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Effect of Human or Mouse IL-7 on the Homeostasis of Porcine T Lymphocytes

  • Ji Hwa Hong (Departments of Pharmacy, Korea University) ;
  • Sang Hoon Kim (Departments of Pharmacy, Korea University) ;
  • Hyun Gyung Kim (Departments of Pharmacy, Korea University) ;
  • Jun Ho Jang (Departments of Pharmacy, Korea University) ;
  • Ryeo Gang Son (Department of Biotechnology and Bioinformatics, Korea University) ;
  • Seung Pil Pack (Department of Biotechnology and Bioinformatics, Korea University) ;
  • Young-Ho Park (Futuristic Animal Resource & Research Center (FARRC)) ;
  • Philyong Kang (Futuristic Animal Resource & Research Center (FARRC)) ;
  • Kang-Jin Jeong (Futuristic Animal Resource & Research Center (FARRC)) ;
  • Ji-Su Kim (National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Hanbyeul Choi (Departments of Pharmacy, Korea University) ;
  • Sun-Uk Kim (Futuristic Animal Resource & Research Center (FARRC)) ;
  • Yong Woo Jung (Departments of Pharmacy, Korea University)
  • Received : 2021.04.22
  • Accepted : 2021.06.20
  • Published : 2021.06.30
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Abstract

Due to the inconsistent fluctuation of blood supply for transfusion, much attention has been paid to the development of artificial blood using other animals. Although mini-pigs are candidate animals, contamination of mini-pig T cells in artificial blood may cause a major safety concern. Therefore, it is important to analyze the cross-reactivity of IL-7, the major survival factor for T lymphocytes, between human, mouse, and mini-pig. Thus, we compared the protein sequences of IL-7 and found that porcine IL-7 was evolutionarily different from human IL-7. We also observed that when porcine T cells were cultured with either human or mouse IL-7, these cells did not increase the survival or proliferation compared to negative controls. These results suggest that porcine T cells do not recognize human or mouse IL-7 as their survival factor.

Keywords

Acknowledgement

We thank Kyong Hoon Kim, Seung-min Yeon and Aryeong Choi for their critical comments. This study was supported by grant from the KRIBB Research Initiative Program (KGM4252122) and by Basic Science Research Program (grant NRF-2020R1F1A1048531, NRF-2019R1A6A1A03031807, and NRF-2021R1A2C2004279) of the National Research Foundation of Korea.

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