• 제목/요약/키워드: Artificial host

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인공숙주에서 증식된 Tetrastichus sp.의 생물학적 특성 (Biological Characteristics of Tetrastichus sp. reared on Artificial Host)

  • 이장훈;이기상;이해풍
    • 한국응용곤충학회지
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    • 제41권2호
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    • pp.99-105
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    • 2002
  • Tetrastichus sp.는 미국흰불나방의 번데기 기생벌로서, 1개의 숙주에 여러마리의 자손을 번식하는 집단 기생벌이며, 일년에 여러 세대 발생한다. 작잠, 신생아용 분유, 난황, 로얄젤리 등을 주요성분으로 기생벌의 인공먹이를 조성하였으며, polypropylene필름으로 번데기형태를 만들어 인공숙주로 사용하였다. 인공숙주에서 발육한 기생벌은 자연숙주에서 자란 기생벌보다는 크기가 작았으나, 계대 사육으로 번식 가능한 자손을 생산하였다. 인공숙주에서 발육한 in vitro 2세대의 생물학적 특성은 in vitro 1세대보다 훨씬 개선 었다. 평균 자손 수, 산란기간, 수명 등은 in vitro 에서 45.7,7.8, 13.8일 이었고 암컷 편중의 성비 76.9%를 나타내었다. 이러한 결과는 Tetrastichus sp.가 in vitro 대량사육에 적합한 기생벌임을 보여준다.

겨우살이의 종자의 기주목 접종 및 유묘 활착기술 (Germination and Seedling Induction of Viscum album var. coloratum (Kom.) Ohwi after Artificial Inoculation on the Branch of Host Plants)

  • 김철우;이재선
    • Journal of Forest and Environmental Science
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    • 제29권2호
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    • pp.173-180
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    • 2013
  • Berries of Korean mistletoe (Viscum album var. coloratum [Kom.] Ohwi) contained one seed, which have, in general, one or two embryos but very rarely three embryos. Mucilaginous substances in berries may help them adhere to the branches of host trees. It was observed that seeds need more than one and half years to develop into normal and healthy seedlings from the time of inoculation. Many factors such as adhesion of berry, thickness of host branch, orientation of haustorial root, etc. influenced the successful development of mistletoe plants. Through the application of six-year observation results on the germination of seeds and growth of seedlings, about 80% of germination rate for mistletoe seeds and 61% of survival ratio for germinated seeds, which is more than 23 times higher in natural conditions, were obtained after inoculation of seeds on the one-year-old branches of Malus pumila var. dulcissima and Quercus mongolica trees. The technological aspects of the success can be applied to other host plants and provide a critical clue to an artificial propagation system, for this medicinally valuable genus. This is the first successful report on artificial inoculation and plant development of Korean mistletoe.

Verification of aecial host ranges of four Gymnosporangium species based on artificial inoculation.

  • Yun, Hye-Young;Lee, Seung-Kyu;Lee, Kyung-Joon
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.134.1-134
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    • 2003
  • Aecial host ranges of four Gymnosporangium species causing cedar-apple rust diseases, G. asiaticum, G. cornutum, 5. japonicum and G. yamadae, were investigated through artificial inoculation. Thirteen species of nine genera among Rosaceous plants, which have been reported as social hosts in Korea, were inoculated with fresh teliospores spores in early days of May of 2000 and of 2001, respectively. In the results, we re-confirmed that there was highly specific relationship between the rust species and aecial hosts and report new aecial hosts of four Gymnosporangium species. Teliospores of G. cornutum collected from Juniperus rigida successively produced spermogonia and aecia only on Sorbus alntifolia, the first report on host alteration of G. cornutum in Korea. Positive responses by teliospores of G. japonicum from J. chinenis of Suwon and from J. chinenis var. horizontalis of Jeju island were obtained only on P. villosa. Crataegus pinnatifida was confirmed as a new aecial host of G. viatium. Until this time, G. ymadae was believed to have Malus as the aecial host. However, teliospores of G. yamadae collected from J. chinensis var. kaizuka successively formed spermogonia and aecia on the leaves of Chaenomeles lagenaria, C. sinensis, Pyrus pyrtifolia var, culta, P. ussuriensis, Malus pumila and M. sileboldii. The date for maturation of spermogonia and aecia, and symptom development varied according to the rust fungi and aecial host plants, respectively.

