• BMB Reports
• /
• 제33권2호
• /
• pp.184-187
• /
• 2000

Prostaglandin $E_2$ ($PGE_2$) plays an important role in the regulation of various gastric functions, and the growth-inhibitory activities on tumor cells are studied in vitro and in vivo. Although the mechanisms have attracted many researchers in the past decade, the molecular mechanisms of cell cycle arrest, or induction of apoptosis by $PGE_2$, is unclear. We investigated the effects of $PGE_2$ on the growth of the human gastric carcinoma cell line SNU1 and genes that are regulated by $PGE_2$ and isolated them using differential display RT-PCR (DD RT-PCR). FACS analysis suggested that SNU1 cells were arrested at the G1 phase by $PGE_2$ treatment. This growth inhibitory effect was in a time- and dose-dependent manner. Treatment of SNU1 cells with $10\;{\mu}g/ml$ $PGE_2$, followed by DD RT-PCR analysis, revealed differently expressed bands patterns from the control. Among the differently expressed clones, we found an unidentified cDNA clone (HGP-27) overexpressed in $PGE_2$-treated cells. The full-length cDNA of HGP-27 was isolated using RACE, which consisted of a 30-nt 5'-noncoding region, a 891-nt ORF encoding the 296 amino acid protein, and a 738-nt 3'-noncoding region including a poly(a) signal. This gene was localized on the short arm of chromosome number 11. Using the Motif Finder program, a myb-DNA binding repeat signature was detected on the ORF region. The COOH-terminal half was shown to have similarity with the $NH_3$-terminal domain of thioredoxin (Trx). This relation between HGP-27 and Trx implied a potential role for HGP-27 in modulating the DNA binding function of a transcription factor, myb.

• 한국수산과학회지
• /
• 제47권5호
• /
• pp.459-473
• /
• 2014

The recent mass appearances of jellyfish in Korea have caused economic and social damage, as they plague swimmers and fishermen. However, jellyfish have high economic and nutritional value, and contain low levels of calories and hydrolysates. Thus, jellyfish are a natural, healthy food that can improve high blood pressure, bronchitis, and a multitude of other diseases. Here, we present research on the ecology, classification, bloom, damage caused, food component characterization, and tissues of jellyfish, with the aim of facilitating further study. Research on use of jellyfish as salted products, and for collagen and qniumucin would also be valuable. A jellyfish body is classified into three parts: the body, termed the umbrella; the oral arm; and the tentacle. Jellyfish are planktonic marine members of a group of invertebrate animals comprising the classes Schypozoa (phylum Cnidaria) and Cuboza. In Korea in 2012, jellyfish damage resulted in decreases of annual catch and commercial value estimated at 177 and 141 billion won, respectively. Because concentrations of heavy metals are below the safety limits for seafood, dried jellyfish appear to be safe raw materials for food. The proximate compositions of Nemopilema nomurai and Aurelia aurita were 97.1% and 96.5% moisture, 0.9% and 1.2% crude proteins, undetected and 0.1% crude lipids, and 1.7% and 1.8% ash, respectively. According to their total contents of essential, total, and non-essential amino acids, jellyfish gonads were deemed good-quality protein. Because the major functional components of jellyfish are collagen and qniumucin, jellyfish can be used salted, or these components of healthy diets can be extracted from them. For more effective use of jellyfish, unit costs should be decreased and safety guaranteed. Additionally, dehydrators attached to conveyor belts should be developed. Since jellyfish can be used throughout the year, they should be listed in the Korean Food Standards Codex as a food source.

