• Microbiology and Biotechnology Letters
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• v.40 no.4
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• pp.396-402
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• 2012

The chemical composition, anti-porcine epidemic diarrhea virus (PEDV) activity and antimicrobial activity of Artemisia dubia essential oil were evaluated in this study. Fifty eight compounds from A. dubia essential oil were identified through analysis by gas chromatography-mass spectrometry (GC-MS). The major constituents of the oil were camphor (17.18 %), germacrene-D (15.70%), trans (${\beta}-$) racaryophyllene (6.79%), ene thujones (6.57%), 1, 8-cineole (5.94%) and camphene (5.08%). The essential oil was evaluated for antiviral activity against PEDV in Vero cells using a cytopathic effect (CPE) reduction method. The oils actively inhibited PEDV replication with a 50% inhibitory concentration ($IC_{50}$) of 43.7 ${\mu}^3/mL$. The 50% cytotoxicity concentration ($CC_{50}$) of the oils was over 100 ${\mu}/mL$ and the derived therapeutic index was >2.3. Similar analysis of the ribavirin revealed that they have a relatively weaker efficacy when compared to the oils. The antimicrobial activity of the essential oil against 5 microorganisms was evaluated by the disc diffusion method. The essential oil exhibited antimicrobial activity against 5 tested microorganisms with a clear zone of 8-22 mm. Among the tested microorganisms, Streptococcus pyogenes was the most sensitive and Candida albicans the least. Therefore, in can be concluded that essential oils of A. dubia may have interesting applications for microbial control or the control of PEDV-derived diseases.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.6-6
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• 2023

Nosemosis is one of the most common protozoan diseases of adult bees (Apis mellifera). Nosemosis is caused by two species of microsporidia; Nosema apis and Nosema ceranae. Nosema ceranae is potentially more dangerous because it has the ability to infect multiple cell types, and it is now the predominant microsporidian species in A. mellifera. In this study, we identified two anti-nosemosis plants, Aster scaber and Artemisia dubia, which reduced the spore development of N. ceranae in spore-infected cells. We intend to establish the anti-nosemosis activity of aqueous, ethyl acetate (EA), and butanol (BuOH) extracts of A. dubia and A. scaber. In order to determine the optimal dose, we did in vitro and in vivo toxicity for all the extracts and carried out anti-nosemosis experiments. Although all of the extracts (aqueous, EA, and BuOH) showed in vitro and in vivo anti-nosemosis activity in a dose-dependent manner, the aqueous extracts of A. dubia and A. scaber showed more potent anti-nosemosis activity than the EA and BuOH extracts. And then, we isolated five phenolic compounds [chlorogenic acid, 3,4-dicaffaeoylquinic acid (3,4-DCQA), 3,5-dicaffaeoylquinic acid (3,5-DCQA), 4,5-dicaffaeoylquinic acid (4,5-DCQA), and coumarin] from A. dubia, A. scaber, and A. dubia + A. scaber aqueous extracts and screened for their toxicities and anti-Nosema effects in both in vivo and in vitro conditions. Among these five compounds, coumarin, chlorogenic acid, and 4,5-DCQA exhibited less toxic but more potent anti-Nosema effects than the other two compounds. Especially, chlorogenic acid and coumarin showed prominent anti-Nosema activities even at the lowest concentration (10 ㎍/mL). They might have potential to be developed as alternative compounds for the control of Nosema disease.

• Journal of Integrative Natural Science
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• v.4 no.1
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• pp.15-22
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• 2011

In this study, liquid with carbonized artemisia was developed for the first time that can be used as food additives and outside medicinal agents by burning artemisia species such as Artemisia dubia wall, Artemisia capillaris Thunb, Artemisia montana (Nakai) Pamp), Artemisia keiskeana Miq, Leonurus Japonicus Houtt. etc. In addition, edibility of recovered liquid with carbonized artemisia was verified by comparing the contents according to each ingredient with common refined pyroligneous liquid through the analysis of 9 kinds of organic acid by the species of artemisia, methanol and ethanol. Besides, the result of antimicrobial activities on six kinds of food spoilage and food poisoning bacteria observed by paper disc method for liquid with carbonized artemisia indicated that those two liquids showed strong antimicrobial. At the concentration of 1.5 mg/disc, clear zone for S. sonnei and S. aureus, L. monocytogenes was 13~16 mm, and they showed stronger antimicrobial activities than other strain.