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The Impact of Environmental and Host Specificity in Seed Germination and Survival of Korean Mistletoe [Viscum album var. coloratum (Kom.) Ohwi]

  • Lee, Bo Duck;Lee, Young Woo;Kim, Seong Min;Cheng, Hyo Cheng;Shim, Ie Sung
    • 한국자원식물학회지
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    • 제28권6호
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    • pp.710-717
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    • 2015
  • Humankind has been searching for medicinal materials from various plant sources in an attempt to treat disease. Mistletoe is one indubitable plant source for these materials due to its effectiveness in treating various diseases, but it has almost disappeared from the mountainous areas of Korea due to excessive harvesting. In this study, in order to select host tree species for Korean mistletoe [Viscum album var. coloratum (Kom.) Ohwi] by seed inoculation and to clarify the effect of host specificity among various tree species were conducted for the purpose of gaining basic information for the artificial cultivation of Korean mistletoe. Almost all the seeds of Korean mistletoe germinated in vitro at the temperature of 15℃. Among host trees used in this study, Prunus mume showed the highest parasitic affinity with inoculated Korean mistletoe, compared with any other host plants. However, treatment of hormones could not increase the low survival rate of Korean mistletoe on the host trees.

Identification of Aecial Host Ranges of Four Korean Gymnosporangium Species Based on the Artificial Inoculation with Teliospores Obtained from Various Forms of Telia

  • Yun, Hye-Young;Lee, Seung-Kyu;Lee, Kyung-Joon
    • The Plant Pathology Journal
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    • 제21권4호
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    • pp.310-316
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    • 2005
  • The objectives of this study were to identify the aecial host ranges of four Korean Gymnosporangium species, G. asiaticum, G. cornutum, Gjaponicum and Gyamadae, and to verify the morphological characteristics of telia as diagnostic keys to the species. Thirteen Korean Rosaceous woody species were artificially inoculated with teliospores obtained from Juniperus species. There was high specificity between telial and aecial hosts and the fungal species, providing the first experimental proof on host alternation of these rust fungi in Korea. Telia on the witches' broom and on the small galls were identified as new telial characteristics in G asiaticum and in G. yamadae, respectively. Aecial hosts of G. asiaticum and G. yamadae showed varying responses in their susceptibility and in the days required for formation and duration of spermogonia and aecia after inoculation. Four telial host species in Juniperus were confirmed for the first time in Korea, which include J. chinensis var. kaizuka, J. chinensis var. horizontalis and J. chinensis var. globosa for G. asiaticum; and J. chinensis var. kaizuka for G. yamadae.

지적보전시스템의 실시간 다중고장진단 기법 개발 (Development of Multiple Fault Diagnosis Methods for Intelligence Maintenance System)

  • 배용환
    • 한국안전학회지
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    • 제19권1호
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    • pp.23-30
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    • 2004
  • Modern production systems are very complex by request of automation, and failure modes that occur in thisautomatic system are very various and complex. The efficient fault diagnosis for these complex systems is essential for productivity loss prevention and cost saving. Traditional fault diagnostic system which perforns sequential fault diagnosis can cause catastrophic failure during diagnosis when fault propagation is very fast. This paper describes the Real-time Intelligent Multiple Fault Diagnosis System (RIMFDS). RIMFDS assesses current machine condition by using sensor signals. This system deals with multiple fault diagnosis, comprising of two main parts. One is a personal computer for remote signal generation and transmission and the other is a host system for multiple fault diagnosis. The signal generator generates various faulty signals and image information and sends them to the host. The host has various modules and agents for efficient multiple fault diagnosis. A SUN workstation is used as a host for multiple fault modules and agents for efficient multiple fault diagnosis. A SUN workstation is used as a host for multiple fault diagnosis and graphic representation of the results. RIMFDS diagnoses multiple faults with fast fault propagation and complex physical phenomenon. The new system based on multiprocessing diagnoses by using Hierarchical Artificial Neural Network (HANN).

