9-tetradecenyl acetate는 검출되지 않았다. 이상의 결과들을 종합하여 고려하였을 때, 본 연구의 팥 해충을 가해하는 곤충 종은 콩줄기명나방(O. scapulalis)일 것으로 추정되었다. 가을 야외에서 채집된 유충들을 야외조건에서 보관하였을 때, 이듬해 6월과 7월 사이에 성충들이 우화하였는데, 이 결과로부터 본 곤충 종은 말령 유충 단계에서 겨울휴면을 하는 것으로 추정되었다. 이외에 반합성 인공사료를 이용하여 실내 사육이 가능하였다.

Root Rot of Moth Orchid Caused by Fusarium spp.

  • Kim, Wan-Gyu;Lee, Byung-Dae;Kim, Woo-Sik;Cho, Weon-Dae
    • The Plant Pathology Journal
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    • 제18권4호
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    • pp.225-227
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    • 2002
  • Moth orchid plants with yellowing blight and root rot symptoms were collected, and a total of 54 isolates of Fusarium spp. was obtained from roots and leaf bases of the diseased plants. The isolates were identified based on their morphological characteristics. Out of the 54 isolates of Fusarium spp., 42 isolates were identified as F. solani, 5 isolates as F. oxysporum, and 7 as F. proliferatum. Isolates of the three Fusarium spp. were tested for pathogenicity to moth orchid plants by artificial inoculation. All the Fusarium spp. induced root rot of the host plants. The symptoms progressed up to the basal part of the leaves, which later caused yellowing blight. The symptoms induced on the plants by artificial inoculation with the Fusarium spp. isolates were similar to those observed in greenhouses. The present study reveals that F. oxysporum, F. proliferatum, and F. solani cause root rot of moth orchid, and that F. solani is the main pathogen of the disease.

닭의 정자선(精子腺) 기능향상(機能向上)을 위한 연구(硏究) 3. 자궁질(子宮窒) 접합부선(接合部腺)과 난관(卵管) 누두부선(漏斗部腺)의 정자보유(精子保有) 차이에 대하여 (Studies on functional elevations of sperm-host glands in domestic hens 3. Comparison of stored-spermatozoa levels in the utero-vaginal glands and the infundibular glands)

  • 곽수동
    • 대한수의학회지
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    • 제31권2호
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    • pp.131-135
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    • 1991
  • The purpose of this study was designed to compare the storage stage of spermatozoa in the utero-vaginal (U-V) glands and the infundibular glands of high-fecundity hens. These laying hens were assigned to four groups by date intervals after last artificial inseminations. The U-V glands and the infundibular glands in the tissue preparation of the each hen uterus were observed microscopically, and also the appearance rates of spermatozoa-contained U-V glands were compared with those of the infundibular glands. 1. The appearance rates of spermatozoa-contained U-V glands were found to be 27.8, 28.8, 19.9 and 11.1% respectively at the hen groups of 1, 3, 7 and 10 days after artificial insemination. 2. The appearance rates of spermatozoa-contained infundibular glands were found to be 0.5, 1. 1, 0.6 and 0.4% of 1, 3, 7 and 10 days after AI and number of spermatozoa contained in a infundibular glands tended predominantly to be 1 to 2. So this study concluded as follows: The appearance rates of spermatozoa-contained glands were found to be higher in the U-V glands than in the infundibular glands and also spermatozoa number per gland were more numerous in U-V glands than in infundibular glands.

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웹 환경에서 인공신경망을 이용한 증상 진단 시스템 (Symptoms - Diagnostic System using Artificial Neural Networks in a Web Environment)

  • 김삼근;김병천
    • 정보처리학회논문지B
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    • 제9B권4호
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    • pp.407-414
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    • 2002
  • 최근 자신의 건강에 관한 관심이 고조됨에 따라 웹 상에서 많은 증상 진단 사이트들이 대두되고 있다. 그러나 기존의 건강정보 사이트들은 사용자에게 매우 제한된 기능만을 제공하고 있다. 본 논문에서는 신경망의 학습 효과를(전문가의 지식이 아니라) 진단 과정에 통합되도록 함으로써 유연한 증상-진단 도구를 제안한다. 즉 사용자(흑은 전문가)가 웹 상에서 단계별로 지정한 증상들을 바탕으로 하여 신경망 모델에 적용함으로써 보다 유연하게 사용자의 질병을 예측할 수 있는 새로운 알고리즘을 개발한다. 제안한 알고리즘은 두 가지 중요한 특징을 가진다 : 1) 일반 사용자들은 조기에 자신의 질병에 대한 진단을 받을 수 있고, 2) 전문가는 예상 질병 목록과 함께 각 질병의 가능성(확률)을 참조함으로써 진단의 정확성을 높일 수 있다는 점이다.

Non-contact monitoring of 3-dimensional vibrations of bodies using a neural network

  • Ha, Sung Chul;Cho, Gyeong Rae;Doh, Deog-Hee
    • Journal of Advanced Marine Engineering and Technology
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    • 제39권10호
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    • pp.1011-1016
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    • 2015
  • Gas piping systems in power plants and factories are always influenced by the mechanical vibrations of rotational machines such as pumps, blowers, and compressors. Unusual vibrations in a gas piping system influence possible leakages of liquids or gases, which can lead to large explosive accidents. Real-time measurements of unusual vibrations in piping systems in situ prohibit them from being possible leakages owing to the repeated fatigue of vibrations. In this paper, a non-contact 3-dimensional measurement system that can detect the vibrations of a solid body and monitor its vibrational modes is introduced. To detect the displacements of a body, a stereoscopic camera system is used, through which the major vibration types of solid bodies (such as X-axis-major, Y-axis-major, and Z-axis-major vibrations) can be monitored. In order to judge the vibration types, an artificial neural network is used. The measurement system consists of a host computer, stereoscopic camera system (two-camera system, high-speed high-resolution camera), and a measurement target. Through practical application on a flat plate, the measured data from the non-contact measurement system showed good agreement with those from the original vibration mode produced by an accelerator.

