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A phylogenetic analysis of Korean Artemisia L. based on ITS sequences  

Lee, Jeong-Hoon (Department of Herbal Crop Research NIHHS, RDA)
Park, Chung-Berm (Department of Herbal Crop Research NIHHS, RDA)
Park, Chun-Geon (Department of Herbal Crop Research NIHHS, RDA)
Moon, Sung-Gi (Department of Biology, Kyungsung University)
Publication Information
Korean Journal of Plant Resources / v.23, no.4, 2010 , pp. 293-302 More about this Journal
Abstract
Taxa of Artemisia collected in Korea were constructed by molecular phylogenetic analysis based on the internal transcribed spacer(ITS) regions of nrDNA. The length of the ITS sequences aligned using the clustal X program was 636~643 bp, and the lengths of the ITS1 and ITS2 regions were 251~255 bp and 217~222 bp, respectively. The total number of variable sites was 95 for the entire sequence, and a parsimony- informative site represented an efficacious site in ITS1 rather than in ITS2. The maximum parsimony tree as calculated by the MEGA 4 program was clustered into five clades. The taxa(A. capillaris, A. japonica var. japonica, A. japonica var. hallaisanensis, A. japonica subsp. littoricora) degenerated ovary of clade 1 was supported as the subgenus Dracunculus by Ling's classification system. The results show that A. nakaii and A. fukudo were quite similar genetically(Boostrap 99%) and that the scientific name of Korean A. dubia should be reconsidered. A. sp. distributed in Ganghwa province was grouped with A. argyi(Boostrap 89%). These results suggest that the molecular techniques used in this study could be useful for the phylogenetic analysis of Korean Artemisia herbs having variations in their morphological characteristics.
Keywords
Artemisia L.; Phylogenetic analysis; ITS; Dracunculus;
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