• Korean Journal of Plant Resources
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• v.23 no.4
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• pp.293-302
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• 2010

Taxa of Artemisia collected in Korea were constructed by molecular phylogenetic analysis based on the internal transcribed spacer(ITS) regions of nrDNA. The length of the ITS sequences aligned using the clustal X program was 636~643 bp, and the lengths of the ITS1 and ITS2 regions were 251~255 bp and 217~222 bp, respectively. The total number of variable sites was 95 for the entire sequence, and a parsimony- informative site represented an efficacious site in ITS1 rather than in ITS2. The maximum parsimony tree as calculated by the MEGA 4 program was clustered into five clades. The taxa(A. capillaris, A. japonica var. japonica, A. japonica var. hallaisanensis, A. japonica subsp. littoricora) degenerated ovary of clade 1 was supported as the subgenus Dracunculus by Ling's classification system. The results show that A. nakaii and A. fukudo were quite similar genetically(Boostrap 99%) and that the scientific name of Korean A. dubia should be reconsidered. A. sp. distributed in Ganghwa province was grouped with A. argyi(Boostrap 89%). These results suggest that the molecular techniques used in this study could be useful for the phylogenetic analysis of Korean Artemisia herbs having variations in their morphological characteristics.

• Journal of Food Hygiene and Safety
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• v.35 no.4
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• pp.341-353
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• 2020

In accordance with the implementation of the Positive List System (PLS), the proper usage of pesticides is now being enforced. It is assumed that unregistered pesticides are being used on agroforestry products due to the low number of registered pesticides in the agricultural industry. In this study, pesticide and heavy metal residues were investigated in 13 types of products to determine the status of usage. The levels (%) of pesticides detected in Pimpinella beachscape, Platycodon grandiflorum, Codonopsis lancekolate, Artemisia dubia, Angelica gigas, Pyrus pyrifolia, and Punica granatum were 40.0, 20.0, 26.7, 13.3, 56.3, 57.1, 33.3, 26.7, 66.7, and 46.7%, respectively, while, those in Petridium aquilinum, Disoscorea batata and Senna tora were zero. Heavy metals (Pb, Cd) were detected only from P. grandisflorum and A. dubia. The pesticide usage and registration data by agroforestry product obtained in the study will be useful in the future for ensuring the safety of domestic agroforestry products.

• Journal of Environmental Science International
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• v.29 no.6
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• pp.579-603
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• 2020

In order to provide fundamental information about the floristic composition of the area along with an assessment of the environmental impact, a floristic study of the vascular plants in the Tamjin River estuary in Gangjin-gun was conducted for a total of nine days, in the period from June 2014 to September 2014. We found that the vascular plants in this region comprised 424 taxa belong to 102 families, 281 genera, 390 species, 5 subspecies, 26 varieties, and 3 forma. Five taxa of Korean endemic plants including Weigela subsessilis (Nakai) L.H.Bailey, Lespedeza maximowiczii var. tricolor (Nakai) Nakai, and Clematis trichotoma Nakai were collected. Two least concern (LC) taxa of rare plants (as designated by the Korea Forest Service) were collected: Hydrocharis dubia (Blume) Backer and Platycladus orientalis (L.) Franco. Ten the floristic regional indicator taxa from the third to the fifth grade were identified: two taxa belonged to grade IV, and eight taxa belonged to grade III. Twenty-four taxa of salt-tolerant plants, including Artemisia fukudo Makino, Carex rugulosa Kuk., and Suaeda glauca (Bunge) Bunge, as well as 44 taxa of aquatic plants, including Najas marina L., Nuphar oguraensis Miki, and Nymphoides indica (L.) Kuntze, were investigated in this region. Fifty-nine taxa of naturalized plants were recorded, among which the following six taxa were plants that caused ecosystem disturbance: Ambrosia artemisiifolia L., Humulus scandens (Lour.) Merr., Lactuca scariola L., Rumex acetosella L., Solidago altissima L., and Symphyotrichum pilosum (Willd.) G.L.Nesom.