• Proceedings of the Korean Nuclear Society Conference
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• 1998.05b
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• pp.720-724
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• 1998

감마선이 Artemia 알의 부화에 미치는 영향을 알아보고자, 10Gy, 100Gy, 3kGy, 6kGy과 9kGy의 선량으로 건조 상태의 알을 조사한 후 부화율과 nauplius기의 출현률을 조사하였다. 1kGy 이하의 방사선 조사군에서는 부화율과 nauplius기의 출현률이 대조군에 비하여 높게 나타났다. 3kGy 방사선조사군의 경우 대조군과 유사한 부화율을 보였으나 nauplius기의 출현률은 감소하였다. 6kGy의 방사선조사군에서는 알들의 부화 지연과 부화율의 감소가 뚜렷하였다. 50％의 부화율을 나타내는 선량은 약 5.5kGy였으며, 9kGy에서는 100％ 치사선량을 나타내었다. 본 실험에서 조사한 선량증에서 비교적 낮은 선량의 범위에서는 이온화방사선에 의하여 Artemia 알의 부화시간 단축 및 부화율이 다소 증가하는 결과를 보였다. 따라서 이온화방서선을 이용한 Artemia 알의 부화률을 촉진지킬 수 있는 적정 선량에 대한 연구는 상업적으로도 가치가 있다고 생각되며, 향후 이에 대한 추가적인 연구를 통한 상업적인 활용이 기대된다.

• Journal of Aquaculture
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• v.22 no.1
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• pp.100-104
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• 2009

This study was conducted to evaluate the effect of dietary supplementation of paprika powder as carotenoid source with three different zooplankton on carotenoid deposition and skin pigmentation of rose bitterling Rhodeus ocellatus. Two replicate groups of fish (initial average weight 0.8 g) were fed the five experimental diets containing 5% paprika (P5) and mixture of 5% paprika with 3% three zooplankton of water flea Moina macrocopa (P5M3), Artemia nauplius Artemia sp. (P5A3) and rotifer Brachionus plicatilis (P5R3) and without supplementation of carotenoid source (Con) for 10 weeks. Survival of fish among the treatments was not affected by dietary supplementation. Total carotenoid contents of whole body in fish fed the diets containing 3% zooplankton was higher than that of fish fed other diets. Especially, the highest total carotenoid contents of whole body was observed in fish fed the P5A3 diet. Similarity, Lightness, $a^*$ and $b^*$ values of the fish fed the diets containing 3% zooplankton tended to be higher than that in fish fed control and P5 diets. The apparent color intensity of skin and fin in fish fed the diets containing paprika with Artemia nauplius and rotifer was better than those of fish fed other diets. The results of this study suggest that dietary supplementation of paprika with Artemia nauplius and rotifer can improve skin pigmentation of rose bitterling.

• Journal of Aquaculture
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• v.7 no.3
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• pp.135-150
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• 1994

For the effective seedling production of the sweetfish, Plecoglossus altivelis, dietary value of live food in the growth and survival rate of the larvae was examined. To investigate dietary value of three types of rotifers (L-type, S-type, Us-type rotifer) for the sweetfish larvae, the larvae were fed with alone or mixed with each type and with mixtures of two or three types. Although the survival rates of the larvae fed different types of rotifers were not significantly different among them, the larvae fed a mixture of three types of rotifers showed the highest growth significantly. A feeding trial was conducted to determine the effect of the dietary values of rotifer and Artemia nauplius enriched with Chlorella ellipsoidea, $\omega-yeast$, spray-dried Spirulina platensis, and Super Selco for the sweetfish larvae. The dietary values of rotifer and Artemia nauplius enriched with Super Selco mixed with spray-dried S. platens is were distinctly improved for the survival rate and growth of the larvae. With regard to effect of the green water with Chlorella ellipsoidea, spray-dried Spirulina platensis, and the addition of photosynthetic bacteria (Rhodopseudomonas capsulatus), the green water with Spirulina platens is mixed with photosynthetic bacteria also enhanced the growth and survival rate of the larvae.

