Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Anti-Porcine Epidemic Diarrhea Virus (PEDV) Activity and Antimicrobial Activities of Artemisia dubia Essential Oil

참쑥(Artemisia dubia) 오일의 돼지 유행성 설사 바이러스(Porcine Epidemic Diarrhea Virus)에 대한 항바이러스 항균활성

  • Received : 2012.08.29
  • Accepted : 2012.10.30
  • Published : 2012.12.28
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Abstract

The chemical composition, anti-porcine epidemic diarrhea virus (PEDV) activity and antimicrobial activity of Artemisia dubia essential oil were evaluated in this study. Fifty eight compounds from A. dubia essential oil were identified through analysis by gas chromatography-mass spectrometry (GC-MS). The major constituents of the oil were camphor (17.18 %), germacrene-D (15.70%), trans (${\beta}-$) racaryophyllene (6.79%), ene thujones (6.57%), 1, 8-cineole (5.94%) and camphene (5.08%). The essential oil was evaluated for antiviral activity against PEDV in Vero cells using a cytopathic effect (CPE) reduction method. The oils actively inhibited PEDV replication with a 50% inhibitory concentration ($IC_{50}$) of 43.7 ${\mu}^3/mL$. The 50% cytotoxicity concentration ($CC_{50}$) of the oils was over 100 ${\mu}/mL$ and the derived therapeutic index was >2.3. Similar analysis of the ribavirin revealed that they have a relatively weaker efficacy when compared to the oils. The antimicrobial activity of the essential oil against 5 microorganisms was evaluated by the disc diffusion method. The essential oil exhibited antimicrobial activity against 5 tested microorganisms with a clear zone of 8-22 mm. Among the tested microorganisms, Streptococcus pyogenes was the most sensitive and Candida albicans the least. Therefore, in can be concluded that essential oils of A. dubia may have interesting applications for microbial control or the control of PEDV-derived diseases.

이 연구에서 참쑥(Artemisia dubia) 오일의 화학적 조성, 돼지 유행성 설사 바이러스(porcine epidemic diarrhea virus, PEDV)에 대한 항바이러스 활성 및 항균 활성을 평가하였다. 참쑥 오일로부터 질량 분석기가 장착된 가스 크로마토 그래피(gas chromatography-mass spectrometry, GC-MS)에 의해 58개의 화합물이 분석되어졌다. 그 오일의 주요한 성분은 각각 camphor (17.18%), germacrene-D (15.70%), trans(${\beta}-$)caryophyllene (6.79%), ${\beta}$-thujones (6.57%), 1, 8-cineole (5.94%) and camphene (5.08%) 순으로 나타났다. 참쑥 오일의 돼지 유행성 설사 바이러스에 대한 항바이러스능을 세포독성 감소 방법 [cytopathic effect (CPE) reduction method]에 의해 베로 세포(원숭이 신장세포, Vero cells)에서 평가하였다. 그 오일은 43.7 ${\mu}g/ml$의 50% 저해농도 ($IC_{50}$)와 함께 돼지 유행성 설사 바이러스 복제를 억제하였다. 또한 그 오일은 100 ${\mu}g/ml$ 이상에서 세포독성을 나타내지 않았으며, 그 치료지수(therapeutic index, TI value)는 2.3 이상이였다. 리바비린(rivabirin)은 그 오일에 비해 상대적으로 낮은 항바이러스능을 나타내었다. 다섯개 미생물(Staphyloscoccus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Propionibacterium acnes, Candida albicans)에 대한 참쑥 오일의 항균 활성은 페이퍼 디스크 방법(paer disck diffusion method)에 의해 평가하였다. 그 결과 그 오일은 5가지 미생물에 대해 8-22 mm의 투명환(clear zone)을 나타내며 항균활성을 보였다. 테스트된 미생물들 가운데 S. pyogenes가 가장 높은 활성을 나타냈으며, C. albicans가 가장 낮은 활성을 나타냈다. 이상의 결과로부터 참쑥 오일은 항균 및 돼지 유행성 설사 바이러스 유래 질병을 조절하는데 응용 가능할 것이라 사료된다.