• 기본간호학회지
• /
• 제5권2호
• /
• pp.280-292
• /
• 1998

Pressure sores are a serious concerns in that respect to increasing risk of medical complications and medical costs. Prevention and care of pressure sores is an essential area of nursing practice. The nurse at ICU should be more careful of maintaining the skin integrity of patients especially than at any other place. This study was conducted to determine if the risk facotrs of pressure sores and nutritional status of the patients at risk for pressure sores is related the occurance of pressure sore. The risk group refers the patients having the below 14 scores of the braden scale. The 100 subjects were recruited from the ICU ward at an university hospital in Choongnam. The parameters for nutritional status are the blood chemistry including plasma protein, albumin, hemoglobin and the anthropometric measurements consisting of weight, BMI, LBM, the proportion of body fat, body fluid and triceps skin fold using bioimpedence analizer and caliper. The results are as follows : 1. The subjects were 55 years and stayed 8 days on average. Of the 100 subjects, males were 61%, neurologic/neurosurgical diseases were 68% and the incidence of pressure sores was 17% mainly occuring within 3days after the admission. 2. The present paralysis(or paraplegia) and edema(arm, leg, trunk) were showed more significantly the subjects with pressure sores than those without pressure sores. 3. Regarding with the nutritional status, the subjects with pressure sores had significantly lower the weight, BMI, LBM, body fluid, albumin than the ones without pressure sores. This results were supported the reports of previous studies that the decreased weight and albumin could be the important predictors of pressure sores. Thereafter we should encourage these factors to be utilized in predicting pressure sores for a comprehensive assessment. Nurse should identify patients at risk of the development of pressure sores, assess their nutritional status and dietary intake at regular intervals.

• Asian-Australasian Journal of Animal Sciences
• /
• 제20권9호
• /
• pp.1349-1353
• /
• 2007

Calcineurin is a calmodulin dependent protein that functions as a regulator of muscle cell growth and function. Agents capable of interacting with calcineurin could have important applications in muscle disease treatment as well as in the improvement of livestock production. Calsarcins comprise a family of muscle-specific calcineurin binding proteins which play an important role in modulating the function of calcineurin in muscle cells. Recently, we described the first two members of the calsarcin family (calsarcin-1 and calsarcin-2) in the pig. Here, we characterized the third member of the calsarcin family, calsarcin-3, which is also expressed specifically in skeletal muscle. However, unlike calsarcin-1 and calsarcin-2, the calsarcin-3 mRNA expression in skeletal muscle kept rising throughout the prenatal and postnatal development periods. In addition, radiation hybrid mapping indicated that porcine calsarcin-3 mapped to the distal end of the q arm of pig chromosome 2 (SSC2). A C/T single nucleotide polymorphism site in exon 5 was genotyped using the denaturing high performance liquid chromatography (DHPLC) method and the allele frequencies at this locus were significantly different among breeds.

• 한국생활과학회지
• /
• 제23권4호
• /
• pp.743-757
• /
• 2014

The purpose of this study was to compare nutrient intakes, blood lipids and bone density of male(n=69) and female(n=71) according to the obesity index by %Fat. The average energy intakes of obesity group was higher than normal group in male & female(p<0.001). The protein and fat intakes among three energy nutrients for male were higher than normal group(p<0.05~0.01), and carbohydrate intake of obesity group in female was higher than the other groups. TG, TC/HDL, blood glucose, blood pressure in male were higher than female(p<0.01~0.001), but HDL in male was lower than female. TC/HDL and diastolic blood pressure of obesity group were higher than normal group in male(p<0.05), but had not significant in female. Blood glucose in male was higher than female(p<0.001). T-score of forearm(=-1.26) and calcaneus(=0.42) for female were lower than male(=-0.63, 0.83), and T-score of calcaneus for obesity group(=1.03) in female was higher than normal group(=0.10). The T-score of forearm for obesity group in female was higher than the other group, but was not significant. These results suggest that ratio among three energy nutrients was kept balance for obesity group, especially, fat must be reduced in male than female, carbohydrate will be reduced in female than male to prevent obesity. Nutritional education for treatment obesity to prevent hyperlipidemia and arteriosclerosis is important for male too. T-score of forearm was lower than calcaneus in female, so exercise with arm than leg would be required to accumulate calcium in bone and to increase muscle to prevent osteoporosis.