• Fisheries and Aquatic Sciences
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• v.8 no.4
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• pp.201-206
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• 2005

Microalgae are widely used for mass culture of the rotifer Brachionus plicatilis in aquaculture. Since the nutritional value of the rotifer is closely related to its food, the nutritional value of its food should be known in detail. Chlorella ellipsoidea and Nannochloris oculata are re­presentative food organisms for rotifers that are easily cultured. Therefore, the nutritional values of these micro algae were examined for ultrasmall, small, and large rotifers and Artemia nauplii. Chlorella ellipsoidea contained seven times more total fatty acids than N. oculata. The three types of rotifer fed N. oculata contained more amino acids than those fed C. ellipsoidea. However, the total fatty acids of the rotifers fed each microalga species differed according to the type of rotifer. Newly hatched Artemia nauplii contained more protein and had a higher dry weight than those fed microalgae for 6 h. As with the rotifers, the Artemia nauplii fed N. oculata contained more protein and amino acids than those fed C. ellipsoidea, while the reverse was true for the total fatty acid content. Our results suggest that N. oculata is a good supply of protein, while C. ellipsoidea is a good source of lipids as food organisms for rotifers and Artemia nauplii in aquaculture.

• Journal of the Korean Society for Marine Environment & Energy
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• v.12 no.4
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• pp.302-306
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• 2009

9 kinds of nanoparticle used for this study was a particle with the size of less than 100 nm of diameter, and Artemia sp. cyst examined what kind of influence to have upon the process hatched out in nauplius. 82% hatched in nauplius at the opposition ward where a nanoparticle wasn't added after 24 time course. AGZ020, Nano silver, P-25, Sb and SnO nanoparticle showed hatching rate of 18%, 20%, 13%, 50% and 0% respectively by the 20mg/L density, and it became clear that a harmful effect is big, but I had a harmful effect compared with the opposition ward by 75%, 60%, 73% and 73% respectively by Ag-$TiO_2$, In, Sn and Zn nanoparticle, but a feeble thing was known relatively compared with AGZ020, Nano silver, P-25, Sb and SnO nanoparticle. The difference has caused this with the ingredient a nanoparticle has. Ag is included 2 % and AGZ020, Nano silver and P-25 nanoparticle are used widely as anti-fungus agent, and the SnO nanoparticle which became combination is a light catalyst pill, and oxygen is used for a Sn particle. This and others, a possibility that use is generalized and flows into aquatic environment in sequence the home electronics, functionality cosmetics, anti-fungus agent and a light catalyst pill at present becomes high for nanoparticles and others. The anxiety which has an influence on the ecology world in the water with this can be generated, so I'd have to study the potential danger a nanoparticle has continuously.

• Proceedings of KOSOMES biannual meeting
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• 2008.05a
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• pp.137-141
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• 2008

9 kinds of nanoparticle used for this study was a particle with the size of less than 100 nm of diameter, and Artemia sp. cyst examined what kind a influence to have upon the process hatched out in nauplius. 82% hatched in nauplius at the opposition ward where a nanoparticle wasn't added after 24 time course. AGZ020, Nano silver, P-25, Sb and SnO nanoparticle showed hatching rate of 18%, 20%, 13%, 50% and 0% respectively by the 20mg/L density, and it became clear that a harmful effect is big, but I had a harmful effect compared with the opposition ward by 75%, 60%, 73% and 73% respectively by Ag-$TiO_2$, In, Sn and Zn nanoparticle, but a feeble thing was known relatively compared with AGZ020, Nano silver, P-25, Sb and SnO nanoparticle. The difference was mused this with the ingredient a nanoparticle has. Ag is included 2% and AGZ020, Nano silver and P-25 nanoparticle are used widely as anti-fungus agent, and the SnO nanoparticle which became combination is a light catalyst pill, and oxygen is used for a Sn particle. This and others, a possibility that use is generalized and flows into aquatic environment in sequence the home electronics, functionality cosmetics, anti-fungus agent and a light catalyst pill at present becomes high for nanoparticles and others. The anxiety which has an influence on the ecology world in the water with this can be generated, so I'd have to study the potential danger a nanoparticle has continuously.

• Journal of Aquaculture
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• v.13 no.3
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• pp.197-206
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• 2000

At the Poryoung coast, the spawning season of the mullet, Chelon lauvergnii lasts from May to June. A combination of rotifer, Rotifer nauplii and synthetic feed supported significantly (P < 0.05) better survival and growth of 1-day old mullet larvae during 70-day rearing experiment than the other feeds consisting of rotifer or rotifer + Artemia nauplii. Significantly higher growth and survival were also observed, when the larvae were fed on rotifer, that were harvested from culture enriched with masimaTR, or Artemia nauplii from culture enriched with cuttlefish liver oil, At the feeding density of 97-98 individuals /200 l, the mullet larvae displayed maximum growth and survival in aquarium each with 100 larvae /200 l.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.84-85
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• 2003