Keywords

References

  1. Adams, R. P. 2001. Identification of essential oils components by gas chromatography/quadrupole mass spectroscopy, Allured Publishing Corporation, Illinois, USA.
  2. Blagojevic, P., N. Radulovic, R. Palic, and G. Stojanovic. 2006. Chemical composition of the essential oils of serbian wild-growing Artemisia absinthium and Artemisia vulgaris. J. Agric. Food Chem. 54: 4780-4789. https://doi.org/10.1021/jf060123o
  3. Burt, S. A. 2004. Essential oils: their antibacterial properties and potential applications in foods-a review. Int. J. Food Microbiol. 94: 223-253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
  4. Chagonda, L. S., C. Makanda, and J. C. Chalchat. 1999. The essential oil of cultivated Artemisia afra (Jacq.) from Zimbabwe. Flav. Fragr. J. 14: 140-142. https://doi.org/10.1002/(SICI)1099-1026(199903/04)14:2<140::AID-FFJ800>3.0.CO;2-R
  5. Chericoni, S., G. Flamini, E. Campeol, P. L. Cioni, and I. Morelli. 2004. GC-MS analyses of the essential oil from the aerial parts of Artemisia verlotiorum: variability during the year. Biochem. Syst. Ecol. 32: 423-429. https://doi.org/10.1016/j.bse.2003.10.002
  6. Choi, H. J., J. H. Kim, C. H. Lee, Y. J. Ahn, J. H. Song, and S. H. Baek. 2008. Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus. Antiviral Res. 81: 77-81.
  7. Conner, D. E. 1993. Naturally occurring compounds, pp. 441-468. In P. M. Davidson, and A. L. Branen (eds.), Antimicrobials in foods, NewYork, Marcel Dekker.
  8. Deans, S. G. and P. G. Waterman. 1993. pp. 113. In R. K. M. Hay, and P. G. Waterman (eds.), Volatile Oil Crops: TheirBiology, Biochemistry and Production, London, Longman.
  9. Denyer, S. P. and W. B. Hugo. 1991. Biocide-induced damage to the bacterial cytoplasmic membrane, pp. 171-188. In Denyer, S. P. and W. B. Hugo (eds.), Mechanisms of Action of Chemical Biocides, The Society for Applied Bacteriology, Technical Series. Oxford Blackwell Scientific Publication, Oxford.
  10. Dorman, H. J. D. and S. G. Deans. 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J. Appl. Microbiol. 88: 308-316. https://doi.org/10.1046/j.1365-2672.2000.00969.x
  11. Juteau, F., V. Masotti, J. M. Bessiere, M. Dherbomez, and J. Viano. 2002. Antibacterial and antioxidant activities of Artemisia annua essential oil. Fitoterapia 73: 532-535. https://doi.org/10.1016/S0367-326X(02)00175-2
  12. Kalemba, D., D. Kusewicz, and K. Swiader. 2002. Antimicrobial properties of the essential oil of Artemisia asiatica Nakai. Phytother. Res. 16: 288-291. https://doi.org/10.1002/ptr.856
  13. Khaled, F., A. El-Massry, H. El-Ghorab, and A. Farouk. 2002. Antioxidant activity and volatile components of Egyptian Artemisia judaica L. Food Chem. 79: 331-336. https://doi.org/10.1016/S0308-8146(02)00164-4
  14. Khan, M. T. H., A. Ather, K. D. Thompson, and A. Gambari. 2005. Extracts and molecules from medicinal plants against herpes simplex viruses. Antiviral Res. 67: 107-119. https://doi.org/10.1016/j.antiviral.2005.05.002
  15. Khazraei-Alizadeh, K. and A. Rustaiyan. 2001. Composition of the volatile oil of Artemisia diffusa Krasch ex Poljak growing wild in Iran. J. Essent. Oil Res. 13: 185-186. https://doi.org/10.1080/10412905.2001.9699656
  16. Kordali, S., A. Cakir, A. Mavi, H. Kilic, and A. Yildirim. 2005. Screening of chemical composition and antifungal and antioxidant activities of the essential oils from three Turkish Artemisia species. J. Agric. Food Chem. 53: 1408-1416. https://doi.org/10.1021/jf048429n
  17. Kordali, S., R. Kotan, A. Mavi, A. Cakir, A. Ala, and A. Yildirim. 2005. Determination of the Chemical Composition and Antioxidant Activity of the Essential Oil of Artemisia dracunculus and of the Antifungal and Antibacterial Activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum, and Artemisia spicigera Essential Oils. J. Agric. Food Chem. 53: 9452-9458. https://doi.org/10.1021/jf0516538
  18. Loizzo, M. R., A. Saab, R. Tundis, G. A. Statti, I. Lampronti, F. Menichini, R. Gambari, J. Cinatl, and H.W. Doerr. 2008. Phytochemical analysis and in vitro evaluation of the biological activity against herpes simplex virus type 1 (HSV- 1) of Cedrus libani A. Rich. Phytomed. 15: 79-83. https://doi.org/10.1016/j.phymed.2007.03.013
  19. Murray, P. R., E. J. Baron, M A. Pfalletr, F. C. Tenover, and R. H. Yolke. 1995. Manual of clinical microbiology, 6th Ed. ASM, Washington, DC.
  20. Pensaert, M. B. 1999. Porcine epidemic diarrhea, (pp. 179- 185). In B. E. Straw (eds.). Diseases of Swine. Iowa State University Press, Iowa, EEUU.
  21. Pino, J. A., A. Rosado, M. T. Correa, and V. Fuentes. 1996. Chemical composition of the essential oil of Artemisia dracunculus L. from Cuba. J. Essent. Oil res. 8: 563-564. https://doi.org/10.1080/10412905.1996.9700690
  22. Schnitzler, P., K. Schon, and J. Reichling. 2001. Antiviral activity of Australian tea tree oil and eucalyptus oil against herpes simplex virus in cell culture. Pharmazie 56: 343-347.
  23. Schuhmacher, A., J. Reichling, and P. Schnitzler. 2003. Virucidal effect of peppermint oil on the enveloped viruses herpes simplex virus type 1 and type 2 in vitro. Phytomed. 10: 504-510. https://doi.org/10.1078/094471103322331467
  24. Sikkema, J., J. A. M. De Bont, and B. Poolman. 1994. Interactions of cyclic hydrocarbons with biological membranes. J. Biol. Chem. 269: 8022-8028.
  25. Singh, A. P., M. S. Bista, M. K. Adhikari, and K. K. Rajbhandary. 2001. Flowering plants of Nepal (Phanerogams), Bull. Dept. of Plant resources, pp. 3999. Ministry of Forests and Soil conservation, Department of Plant resources, National Herbarium and Plant Laboratories, Godavary, Lalitpur, Nepal.
  26. Song, D. S., J. S. Oh, B. K. Kang, J. S. Yang, H. J. Moon, H. S. Yoo, Y. S. Jang, and B.K. Park. 2007. Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain. Res. Vet. Sci. 82: 134-140. https://doi.org/10.1016/j.rvsc.2006.03.007

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