Occurrence of Dry Rot on Cymbidium Orchids Caused by Fusarium spry. in Korea

  • Kim, Wan-Gyu;Lee, Byung-Dae;Cho, Weong-Dae;Sung, Jae-Mo
    • The Plant Pathology Journal
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    • 제18권3호
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    • pp.156-160
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    • 2002
  • Cymbidium orchids with blight and rot symptoms were collected, and a total of 63 isolates of Fusarium app. was obtained from pseudobulbs, roots, and leaves of the diseased plants. The isolates were identified based on their morphological characteristics. Out of the 63 isolates of Fusatium sup., 51 isolates were identified as F. oxysporum, 10 isolates as F. solani, and the rest as F. proliferatum. F. oxysporum was isolated from all the Cymbidium spp., while F. solani and F. proliferatum were isolated only from Cymbidium ensifolium and C. ginatum, respectively. Isolates of the three Fusarium spp. were tested for pathogenicity to their hosts by artificial inoculation. The strongly pathogenic isolates of Fusarium spp. induced severe dry rot of pseudobulbs and roots of the host plants. The symptoms progressed up to the basal part of the leaves, which later caused blight of the entire plant. The dry root symptoms induced on the plants by artificial inoculation with the isolates of Fusarium app. were similar to those observed in the growers'greenhouses. This is the first report of dry rot of Cymbidium spp. caused by F. oxysporum, F. solani, and F. proliferatum in Korea.

Foot Rot of Bok Choy and Kale Caused by Rhizoctonia solani AG-2-1 in Korea

  • Kim, Wan-Gyu;Lee, Gyo-Bin;Shim, Hong-Sik;Cho, Weon-Dae
    • 한국균학회지
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    • 제49권1호
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    • pp.133-137
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    • 2021
  • Foot rot symptoms were occasionally observed on young bok choy (Brassica rapa subsp. chinensis) and kale (B. oleracea var. viridis) plants grown in vinyl greenhouses located in Icheon and Yangpyeong, Gyeonggi Province, Korea. These observations were made during disease surveys in April 2020. The incidence of diseased plants in the vinyl greenhouses investigated was 0.5-1.0% in bok choy and 0.5-5.0% in kale. Five isolates of Rhizoctonia sp. were obtained from diseased roots of bok choy and three isolates of Rhizoctonia sp. were taken from diseased stems of kale. All the Rhizoctonia sp. isolates were identified as Rhizoctonia solani AG-2-1 based on the morphological characteristics and anastomosis test. Three isolates each of R. solani AG-2-1 from bok choy and kale were tested for pathogenicity in their host plants by artificial inoculation. The tested isolates induced foot rot symptoms on the inoculated bok choy and kale plants. The symptoms on the bok choy and kale, induced by the artificial inoculation, were similar to those observed on plants from the vinyl greenhouses that were investigated. This is the first report of R. solani AG-2-1 causing foot rot in bok choy and kale in Korea.

Artificial Intelligence Plant Doctor: Plant Disease Diagnosis Using GPT4-vision

  • Yoeguang Hue;Jea Hyeoung Kim;Gang Lee;Byungheon Choi;Hyun Sim;Jongbum Jeon;Mun-Il Ahn;Yong Kyu Han;Ki-Tae Kim
    • 식물병연구
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    • 제30권1호
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    • pp.99-102
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    • 2024
  • Integrated pest management is essential for controlling plant diseases that reduce crop yields. Rapid diagnosis is crucial for effective management in the event of an outbreak to identify the cause and minimize damage. Diagnosis methods range from indirect visual observation, which can be subjective and inaccurate, to machine learning and deep learning predictions that may suffer from biased data. Direct molecular-based methods, while accurate, are complex and time-consuming. However, the development of large multimodal models, like GPT-4, combines image recognition with natural language processing for more accurate diagnostic information. This study introduces GPT-4-based system for diagnosing plant diseases utilizing a detailed knowledge base with 1,420 host plants, 2,462 pathogens, and 37,467 pesticide instances from the official plant disease and pesticide registries of Korea. The AI plant doctor offers interactive advice on diagnosis, control methods, and pesticide use for diseases in Korea and is accessible at https://pdoc.scnu.ac.kr/.

Effect of Human or Mouse IL-7 on the Homeostasis of Porcine T Lymphocytes

  • Ji Hwa Hong;Sang Hoon Kim;Hyun Gyung Kim;Jun Ho Jang;Ryeo Gang Son;Seung Pil Pack;Young-Ho Park;Philyong Kang;Kang-Jin Jeong;Ji-Su Kim;Hanbyeul Choi;Sun-Uk Kim;Yong Woo Jung
    • IMMUNE NETWORK
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    • 제21권3호
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    • pp.24.1-24.13
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    • 2021
  • Due to the inconsistent fluctuation of blood supply for transfusion, much attention has been paid to the development of artificial blood using other animals. Although mini-pigs are candidate animals, contamination of mini-pig T cells in artificial blood may cause a major safety concern. Therefore, it is important to analyze the cross-reactivity of IL-7, the major survival factor for T lymphocytes, between human, mouse, and mini-pig. Thus, we compared the protein sequences of IL-7 and found that porcine IL-7 was evolutionarily different from human IL-7. We also observed that when porcine T cells were cultured with either human or mouse IL-7, these cells did not increase the survival or proliferation compared to negative controls. These results suggest that porcine T cells do not recognize human or mouse IL-7 as their survival factor.