• 한국식품영양과학회지
• /
• 제26권5호
• /
• pp.892-900
• /
• 1997

This study was carried out to investigate the clinical characteristics of 78 diabetics by body fat distribution pattern. It was based on the survey of nutritional status, anthropometric measurements and serum components of the patients. The results were as follows: the average ages of male and female subjects were 57.1, and that of female subjects was 58.9, respectively. The average diabetic durations of male and female subjects were 4.8 and 5.9 years, respectively. In male, the ratio of upper body obese subject was 62.5% and the lower body obese subjects was 37.5%, while those of female were 69.4% and 30.6%, respectively. In nutrient intake state, there was no significant difference between male and female subjects, but calory intake of upper body obese subjects was difference between male and female subjects, but calory intake of upper body obese subjects was tend to be higher in both male and female subjects. among the three major nutrients/calory ratio, protein/calory ratio was significantly lower in the male upper body obese subjects than in the male lower body obese subjects. Since weight, circumference of arm and waist, waist hip ratio(WHR) of both male and female subjects, body muscle mass(BMM) of male subjects and body mass index (BMI) of female subjects were significantly higher in upper body obese group, upper body obese subjects represented ore fat than lower body obese subjects. In male subjects, hemoglobin(Hb), A/G of upper body obese subjects were lower than the standard value, but there was no significant difference in the serum components between two subjects. In female subjects, Hb and A/G of lower body obese subjects were lower than those of the normal subjects, but Hb and Ht of upper body obese subjects were significantly higher than those of the lower body obese subjects.

• Journal of Community Nutrition
• /
• 제4권1호
• /
• pp.12-21
• /
• 2002

This study was conducted to investigate the dietary intake, anthropometric data, and association between two factors and main food sources contributing macronutrients for overweight and obese females. Subjects were 85 adult females (overweight : 28, obese 57) where mean age was 38.7y. The results are summarized as follows. Mean fat percent, BMI, obesity rate were 29.3, 23.9 and 15.1%, respectively for overweight women and 32.7, 28.3, 36.4%, respectively for obese women. There were significant differences for most of the anthropometric data between groups. Fat percent for all subjects was significantly correlated with weight, waist circumference, hip circumference, mid arm circumference or skinfold thickness for the triceps, subscapular and suprailiac (p < 0.001). The parameter which showed the highest correlation coefficient (r=0.6156) with fat Percentage was the waist to hip ratio. Any significant differences were not found in dietary intake of nutrients or in diet composition between groups. The mean energy intake was 2090.1kcal (104% of RDA) for overweight women and 2113.0kcal (106% of RDA) for obese women. PFC ratio for overweight was 17 : 24 : 59 and 18 : 23 : 58 for obese subjects, which can be regarded as higher fat and lower carbohydrate percentages compared to recommended PFC ratio (15 20 65) .4) Fat intake was positively correlated (r : 0.2301, p < 0.05) with the triceps skinfold thickness, protein intake was also positively correlated with waist circumference (r=0.2668, p < 0.05) or fat weight(r: 0.2406, p < 0.05) .5) The main food items that contributed to energy intake for overweight or obese subjects were similar (rice, pork, bread, grapes, barley) except com oil in overweight or instant noodle for obese group. The subjects in this study were taking less energy from rice and more energy from pork and bread than women from 98 National Health and Nutrition Survey. Because there were no significant differences of dietary data between overweight and obese group, further investigation considering basal metabolic rate or activity would be needed.