조개류의 먹이생물인 미세조류를 대체할 수 있는 효모를 개발하기 위하여 빵효모 (Saccharomyces cerevisiae), Candida utilis, Klyveromyces fragilis 3종류의 효모를 대상으로 그 먹이효율을 실험하였다. 이들 3종류의 효모를 세포벽을 75, 50, 25% 제거한 것과 세포벽을 제거하지 않은 효모로 구분하여 Artemia를 대상으로 먹이효율을 조사한 후 가장 좋은 먹이효율을 보인 C. utilis를 조개류의 대표적 먹이생물인 Isoch교sis galbana 와 함께 진주담치 유생을 대상으로 먹이효율을 조사하였다. Artemia 실험결과 빵효모보다 C. utilis, K. fragilis가 높은 생존율과 성장을 보였으며 세포벽을 제거한 것이 제거하지 않은 것보다 유의적으로 높은 생존율을 보였다. Artemia의 생존율은 75%와 50% 세포벽을 제거한 것이 25%에 비해 유의적으로 높은 생존율을 보였다. 성장은 유의적이지는 않지만 전체적으로 세포벽을 많이 제거할수록 성장과 생존율은 높은 경향이었다. 이와같이 Artemia의 경우는 세포벽을 제거한 C. utilis가 더 좋은 먹이효율을 보인 것은 Artemia는 효모의 세포벽을 소화시키지 못하기 때문으로 판단 할 수 있다. 진주담치 유생의 먹이로 C. utilis를 공급한 경우 성장에 있어서 세포벽을 제거한 효모를 50% 대체한 실험구의 먹이효율이 가장 좋았으며 세포벽을 벗기지 않은 효모의 경우 I. galbana에 비해 낮은 결과를 보였다. 진주담치 유생에 있어서도 세포벽을 제거한 C. utilis를 공급한 것은 대조구인 I. galbana와 생존율에 있어 유의성이 없었다. 그리고 25% 세포벽을 제거한 것을 공급한 실험구는 유의적으로 낮은 생존율을 보였다. 각장 성장의 경우 75% 세포벽을 제거한 것은 대조구인 I. galbana와 유의성이 없었다. 세포벽을 제거한 C. utilis와 I. galbana를 1:1로 혼합하여 공급한 실험의 경우 생존율에 있어서는 세포벽을 50%, 75% 제거한 C. utilis를 50% 첨가한 실험구는 대조구인 I. galbana 100%를 공급한 것과 유의적인 차이가 없었다. 그러나 성장의 경우는 I. galbana 에 세모벽을 75% 제거한 C. utilis를 1:1로 혼합한 실험구에서 각장 179.3 $\mu\textrm{m}$, 각고 150.3 $\mu\textrm{m}$로 가장 높았고 대조구인 I. galbana 100%와는 유의적인 차이를 보였다.

• Journal of Aquaculture
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• v.11 no.1
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• pp.99-103
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• 1998

Artemia often observed as a co-existing organism in the mass culture tank of marine rotifer. The rotifer and Atremia are commonly used as food organisms in the marine fish larvae rearing. In this study, interspecific relation between the rotifer Brachionus rotundiformis (formely called S-type) and anostracan Artemia of the two developmental stages (0 and 19 day old after hatching) were investigated in the larboratory. The population growth of B. rotundiformis and one of the stage (nauplius or adult) of Artemia in mixed culture was compared with that of each single species culture. Culture period was 16 days. Every two days, the number of organisms in each species was counted and transferred to a fresh medium containing $7{\times}10^5$cells/ml of food Nannochloropsis oculata. Culture volume, temperature, salinity and photoperiod were set at 40ml, $25^{\circ}C.$, 22ppt and 24h all dark except to observation time, respectively. The rotifer population growth was greatly decreased by co-existence with Artemia. The coexisting Artemia suppressed the rotifer population growth due to it's high filtering speed for food (N. oculata). This study suggested that contamination by Artemia must be prevented for the stable rotifer production in the rotifer mass culture tank.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.11
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• pp.682-687
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• 2018

This study investigated the food value of Paracyclopina nana for flounder Paralichthys olivaceus larvae in two feeding stages, rotifer (for 12 days) and Artemia (for 16 days). In the rotifer feeding stage, survival and growth of flounder larvae in the only P. nana (nauplii) feeding experiment were higher than in the only rotifer feeding experiment on 12 DAH (days after hatching). In the Artemia feeding stage, the growth of flounder larvae in the only P. nana (C4-adult) feeding experiment and mixture feeding experiment (P. nana+Artemia) were higher than in other experiments on 30 DAH, but the survival of flounder larvae did not differ significantly among experiments. The n-3 HUFA contents of nauplius and C4-adult were 4.0% and 5.4%, respectively. Overall the results of this study indicate that the brackish water cyclopoid copepod, P. nana, is an effective live food organism for larval seedling production of marine fish.