인공사료로 키운 회양목명나방의 세대별 발육 특성 (Growth of the Box Tree Pyralid, Glyphodes perspectalis Reared on an Artificial Diet Through Four Generations)

  • 박일권
    • 한국산림과학회지
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    • 제101권2호
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    • pp.305-308
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    • 2012
  • 회양목과 INSECTA F-II(Nihon Nosan Co., Ltd)를 3:7 비율로 섞은 인공사료를 이용하여 회양목명나방을 사육하면서 1~4세대까지의 발육특성을 조사하였다. 인공사료로 키운 회양목명나방의 유충기간은 기주식물인 회양목 잎을 이용하여 키웠을 때보다 더 길었다. 인공사료로 키운 세대 중 1세대와 나머지 세대 간에도 유충기간에 통계적으로 유의한 차이를 보였다. 용화율은 4세대에서 가장 높았으며 다음으로 3, 2, 1세대 순이었다. 번데기 기간은 1세대와 3세대에서만 통계적으로 유의한 차이를보였다. 우화율은 4세대에서 가장 높았으며 3, 1, 2세대 순이었다. 암수 성충의 크기는 1세대와 4세대 간에 유의한 차이가 보였다. 본 실험에서는 인공사료를 이용하여 회양목명나방 사육이 연중 가능하다는 것을 보여주었고, 4세대에 가면 인공사료에 대한 적응이 완전히 이루어진다는 것을 알 수 있었다.

내부기생충의 진화과정을 모방한 인공적응 모형 (An Artificial Adaptation Model by Means of the Endoparasitic Evolution Process)

  • 김여근;이효영;김재윤
    • 대한산업공학회지
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    • 제27권3호
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    • pp.239-249
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    • 2001
  • Competitive coevolution models, often called host-parasite models, are searching models that imitate the biological coevolution that is a series of reciprocal changes in two competing species. The models are known to be an effective method of solving complex and dynamic problems such as game problems, neural network design problems and constraint satisfaction problems. However, previous models consider only ectoparasites that live on the outside of the host when designing the models, not considering endoparasites that live on the inside of the host. This has a limitation to exploiting some information. In this paper, we develop an artificial adaptation model simulating the process in which hosts coevolve with both ectoparasites and endoparasites. In the model, the endoparasites play important roles as follows. By means of them, we can keep the history on results of previous competition between hosts and parasites, and use endogeneous fitness, not exogeneous. Extensive experiments are carried out to show the coevolution phenomenon and to verify the performance of the proposed model. Nim game problems and neural network problems are used as test-bed problems. The results are reported in this paper.

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간질피낭유충(肝蛭被囊幼蟲)의 생산(生産)에 관한 연구(硏究) (Study on Metacercarial Productivity of Fasciola sp. in its Intermediate Host, Austropeplea ollula (Lymnaea ollula))

  • 장두환;윤희정;전계식
    • 대한수의학회지
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    • 제27권2호
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    • pp.291-299
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    • 1987
  • The cattle liver flukes were collected at daughter house and classified with gross morphology. The hepatica type was only 14 flukes (4.7%) and the gigantica type was 284 flukes (95.3%) among total 298 flukes collected. In order to determinate the intermediate host of the flukes, artificial infection with the miracidia to snail hosts, Radix auricularia and Austropelea ollula, were successfully carried out, but the development in the later was terminated at the sporocyst stage. Intermediate host of the cattle flukes used to hibernate in deep part of the stubbles of rice pady. The number of A. ollula that has been collected from a rice stubble was ranged 0 to 19. Their mean number was 7 snails per the stubble. In artificial infection with miracidia, the reasonable number of them were 1 to 9 and the snail size was 2 to 5mm in length. The productivility of metacercaria in the intermediate host was observed. The metacercarial production per snail was ranged 5.5 to 48.0 per one miracidium.

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In Vitro Rearing of parasitoids of Insect Pests in China

  • Li, Li-Ying
    • 한국응용곤충학회지
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    • 제31권3호
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    • pp.241-246
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    • 1992
  • Since 1975, the studies on in vitro rearing Trichogramma spp., Anastatus japonicus Ashmead, Telenomus dendrolimusi Chu, Dibrachys cavus Walker, Habrobracon hebetor(Say), Bracon greeni Ashmead have been conducted successfully in China. The simulated “host-eggs" are made of polyethylene or polypropylene semispherical capsules, containing artificial diets, in which insectan pupal hemolymph is the main component. Manufacture of simulated “host larvae" are made of small rectangular parafilm of cotton-paper bags, containing artificial diets with insectan pupal hemolymph as the main component. Mass production of in vitro reared Trichogramma spp. and Anastatus japonicus and its utilization in the fields showed good effectiveness in controlling cotton bollworm, pine caterpillars, sugarcane borers and litchi stink bug.

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