• 대한지역사회영양학회지
• /
• 제2권4호
• /
• pp.568-577
• /
• 1997

To assess the nutritional and health status of the elderly, anthropometric measurements and blood test for analyzing biochemical indices were carried out from August to September in 1996. Data on the incidence of a specific disease and clinical symptoms were also collected by interviews with a questionnaire. Among the total of 169 free-living elderly interviewed(91 men, 78 women), 86 elderly(57 men, 29 women) participated in the anthropometric measurements, and 73 elderly(52 men, 21 women) in blood tests. Average heights and weights were much lower than the standards established in the Korean Recommended Dietary Allowances. While average triceps skinfold thickness of men and women were above the 50 percentile of the reference data, mid-arm muscle circumferences were lower than the 50 percentile. Prevalences of anemia, assessed by hemoglobin using WHO definition, were 38.5% for men and 33.3% for women. Blood levels of total protein and albumin were above the normal limit for all participants. Mean blood cholesterol levels of men and women were 163.9mg/dl and 185.8mg/dl, and triglyceride levels were 138.2mg/dl and 161.9mg/dl, respectively. Women older than 75 years generally had high levels of BMI, total cholesterol and triglyceride. Health status assessed by clinical symptoms generally showed no significant difference by age, and their smoking, and drinking habits. Males and the elderly who exercised regularly had better health status. It was suggested that exercise was a good way to improve health status. (Korean J Community Nutrition 2(4) : 568-577, 1997)

• 한국약용작물학회지
• /
• 제3권1호
• /
• pp.61-65
• /
• 1995

한국에서 재배되고 있는 시호(Bupleurum falcatum L.)의 유전적 계통의 확립을 위해 시호(Bf), 참시호(BfC), 대전 삼도시호(BfJ) 및 수원 삼도시호(BfS)등 4계통을 대상으로 핵형분석을 통해 염색체의 다형현상을 비교하고, SDS PAGE를 이용하여 저장단백질의 양상을 분석하였다. 체세포 염색체 수는 3계통(Bf, BfC, BfJ)에서 2n=20, 1 계통(BfS)에서 2n=26으로 구분되었다. 염색체 1번은 2n=20 계통의 경우 차중부 염색체였으나, 계통 2n=26에서는 중부 염색체로 관찰되어 차이를 보였다. 계통 2n=20에서 2번, 3번 및 5번염색체가 다형현상을 보였으며, 한국에서 재배되고 있는 계통의 핵형은 일본에서 도입되어 재배되고 있는 대전 삼도시호 및 수원 삼도시호와 차이를 보였다. 이러한 결과는 세포유전학적으로 다른 2가지 이상의 계통이 한국에서 재배되고 있음을 보여주는 것이다. 종자에서 추출한 저장 단백질은 45KD 이상의 단백질에서 수적 차이를 보였으며, 2n=20의 계통에서 3개, 2n=26 계통에서 2개의 특이한 밴드가 관찰되었다.

• 한국식품영양과학회지
• /
• 제14권2호
• /
• pp.99-107
• /
• 1985

This study was carried out to investigate the nutritional and physical status of children, aged 11 and 12 years, living in Haenam Koon (living at home) and in Mokpo City (living in an orphan asylum) located in Chonnam during the period of August 2 nd to 9 th in 1984. The mean values of height, chestgirth, arm circumference and head circumference between both groups of living in the rural area and in the city orphan asylum were not statistically different and similar to Korean Growth Standard. Exceptionally, the values of height and weight of male living at home in the rural area were lower than Korean Growth Standard (p<0.01). The weight of male living in the city orphan asylum was higher han that of male living at home in the rural area (p<0.01), and the skin fold thickness of female living at home was higher than that of male living at home (p <0.01). The physical indices of children living in the city orphan asylum were more or less higher than those of living at home in the rural area, but the relative weight of male was only significant (p<0.05). The animal protein intake was $8.4{\sim}8.6%$ of total food intake of living at home in the rural area and 6.2% in living in the city orphan asylum. The intake of legumes of children living in the city orphan asylum was more two times than that of living at home in the rural area, but overall fruit intake of living at home was more about two times than that of living in the city orphan asylum. The average intake of all nutrients except thiamine and niacin was lower than RDA. Especially the calcium intake of children in both groups was the lowest of all nutrients ($28.9{\sim}40.6%$ of RDA). Children living at home in the rural area had significantly higher intake of fat and ascorbic acid than children living in the city orphan asylum (p<0.01).